Use of galactose to vitrify ram semen in straws
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/67525 |
Resumo: | Sperm vitrification is a technique with great potential for cryopreservation of genetic material, with superior effectiveness compared to conventional methods in some species. However, few studies have shown its efficiency with ejaculated sperm of rams and the use of galactose as an extracellular cryoprotectant during vitrification. This study aimed to evaluate the effect of galactose (0.01 M), with or without glycerol addition (3% and 7%) in a commercial extender (Steridyl® - control) for ram sperm cryopreservation in straws, comparing the classic freezing method and vitrification. Ejaculates from six breeding soundness Dorper rams were collected with an artificial vagina, aliquoted, individually diluted (100 × 106 sperm/mL) on extenders tested, loaded into 0.25 mL straws, and subjected to a classic freezing method or vitrification. Sperm kinematics, morphology, morphometry, viability, and physical and functional integrity of the sperm membrane were evaluated. The classic freezing method resulted in higher total and progressive motility than vitrification, as no motility was detected in vitrified samples after rewarming (p<0.05). The addition of galactose or glycerol to the commercial medium did not benefit both vitrification and the classic freezing method.Keywords: Semen biotechnology; Ultra-rapid freezing; Cryoprotectants; Reproduction. |
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Use of galactose to vitrify ram semen in strawsUso da galactose na vitrificação de sêmen ovino em palhetasSperm vitrification is a technique with great potential for cryopreservation of genetic material, with superior effectiveness compared to conventional methods in some species. However, few studies have shown its efficiency with ejaculated sperm of rams and the use of galactose as an extracellular cryoprotectant during vitrification. This study aimed to evaluate the effect of galactose (0.01 M), with or without glycerol addition (3% and 7%) in a commercial extender (Steridyl® - control) for ram sperm cryopreservation in straws, comparing the classic freezing method and vitrification. Ejaculates from six breeding soundness Dorper rams were collected with an artificial vagina, aliquoted, individually diluted (100 × 106 sperm/mL) on extenders tested, loaded into 0.25 mL straws, and subjected to a classic freezing method or vitrification. Sperm kinematics, morphology, morphometry, viability, and physical and functional integrity of the sperm membrane were evaluated. The classic freezing method resulted in higher total and progressive motility than vitrification, as no motility was detected in vitrified samples after rewarming (p<0.05). The addition of galactose or glycerol to the commercial medium did not benefit both vitrification and the classic freezing method.Keywords: Semen biotechnology; Ultra-rapid freezing; Cryoprotectants; Reproduction.A vitrificação de espermatozoides é uma técnica que apresenta grande potencial para criopreservação de material genético, e sua eficácia tem sido superior aos métodos convencionais em algumas espécies. No entanto, existem poucos estudos sobre sua eficiência com sêmen ejaculado de carneiros e o uso da galactose como crioprotetor extracelular durante a vitrificação. Objetivou-se com este estudo avaliar o efeito da galactose (0,01 M), associada ou não ao glicerol (3% e 7%), em meio comercial (Steridyl® - controle), na criopreservação de espermatozoides de carneiros pelo método de palhetas, comparando o método clássico de congelação e a vitrificação. Ejaculados de seis carneiros da raça Dorper em idade reprodutiva foram coletados com vagina artificial, aliquotados, diluídos individualmente (100 × 106 espermatozoides/mL) nos meios testados, envasados em palhetas de 0,25 mL e submetidos à congelação clássica ou vitrificação. Foram analisadas a cinemática, morfologia, morfometria, viabilidade, integridade física e funcional da membrana espermática. A congelação clássica obteve melhores resultados de motilidade total e progressiva do que a vitrificação nos quatro extensores testados, uma vez que as amostras vitrificadas não apresentaram motilidade pós-reaquecimento (p < 0,05). A adição de galactose ou glicerol ao meio comercial não trouxe efeito benéfico tanto para a vitrificação quanto congelação clássica.Palavras-chave: Biotecnologia de sêmen; Congelamento ultrarrápido; Crioprotetores; Reprodução.Universidade Federal de Goiás2021-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://revistas.ufg.br/vet/article/view/67525Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 22 (2021): Continuous publicationCiência Animal Brasileira / Brazilian Animal Science; v. 22 (2021): Publicação contínua1809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporenghttps://revistas.ufg.br/vet/article/view/67525/36742https://revistas.ufg.br/vet/article/view/67525/36939https://revistas.ufg.br/vet/article/view/67525/36744https://revistas.ufg.br/vet/article/view/67525/36940Copyright (c) 2021 Ciência Animal Brasileirahttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCalixto Matias, MárcioRodolf Ribeiro Cézar, AllanCarla Cavalcanti Marques, JulianaKarla Ataide da Silva, FernandaNayreli Freire Gonçalves Sandes, VitóriaCâmara, Diogo Ribeiro2023-03-23T18:53:00Zoai:ojs.revistas.ufg.br:article/67525Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:27.152456Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
Use of galactose to vitrify ram semen in straws Uso da galactose na vitrificação de sêmen ovino em palhetas |
title |
Use of galactose to vitrify ram semen in straws |
spellingShingle |
Use of galactose to vitrify ram semen in straws Calixto Matias, Márcio |
title_short |
Use of galactose to vitrify ram semen in straws |
title_full |
Use of galactose to vitrify ram semen in straws |
title_fullStr |
Use of galactose to vitrify ram semen in straws |
title_full_unstemmed |
Use of galactose to vitrify ram semen in straws |
title_sort |
Use of galactose to vitrify ram semen in straws |
author |
Calixto Matias, Márcio |
author_facet |
Calixto Matias, Márcio Rodolf Ribeiro Cézar, Allan Carla Cavalcanti Marques, Juliana Karla Ataide da Silva, Fernanda Nayreli Freire Gonçalves Sandes, Vitória Câmara, Diogo Ribeiro |
author_role |
author |
author2 |
Rodolf Ribeiro Cézar, Allan Carla Cavalcanti Marques, Juliana Karla Ataide da Silva, Fernanda Nayreli Freire Gonçalves Sandes, Vitória Câmara, Diogo Ribeiro |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Calixto Matias, Márcio Rodolf Ribeiro Cézar, Allan Carla Cavalcanti Marques, Juliana Karla Ataide da Silva, Fernanda Nayreli Freire Gonçalves Sandes, Vitória Câmara, Diogo Ribeiro |
description |
Sperm vitrification is a technique with great potential for cryopreservation of genetic material, with superior effectiveness compared to conventional methods in some species. However, few studies have shown its efficiency with ejaculated sperm of rams and the use of galactose as an extracellular cryoprotectant during vitrification. This study aimed to evaluate the effect of galactose (0.01 M), with or without glycerol addition (3% and 7%) in a commercial extender (Steridyl® - control) for ram sperm cryopreservation in straws, comparing the classic freezing method and vitrification. Ejaculates from six breeding soundness Dorper rams were collected with an artificial vagina, aliquoted, individually diluted (100 × 106 sperm/mL) on extenders tested, loaded into 0.25 mL straws, and subjected to a classic freezing method or vitrification. Sperm kinematics, morphology, morphometry, viability, and physical and functional integrity of the sperm membrane were evaluated. The classic freezing method resulted in higher total and progressive motility than vitrification, as no motility was detected in vitrified samples after rewarming (p<0.05). The addition of galactose or glycerol to the commercial medium did not benefit both vitrification and the classic freezing method.Keywords: Semen biotechnology; Ultra-rapid freezing; Cryoprotectants; Reproduction. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/67525 |
url |
https://revistas.ufg.br/vet/article/view/67525 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/67525/36742 https://revistas.ufg.br/vet/article/view/67525/36939 https://revistas.ufg.br/vet/article/view/67525/36744 https://revistas.ufg.br/vet/article/view/67525/36940 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Ciência Animal Brasileira http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Ciência Animal Brasileira http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 22 (2021): Continuous publication Ciência Animal Brasileira / Brazilian Animal Science; v. 22 (2021): Publicação contínua 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874790505316352 |