CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/24240 |
Resumo: | Cryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and strength of sperm, as well as changes in the plasma membrane after the addition of Ringer Lactate, sodium citrate 2.92% or TRIS solution in thawed goat semen. Semen was collected from two Alpine Brown goats, and standard procedures for cryopreservation and seminal analysis were performed. After thawing the semen, the extenders Ringer Lactate, sodium citrate 2.92% and TRIS solution were added and Thermoresistance (TTR), Supravital and Morphology Tests were carried out. In TTR, only the group received TRIS solution presented motility and strength for a longer period (90 minutes; P< 0,05). No influence of the addition of the solutions was found in the morphological analysis, as well as in the Supravital Test, which showed values ??close to those found for motility (P> 0.05). We concluded that the addition of the solutions does not allow a large persistence of motility and strength of thawed sperm, but TRIS solution could be used for expansion of seminal doses used in vitro reproductive biotechnology. Keywords: extender, goat, semen, spermatozoa. |
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CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTIONCARACTERÍSTICAS DO SÊMEN CAPRINO DESCONGELADO APÓS A ADIÇÃO DE RINGER LACTATO, CITRATO DE SÓDIO E SOLUÇÃO TRISAnimal ReproductionReprodução de CaprinosCryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and strength of sperm, as well as changes in the plasma membrane after the addition of Ringer Lactate, sodium citrate 2.92% or TRIS solution in thawed goat semen. Semen was collected from two Alpine Brown goats, and standard procedures for cryopreservation and seminal analysis were performed. After thawing the semen, the extenders Ringer Lactate, sodium citrate 2.92% and TRIS solution were added and Thermoresistance (TTR), Supravital and Morphology Tests were carried out. In TTR, only the group received TRIS solution presented motility and strength for a longer period (90 minutes; P< 0,05). No influence of the addition of the solutions was found in the morphological analysis, as well as in the Supravital Test, which showed values ??close to those found for motility (P> 0.05). We concluded that the addition of the solutions does not allow a large persistence of motility and strength of thawed sperm, but TRIS solution could be used for expansion of seminal doses used in vitro reproductive biotechnology. Keywords: extender, goat, semen, spermatozoa.A criopreservação do sêmen é de grande importância para diversas biotecnologias da reprodução, como Inseminação Artificial (IA), Produção de Embriões In Vitro (PIV) e Injeção Intracitoplasmática de Espermatozoides (ICSI). Avaliou-se a estabilidade e persistência da motilidade e vigor dos espermatozoides, assim como alterações da membrana plasmática, após a adição de Ringer com Lactato, citrato de sódio 2,92% ou solução TRIS ao sêmen caprino descongelado. O sêmen foi coletado de dois bodes da raça Parda Alpina, realizando-se os procedimentos padrões de análise e criopreservação seminal. Após a descongelação do sêmen, foram adicionados os diluentes Ringer Lactato, citrato de sódio 2,92% ou solução TRIS, realizando-se os Testes de Termorresistência (TTR), Supravital e Morfológico. No TTR, somente o grupo a que foi adicionada a Solução TRIS obteve motilidade e vigor por maior período (90 minutos; P< 0,05). Não foi encontrada influência da adição das soluções na análise morfológica, assim como no teste Supravital, o qual apresentou valores próximos aos encontrados para motilidade (P> 0,05). Concluiu-se que a adição das soluções não permite uma grande persistência da motilidade e vigor dos espermatozoides descongelados, porém a solução TRIS poderia ser utilizada para expansão de doses seminais utilizadas em biotecnologias reprodutivas in vitro. Palavras-chave: bode; diluente; espermatozoide; sêmen.Universidade Federal de Goiás2015-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2424010.1590/cab.v16i2.24240Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 2 (2015); 243-250Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 2 (2015); 243-2501809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/24240/18497https://revistas.ufg.br/vet/article/view/24240/18498Dias, Julio Cesar OliveiraSantos, Madriano Christilis da RochaPenitente Filho, Jurandy MauroOliveira, Gisele DiasMendes, Vivian Rachel AraujoMancio, Antonio Bentoinfo:eu-repo/semantics/openAccess2015-05-22T12:39:37Zoai:ojs.revistas.ufg.br:article/24240Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:59.337171Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION CARACTERÍSTICAS DO SÊMEN CAPRINO DESCONGELADO APÓS A ADIÇÃO DE RINGER LACTATO, CITRATO DE SÓDIO E SOLUÇÃO TRIS |
title |
CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION |
spellingShingle |
CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION Dias, Julio Cesar Oliveira Animal Reproduction Reprodução de Caprinos |
title_short |
CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION |
title_full |
CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION |
title_fullStr |
CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION |
title_full_unstemmed |
CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION |
title_sort |
CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION |
author |
Dias, Julio Cesar Oliveira |
author_facet |
Dias, Julio Cesar Oliveira Santos, Madriano Christilis da Rocha Penitente Filho, Jurandy Mauro Oliveira, Gisele Dias Mendes, Vivian Rachel Araujo Mancio, Antonio Bento |
author_role |
author |
author2 |
Santos, Madriano Christilis da Rocha Penitente Filho, Jurandy Mauro Oliveira, Gisele Dias Mendes, Vivian Rachel Araujo Mancio, Antonio Bento |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Dias, Julio Cesar Oliveira Santos, Madriano Christilis da Rocha Penitente Filho, Jurandy Mauro Oliveira, Gisele Dias Mendes, Vivian Rachel Araujo Mancio, Antonio Bento |
dc.subject.por.fl_str_mv |
Animal Reproduction Reprodução de Caprinos |
topic |
Animal Reproduction Reprodução de Caprinos |
description |
Cryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and strength of sperm, as well as changes in the plasma membrane after the addition of Ringer Lactate, sodium citrate 2.92% or TRIS solution in thawed goat semen. Semen was collected from two Alpine Brown goats, and standard procedures for cryopreservation and seminal analysis were performed. After thawing the semen, the extenders Ringer Lactate, sodium citrate 2.92% and TRIS solution were added and Thermoresistance (TTR), Supravital and Morphology Tests were carried out. In TTR, only the group received TRIS solution presented motility and strength for a longer period (90 minutes; P< 0,05). No influence of the addition of the solutions was found in the morphological analysis, as well as in the Supravital Test, which showed values ??close to those found for motility (P> 0.05). We concluded that the addition of the solutions does not allow a large persistence of motility and strength of thawed sperm, but TRIS solution could be used for expansion of seminal doses used in vitro reproductive biotechnology. Keywords: extender, goat, semen, spermatozoa. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-04-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Avaliado por pares Pesquisa Científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/24240 10.1590/cab.v16i2.24240 |
url |
https://revistas.ufg.br/vet/article/view/24240 |
identifier_str_mv |
10.1590/cab.v16i2.24240 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/24240/18497 https://revistas.ufg.br/vet/article/view/24240/18498 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 2 (2015); 243-250 Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 2 (2015); 243-250 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874788149166080 |