ADDITION OF SEMINAL PLASMA TO FROZEN-THAWED RAM SEMEN

Detalhes bibliográficos
Autor(a) principal: Rocha, Liana Vieira
Data de Publicação: 2011
Outros Autores: Dias, Angelo Burla, Quirino, Célia Raquel
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/3344
Resumo: The considerable variability in constituents of seminal plasma could explain the particular differences in responses to freezing and in fertility rates after artificial insemination in ovines. This study assessed the effect of a pool of concentrated seminal plasma on ovine semen after thawing. Ejaculates of 12 Santa Inês rams were collected using an artificial vagina, diluted in Tris/egg yolk /glycerol and frozen in a semen and embryo freezer. After thawing, sperms were incubated using concentrated seminal plasma (10X) for 15 min. Semen was submitted to biochemical analysis, followed by a computerized assessment of its physical characteristics, hypoosmotic test and heat resistance evaluation. The electrophoretic analysis of seminal plasma indicated particular differences in the samples protein composition, which were attenuated by the seminal plasma pool. The samples treated with seminal plasma showed progressive reduced motility. No statistically significant difference was observed in the other semen characteristics (p<0.05), between samples treated with seminal plasma and control samples. The concentrated seminal plasma pool did not improve the assessed semen characteristics.KEYWORDS: Rams; Freezing; Semen; Electrophoresis
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spelling ADDITION OF SEMINAL PLASMA TO FROZEN-THAWED RAM SEMENADIÇÃO DO PLASMA SEMINAL AO SÊMEN OVINO DESCONGELADOAnimal reproductionBiotecnologia da Reprodução AnimalThe considerable variability in constituents of seminal plasma could explain the particular differences in responses to freezing and in fertility rates after artificial insemination in ovines. This study assessed the effect of a pool of concentrated seminal plasma on ovine semen after thawing. Ejaculates of 12 Santa Inês rams were collected using an artificial vagina, diluted in Tris/egg yolk /glycerol and frozen in a semen and embryo freezer. After thawing, sperms were incubated using concentrated seminal plasma (10X) for 15 min. Semen was submitted to biochemical analysis, followed by a computerized assessment of its physical characteristics, hypoosmotic test and heat resistance evaluation. The electrophoretic analysis of seminal plasma indicated particular differences in the samples protein composition, which were attenuated by the seminal plasma pool. The samples treated with seminal plasma showed progressive reduced motility. No statistically significant difference was observed in the other semen characteristics (p<0.05), between samples treated with seminal plasma and control samples. The concentrated seminal plasma pool did not improve the assessed semen characteristics.KEYWORDS: Rams; Freezing; Semen; ElectrophoresisA grande variabilidade na constituição do plasma seminal pode explicar as diferenças individuais nas respostas ao congelamento e na fertilidade após a inseminação artificial em ovinos. Esse trabalho teve como objetivo avaliar o efeito da adição de um pool de plasma seminal concentrado ao sêmen ovino após descongelamento. Os ejaculados de doze carneiros da raça Santa Inês foram coletados com vagina artificial, diluídos em tris/gema/glicerol e congelados em um congelador de sêmen e embriões. Após o descongelamento foi realizada a incubação dos espermatozóides com o plasma seminal concentrado (10x), durante 15 minutos. O sêmen foi submetido à análise bioquímica, avaliação computadorizada de suas características físicas, teste hiposmótico e teste de termo-resistência. A análise eletroforética do plasma seminal apontou diferenças individuais na constituição protéica das amostras, as quais foram amenizadas com o pool de plasma seminal. Amostras tratadas com o plasma seminal apresentaram redução da motilidade progressiva. Nas demais características seminais analisadas não houve diferença  (p<0,05) entre as amostras tratadas com o plasma seminal e as amostras controle. O pool de plasma seminal concentrado não melhorou as características seminais analisadas.PALAVRAS-CHAVE: carneiros, congelamento, sêmen, eletroforeseUniversidade Federal de Goiás2011-06-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/334410.5216/cab.v12i2.3344Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 12 No. 2 (2011); 198-205Ciência Animal Brasileira / Brazilian Animal Science; v. 12 n. 2 (2011); 198-2051809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/3344/9212https://revistas.ufg.br/vet/article/view/3344/9213Rocha, Liana VieiraDias, Angelo BurlaQuirino, Célia Raquelinfo:eu-repo/semantics/openAccess2011-06-27T14:44:54Zoai:ojs.revistas.ufg.br:article/3344Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:06.790968Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv ADDITION OF SEMINAL PLASMA TO FROZEN-THAWED RAM SEMEN
ADIÇÃO DO PLASMA SEMINAL AO SÊMEN OVINO DESCONGELADO
title ADDITION OF SEMINAL PLASMA TO FROZEN-THAWED RAM SEMEN
spellingShingle ADDITION OF SEMINAL PLASMA TO FROZEN-THAWED RAM SEMEN
Rocha, Liana Vieira
Animal reproduction
Biotecnologia da Reprodução Animal
title_short ADDITION OF SEMINAL PLASMA TO FROZEN-THAWED RAM SEMEN
title_full ADDITION OF SEMINAL PLASMA TO FROZEN-THAWED RAM SEMEN
title_fullStr ADDITION OF SEMINAL PLASMA TO FROZEN-THAWED RAM SEMEN
title_full_unstemmed ADDITION OF SEMINAL PLASMA TO FROZEN-THAWED RAM SEMEN
title_sort ADDITION OF SEMINAL PLASMA TO FROZEN-THAWED RAM SEMEN
author Rocha, Liana Vieira
author_facet Rocha, Liana Vieira
Dias, Angelo Burla
Quirino, Célia Raquel
author_role author
author2 Dias, Angelo Burla
Quirino, Célia Raquel
author2_role author
author
dc.contributor.author.fl_str_mv Rocha, Liana Vieira
Dias, Angelo Burla
Quirino, Célia Raquel
dc.subject.por.fl_str_mv Animal reproduction
Biotecnologia da Reprodução Animal
topic Animal reproduction
Biotecnologia da Reprodução Animal
description The considerable variability in constituents of seminal plasma could explain the particular differences in responses to freezing and in fertility rates after artificial insemination in ovines. This study assessed the effect of a pool of concentrated seminal plasma on ovine semen after thawing. Ejaculates of 12 Santa Inês rams were collected using an artificial vagina, diluted in Tris/egg yolk /glycerol and frozen in a semen and embryo freezer. After thawing, sperms were incubated using concentrated seminal plasma (10X) for 15 min. Semen was submitted to biochemical analysis, followed by a computerized assessment of its physical characteristics, hypoosmotic test and heat resistance evaluation. The electrophoretic analysis of seminal plasma indicated particular differences in the samples protein composition, which were attenuated by the seminal plasma pool. The samples treated with seminal plasma showed progressive reduced motility. No statistically significant difference was observed in the other semen characteristics (p<0.05), between samples treated with seminal plasma and control samples. The concentrated seminal plasma pool did not improve the assessed semen characteristics.KEYWORDS: Rams; Freezing; Semen; Electrophoresis
publishDate 2011
dc.date.none.fl_str_mv 2011-06-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/3344
10.5216/cab.v12i2.3344
url https://revistas.ufg.br/vet/article/view/3344
identifier_str_mv 10.5216/cab.v12i2.3344
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/3344/9212
https://revistas.ufg.br/vet/article/view/3344/9213
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 12 No. 2 (2011); 198-205
Ciência Animal Brasileira / Brazilian Animal Science; v. 12 n. 2 (2011); 198-205
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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