PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC

Detalhes bibliográficos
Autor(a) principal: Devens, Bruna Alves
Data de Publicação: 2014
Outros Autores: Vargas Viloria, Marlene Isabel, Souza e Silva, Carlos Henryque de, Salcedo, Joaquin Hernán Patarroyo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
DOI: 10.5216/cab.v15i2.23017
Texto Completo: https://revistas.ufg.br/vet/article/view/23017
Resumo: The Neospora caninum is a protozoan Apicomplexa with greater involvement in abortions worldwide. The economic losses determined by neosporosis also include abortions besides the early disposal of cows, costs for replacing animals in the herd, drop in milk production as well as milk in fat production. The immunological diagnosis involves purchasing costly diagnostic kits on the market. Therefore, the aim of this study was the production of hybridomas secreting polyclonal antibodies with affinity to Neospora caninum (Nc-1 strain) for immunodiagnostic use. For antibodies production, we used sonicated protozoa from Vero cells in culture, purified by filtration. These tachyzoites were employed for immunization of BALB / c mice using saponin as adjuvant, which allowed obtaining polyclonal antibodies capable of revealing fluorescein reaction in indirect immunofluorescence. The fusion of splenic cells, from the immunized mice with myeloma cells SP2 / 0 resulted in 72.4% hybridomas secreting anti-Nc-1antibodies. These hybridomas secreted antibodies positive to N. caninum and negative to Toxoplasma gondii.KEYWORDS: hybridomas, immunodiagnosis, monoclonal antibodies, neosporosis.
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spelling PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTICPRODUÇÃO DE HIBRIDOMAS SECRETORES DE ANTICORPOS ANTI- Neospora caninum PARA USO EM IMUNODIAGNÓSTICOAnimal sanitySanidade AnimalThe Neospora caninum is a protozoan Apicomplexa with greater involvement in abortions worldwide. The economic losses determined by neosporosis also include abortions besides the early disposal of cows, costs for replacing animals in the herd, drop in milk production as well as milk in fat production. The immunological diagnosis involves purchasing costly diagnostic kits on the market. Therefore, the aim of this study was the production of hybridomas secreting polyclonal antibodies with affinity to Neospora caninum (Nc-1 strain) for immunodiagnostic use. For antibodies production, we used sonicated protozoa from Vero cells in culture, purified by filtration. These tachyzoites were employed for immunization of BALB / c mice using saponin as adjuvant, which allowed obtaining polyclonal antibodies capable of revealing fluorescein reaction in indirect immunofluorescence. The fusion of splenic cells, from the immunized mice with myeloma cells SP2 / 0 resulted in 72.4% hybridomas secreting anti-Nc-1antibodies. These hybridomas secreted antibodies positive to N. caninum and negative to Toxoplasma gondii.KEYWORDS: hybridomas, immunodiagnosis, monoclonal antibodies, neosporosis. O Neospora caninum é um protozoário apicomplexa com maior implicação em abortamentos em muitos países do mundo. As perdas econômicas determinadas pela neosporose incluem além dos abortamentos também o descarte precoce de vacas, os custos de reposição de novos animais no rebanho, a queda na produção leiteira, bem como na produção da gordura no leite. O diagnóstico imunológico envolve a aquisição de kits diagnósticos existentes no mercado os quais apresentam alto custo. Portanto, o objetivo do presente trabalho foi à produção de hibridomas secretores de anticorpos policlonais com afinidade ao Neospora caninum (cepa Nc-1) para utilização em imunodiagnósticos. Para a produção dos anticorpos usou-se o protozoário sonicado, proveniente da cultura em células VERO, que foi purificado por filtração. Esses taquizoítos foram utilizados na imunização dos camundongos BALB/c, usando-se como adjuvante a saponina, o que permitiu a obtenção de anticorpos policlonais capazes de revelar a fluoresceína na reação da imunofluorescência indireta. A fusão das células esplênicas, provenientes dos camundongos imunizados com as células de mieloma SP2/0 resultou na obtenção de 72,4% de hibridomas secretores de anticorpos anti-Nc-1. Esses hibridomas secretaram anticorpos positivos ao N. caninum e negativos ao Toxoplasma gondii.PALAVRAS-CHAVE: anticorpos monoclonais, diagnóstico imunológico, hibridomas, neosporose.   Universidade Federal de Goiás2014-06-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2301710.5216/cab.v15i2.23017Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 2 (2014); 228-233Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 2 (2014); 228-2331809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/23017/16729https://revistas.ufg.br/vet/article/view/23017/16730Devens, Bruna AlvesVargas Viloria, Marlene IsabelSouza e Silva, Carlos Henryque deSalcedo, Joaquin Hernán Patarroyoinfo:eu-repo/semantics/openAccess2014-06-30T13:37:48Zoai:ojs.revistas.ufg.br:article/23017Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:57.924135Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC
PRODUÇÃO DE HIBRIDOMAS SECRETORES DE ANTICORPOS ANTI- Neospora caninum PARA USO EM IMUNODIAGNÓSTICO
title PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC
spellingShingle PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC
PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC
Devens, Bruna Alves
Animal sanity
Sanidade Animal
Devens, Bruna Alves
Animal sanity
Sanidade Animal
title_short PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC
title_full PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC
title_fullStr PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC
PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC
title_full_unstemmed PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC
PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC
title_sort PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC
author Devens, Bruna Alves
author_facet Devens, Bruna Alves
Devens, Bruna Alves
Vargas Viloria, Marlene Isabel
Souza e Silva, Carlos Henryque de
Salcedo, Joaquin Hernán Patarroyo
Vargas Viloria, Marlene Isabel
Souza e Silva, Carlos Henryque de
Salcedo, Joaquin Hernán Patarroyo
author_role author
author2 Vargas Viloria, Marlene Isabel
Souza e Silva, Carlos Henryque de
Salcedo, Joaquin Hernán Patarroyo
author2_role author
author
author
dc.contributor.author.fl_str_mv Devens, Bruna Alves
Vargas Viloria, Marlene Isabel
Souza e Silva, Carlos Henryque de
Salcedo, Joaquin Hernán Patarroyo
dc.subject.por.fl_str_mv Animal sanity
Sanidade Animal
topic Animal sanity
Sanidade Animal
description The Neospora caninum is a protozoan Apicomplexa with greater involvement in abortions worldwide. The economic losses determined by neosporosis also include abortions besides the early disposal of cows, costs for replacing animals in the herd, drop in milk production as well as milk in fat production. The immunological diagnosis involves purchasing costly diagnostic kits on the market. Therefore, the aim of this study was the production of hybridomas secreting polyclonal antibodies with affinity to Neospora caninum (Nc-1 strain) for immunodiagnostic use. For antibodies production, we used sonicated protozoa from Vero cells in culture, purified by filtration. These tachyzoites were employed for immunization of BALB / c mice using saponin as adjuvant, which allowed obtaining polyclonal antibodies capable of revealing fluorescein reaction in indirect immunofluorescence. The fusion of splenic cells, from the immunized mice with myeloma cells SP2 / 0 resulted in 72.4% hybridomas secreting anti-Nc-1antibodies. These hybridomas secreted antibodies positive to N. caninum and negative to Toxoplasma gondii.KEYWORDS: hybridomas, immunodiagnosis, monoclonal antibodies, neosporosis.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/23017
10.5216/cab.v15i2.23017
url https://revistas.ufg.br/vet/article/view/23017
identifier_str_mv 10.5216/cab.v15i2.23017
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/23017/16729
https://revistas.ufg.br/vet/article/view/23017/16730
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 2 (2014); 228-233
Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 2 (2014); 228-233
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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dc.identifier.doi.none.fl_str_mv 10.5216/cab.v15i2.23017

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