COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)

Detalhes bibliográficos
Autor(a) principal: Ferraz, Renato Ribeiro Nogueira
Data de Publicação: 2014
Outros Autores: Namba, Thales Kodi, Nigro, Carolina Alencar, Rodrigues, Francisco Sandro Menezes, Barnabé, Anderson Sena, Fornari, João Victor
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/28163
Resumo: The demand and consumption of fish and their derivatives has increased considerably in recent years. However, fish are ideal hosts of numerous parasites, highlighting the need to develop new research methodologies for its detection. The aim of present study was to compare the efficacy of Ascocotyle metacercariae (Trematoda: Digenea) extraction from visceral tissues of Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) by two methods: homogenization by blender or mixer. Twenty-six samples of M. liza were collected, being 16 liver samples and 10 samples of muscle tissue. Approximately 5g of each sample were processed by blender and mixer techniques homogenization for metacercariae extraction. In liver samples, up to 46 metacercariae were found in samples homogenized in blender. The lowest amount found was 2 metacercariae for blender and mixer techniques. In samples of muscle tissue, 4 metacercariae were observed in the mixer extraction. The lowest amount was found to be 2 parasites to blender and mixer. The mean metacercariae found and extracted from muscle tissue were 0.2 (+0.357) and 1.2 (+0.963) for blender and mixer, respectively. The averages of metacercariae found and extracted from fish liver, in blender and mixer, were 24 (+15.145) and 18 (+8.246), respectively. The homogenization techniques for blender and mixer were effective for the extraction of metacercariae of mullet fish tissues, suggesting that they may be directly applicable in the field of study, especially due to the ease of testing.KEYWORDS: Epidemiology, health management, homogenization, Metacercariae, Mugil liza.  
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spelling COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)COMPARAÇÃO ENTRE OS MÉTODOS DE EXTRAÇÃO DE METACERCÁRIAS DE Ascocotyle sp (Trematoda: Digenea) DOS TECIDOS DE Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)AquaculturePisciculturaThe demand and consumption of fish and their derivatives has increased considerably in recent years. However, fish are ideal hosts of numerous parasites, highlighting the need to develop new research methodologies for its detection. The aim of present study was to compare the efficacy of Ascocotyle metacercariae (Trematoda: Digenea) extraction from visceral tissues of Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) by two methods: homogenization by blender or mixer. Twenty-six samples of M. liza were collected, being 16 liver samples and 10 samples of muscle tissue. Approximately 5g of each sample were processed by blender and mixer techniques homogenization for metacercariae extraction. In liver samples, up to 46 metacercariae were found in samples homogenized in blender. The lowest amount found was 2 metacercariae for blender and mixer techniques. In samples of muscle tissue, 4 metacercariae were observed in the mixer extraction. The lowest amount was found to be 2 parasites to blender and mixer. The mean metacercariae found and extracted from muscle tissue were 0.2 (+0.357) and 1.2 (+0.963) for blender and mixer, respectively. The averages of metacercariae found and extracted from fish liver, in blender and mixer, were 24 (+15.145) and 18 (+8.246), respectively. The homogenization techniques for blender and mixer were effective for the extraction of metacercariae of mullet fish tissues, suggesting that they may be directly applicable in the field of study, especially due to the ease of testing.KEYWORDS: Epidemiology, health management, homogenization, Metacercariae, Mugil liza.  Nos últimos anos notou-se considerável aumento na procura e consumo de pescado, além de seus derivados. Contudo, os peixes são hospedeiros ideais de inúmeros parasitas, evidenciando a necessidade de desenvolvimento de novas metodologias de pesquisa para sua detecção. O objetivo do presente trabalho foi comparar a eficácia da extração de metacercárias de Ascocotyle (Trematoda: Digenea) em tecidos viscerais de Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae), mediante duas metodologias: homogeneização por liquidificador ou por mixer. Foram coletadas 26 amostras, sendo 16 amostras de fígado e 10 amostras de tecido muscular de espécimes de M. liza. Cerca de 5g de cada amostra foram processadas pela técnica de homogeneização por liquidificador e pela técnica de homogeneização por mixer para a extração de metacercárias. Em amostras de fígado, até 46 metacercárias foram encontradas na homogeneização em liquidificador. A menor quantidade encontrada foi de 2 metacercárias, para liquidificador e mixer. Em amostras de tecido muscular, observaram-se até 4 metacercárias na extração por mixer. A menor quantidade encontrada em amostras positivas foi de 1 parasita, para liquidificador e mixer. As médias de metacercárias encontradas e extraídas, por liquidificador e mixer, de tecido muscular foram de 0,2 (+0,357) e 1,2 (+0,963), respectivamente. Já as médias de metacercárias encontradas e extraídas, por liquidificador e mixer, do fígado dos peixes foram de 24 (+15,145) e 18 (+8,246), respectivamente. As técnicas de homogeneização, por liquidificador e mixer mostraram-se eficazes para extração de metacercárias dos tecidos de peixes mugilídeos, sugerindo que podem se aplicáveis diretamente no campo de estudo, especialmente pela facilidade de realização do ensaio.PALAVRAS-CHAVE: epidemiologia, gestão em saúde, homogeneização, metacercária, Mugil liza.Universidade Federal de Goiás2014-09-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2816310.5216/cab.v15i3.28163Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 3 (2014); 354-361Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 3 (2014); 354-3611809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/28163/17090https://revistas.ufg.br/vet/article/view/28163/17091Ferraz, Renato Ribeiro NogueiraNamba, Thales KodiNigro, Carolina AlencarRodrigues, Francisco Sandro MenezesBarnabé, Anderson SenaFornari, João Victorinfo:eu-repo/semantics/openAccess2015-03-05T13:08:34Zoai:ojs.revistas.ufg.br:article/28163Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:05.110761Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)
COMPARAÇÃO ENTRE OS MÉTODOS DE EXTRAÇÃO DE METACERCÁRIAS DE Ascocotyle sp (Trematoda: Digenea) DOS TECIDOS DE Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)
title COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)
spellingShingle COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)
Ferraz, Renato Ribeiro Nogueira
Aquaculture
Piscicultura
title_short COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)
title_full COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)
title_fullStr COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)
title_full_unstemmed COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)
title_sort COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)
author Ferraz, Renato Ribeiro Nogueira
author_facet Ferraz, Renato Ribeiro Nogueira
Namba, Thales Kodi
Nigro, Carolina Alencar
Rodrigues, Francisco Sandro Menezes
Barnabé, Anderson Sena
Fornari, João Victor
author_role author
author2 Namba, Thales Kodi
Nigro, Carolina Alencar
Rodrigues, Francisco Sandro Menezes
Barnabé, Anderson Sena
Fornari, João Victor
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ferraz, Renato Ribeiro Nogueira
Namba, Thales Kodi
Nigro, Carolina Alencar
Rodrigues, Francisco Sandro Menezes
Barnabé, Anderson Sena
Fornari, João Victor
dc.subject.por.fl_str_mv Aquaculture
Piscicultura
topic Aquaculture
Piscicultura
description The demand and consumption of fish and their derivatives has increased considerably in recent years. However, fish are ideal hosts of numerous parasites, highlighting the need to develop new research methodologies for its detection. The aim of present study was to compare the efficacy of Ascocotyle metacercariae (Trematoda: Digenea) extraction from visceral tissues of Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) by two methods: homogenization by blender or mixer. Twenty-six samples of M. liza were collected, being 16 liver samples and 10 samples of muscle tissue. Approximately 5g of each sample were processed by blender and mixer techniques homogenization for metacercariae extraction. In liver samples, up to 46 metacercariae were found in samples homogenized in blender. The lowest amount found was 2 metacercariae for blender and mixer techniques. In samples of muscle tissue, 4 metacercariae were observed in the mixer extraction. The lowest amount was found to be 2 parasites to blender and mixer. The mean metacercariae found and extracted from muscle tissue were 0.2 (+0.357) and 1.2 (+0.963) for blender and mixer, respectively. The averages of metacercariae found and extracted from fish liver, in blender and mixer, were 24 (+15.145) and 18 (+8.246), respectively. The homogenization techniques for blender and mixer were effective for the extraction of metacercariae of mullet fish tissues, suggesting that they may be directly applicable in the field of study, especially due to the ease of testing.KEYWORDS: Epidemiology, health management, homogenization, Metacercariae, Mugil liza.  
publishDate 2014
dc.date.none.fl_str_mv 2014-09-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/28163
10.5216/cab.v15i3.28163
url https://revistas.ufg.br/vet/article/view/28163
identifier_str_mv 10.5216/cab.v15i3.28163
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/28163/17090
https://revistas.ufg.br/vet/article/view/28163/17091
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 3 (2014); 354-361
Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 3 (2014); 354-361
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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