CRYOPRESERVATION CANINE SEMEN USING A POWDERED COCONUT WATER EXTENDER (APC-106): EFFECT OF GLYCEROL TEMPERATURE ADDITION (27OC AND 4OC)

• Autor(a) principal: Barbosa, Claudia da Cunha
• Data de Publicação: 2009
• Outros Autores: Madeira, Victor Leão Hitzschky, Jucá, Ricardo Parente, Oliveira, Ângela Cristina de, Uchoa, Daniel Couto, Silva, Lúcia Daniel Machado da
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Ciência animal brasileira (Online)
• Texto Completo: https://revistas.ufg.br/vet/article/view/2356
• topic: Animal production; Reprodução Animal

The aim of the present study was to compare the effect of glycerol addition at two temperatures on canine semen extended and frozen with powdered coconut water extender (ACP-106®). Twelve ejaculates were collected, the sperm-rich fraction was evaluated and further divided into two aliquots. The first aliquot was extended in ACP-106® with 5% egg yolk and glycerol at 27ºC (G27) and the second one was also extended in ACP-106® with 5% egg yolk, with glycerol addition at 4ºC (G4). Samples were frozen, thawed and submitted to evaluations of morphology, acrossomal integrity, hypo-osmotic swelling and alive spermatozoa. No differences were observed between groups after thawing regarding all seminal parameters. In computerized analyzes, it was also not evidenced differences between groups for total and progressive motility, percentual of spermatozoa with fast and medium velocity, and average path velocity (VAP) of spermatozoa with fast and medium velocity, being observed in G4 and G27, respectively, a total motility of 24.45 ± 3.87% and 31.65 ± 3.93% with the VAP of the fast spermatozoa of 91.24 ± 7.74µm/s and 106.25 ± 3.94µm/s. In conclusion, the glycerol temperature addition does not influence the quality of post-thawed canine semen diluted in ACP-106®.KEY WORDS: ACP-106®, cryopreservation, dog, glycerol, semen.