CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION

Detalhes bibliográficos
Autor(a) principal: Dias, Julio Cesar Oliveira
Data de Publicação: 2015
Outros Autores: Santos, Madriano Christilis da Rocha, Penitente Filho, Jurandy Mauro, Oliveira, Gisele Dias, Mendes, Vivian Rachel Araujo, Mancio, Antonio Bento
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/24240
Resumo: Cryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and strength of sperm, as well as changes in the plasma membrane after the addition of Ringer Lactate, sodium citrate 2.92% or TRIS solution in thawed goat semen. Semen was collected from two Alpine Brown goats, and standard procedures for cryopreservation and seminal analysis were performed. After thawing the semen, the extenders Ringer Lactate, sodium citrate 2.92% and TRIS solution were added and Thermoresistance (TTR), Supravital and Morphology Tests were carried out. In TTR, only the group received TRIS solution presented motility and strength for a longer period (90 minutes; P< 0,05). No influence of the addition of the solutions was found in the morphological analysis, as well as in the Supravital Test, which showed values ??close to those found for motility (P> 0.05). We concluded that the addition of the solutions does not allow a large persistence of motility and strength of thawed sperm, but TRIS solution could be used for expansion of seminal doses used in vitro reproductive biotechnology. Keywords: extender, goat, semen, spermatozoa.
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spelling CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTIONCARACTERÍSTICAS DO SÊMEN CAPRINO DESCONGELADO APÓS A ADIÇÃO DE RINGER LACTATO, CITRATO DE SÓDIO E SOLUÇÃO TRISAnimal ReproductionReprodução de CaprinosCryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and strength of sperm, as well as changes in the plasma membrane after the addition of Ringer Lactate, sodium citrate 2.92% or TRIS solution in thawed goat semen. Semen was collected from two Alpine Brown goats, and standard procedures for cryopreservation and seminal analysis were performed. After thawing the semen, the extenders Ringer Lactate, sodium citrate 2.92% and TRIS solution were added and Thermoresistance (TTR), Supravital and Morphology Tests were carried out. In TTR, only the group received TRIS solution presented motility and strength for a longer period (90 minutes; P< 0,05). No influence of the addition of the solutions was found in the morphological analysis, as well as in the Supravital Test, which showed values ??close to those found for motility (P> 0.05). We concluded that the addition of the solutions does not allow a large persistence of motility and strength of thawed sperm, but TRIS solution could be used for expansion of seminal doses used in vitro reproductive biotechnology. Keywords: extender, goat, semen, spermatozoa.A criopreservação do sêmen é de grande importância para diversas biotecnologias da reprodução, como Inseminação Artificial (IA), Produção de Embriões In Vitro (PIV) e Injeção Intracitoplasmática de Espermatozoides (ICSI). Avaliou-se a estabilidade e persistência da motilidade e vigor dos espermatozoides, assim como alterações da membrana plasmática, após a adição de Ringer com Lactato, citrato de sódio 2,92% ou solução TRIS ao sêmen caprino descongelado. O sêmen foi coletado de dois bodes da raça Parda Alpina, realizando-se os procedimentos padrões de análise e criopreservação seminal. Após a descongelação do sêmen, foram adicionados os diluentes Ringer Lactato, citrato de sódio 2,92% ou solução TRIS, realizando-se os Testes de Termorresistência (TTR), Supravital e Morfológico. No TTR, somente o grupo a que foi adicionada a Solução TRIS obteve motilidade e vigor por maior período (90 minutos; P< 0,05). Não foi encontrada influência da adição das soluções na análise morfológica, assim como no teste Supravital, o qual apresentou valores próximos aos encontrados para motilidade (P> 0,05). Concluiu-se que a adição das soluções não permite uma grande persistência da motilidade e vigor dos espermatozoides descongelados, porém a solução TRIS poderia ser utilizada para expansão de doses seminais utilizadas em biotecnologias reprodutivas in vitro. Palavras-chave: bode; diluente; espermatozoide; sêmen.Universidade Federal de Goiás2015-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2424010.1590/cab.v16i2.24240Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 2 (2015); 243-250Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 2 (2015); 243-2501809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/24240/18497https://revistas.ufg.br/vet/article/view/24240/18498Dias, Julio Cesar OliveiraSantos, Madriano Christilis da RochaPenitente Filho, Jurandy MauroOliveira, Gisele DiasMendes, Vivian Rachel AraujoMancio, Antonio Bentoinfo:eu-repo/semantics/openAccess2015-05-22T12:39:37Zoai:ojs.revistas.ufg.br:article/24240Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:59.337171Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION
CARACTERÍSTICAS DO SÊMEN CAPRINO DESCONGELADO APÓS A ADIÇÃO DE RINGER LACTATO, CITRATO DE SÓDIO E SOLUÇÃO TRIS
title CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION
spellingShingle CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION
Dias, Julio Cesar Oliveira
Animal Reproduction
Reprodução de Caprinos
title_short CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION
title_full CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION
title_fullStr CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION
title_full_unstemmed CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION
title_sort CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION
author Dias, Julio Cesar Oliveira
author_facet Dias, Julio Cesar Oliveira
Santos, Madriano Christilis da Rocha
Penitente Filho, Jurandy Mauro
Oliveira, Gisele Dias
Mendes, Vivian Rachel Araujo
Mancio, Antonio Bento
author_role author
author2 Santos, Madriano Christilis da Rocha
Penitente Filho, Jurandy Mauro
Oliveira, Gisele Dias
Mendes, Vivian Rachel Araujo
Mancio, Antonio Bento
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Dias, Julio Cesar Oliveira
Santos, Madriano Christilis da Rocha
Penitente Filho, Jurandy Mauro
Oliveira, Gisele Dias
Mendes, Vivian Rachel Araujo
Mancio, Antonio Bento
dc.subject.por.fl_str_mv Animal Reproduction
Reprodução de Caprinos
topic Animal Reproduction
Reprodução de Caprinos
description Cryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and strength of sperm, as well as changes in the plasma membrane after the addition of Ringer Lactate, sodium citrate 2.92% or TRIS solution in thawed goat semen. Semen was collected from two Alpine Brown goats, and standard procedures for cryopreservation and seminal analysis were performed. After thawing the semen, the extenders Ringer Lactate, sodium citrate 2.92% and TRIS solution were added and Thermoresistance (TTR), Supravital and Morphology Tests were carried out. In TTR, only the group received TRIS solution presented motility and strength for a longer period (90 minutes; P< 0,05). No influence of the addition of the solutions was found in the morphological analysis, as well as in the Supravital Test, which showed values ??close to those found for motility (P> 0.05). We concluded that the addition of the solutions does not allow a large persistence of motility and strength of thawed sperm, but TRIS solution could be used for expansion of seminal doses used in vitro reproductive biotechnology. Keywords: extender, goat, semen, spermatozoa.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/24240
10.1590/cab.v16i2.24240
url https://revistas.ufg.br/vet/article/view/24240
identifier_str_mv 10.1590/cab.v16i2.24240
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/24240/18497
https://revistas.ufg.br/vet/article/view/24240/18498
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 2 (2015); 243-250
Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 2 (2015); 243-250
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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