Influence of heat stress on in vitro oocyte and embryo production in high-yielding Holstein cows
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/71852 |
Resumo: | The objective of this study was to evaluate the influence of thermal shock on oocytes used in the production of in vitro embryos (IVP) of high productivity Holstein cows on the day of follicular aspiration (OPU; 0), 30, 60 and 90 days before the OPU. From the mean temperature on day 0 and on the previous 30, 60 and 90 days, they were classified into comfort group (TC; up to 15°C) and heat stress (HS; above 15°C) groups. A negative influence was observed on oocytes and viable embryos (total and grade I). The heat stress in the periods of 30 and 60 days prior to OPU resulted in lower production of viable oocytes (P=0.0028; P=0.0092, respectively). Under stress, on the day of OPU (HS-OPU), cows showed no reduction in the amount of viable oocytes (P=0.5497) and there was no influence of temperature for the group stressed 90 days before OPU (P=0.8287). For total embryos, the difference occurred only in the HS-30 group (P=0.0317), where the groups HS-OPU, HS-60, HS-90 presented, respectively, P=0. 1987, P=0.0596 and P=0.4580. Regarding the production of embryos of grade 1, there was no difference for the groups HS-OPU (P=0.2291) and HS-90 (P=0.2868), but there was a reduction for HS-30 (P=0.0143) and HS-60 (P=0.0253). In summary, heat stress had a negative impact when it occurred 30 or 60 days before follicular aspiration. In addition, 30 days seems to be the period of more susceptibility and that causes the greatest deleterious effects on oocyte viability and IVP.Keywords: Hyperthermia; Thermal shock; Ovum Pick Up; Dairy cows. |
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Influence of heat stress on in vitro oocyte and embryo production in high-yielding Holstein cowsInfluência do estresse calórico na produção in vitro de oócitos e embriões de vacas Holandesas de alta produtividadeThe objective of this study was to evaluate the influence of thermal shock on oocytes used in the production of in vitro embryos (IVP) of high productivity Holstein cows on the day of follicular aspiration (OPU; 0), 30, 60 and 90 days before the OPU. From the mean temperature on day 0 and on the previous 30, 60 and 90 days, they were classified into comfort group (TC; up to 15°C) and heat stress (HS; above 15°C) groups. A negative influence was observed on oocytes and viable embryos (total and grade I). The heat stress in the periods of 30 and 60 days prior to OPU resulted in lower production of viable oocytes (P=0.0028; P=0.0092, respectively). Under stress, on the day of OPU (HS-OPU), cows showed no reduction in the amount of viable oocytes (P=0.5497) and there was no influence of temperature for the group stressed 90 days before OPU (P=0.8287). For total embryos, the difference occurred only in the HS-30 group (P=0.0317), where the groups HS-OPU, HS-60, HS-90 presented, respectively, P=0. 1987, P=0.0596 and P=0.4580. Regarding the production of embryos of grade 1, there was no difference for the groups HS-OPU (P=0.2291) and HS-90 (P=0.2868), but there was a reduction for HS-30 (P=0.0143) and HS-60 (P=0.0253). In summary, heat stress had a negative impact when it occurred 30 or 60 days before follicular aspiration. In addition, 30 days seems to be the period of more susceptibility and that causes the greatest deleterious effects on oocyte viability and IVP.Keywords: Hyperthermia; Thermal shock; Ovum Pick Up; Dairy cows. Objetivou-se avaliar a influência do estresse térmico em oócitos utilizados na produção in vitro de embriões (PIV) bovinos da raça Holandesa de alta produtividade no dia da aspiração folicular (OPU; 0), 30, 60 e 90 dias antes da OPU. A partir da temperatura média no dia 0 e aos 30, 60 e 90 dias anteriores, foram classificados nos grupos conforto (CT; até 15°C) e estresse por calor (ET - acima de 15°C). Observou-se influência negativa em oócitos e embriões viáveis (total e grau I). A submissão ao estresse térmico nos períodos de 30 e 60 dias anteriores à OPU resultou em menor produção de oócitos viáveis (P=0,0028; P=0,0092, respectivamente). Sob estresse, no dia da OPU (ET-OPU), as vacas não apresentaram redução na quantidade de oócitos viáveis (P=0,5497) e não houve influência da temperatura para o grupo estressado 90 dias antes da OPU (P=0,8287). Para embriões totais, a diferença ocorreu apenas no grupo ET-30 (P=0,0317), onde os grupos ET-OPU, ET-60, ET-90 apresentaram, respectivamente, P=0,1987, P=0,0596 e P=0,4580. Em relação à produção de embriões grau 1, não houve diferença para os grupos ET-OPU (P=0,2291) e ET-90 (P=0,2868), porém houve redução para ET-30 (P=0,0143) e ET- 60 (P=0,0253). Em resumo, o estresse por calor teve impacto negativo quando ocorreu 30 ou 60 dias antes da aspiração folicular. Além disso, 30 dias parece ser o período de maior suscetibilidade e que causa os maiores efeitos deletérios na viabilidade oocitária e na PIV.Palavras-chave: Hipertermia; Estresse térmico; Aspiração Folicular; Vacas leiteiras.Universidade Federal de Goiás2022-07-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://revistas.ufg.br/vet/article/view/71852Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 23 (2022): Continuous publicationCiência Animal Brasileira / Brazilian Animal Science; v. 23 (2022): Publicação contínua1809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporenghttps://revistas.ufg.br/vet/article/view/71852/38470https://revistas.ufg.br/vet/article/view/71852/38564https://revistas.ufg.br/vet/article/view/71852/38471https://revistas.ufg.br/vet/article/view/71852/38565Copyright (c) 2022 Ciência Animal Brasileira / Brazilian Animal Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBerling, FrancieliCavallari de Castro, Fernanda dos Santos Oliveira, Ana Carolina 2023-03-23T18:43:54Zoai:ojs.revistas.ufg.br:article/71852Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:30.146992Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
Influence of heat stress on in vitro oocyte and embryo production in high-yielding Holstein cows Influência do estresse calórico na produção in vitro de oócitos e embriões de vacas Holandesas de alta produtividade |
title |
Influence of heat stress on in vitro oocyte and embryo production in high-yielding Holstein cows |
spellingShingle |
Influence of heat stress on in vitro oocyte and embryo production in high-yielding Holstein cows Berling, Francieli |
title_short |
Influence of heat stress on in vitro oocyte and embryo production in high-yielding Holstein cows |
title_full |
Influence of heat stress on in vitro oocyte and embryo production in high-yielding Holstein cows |
title_fullStr |
Influence of heat stress on in vitro oocyte and embryo production in high-yielding Holstein cows |
title_full_unstemmed |
Influence of heat stress on in vitro oocyte and embryo production in high-yielding Holstein cows |
title_sort |
Influence of heat stress on in vitro oocyte and embryo production in high-yielding Holstein cows |
author |
Berling, Francieli |
author_facet |
Berling, Francieli Cavallari de Castro, Fernanda dos Santos Oliveira, Ana Carolina |
author_role |
author |
author2 |
Cavallari de Castro, Fernanda dos Santos Oliveira, Ana Carolina |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Berling, Francieli Cavallari de Castro, Fernanda dos Santos Oliveira, Ana Carolina |
description |
The objective of this study was to evaluate the influence of thermal shock on oocytes used in the production of in vitro embryos (IVP) of high productivity Holstein cows on the day of follicular aspiration (OPU; 0), 30, 60 and 90 days before the OPU. From the mean temperature on day 0 and on the previous 30, 60 and 90 days, they were classified into comfort group (TC; up to 15°C) and heat stress (HS; above 15°C) groups. A negative influence was observed on oocytes and viable embryos (total and grade I). The heat stress in the periods of 30 and 60 days prior to OPU resulted in lower production of viable oocytes (P=0.0028; P=0.0092, respectively). Under stress, on the day of OPU (HS-OPU), cows showed no reduction in the amount of viable oocytes (P=0.5497) and there was no influence of temperature for the group stressed 90 days before OPU (P=0.8287). For total embryos, the difference occurred only in the HS-30 group (P=0.0317), where the groups HS-OPU, HS-60, HS-90 presented, respectively, P=0. 1987, P=0.0596 and P=0.4580. Regarding the production of embryos of grade 1, there was no difference for the groups HS-OPU (P=0.2291) and HS-90 (P=0.2868), but there was a reduction for HS-30 (P=0.0143) and HS-60 (P=0.0253). In summary, heat stress had a negative impact when it occurred 30 or 60 days before follicular aspiration. In addition, 30 days seems to be the period of more susceptibility and that causes the greatest deleterious effects on oocyte viability and IVP.Keywords: Hyperthermia; Thermal shock; Ovum Pick Up; Dairy cows. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/71852 |
url |
https://revistas.ufg.br/vet/article/view/71852 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/71852/38470 https://revistas.ufg.br/vet/article/view/71852/38564 https://revistas.ufg.br/vet/article/view/71852/38471 https://revistas.ufg.br/vet/article/view/71852/38565 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Ciência Animal Brasileira / Brazilian Animal Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Ciência Animal Brasileira / Brazilian Animal Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 23 (2022): Continuous publication Ciência Animal Brasileira / Brazilian Animal Science; v. 23 (2022): Publicação contínua 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
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1799874790641631232 |