OCCURRENCE OF GENES ENCODING STAPHYLOCOCCAL ENTEROTOXINS ISOLATED FROM COW´S MILK SAMPLES

Detalhes bibliográficos
Autor(a) principal: Chaves Lúcio, Érica
Data de Publicação: 2018
Outros Autores: Silva Albuquerque, Milena, Mário Baltazar Oliveira, Júnior, Veneroni Gouveia, Gisele, Matiuzzi Costa, Mateus, Aparecido Mota, Rinaldo, Wilton Pinheiro Junior, José
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/e-43108
Resumo: The objective of this study was to identify the occurrence of genes expressing staphylococcal enterotoxin (sea, seb, sec, and seg) and the toxin gene 1 of toxic shock syndrome (tst) in Staphylococcus aureus coming from cases of bovine mastitis in the state of Pernambuco, Brazil. We analyzed 93 isolates and we observed the presence of toxigenic gene in 20 (21.6%) samples, of which 11 (55.0%) were positive for tst gene, seven (35.0%) for the sec gene, and two (10.0%) for the seg gene. Of the 20 isolates amplified in PCR for the presence of the sec gene, seg and tst, 16 (80.0%) were positive for only one gene and four (20.0%) were positive for two genes (sec and tst). Of the 17 properties from which the samples originated, seven (41.2%) had positive samples for at least one of the genes sec, seg, and tst. This is the first record of the occurrence of the genes encoding the enterotoxin SEC and TST-1 in milk samples from cows with mastitis in the state of Pernambuco, Brazil.Keywords: Mastitis; Pathogenesis; PCR; Staphylococcus aureus
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spelling OCCURRENCE OF GENES ENCODING STAPHYLOCOCCAL ENTEROTOXINS ISOLATED FROM COW´S MILK SAMPLESOcorrência de genes codificadores de enterotoxinas estafilocócicas em amostras de leite de vacasThe objective of this study was to identify the occurrence of genes expressing staphylococcal enterotoxin (sea, seb, sec, and seg) and the toxin gene 1 of toxic shock syndrome (tst) in Staphylococcus aureus coming from cases of bovine mastitis in the state of Pernambuco, Brazil. We analyzed 93 isolates and we observed the presence of toxigenic gene in 20 (21.6%) samples, of which 11 (55.0%) were positive for tst gene, seven (35.0%) for the sec gene, and two (10.0%) for the seg gene. Of the 20 isolates amplified in PCR for the presence of the sec gene, seg and tst, 16 (80.0%) were positive for only one gene and four (20.0%) were positive for two genes (sec and tst). Of the 17 properties from which the samples originated, seven (41.2%) had positive samples for at least one of the genes sec, seg, and tst. This is the first record of the occurrence of the genes encoding the enterotoxin SEC and TST-1 in milk samples from cows with mastitis in the state of Pernambuco, Brazil.Keywords: Mastitis; Pathogenesis; PCR; Staphylococcus aureusObjetivou-se com este trabalho identificar a ocorrência de genes codificadores de enterotoxinas estafilocócicas (sea, seb, sec e seg) e do gene da toxina 1 responsável pela síndrome do choque tóxico (tst) em isolados de Staphylococcus aureus procedentes de casos de mastite bovina, no estado de Pernambuco, Brasil. Foram analisados 93 isolados e observou-se a presença de genes toxigênicos em 20 (21,6%) deles, dos quais 11 (55,0%) foram positivos para o gene tst, sete (35,0%) para o gene sec e dois (10,0%) para o gene seg. Dentre os 20 isolados que amplificaram na PCR para presença dos genes sec, seg e tst, 16 (80,0%) foram positivos apenas para um gene e quatro (20,0%) foram positivos para dois genes (sec e tst). Das 17 propriedades de onde as amostras tiveram origem, sete (41,2%) apresentaram amostras positivas para pelo menos um dos genes sec, seg e tst. Este é primeiro registro de ocorrência dos genes codificadores das enterotoxinas SEC e TST-1 em amostras de leite de vacas com mastite no estado de Pernambuco, Brasil.Palavras-chave: Mastite; Patogênese; PCR; Staphylococcus aureusUniversidade Federal de Goiás2018-07-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/e-4310810.1590/cab19043108Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-8Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-81809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/e-43108/25784https://revistas.ufg.br/vet/article/view/e-43108/25785Copyright (c) 2018 Ciência Animal Brasileirainfo:eu-repo/semantics/openAccessChaves Lúcio, ÉricaSilva Albuquerque, MilenaMário Baltazar Oliveira, JúniorVeneroni Gouveia, GiseleMatiuzzi Costa, MateusAparecido Mota, RinaldoWilton Pinheiro Junior, José2023-03-23T18:50:39Zoai:ojs.revistas.ufg.br:article/43108Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:15.612795Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv OCCURRENCE OF GENES ENCODING STAPHYLOCOCCAL ENTEROTOXINS ISOLATED FROM COW´S MILK SAMPLES
Ocorrência de genes codificadores de enterotoxinas estafilocócicas em amostras de leite de vacas
title OCCURRENCE OF GENES ENCODING STAPHYLOCOCCAL ENTEROTOXINS ISOLATED FROM COW´S MILK SAMPLES
spellingShingle OCCURRENCE OF GENES ENCODING STAPHYLOCOCCAL ENTEROTOXINS ISOLATED FROM COW´S MILK SAMPLES
Chaves Lúcio, Érica
title_short OCCURRENCE OF GENES ENCODING STAPHYLOCOCCAL ENTEROTOXINS ISOLATED FROM COW´S MILK SAMPLES
title_full OCCURRENCE OF GENES ENCODING STAPHYLOCOCCAL ENTEROTOXINS ISOLATED FROM COW´S MILK SAMPLES
title_fullStr OCCURRENCE OF GENES ENCODING STAPHYLOCOCCAL ENTEROTOXINS ISOLATED FROM COW´S MILK SAMPLES
title_full_unstemmed OCCURRENCE OF GENES ENCODING STAPHYLOCOCCAL ENTEROTOXINS ISOLATED FROM COW´S MILK SAMPLES
title_sort OCCURRENCE OF GENES ENCODING STAPHYLOCOCCAL ENTEROTOXINS ISOLATED FROM COW´S MILK SAMPLES
author Chaves Lúcio, Érica
author_facet Chaves Lúcio, Érica
Silva Albuquerque, Milena
Mário Baltazar Oliveira, Júnior
Veneroni Gouveia, Gisele
Matiuzzi Costa, Mateus
Aparecido Mota, Rinaldo
Wilton Pinheiro Junior, José
author_role author
author2 Silva Albuquerque, Milena
Mário Baltazar Oliveira, Júnior
Veneroni Gouveia, Gisele
Matiuzzi Costa, Mateus
Aparecido Mota, Rinaldo
Wilton Pinheiro Junior, José
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Chaves Lúcio, Érica
Silva Albuquerque, Milena
Mário Baltazar Oliveira, Júnior
Veneroni Gouveia, Gisele
Matiuzzi Costa, Mateus
Aparecido Mota, Rinaldo
Wilton Pinheiro Junior, José
description The objective of this study was to identify the occurrence of genes expressing staphylococcal enterotoxin (sea, seb, sec, and seg) and the toxin gene 1 of toxic shock syndrome (tst) in Staphylococcus aureus coming from cases of bovine mastitis in the state of Pernambuco, Brazil. We analyzed 93 isolates and we observed the presence of toxigenic gene in 20 (21.6%) samples, of which 11 (55.0%) were positive for tst gene, seven (35.0%) for the sec gene, and two (10.0%) for the seg gene. Of the 20 isolates amplified in PCR for the presence of the sec gene, seg and tst, 16 (80.0%) were positive for only one gene and four (20.0%) were positive for two genes (sec and tst). Of the 17 properties from which the samples originated, seven (41.2%) had positive samples for at least one of the genes sec, seg, and tst. This is the first record of the occurrence of the genes encoding the enterotoxin SEC and TST-1 in milk samples from cows with mastitis in the state of Pernambuco, Brazil.Keywords: Mastitis; Pathogenesis; PCR; Staphylococcus aureus
publishDate 2018
dc.date.none.fl_str_mv 2018-07-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/e-43108
10.1590/cab19043108
url https://revistas.ufg.br/vet/article/view/e-43108
identifier_str_mv 10.1590/cab19043108
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/e-43108/25784
https://revistas.ufg.br/vet/article/view/e-43108/25785
dc.rights.driver.fl_str_mv Copyright (c) 2018 Ciência Animal Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Ciência Animal Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-8
Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-8
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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