CHARACTERIZATION OF Aeromonas spp ISOLATED FROM WATER AND OF OYSTERS SAMPLES BY MICROBIOLOGICAL AND MOLECULAR METHODS

Detalhes bibliográficos
Autor(a) principal: Silva, Ana Carolina Miranda de Melo
Data de Publicação: 2014
Outros Autores: Nascimento, Danielle Lopes do, Machado, Rosâgela Zacarias, Costa, Francisca Neide
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/28351
Resumo: This study aimed to isolate and identify Aeromonas bacteria from samples of oysters and water, by microbiological and molecular methods. Identification of Aeromonas was performed by the conventional method and by PCR and species characterization was performed by identification key Aerokey II and by RFLP-PCR of 16S rDNA. 59 (98.3%) samples of oysters and 15 (75%) samples of water contaminated by Aeromonas bacteria were identified. Of the 74 isolates of Aeromonas spp. obtained by microbiological analysis, 53 (71.62%), 38 (65.51%) oyster samples and 15 (100%) of water samples confirmed the characterization of the genus by molecular technique. As to biochemical species identification, 59.3% (n = 35) and 40.6% (n = 24) of isolates from Aeromonas sp. obtained from oyster samples were rated as A. hydrophila and A. caviae, respectively; and from isolates obtained from water samples, 60% (n = 9) were classified as A. hydrophila and 40% (n = 6) as A. caviae; however, only the A. hydrophila was confirmed by genetic sequencing. Regarding the classification by RFLP-PCR 16S rDNA, patterns of nonspecific bands were obtained, making it impossible to identify the species by this technique. The large number of oyester samples contaminated with Aeromonas spp. and identification of A. hydrophila, demonstrate that this mollusk might present a risk to consumer health, mainly because it is consumed raw and is to a potentially pathogenic micro-organism to humans. KEYWORDS: Aeromonas, oysters, PCR, water.
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spelling CHARACTERIZATION OF Aeromonas spp ISOLATED FROM WATER AND OF OYSTERS SAMPLES BY MICROBIOLOGICAL AND MOLECULAR METHODSCARACTERIZAÇÃO DE Aeromonas spp ISOLADAS DE AMOSTRAS DE OSTRAS E ÁGUA POR MÉTODO MICROBIOLÓGICO E MOLECULARFood TechnologyMicrobiologia e Biologia MolecularThis study aimed to isolate and identify Aeromonas bacteria from samples of oysters and water, by microbiological and molecular methods. Identification of Aeromonas was performed by the conventional method and by PCR and species characterization was performed by identification key Aerokey II and by RFLP-PCR of 16S rDNA. 59 (98.3%) samples of oysters and 15 (75%) samples of water contaminated by Aeromonas bacteria were identified. Of the 74 isolates of Aeromonas spp. obtained by microbiological analysis, 53 (71.62%), 38 (65.51%) oyster samples and 15 (100%) of water samples confirmed the characterization of the genus by molecular technique. As to biochemical species identification, 59.3% (n = 35) and 40.6% (n = 24) of isolates from Aeromonas sp. obtained from oyster samples were rated as A. hydrophila and A. caviae, respectively; and from isolates obtained from water samples, 60% (n = 9) were classified as A. hydrophila and 40% (n = 6) as A. caviae; however, only the A. hydrophila was confirmed by genetic sequencing. Regarding the classification by RFLP-PCR 16S rDNA, patterns of nonspecific bands were obtained, making it impossible to identify the species by this technique. The large number of oyester samples contaminated with Aeromonas spp. and identification of A. hydrophila, demonstrate that this mollusk might present a risk to consumer health, mainly because it is consumed raw and is to a potentially pathogenic micro-organism to humans. KEYWORDS: Aeromonas, oysters, PCR, water.Objetivou-se isolar e identificar bactérias do gênero Aeromonas de amostras de ostras e de água, pelos métodos microbiológico e molecular. A identificação do gênero Aeromonas foi realizada pelo método convencional e pela técnica da PCR e a caracterização das espécies foi realizada pela chave de identificação Aerokey II e pela técnica de RFLP-PCR do 16S rDNA. Foram identificadas 59 (98,3%) amostras de ostras e 15 (75%) amostras de água contaminadas por bactérias do gênero Aeromonas. Dos 74 isolados de Aeromonas spp. obtidos pela análise microbiológica, 53 (71,62%), 38 (65,51%) de amostras de ostras e 15 (100%) de amostras de água, confirmaram a caracterização do gênero pela técnica molecular. Quanto a identificação bioquímica das espécies 59,3% (n=35) e 40,6% (n=24) dos isolados de Aeromonas sp. obtidos das amostras de ostras foram classificados como A. hydrophila e A. caviae, respectivamente; e para os isolados obtidos das amostras de água, 60% (n=9) foram classificados como A. hydrophila e 40% (n=6) como A. caviae; entretanto, somente a A. hydrophila foi confirmada pelo sequenciamento genético. Quanto a classificação através da RFLP-PCR 16S rDNA, foram obtidos padrões de bandas inespecíficos, impossibilitando a identificação das espécies por esta técnica. O grande número de amostras de ostras contaminadas com Aeromonas spp. e a identificação da A. hydrophila demonstram que este molusco pode oferecer risco à saúde dos consumidores, principalmente por ser consumido in natura e se tratar de um micro-organismo potencialmente patogênico para o ser humano.PALAVRAS-CHAVE: água, Aeromonas, ostras, PCR. Universidade Federal de Goiás2014-09-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2835110.5216/cab.v15i3.28351Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 3 (2014); 362-368Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 3 (2014); 362-3681809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/28351/17094https://revistas.ufg.br/vet/article/view/28351/17095Silva, Ana Carolina Miranda de MeloNascimento, Danielle Lopes doMachado, Rosâgela ZacariasCosta, Francisca Neideinfo:eu-repo/semantics/openAccess2014-09-29T12:57:42Zoai:ojs.revistas.ufg.br:article/28351Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2014-09-29T12:57:42Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)false
dc.title.none.fl_str_mv CHARACTERIZATION OF Aeromonas spp ISOLATED FROM WATER AND OF OYSTERS SAMPLES BY MICROBIOLOGICAL AND MOLECULAR METHODS
CARACTERIZAÇÃO DE Aeromonas spp ISOLADAS DE AMOSTRAS DE OSTRAS E ÁGUA POR MÉTODO MICROBIOLÓGICO E MOLECULAR
title CHARACTERIZATION OF Aeromonas spp ISOLATED FROM WATER AND OF OYSTERS SAMPLES BY MICROBIOLOGICAL AND MOLECULAR METHODS
spellingShingle CHARACTERIZATION OF Aeromonas spp ISOLATED FROM WATER AND OF OYSTERS SAMPLES BY MICROBIOLOGICAL AND MOLECULAR METHODS
Silva, Ana Carolina Miranda de Melo
Food Technology
Microbiologia e Biologia Molecular
title_short CHARACTERIZATION OF Aeromonas spp ISOLATED FROM WATER AND OF OYSTERS SAMPLES BY MICROBIOLOGICAL AND MOLECULAR METHODS
title_full CHARACTERIZATION OF Aeromonas spp ISOLATED FROM WATER AND OF OYSTERS SAMPLES BY MICROBIOLOGICAL AND MOLECULAR METHODS
title_fullStr CHARACTERIZATION OF Aeromonas spp ISOLATED FROM WATER AND OF OYSTERS SAMPLES BY MICROBIOLOGICAL AND MOLECULAR METHODS
title_full_unstemmed CHARACTERIZATION OF Aeromonas spp ISOLATED FROM WATER AND OF OYSTERS SAMPLES BY MICROBIOLOGICAL AND MOLECULAR METHODS
title_sort CHARACTERIZATION OF Aeromonas spp ISOLATED FROM WATER AND OF OYSTERS SAMPLES BY MICROBIOLOGICAL AND MOLECULAR METHODS
author Silva, Ana Carolina Miranda de Melo
author_facet Silva, Ana Carolina Miranda de Melo
Nascimento, Danielle Lopes do
Machado, Rosâgela Zacarias
Costa, Francisca Neide
author_role author
author2 Nascimento, Danielle Lopes do
Machado, Rosâgela Zacarias
Costa, Francisca Neide
author2_role author
author
author
dc.contributor.author.fl_str_mv Silva, Ana Carolina Miranda de Melo
Nascimento, Danielle Lopes do
Machado, Rosâgela Zacarias
Costa, Francisca Neide
dc.subject.por.fl_str_mv Food Technology
Microbiologia e Biologia Molecular
topic Food Technology
Microbiologia e Biologia Molecular
description This study aimed to isolate and identify Aeromonas bacteria from samples of oysters and water, by microbiological and molecular methods. Identification of Aeromonas was performed by the conventional method and by PCR and species characterization was performed by identification key Aerokey II and by RFLP-PCR of 16S rDNA. 59 (98.3%) samples of oysters and 15 (75%) samples of water contaminated by Aeromonas bacteria were identified. Of the 74 isolates of Aeromonas spp. obtained by microbiological analysis, 53 (71.62%), 38 (65.51%) oyster samples and 15 (100%) of water samples confirmed the characterization of the genus by molecular technique. As to biochemical species identification, 59.3% (n = 35) and 40.6% (n = 24) of isolates from Aeromonas sp. obtained from oyster samples were rated as A. hydrophila and A. caviae, respectively; and from isolates obtained from water samples, 60% (n = 9) were classified as A. hydrophila and 40% (n = 6) as A. caviae; however, only the A. hydrophila was confirmed by genetic sequencing. Regarding the classification by RFLP-PCR 16S rDNA, patterns of nonspecific bands were obtained, making it impossible to identify the species by this technique. The large number of oyester samples contaminated with Aeromonas spp. and identification of A. hydrophila, demonstrate that this mollusk might present a risk to consumer health, mainly because it is consumed raw and is to a potentially pathogenic micro-organism to humans. KEYWORDS: Aeromonas, oysters, PCR, water.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/28351
10.5216/cab.v15i3.28351
url https://revistas.ufg.br/vet/article/view/28351
identifier_str_mv 10.5216/cab.v15i3.28351
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/28351/17094
https://revistas.ufg.br/vet/article/view/28351/17095
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 3 (2014); 362-368
Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 3 (2014); 362-368
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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