SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATION

Detalhes bibliográficos
Autor(a) principal: Barboza Guimarães, Daianny
Data de Publicação: 2018
Outros Autores: Bandeira Barros, Tatyane, Furtado Cantanhêde, Ludymila, Nguekam Feugang, Jean Magloire, Peres de Souza, Leonardo, Toniolli, Ricardo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/e-38250
Resumo: Semen cryopreservation is associated with low productivity results. This study aimed to test the Coconut Water Powder (ACP®-103) as a ressuspension diluent after thawing semen, also evaluate sperm quality during the cooling curve until thawing of the semen. For this, the semen was collected from 15 boars once a week, incubated at 30 °C for 15 minutes, and afterwards, the samples were diluted in the diluent Beltsville Thawing Solution – BTS (Control) or ACP-103®, and subjected to a slow cooling curve, where the force and the motility were analyzed in each step. The thawed semen was ressuspended in its respective solvents and analyzed in the characteristics of vigor, motility, vitality, acrosome integrity and functionality of the membrane. During the analysis of vigor and motility that make up the cooling curve, and thawing, for analysis of vitality and intact acrosomal membrane, it was observed that there was no significant difference between treatments. Besides, after thawing, the BTS showed better results of sperm vigor, sperm motility and membrane functionality. However, the cooling curve and coconut water powder can be used in the protocol for cryopreservation of boar semen, as both ensured quality of sperm viability that may be used in artificial insemination.Keywords: Swine semen; alternative extender; freezing; sperm viability.
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spelling SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATIONQualidade espermática durante a curva de resfriamento do sêmen suíno diluído em água de coco em pó visando sua criopreservaçãoSêmen suínoDiluente alternativeCongelaçãoViabilidade espermáticaSemen cryopreservation is associated with low productivity results. This study aimed to test the Coconut Water Powder (ACP®-103) as a ressuspension diluent after thawing semen, also evaluate sperm quality during the cooling curve until thawing of the semen. For this, the semen was collected from 15 boars once a week, incubated at 30 °C for 15 minutes, and afterwards, the samples were diluted in the diluent Beltsville Thawing Solution – BTS (Control) or ACP-103®, and subjected to a slow cooling curve, where the force and the motility were analyzed in each step. The thawed semen was ressuspended in its respective solvents and analyzed in the characteristics of vigor, motility, vitality, acrosome integrity and functionality of the membrane. During the analysis of vigor and motility that make up the cooling curve, and thawing, for analysis of vitality and intact acrosomal membrane, it was observed that there was no significant difference between treatments. Besides, after thawing, the BTS showed better results of sperm vigor, sperm motility and membrane functionality. However, the cooling curve and coconut water powder can be used in the protocol for cryopreservation of boar semen, as both ensured quality of sperm viability that may be used in artificial insemination.Keywords: Swine semen; alternative extender; freezing; sperm viability.A criopreservação seminal apresenta baixos resultados produtivos. Objetivou-se testar a Água de Coco em Pó (ACP-103®) como diluente de ressuspensão após a descongelação seminal e avaliar a qualidade espermática durante a curva de resfriamento até a descongelação do sêmen. Para isso, o sêmen de 15 reprodutores foi coletado uma vez por semana, incubado a 30 oC por 15 minutos, e em seguida diluído em Beltsville Thawing Solution – BTS (controle) ou em ACP-103®, e submetidos a uma curva de resfriamento lenta, onde foram feitas análises de vigor e motilidade em cada passo. O sêmen descongelado foi ressuspenso em seus respectivos diluentes e analisado quanto às características: vigor, motilidade, vitalidade, integridade acrossomal e funcionalidade da membrana. Durante as análises de vigor e motilidade que compõem a curva de resfriamento, e na descongelação, para as análises de vitalidade e membrana acrossomal intacta, observou-se que não houve diferença significativa entre os tratamentos. Já após a descongelação, o BTS apresentou melhores resultados de vigor, motilidade espermática e funcionalidade da membrana. No entanto, a curva de resfriamento e o ACP-103® podem ser utilizadas no protocolo de criopreservação do sêmen suíno, visto que ambas asseguraram qualidade da viabilidade espermática.Universidade Federal de Goiás2018-01-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa CientíficaAnimal Reproductionapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/e-3825010.1590/cab19138250Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-16Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-161809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/e-38250/25078https://revistas.ufg.br/vet/article/view/e-38250/25079Barboza Guimarães, DaiannyBandeira Barros, TatyaneFurtado Cantanhêde, LudymilaNguekam Feugang, Jean MagloirePeres de Souza, LeonardoToniolli, Ricardoinfo:eu-repo/semantics/openAccess2023-03-23T18:50:39Zoai:ojs.revistas.ufg.br:article/38250Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:12.248485Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATION
Qualidade espermática durante a curva de resfriamento do sêmen suíno diluído em água de coco em pó visando sua criopreservação
title SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATION
spellingShingle SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATION
Barboza Guimarães, Daianny
Sêmen suíno
Diluente alternative
Congelação
Viabilidade espermática
title_short SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATION
title_full SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATION
title_fullStr SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATION
title_full_unstemmed SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATION
title_sort SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATION
author Barboza Guimarães, Daianny
author_facet Barboza Guimarães, Daianny
Bandeira Barros, Tatyane
Furtado Cantanhêde, Ludymila
Nguekam Feugang, Jean Magloire
Peres de Souza, Leonardo
Toniolli, Ricardo
author_role author
author2 Bandeira Barros, Tatyane
Furtado Cantanhêde, Ludymila
Nguekam Feugang, Jean Magloire
Peres de Souza, Leonardo
Toniolli, Ricardo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Barboza Guimarães, Daianny
Bandeira Barros, Tatyane
Furtado Cantanhêde, Ludymila
Nguekam Feugang, Jean Magloire
Peres de Souza, Leonardo
Toniolli, Ricardo
dc.subject.por.fl_str_mv Sêmen suíno
Diluente alternative
Congelação
Viabilidade espermática
topic Sêmen suíno
Diluente alternative
Congelação
Viabilidade espermática
description Semen cryopreservation is associated with low productivity results. This study aimed to test the Coconut Water Powder (ACP®-103) as a ressuspension diluent after thawing semen, also evaluate sperm quality during the cooling curve until thawing of the semen. For this, the semen was collected from 15 boars once a week, incubated at 30 °C for 15 minutes, and afterwards, the samples were diluted in the diluent Beltsville Thawing Solution – BTS (Control) or ACP-103®, and subjected to a slow cooling curve, where the force and the motility were analyzed in each step. The thawed semen was ressuspended in its respective solvents and analyzed in the characteristics of vigor, motility, vitality, acrosome integrity and functionality of the membrane. During the analysis of vigor and motility that make up the cooling curve, and thawing, for analysis of vitality and intact acrosomal membrane, it was observed that there was no significant difference between treatments. Besides, after thawing, the BTS showed better results of sperm vigor, sperm motility and membrane functionality. However, the cooling curve and coconut water powder can be used in the protocol for cryopreservation of boar semen, as both ensured quality of sperm viability that may be used in artificial insemination.Keywords: Swine semen; alternative extender; freezing; sperm viability.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa Científica
Animal Reproduction
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/e-38250
10.1590/cab19138250
url https://revistas.ufg.br/vet/article/view/e-38250
identifier_str_mv 10.1590/cab19138250
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/e-38250/25078
https://revistas.ufg.br/vet/article/view/e-38250/25079
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-16
Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-16
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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