QUALITY OF TAMBAQUI (Colossoma macropomum) SEMEN CRYOPRESERVED IN DIFFERENT CONCENTRATIONS OF EGG YOLK
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/25386 |
Resumo: | The aim of this study was to evaluate the effect of egg yolk (EY) addition on the sperm kinects after cryopreservation. We used twenty tambaqui males (n = 4 pools), which were induced homonally to spermiation with carp pituitary extract. Fourteen hours after induction, a seminal collection was performed. The semen from each pool was diluted with Ringer added 10% DMSO on the presence or absence of egg yolk (T1: no additional EY; T2: 5% EY; and T3: 10% EY). The treated semen was loaded in 0.5 mL straws, frozen in a nitrogen vapor vessel (dry shipper) (30 min / -153 °C), and then transferred to liquid nitrogen. Straws were thawed in a water bath at 37 °C / 30 seconds. The analyses of motility rate (%) and sperm curvilinear velocity (µm/s) were performed on computerized system (CASA). Data were expressed as mean ± standard deviation, and Tukey test was applied (P<0.05). There was a significant reduction in the percentage of motile spermatozoa and curvilinear velocity after the addition of egg yolk to the crioprotection solution, regardless of the concentration. Therefore, the addition of EY to Ringer + DMSO had negative effect on motility of tambaqui frozen sperm.Keywords: extracellular cryoprotectant; freezing; reproduction. |
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Ciência animal brasileira (Online) |
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QUALITY OF TAMBAQUI (Colossoma macropomum) SEMEN CRYOPRESERVED IN DIFFERENT CONCENTRATIONS OF EGG YOLKQUALIDADE DO SÊMEN DE TAMBAQUI (Colossoma macropomum) CRIOPRESERVADO EM DIFERENTES CONCENTRAÇÕES DE GEMA DE OVOAnimal Reproductioncongelaçãocrioprotetor externoreproduçãoThe aim of this study was to evaluate the effect of egg yolk (EY) addition on the sperm kinects after cryopreservation. We used twenty tambaqui males (n = 4 pools), which were induced homonally to spermiation with carp pituitary extract. Fourteen hours after induction, a seminal collection was performed. The semen from each pool was diluted with Ringer added 10% DMSO on the presence or absence of egg yolk (T1: no additional EY; T2: 5% EY; and T3: 10% EY). The treated semen was loaded in 0.5 mL straws, frozen in a nitrogen vapor vessel (dry shipper) (30 min / -153 °C), and then transferred to liquid nitrogen. Straws were thawed in a water bath at 37 °C / 30 seconds. The analyses of motility rate (%) and sperm curvilinear velocity (µm/s) were performed on computerized system (CASA). Data were expressed as mean ± standard deviation, and Tukey test was applied (P<0.05). There was a significant reduction in the percentage of motile spermatozoa and curvilinear velocity after the addition of egg yolk to the crioprotection solution, regardless of the concentration. Therefore, the addition of EY to Ringer + DMSO had negative effect on motility of tambaqui frozen sperm.Keywords: extracellular cryoprotectant; freezing; reproduction.O objetivo deste trabalho foi avaliar o efeito da adição de gema de ovo (GO) sobre a cinética dos espermatozoides de tambaquis após a criopreservação. Utilizaram-se vinte machos de tambaquis (n= 4 pools), que foram induzidos hormonalmente com extrato hipofisário de carpa, para espermiação. Quatorze horas após a indução, realizou-se a coleta seminal. O sêmen de cada pool foi diluído em Ringer adicionado de 10% de dimetilsulfóxido (DMSO) acrescido ou não de GO (T1: sem acréscimo de GO; T2: com 5% de GO e T3: com 10% de GO). O sêmen tratado foi envasado em palhetas de 0,5 mL, congelado em vapor de nitrogênio líquido (dry shipper-30 min/-153 °C) e posteriormente transferidas para nitrogênio líquido. As palhetas foram descongeladas em banho-maria a 37 °C/30 segundos. A taxa de motilidade (%) e a velocidade curvilinear espermática (µm/s) foram analisadas em sistema computadorizado (CASA). Os dados foram expressos em média ± desvio padrão e foi aplicado o teste de Tukey (P<0,05). Houve uma redução significativa na porcentagem de espermatozoides móveis e velocidade curvilinear após a adição de GO independente da concentração. Logo, a adição de GO ao Ringer + DMSO teve efeito negativo sobre a motilidade do sêmen congelado de tambaqui. Palavras-chave: congelação; crioprotetor extracelular; reprodução.Universidade Federal de Goiás2016-04-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2538610.1590/cab17225386Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 17 No. 2 (2016); 267-273Ciência Animal Brasileira / Brazilian Animal Science; v. 17 n. 2 (2016); 267-2731809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/25386/20924https://revistas.ufg.br/vet/article/view/25386/20925Pinheiro, João Paulo SilvaLeite-Castro, Liliane VerasOliveira, Fátima de Cássia Evangelista deLinhares, Francisco Renan AragãoLopes, Júlia TrugílioSalmito-Vanderley, Carminda Sandra Britoinfo:eu-repo/semantics/openAccess2016-04-29T11:36:30Zoai:ojs.revistas.ufg.br:article/25386Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:00.851954Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
QUALITY OF TAMBAQUI (Colossoma macropomum) SEMEN CRYOPRESERVED IN DIFFERENT CONCENTRATIONS OF EGG YOLK QUALIDADE DO SÊMEN DE TAMBAQUI (Colossoma macropomum) CRIOPRESERVADO EM DIFERENTES CONCENTRAÇÕES DE GEMA DE OVO |
title |
QUALITY OF TAMBAQUI (Colossoma macropomum) SEMEN CRYOPRESERVED IN DIFFERENT CONCENTRATIONS OF EGG YOLK |
spellingShingle |
QUALITY OF TAMBAQUI (Colossoma macropomum) SEMEN CRYOPRESERVED IN DIFFERENT CONCENTRATIONS OF EGG YOLK Pinheiro, João Paulo Silva Animal Reproduction congelação crioprotetor externo reprodução |
title_short |
QUALITY OF TAMBAQUI (Colossoma macropomum) SEMEN CRYOPRESERVED IN DIFFERENT CONCENTRATIONS OF EGG YOLK |
title_full |
QUALITY OF TAMBAQUI (Colossoma macropomum) SEMEN CRYOPRESERVED IN DIFFERENT CONCENTRATIONS OF EGG YOLK |
title_fullStr |
QUALITY OF TAMBAQUI (Colossoma macropomum) SEMEN CRYOPRESERVED IN DIFFERENT CONCENTRATIONS OF EGG YOLK |
title_full_unstemmed |
QUALITY OF TAMBAQUI (Colossoma macropomum) SEMEN CRYOPRESERVED IN DIFFERENT CONCENTRATIONS OF EGG YOLK |
title_sort |
QUALITY OF TAMBAQUI (Colossoma macropomum) SEMEN CRYOPRESERVED IN DIFFERENT CONCENTRATIONS OF EGG YOLK |
author |
Pinheiro, João Paulo Silva |
author_facet |
Pinheiro, João Paulo Silva Leite-Castro, Liliane Veras Oliveira, Fátima de Cássia Evangelista de Linhares, Francisco Renan Aragão Lopes, Júlia Trugílio Salmito-Vanderley, Carminda Sandra Brito |
author_role |
author |
author2 |
Leite-Castro, Liliane Veras Oliveira, Fátima de Cássia Evangelista de Linhares, Francisco Renan Aragão Lopes, Júlia Trugílio Salmito-Vanderley, Carminda Sandra Brito |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pinheiro, João Paulo Silva Leite-Castro, Liliane Veras Oliveira, Fátima de Cássia Evangelista de Linhares, Francisco Renan Aragão Lopes, Júlia Trugílio Salmito-Vanderley, Carminda Sandra Brito |
dc.subject.por.fl_str_mv |
Animal Reproduction congelação crioprotetor externo reprodução |
topic |
Animal Reproduction congelação crioprotetor externo reprodução |
description |
The aim of this study was to evaluate the effect of egg yolk (EY) addition on the sperm kinects after cryopreservation. We used twenty tambaqui males (n = 4 pools), which were induced homonally to spermiation with carp pituitary extract. Fourteen hours after induction, a seminal collection was performed. The semen from each pool was diluted with Ringer added 10% DMSO on the presence or absence of egg yolk (T1: no additional EY; T2: 5% EY; and T3: 10% EY). The treated semen was loaded in 0.5 mL straws, frozen in a nitrogen vapor vessel (dry shipper) (30 min / -153 °C), and then transferred to liquid nitrogen. Straws were thawed in a water bath at 37 °C / 30 seconds. The analyses of motility rate (%) and sperm curvilinear velocity (µm/s) were performed on computerized system (CASA). Data were expressed as mean ± standard deviation, and Tukey test was applied (P<0.05). There was a significant reduction in the percentage of motile spermatozoa and curvilinear velocity after the addition of egg yolk to the crioprotection solution, regardless of the concentration. Therefore, the addition of EY to Ringer + DMSO had negative effect on motility of tambaqui frozen sperm.Keywords: extracellular cryoprotectant; freezing; reproduction. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Avaliado por pares Pesquisa Científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/25386 10.1590/cab17225386 |
url |
https://revistas.ufg.br/vet/article/view/25386 |
identifier_str_mv |
10.1590/cab17225386 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/25386/20924 https://revistas.ufg.br/vet/article/view/25386/20925 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 17 No. 2 (2016); 267-273 Ciência Animal Brasileira / Brazilian Animal Science; v. 17 n. 2 (2016); 267-273 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874788201594880 |