COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/28163 |
Resumo: | The demand and consumption of fish and their derivatives has increased considerably in recent years. However, fish are ideal hosts of numerous parasites, highlighting the need to develop new research methodologies for its detection. The aim of present study was to compare the efficacy of Ascocotyle metacercariae (Trematoda: Digenea) extraction from visceral tissues of Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) by two methods: homogenization by blender or mixer. Twenty-six samples of M. liza were collected, being 16 liver samples and 10 samples of muscle tissue. Approximately 5g of each sample were processed by blender and mixer techniques homogenization for metacercariae extraction. In liver samples, up to 46 metacercariae were found in samples homogenized in blender. The lowest amount found was 2 metacercariae for blender and mixer techniques. In samples of muscle tissue, 4 metacercariae were observed in the mixer extraction. The lowest amount was found to be 2 parasites to blender and mixer. The mean metacercariae found and extracted from muscle tissue were 0.2 (+0.357) and 1.2 (+0.963) for blender and mixer, respectively. The averages of metacercariae found and extracted from fish liver, in blender and mixer, were 24 (+15.145) and 18 (+8.246), respectively. The homogenization techniques for blender and mixer were effective for the extraction of metacercariae of mullet fish tissues, suggesting that they may be directly applicable in the field of study, especially due to the ease of testing.KEYWORDS: Epidemiology, health management, homogenization, Metacercariae, Mugil liza. |
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COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)COMPARAÇÃO ENTRE OS MÉTODOS DE EXTRAÇÃO DE METACERCÁRIAS DE Ascocotyle sp (Trematoda: Digenea) DOS TECIDOS DE Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)AquaculturePisciculturaThe demand and consumption of fish and their derivatives has increased considerably in recent years. However, fish are ideal hosts of numerous parasites, highlighting the need to develop new research methodologies for its detection. The aim of present study was to compare the efficacy of Ascocotyle metacercariae (Trematoda: Digenea) extraction from visceral tissues of Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) by two methods: homogenization by blender or mixer. Twenty-six samples of M. liza were collected, being 16 liver samples and 10 samples of muscle tissue. Approximately 5g of each sample were processed by blender and mixer techniques homogenization for metacercariae extraction. In liver samples, up to 46 metacercariae were found in samples homogenized in blender. The lowest amount found was 2 metacercariae for blender and mixer techniques. In samples of muscle tissue, 4 metacercariae were observed in the mixer extraction. The lowest amount was found to be 2 parasites to blender and mixer. The mean metacercariae found and extracted from muscle tissue were 0.2 (+0.357) and 1.2 (+0.963) for blender and mixer, respectively. The averages of metacercariae found and extracted from fish liver, in blender and mixer, were 24 (+15.145) and 18 (+8.246), respectively. The homogenization techniques for blender and mixer were effective for the extraction of metacercariae of mullet fish tissues, suggesting that they may be directly applicable in the field of study, especially due to the ease of testing.KEYWORDS: Epidemiology, health management, homogenization, Metacercariae, Mugil liza. Nos últimos anos notou-se considerável aumento na procura e consumo de pescado, além de seus derivados. Contudo, os peixes são hospedeiros ideais de inúmeros parasitas, evidenciando a necessidade de desenvolvimento de novas metodologias de pesquisa para sua detecção. O objetivo do presente trabalho foi comparar a eficácia da extração de metacercárias de Ascocotyle (Trematoda: Digenea) em tecidos viscerais de Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae), mediante duas metodologias: homogeneização por liquidificador ou por mixer. Foram coletadas 26 amostras, sendo 16 amostras de fígado e 10 amostras de tecido muscular de espécimes de M. liza. Cerca de 5g de cada amostra foram processadas pela técnica de homogeneização por liquidificador e pela técnica de homogeneização por mixer para a extração de metacercárias. Em amostras de fígado, até 46 metacercárias foram encontradas na homogeneização em liquidificador. A menor quantidade encontrada foi de 2 metacercárias, para liquidificador e mixer. Em amostras de tecido muscular, observaram-se até 4 metacercárias na extração por mixer. A menor quantidade encontrada em amostras positivas foi de 1 parasita, para liquidificador e mixer. As médias de metacercárias encontradas e extraídas, por liquidificador e mixer, de tecido muscular foram de 0,2 (+0,357) e 1,2 (+0,963), respectivamente. Já as médias de metacercárias encontradas e extraídas, por liquidificador e mixer, do fígado dos peixes foram de 24 (+15,145) e 18 (+8,246), respectivamente. As técnicas de homogeneização, por liquidificador e mixer mostraram-se eficazes para extração de metacercárias dos tecidos de peixes mugilídeos, sugerindo que podem se aplicáveis diretamente no campo de estudo, especialmente pela facilidade de realização do ensaio.PALAVRAS-CHAVE: epidemiologia, gestão em saúde, homogeneização, metacercária, Mugil liza.Universidade Federal de Goiás2014-09-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2816310.5216/cab.v15i3.28163Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 3 (2014); 354-361Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 3 (2014); 354-3611809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/28163/17090https://revistas.ufg.br/vet/article/view/28163/17091Ferraz, Renato Ribeiro NogueiraNamba, Thales KodiNigro, Carolina AlencarRodrigues, Francisco Sandro MenezesBarnabé, Anderson SenaFornari, João Victorinfo:eu-repo/semantics/openAccess2015-03-05T13:08:34Zoai:ojs.revistas.ufg.br:article/28163Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:05.110761Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) COMPARAÇÃO ENTRE OS MÉTODOS DE EXTRAÇÃO DE METACERCÁRIAS DE Ascocotyle sp (Trematoda: Digenea) DOS TECIDOS DE Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) |
title |
COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) |
spellingShingle |
COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) Ferraz, Renato Ribeiro Nogueira Aquaculture Piscicultura |
title_short |
COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) |
title_full |
COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) |
title_fullStr |
COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) |
title_full_unstemmed |
COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) |
title_sort |
COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) |
author |
Ferraz, Renato Ribeiro Nogueira |
author_facet |
Ferraz, Renato Ribeiro Nogueira Namba, Thales Kodi Nigro, Carolina Alencar Rodrigues, Francisco Sandro Menezes Barnabé, Anderson Sena Fornari, João Victor |
author_role |
author |
author2 |
Namba, Thales Kodi Nigro, Carolina Alencar Rodrigues, Francisco Sandro Menezes Barnabé, Anderson Sena Fornari, João Victor |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ferraz, Renato Ribeiro Nogueira Namba, Thales Kodi Nigro, Carolina Alencar Rodrigues, Francisco Sandro Menezes Barnabé, Anderson Sena Fornari, João Victor |
dc.subject.por.fl_str_mv |
Aquaculture Piscicultura |
topic |
Aquaculture Piscicultura |
description |
The demand and consumption of fish and their derivatives has increased considerably in recent years. However, fish are ideal hosts of numerous parasites, highlighting the need to develop new research methodologies for its detection. The aim of present study was to compare the efficacy of Ascocotyle metacercariae (Trematoda: Digenea) extraction from visceral tissues of Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) by two methods: homogenization by blender or mixer. Twenty-six samples of M. liza were collected, being 16 liver samples and 10 samples of muscle tissue. Approximately 5g of each sample were processed by blender and mixer techniques homogenization for metacercariae extraction. In liver samples, up to 46 metacercariae were found in samples homogenized in blender. The lowest amount found was 2 metacercariae for blender and mixer techniques. In samples of muscle tissue, 4 metacercariae were observed in the mixer extraction. The lowest amount was found to be 2 parasites to blender and mixer. The mean metacercariae found and extracted from muscle tissue were 0.2 (+0.357) and 1.2 (+0.963) for blender and mixer, respectively. The averages of metacercariae found and extracted from fish liver, in blender and mixer, were 24 (+15.145) and 18 (+8.246), respectively. The homogenization techniques for blender and mixer were effective for the extraction of metacercariae of mullet fish tissues, suggesting that they may be directly applicable in the field of study, especially due to the ease of testing.KEYWORDS: Epidemiology, health management, homogenization, Metacercariae, Mugil liza. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Avaliado por pares Pesquisa Científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/28163 10.5216/cab.v15i3.28163 |
url |
https://revistas.ufg.br/vet/article/view/28163 |
identifier_str_mv |
10.5216/cab.v15i3.28163 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/28163/17090 https://revistas.ufg.br/vet/article/view/28163/17091 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 3 (2014); 354-361 Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 3 (2014); 354-361 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874788629413888 |