COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS

Detalhes bibliográficos
Autor(a) principal: Borges, Gabriela Teixeira
Data de Publicação: 2008
Outros Autores: Dias, Miguel Joaquim, da Silva, Luiz Antônio Franco, Lima, Caroline Rocha de Oliveira, Moura, Maria Ivete, Gambarini, Maria Lúcia
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/447
Resumo: The aim of present work was to evaluate the changes of cervico-vaginal epithelial cells and progesterone levels of nuliparous goats submitted to artificial photoperiod, in Middle-West of Brazil. Seventh Alpine and six crossbreed nuliparous goats were used, distributed in two groups: control (GC), maintained at isolated stall and submitted to natural light for the time of the year, and treated (GT), submitted to an alternate regime among natural and artificial light, for 24 hours and 35 uninterrupted days. Cérvico-vaginal content was collected, prepared on glass slides and differential cellular counts were carried out on Papanicolau smears. The progesterone concentration in blood serum was measurement by ELISA. Parabasal were the most frequent cell type present, followed by intermediate cells, mainly in the end of metaestrus and diestrus. Superficial cornified cells were present during proestrus and estrus. The cellular profile and progesterone concentrations oscillated in agreement with estrus cycle phase in control animals, but confuse on the begin of experimental period for treated animals. Cytological evaluation of cervico-vaginal content could be of value for a hormonal bioassay of estrus cycle of goats submitted to artificial photoperiod just after the adaptation period.  KEY WORDS: Artificial photoperiod, caprine, hormone, vaginal cytology.
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spelling COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODSCOLPOCITOLOGIA E CONCENTRAÇÕES SÉRICAS DE PROGESTERONA EM CABRAS NULÍPARAS SUBMETIDAS AO FOTOPERÍODO ARTIFICIALThe aim of present work was to evaluate the changes of cervico-vaginal epithelial cells and progesterone levels of nuliparous goats submitted to artificial photoperiod, in Middle-West of Brazil. Seventh Alpine and six crossbreed nuliparous goats were used, distributed in two groups: control (GC), maintained at isolated stall and submitted to natural light for the time of the year, and treated (GT), submitted to an alternate regime among natural and artificial light, for 24 hours and 35 uninterrupted days. Cérvico-vaginal content was collected, prepared on glass slides and differential cellular counts were carried out on Papanicolau smears. The progesterone concentration in blood serum was measurement by ELISA. Parabasal were the most frequent cell type present, followed by intermediate cells, mainly in the end of metaestrus and diestrus. Superficial cornified cells were present during proestrus and estrus. The cellular profile and progesterone concentrations oscillated in agreement with estrus cycle phase in control animals, but confuse on the begin of experimental period for treated animals. Cytological evaluation of cervico-vaginal content could be of value for a hormonal bioassay of estrus cycle of goats submitted to artificial photoperiod just after the adaptation period.  KEY WORDS: Artificial photoperiod, caprine, hormone, vaginal cytology.O objetivo do presente trabalho foi avaliar as alterações celulares do epitélio cérvico-vaginal e as concentrações de progesterona sérica em cabras nulíparas expostas ao fotoperíodo artificial, na região Centro-Oeste do Brasil. Dezessete cabras da raça Alpina e seis mestiças, nulíparas, foram aleatoriamente distribuídas em grupo-controle (GC), mantido em galpão isolado e submetido ao regime de luz natural para a época do ano, e grupo tratado (GT), submetido ao regime alternado entre luz natural e artificial, por 24 horas durante 35 dias ininterruptos. Realizou-se colheita de conteúdo cérvico-vaginal para preparo de esfregaços, que foram corados pelo método Papanicolau. A concentração sérica de progesterona foi mensurada por enzimaimunoensaio. Verificou-se predomínio de células parabasais, seguido de células intermediárias, principalmente no final do metaestro e diestro. Células superficiais queratinizadas foram relacionadas às fases de proestro e estro. O perfil celular acompanhou as oscilações das concentrações séricas de progesterona, as quais sofreram modificações de acordo com a fase do ciclo estral nos animais-controle, mas com um período confuso no início da exposição à luz artificial, nos animais tratados. O estudo das modificações celulares do epitélio cérvico-vaginal pode ser valioso como um bioensaio hormonal do ciclo estral em cabras submetidas ao fotoperíodo artificial, apenas após o período de adaptação ao tratamento. PALAVRAS-CHAVES: Caprino, citologia vaginal, hormônio, luz artificial. Universidade Federal de Goiás2008-10-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/vet/article/view/44710.5216/cab.v9i3.447Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 9 No. 3 (2008); 759-766Ciência Animal Brasileira / Brazilian Animal Science; v. 9 n. 3 (2008); 759-7661809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/447/4148Borges, Gabriela TeixeiraDias, Miguel Joaquimda Silva, Luiz Antônio FrancoLima, Caroline Rocha de OliveiraMoura, Maria IveteGambarini, Maria Lúciainfo:eu-repo/semantics/openAccess2008-10-15T19:36:43Zoai:ojs.revistas.ufg.br:article/447Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:54:42.834253Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS
COLPOCITOLOGIA E CONCENTRAÇÕES SÉRICAS DE PROGESTERONA EM CABRAS NULÍPARAS SUBMETIDAS AO FOTOPERÍODO ARTIFICIAL
title COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS
spellingShingle COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS
Borges, Gabriela Teixeira
title_short COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS
title_full COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS
title_fullStr COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS
title_full_unstemmed COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS
title_sort COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS
author Borges, Gabriela Teixeira
author_facet Borges, Gabriela Teixeira
Dias, Miguel Joaquim
da Silva, Luiz Antônio Franco
Lima, Caroline Rocha de Oliveira
Moura, Maria Ivete
Gambarini, Maria Lúcia
author_role author
author2 Dias, Miguel Joaquim
da Silva, Luiz Antônio Franco
Lima, Caroline Rocha de Oliveira
Moura, Maria Ivete
Gambarini, Maria Lúcia
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Borges, Gabriela Teixeira
Dias, Miguel Joaquim
da Silva, Luiz Antônio Franco
Lima, Caroline Rocha de Oliveira
Moura, Maria Ivete
Gambarini, Maria Lúcia
description The aim of present work was to evaluate the changes of cervico-vaginal epithelial cells and progesterone levels of nuliparous goats submitted to artificial photoperiod, in Middle-West of Brazil. Seventh Alpine and six crossbreed nuliparous goats were used, distributed in two groups: control (GC), maintained at isolated stall and submitted to natural light for the time of the year, and treated (GT), submitted to an alternate regime among natural and artificial light, for 24 hours and 35 uninterrupted days. Cérvico-vaginal content was collected, prepared on glass slides and differential cellular counts were carried out on Papanicolau smears. The progesterone concentration in blood serum was measurement by ELISA. Parabasal were the most frequent cell type present, followed by intermediate cells, mainly in the end of metaestrus and diestrus. Superficial cornified cells were present during proestrus and estrus. The cellular profile and progesterone concentrations oscillated in agreement with estrus cycle phase in control animals, but confuse on the begin of experimental period for treated animals. Cytological evaluation of cervico-vaginal content could be of value for a hormonal bioassay of estrus cycle of goats submitted to artificial photoperiod just after the adaptation period.  KEY WORDS: Artificial photoperiod, caprine, hormone, vaginal cytology.
publishDate 2008
dc.date.none.fl_str_mv 2008-10-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/447
10.5216/cab.v9i3.447
url https://revistas.ufg.br/vet/article/view/447
identifier_str_mv 10.5216/cab.v9i3.447
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/447/4148
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 9 No. 3 (2008); 759-766
Ciência Animal Brasileira / Brazilian Animal Science; v. 9 n. 3 (2008); 759-766
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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