IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER

Detalhes bibliográficos
Autor(a) principal: Bispo, Charles André S.
Data de Publicação: 2011
Outros Autores: Pugliesi, Guilherme, Palhão, Miller Pereira, Coelho, Polyana Galvão B., Ker, Pedro Gama, Rodrigues, Marcelo Teixeira, Carvalho, Giovanni Ribeiro
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/13115
Resumo: The objective of this study was to evaluate by in vitro tests and in vivo fertility, two concentrations of egg yolk (T1 = 20% or T2 = 2.5%) in egg yolk-citrate semen extender for storing goat semen at 5°C for 24 hours. Ninety semen collections were performed in four goats. The semen was split and diluted in two different semen extenders and stored in refrigerator at 5°C for 24 hours, until the semen evaluation and insemination procedures. The average values for cooled semen, respectively, for T1 and T2 were motility, 68.5 ± 15.4 and 78.0 ± 5.5 (p < 0.05); vigor, 2.5 ± 0.6 and 3.2 ± 0.3 (p < 0.05), supravital test, 59.4 ± 17.2 and 71.1 ± 10.5 (p < 0.05) and hypoosmotic test, 42.2 ± 15.7 and 56.2 ± 11.5 (p < 0.05). The fertility rate was greater (p < 0.05) in T1 (31.3%) than in T2 (66.7%) and the prolificacy did not differ (p < 0.05) between T1 (1.3 ± 0.5) and T2 (1.4 ± 0.5). In conclusion, the egg yolk-citrate extender with a low concentration (2.5%) of egg yolk was more effective in preserving the viability and fertility of goat semen stored at 5°C for 24 hours.KEYWORDS: artificial insemination; extender; goat; semen; spermatozoa.
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spelling IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDERCARACTERÍSTICAS IN VITRO E FERTILIDADE DO SÊMEN CAPRINO ARMAZENADO A 5°C POR 24 HORAS UTILIZANDO DUAS CONCENTRAÇÕES DE GEMA DE OVO NO DILUENTEAnimal ReproductionReprodução de CaprinosThe objective of this study was to evaluate by in vitro tests and in vivo fertility, two concentrations of egg yolk (T1 = 20% or T2 = 2.5%) in egg yolk-citrate semen extender for storing goat semen at 5°C for 24 hours. Ninety semen collections were performed in four goats. The semen was split and diluted in two different semen extenders and stored in refrigerator at 5°C for 24 hours, until the semen evaluation and insemination procedures. The average values for cooled semen, respectively, for T1 and T2 were motility, 68.5 ± 15.4 and 78.0 ± 5.5 (p < 0.05); vigor, 2.5 ± 0.6 and 3.2 ± 0.3 (p < 0.05), supravital test, 59.4 ± 17.2 and 71.1 ± 10.5 (p < 0.05) and hypoosmotic test, 42.2 ± 15.7 and 56.2 ± 11.5 (p < 0.05). The fertility rate was greater (p < 0.05) in T1 (31.3%) than in T2 (66.7%) and the prolificacy did not differ (p < 0.05) between T1 (1.3 ± 0.5) and T2 (1.4 ± 0.5). In conclusion, the egg yolk-citrate extender with a low concentration (2.5%) of egg yolk was more effective in preserving the viability and fertility of goat semen stored at 5°C for 24 hours.KEYWORDS: artificial insemination; extender; goat; semen; spermatozoa.Objetivou-se com este trabalho avaliar, por meio de testes in vitro e a fertilidade in vivo, duas concentrações de gema de ovo (20% e 2,5%) em diluente citrato-gema de ovo na preservação do sêmen caprino armazenado a 5°C, por 24 horas. Foram realizadas 90 coletas de sêmen de quatro bodes. O sêmen foi diluído em diluente citrato-gema de ovo com duas concentrações de gema de ovo (T1 = 20% ou T2 = 2,5%) e armazenados em geladeira a 5°C, por 24 horas, até a realização das avaliações e inseminações. Os valores médios referentes ao sêmen resfriado foram, respectivamente, para T1 e T2: motilidade total, 68,5 ± 15,4 e 78,0 ± 5,5 (p < 0,05); vigor, 2,5 ± 0,6 e 3,2 ± 0,3 (p < 0,05); supravital, 59,4 ± 17,2 e 71,1 ± 10,5 (p < 0,05) e hiposmótico, 42,2 ± 15,7 e 56,2 ± 11,5 (p < 0,05). As taxa de fertilidade obtida no T1 (31,3%) foi maior em relação ao T2 (66,7%) e a prolificidade não diferiu (p < 0,05) entre o T1 (1,3 ± 0,5) e o T2 (1,4 ± 0,5). Conclui-se que o diluente de citrato-gema de ovo com baixa concentração de gema (2,5%) foi mais eficaz em preservar a viabilidade e a fertilidade do sêmen caprino armazenado a 5°C, por 24 horas.PALAVRAS-CHAVE: bode; diluente; espermatozoide; inseminação artificial; sêmen.Universidade Federal de Goiás2011-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionresearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/1311510.5216/cab.v12i4.13115Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 12 No. 4 (2011); 653-660Ciência Animal Brasileira / Brazilian Animal Science; v. 12 n. 4 (2011); 653-6601809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/13115/10036https://revistas.ufg.br/vet/article/view/13115/10037Bispo, Charles André S.Pugliesi, GuilhermePalhão, Miller PereiraCoelho, Polyana Galvão B.Ker, Pedro GamaRodrigues, Marcelo TeixeiraCarvalho, Giovanni Ribeiroinfo:eu-repo/semantics/openAccess2012-01-30T12:21:41Zoai:ojs.revistas.ufg.br:article/13115Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:46.512866Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER
CARACTERÍSTICAS IN VITRO E FERTILIDADE DO SÊMEN CAPRINO ARMAZENADO A 5°C POR 24 HORAS UTILIZANDO DUAS CONCENTRAÇÕES DE GEMA DE OVO NO DILUENTE
title IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER
spellingShingle IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER
Bispo, Charles André S.
Animal Reproduction
Reprodução de Caprinos
title_short IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER
title_full IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER
title_fullStr IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER
title_full_unstemmed IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER
title_sort IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER
author Bispo, Charles André S.
author_facet Bispo, Charles André S.
Pugliesi, Guilherme
Palhão, Miller Pereira
Coelho, Polyana Galvão B.
Ker, Pedro Gama
Rodrigues, Marcelo Teixeira
Carvalho, Giovanni Ribeiro
author_role author
author2 Pugliesi, Guilherme
Palhão, Miller Pereira
Coelho, Polyana Galvão B.
Ker, Pedro Gama
Rodrigues, Marcelo Teixeira
Carvalho, Giovanni Ribeiro
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bispo, Charles André S.
Pugliesi, Guilherme
Palhão, Miller Pereira
Coelho, Polyana Galvão B.
Ker, Pedro Gama
Rodrigues, Marcelo Teixeira
Carvalho, Giovanni Ribeiro
dc.subject.por.fl_str_mv Animal Reproduction
Reprodução de Caprinos
topic Animal Reproduction
Reprodução de Caprinos
description The objective of this study was to evaluate by in vitro tests and in vivo fertility, two concentrations of egg yolk (T1 = 20% or T2 = 2.5%) in egg yolk-citrate semen extender for storing goat semen at 5°C for 24 hours. Ninety semen collections were performed in four goats. The semen was split and diluted in two different semen extenders and stored in refrigerator at 5°C for 24 hours, until the semen evaluation and insemination procedures. The average values for cooled semen, respectively, for T1 and T2 were motility, 68.5 ± 15.4 and 78.0 ± 5.5 (p < 0.05); vigor, 2.5 ± 0.6 and 3.2 ± 0.3 (p < 0.05), supravital test, 59.4 ± 17.2 and 71.1 ± 10.5 (p < 0.05) and hypoosmotic test, 42.2 ± 15.7 and 56.2 ± 11.5 (p < 0.05). The fertility rate was greater (p < 0.05) in T1 (31.3%) than in T2 (66.7%) and the prolificacy did not differ (p < 0.05) between T1 (1.3 ± 0.5) and T2 (1.4 ± 0.5). In conclusion, the egg yolk-citrate extender with a low concentration (2.5%) of egg yolk was more effective in preserving the viability and fertility of goat semen stored at 5°C for 24 hours.KEYWORDS: artificial insemination; extender; goat; semen; spermatozoa.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/13115
10.5216/cab.v12i4.13115
url https://revistas.ufg.br/vet/article/view/13115
identifier_str_mv 10.5216/cab.v12i4.13115
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/13115/10036
https://revistas.ufg.br/vet/article/view/13115/10037
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 12 No. 4 (2011); 653-660
Ciência Animal Brasileira / Brazilian Animal Science; v. 12 n. 4 (2011); 653-660
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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