Use of galactose to vitrify ram semen in straws

Detalhes bibliográficos
Autor(a) principal: Calixto Matias, Márcio
Data de Publicação: 2021
Outros Autores: Rodolf Ribeiro Cézar, Allan, Carla Cavalcanti Marques, Juliana, Karla Ataide da Silva, Fernanda, Nayreli Freire Gonçalves Sandes, Vitória, Câmara, Diogo Ribeiro
Tipo de documento: Artigo
Idioma: por
eng
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/67525
Resumo: Sperm vitrification is a technique with great potential for cryopreservation of genetic material, with superior effectiveness compared to conventional methods in some species. However, few studies have shown its efficiency with ejaculated sperm of rams and the use of galactose as an extracellular cryoprotectant during vitrification. This study aimed to evaluate the effect of galactose (0.01 M), with or without glycerol addition (3% and 7%) in a commercial extender (Steridyl® - control) for ram sperm cryopreservation in straws, comparing the classic freezing method and vitrification. Ejaculates from six breeding soundness Dorper rams were collected with an artificial vagina, aliquoted, individually diluted (100 × 106 sperm/mL) on extenders tested, loaded into 0.25 mL straws, and subjected to a classic freezing method or vitrification. Sperm kinematics, morphology, morphometry, viability, and physical and functional integrity of the sperm membrane were evaluated. The classic freezing method resulted in higher total and progressive motility than vitrification, as no motility was detected in vitrified samples after rewarming (p<0.05). The addition of galactose or glycerol to the commercial medium did not benefit both vitrification and the classic freezing method.Keywords: Semen biotechnology; Ultra-rapid freezing; Cryoprotectants; Reproduction.
id UFG-7_cfd7435a4b6d849a2413c49e30f795b6
oai_identifier_str oai:ojs.revistas.ufg.br:article/67525
network_acronym_str UFG-7
network_name_str Ciência animal brasileira (Online)
repository_id_str
spelling Use of galactose to vitrify ram semen in strawsUso da galactose na vitrificação de sêmen ovino em palhetasSperm vitrification is a technique with great potential for cryopreservation of genetic material, with superior effectiveness compared to conventional methods in some species. However, few studies have shown its efficiency with ejaculated sperm of rams and the use of galactose as an extracellular cryoprotectant during vitrification. This study aimed to evaluate the effect of galactose (0.01 M), with or without glycerol addition (3% and 7%) in a commercial extender (Steridyl® - control) for ram sperm cryopreservation in straws, comparing the classic freezing method and vitrification. Ejaculates from six breeding soundness Dorper rams were collected with an artificial vagina, aliquoted, individually diluted (100 × 106 sperm/mL) on extenders tested, loaded into 0.25 mL straws, and subjected to a classic freezing method or vitrification. Sperm kinematics, morphology, morphometry, viability, and physical and functional integrity of the sperm membrane were evaluated. The classic freezing method resulted in higher total and progressive motility than vitrification, as no motility was detected in vitrified samples after rewarming (p<0.05). The addition of galactose or glycerol to the commercial medium did not benefit both vitrification and the classic freezing method.Keywords: Semen biotechnology; Ultra-rapid freezing; Cryoprotectants; Reproduction.A vitrificação de espermatozoides é uma técnica que apresenta grande potencial para criopreservação de material genético, e sua eficácia tem sido superior aos métodos convencionais em algumas espécies. No entanto, existem poucos estudos sobre sua eficiência com sêmen ejaculado de carneiros e o uso da galactose como crioprotetor extracelular durante a vitrificação. Objetivou-se com este estudo avaliar o efeito da galactose (0,01 M), associada ou não ao glicerol (3% e 7%), em meio comercial (Steridyl® - controle), na criopreservação de espermatozoides de carneiros pelo método de palhetas, comparando o método clássico de congelação e a vitrificação. Ejaculados de seis carneiros da raça Dorper em idade reprodutiva foram coletados com vagina artificial, aliquotados, diluídos individualmente (100 × 106 espermatozoides/mL) nos meios testados, envasados em palhetas de 0,25 mL e submetidos à congelação clássica ou vitrificação. Foram analisadas a cinemática, morfologia, morfometria, viabilidade, integridade física e funcional da membrana espermática. A congelação clássica obteve melhores resultados de motilidade total e progressiva do que a vitrificação nos quatro extensores testados, uma vez que as amostras vitrificadas não apresentaram motilidade pós-reaquecimento (p < 0,05). A adição de galactose ou glicerol ao meio comercial não trouxe efeito benéfico tanto para a vitrificação quanto congelação clássica.Palavras-chave: Biotecnologia de sêmen; Congelamento ultrarrápido; Crioprotetores; Reprodução.Universidade Federal de Goiás2021-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://revistas.ufg.br/vet/article/view/67525Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 22 (2021): Continuous publicationCiência Animal Brasileira / Brazilian Animal Science; v. 22 (2021): Publicação contínua1809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporenghttps://revistas.ufg.br/vet/article/view/67525/36742https://revistas.ufg.br/vet/article/view/67525/36939https://revistas.ufg.br/vet/article/view/67525/36744https://revistas.ufg.br/vet/article/view/67525/36940Copyright (c) 2021 Ciência Animal Brasileirahttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCalixto Matias, MárcioRodolf Ribeiro Cézar, AllanCarla Cavalcanti Marques, JulianaKarla Ataide da Silva, FernandaNayreli Freire Gonçalves Sandes, VitóriaCâmara, Diogo Ribeiro2023-03-23T18:53:00Zoai:ojs.revistas.ufg.br:article/67525Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:27.152456Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv Use of galactose to vitrify ram semen in straws
Uso da galactose na vitrificação de sêmen ovino em palhetas
title Use of galactose to vitrify ram semen in straws
spellingShingle Use of galactose to vitrify ram semen in straws
Calixto Matias, Márcio
title_short Use of galactose to vitrify ram semen in straws
title_full Use of galactose to vitrify ram semen in straws
title_fullStr Use of galactose to vitrify ram semen in straws
title_full_unstemmed Use of galactose to vitrify ram semen in straws
title_sort Use of galactose to vitrify ram semen in straws
author Calixto Matias, Márcio
author_facet Calixto Matias, Márcio
Rodolf Ribeiro Cézar, Allan
Carla Cavalcanti Marques, Juliana
Karla Ataide da Silva, Fernanda
Nayreli Freire Gonçalves Sandes, Vitória
Câmara, Diogo Ribeiro
author_role author
author2 Rodolf Ribeiro Cézar, Allan
Carla Cavalcanti Marques, Juliana
Karla Ataide da Silva, Fernanda
Nayreli Freire Gonçalves Sandes, Vitória
Câmara, Diogo Ribeiro
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Calixto Matias, Márcio
Rodolf Ribeiro Cézar, Allan
Carla Cavalcanti Marques, Juliana
Karla Ataide da Silva, Fernanda
Nayreli Freire Gonçalves Sandes, Vitória
Câmara, Diogo Ribeiro
description Sperm vitrification is a technique with great potential for cryopreservation of genetic material, with superior effectiveness compared to conventional methods in some species. However, few studies have shown its efficiency with ejaculated sperm of rams and the use of galactose as an extracellular cryoprotectant during vitrification. This study aimed to evaluate the effect of galactose (0.01 M), with or without glycerol addition (3% and 7%) in a commercial extender (Steridyl® - control) for ram sperm cryopreservation in straws, comparing the classic freezing method and vitrification. Ejaculates from six breeding soundness Dorper rams were collected with an artificial vagina, aliquoted, individually diluted (100 × 106 sperm/mL) on extenders tested, loaded into 0.25 mL straws, and subjected to a classic freezing method or vitrification. Sperm kinematics, morphology, morphometry, viability, and physical and functional integrity of the sperm membrane were evaluated. The classic freezing method resulted in higher total and progressive motility than vitrification, as no motility was detected in vitrified samples after rewarming (p<0.05). The addition of galactose or glycerol to the commercial medium did not benefit both vitrification and the classic freezing method.Keywords: Semen biotechnology; Ultra-rapid freezing; Cryoprotectants; Reproduction.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/67525
url https://revistas.ufg.br/vet/article/view/67525
dc.language.iso.fl_str_mv por
eng
language por
eng
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/67525/36742
https://revistas.ufg.br/vet/article/view/67525/36939
https://revistas.ufg.br/vet/article/view/67525/36744
https://revistas.ufg.br/vet/article/view/67525/36940
dc.rights.driver.fl_str_mv Copyright (c) 2021 Ciência Animal Brasileira
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Ciência Animal Brasileira
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 22 (2021): Continuous publication
Ciência Animal Brasileira / Brazilian Animal Science; v. 22 (2021): Publicação contínua
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
_version_ 1799874790505316352