COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/447 |
Resumo: | The aim of present work was to evaluate the changes of cervico-vaginal epithelial cells and progesterone levels of nuliparous goats submitted to artificial photoperiod, in Middle-West of Brazil. Seventh Alpine and six crossbreed nuliparous goats were used, distributed in two groups: control (GC), maintained at isolated stall and submitted to natural light for the time of the year, and treated (GT), submitted to an alternate regime among natural and artificial light, for 24 hours and 35 uninterrupted days. Cérvico-vaginal content was collected, prepared on glass slides and differential cellular counts were carried out on Papanicolau smears. The progesterone concentration in blood serum was measurement by ELISA. Parabasal were the most frequent cell type present, followed by intermediate cells, mainly in the end of metaestrus and diestrus. Superficial cornified cells were present during proestrus and estrus. The cellular profile and progesterone concentrations oscillated in agreement with estrus cycle phase in control animals, but confuse on the begin of experimental period for treated animals. Cytological evaluation of cervico-vaginal content could be of value for a hormonal bioassay of estrus cycle of goats submitted to artificial photoperiod just after the adaptation period. KEY WORDS: Artificial photoperiod, caprine, hormone, vaginal cytology. |
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COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODSCOLPOCITOLOGIA E CONCENTRAÇÕES SÉRICAS DE PROGESTERONA EM CABRAS NULÍPARAS SUBMETIDAS AO FOTOPERÍODO ARTIFICIALThe aim of present work was to evaluate the changes of cervico-vaginal epithelial cells and progesterone levels of nuliparous goats submitted to artificial photoperiod, in Middle-West of Brazil. Seventh Alpine and six crossbreed nuliparous goats were used, distributed in two groups: control (GC), maintained at isolated stall and submitted to natural light for the time of the year, and treated (GT), submitted to an alternate regime among natural and artificial light, for 24 hours and 35 uninterrupted days. Cérvico-vaginal content was collected, prepared on glass slides and differential cellular counts were carried out on Papanicolau smears. The progesterone concentration in blood serum was measurement by ELISA. Parabasal were the most frequent cell type present, followed by intermediate cells, mainly in the end of metaestrus and diestrus. Superficial cornified cells were present during proestrus and estrus. The cellular profile and progesterone concentrations oscillated in agreement with estrus cycle phase in control animals, but confuse on the begin of experimental period for treated animals. Cytological evaluation of cervico-vaginal content could be of value for a hormonal bioassay of estrus cycle of goats submitted to artificial photoperiod just after the adaptation period. KEY WORDS: Artificial photoperiod, caprine, hormone, vaginal cytology.O objetivo do presente trabalho foi avaliar as alterações celulares do epitélio cérvico-vaginal e as concentrações de progesterona sérica em cabras nulíparas expostas ao fotoperíodo artificial, na região Centro-Oeste do Brasil. Dezessete cabras da raça Alpina e seis mestiças, nulíparas, foram aleatoriamente distribuídas em grupo-controle (GC), mantido em galpão isolado e submetido ao regime de luz natural para a época do ano, e grupo tratado (GT), submetido ao regime alternado entre luz natural e artificial, por 24 horas durante 35 dias ininterruptos. Realizou-se colheita de conteúdo cérvico-vaginal para preparo de esfregaços, que foram corados pelo método Papanicolau. A concentração sérica de progesterona foi mensurada por enzimaimunoensaio. Verificou-se predomínio de células parabasais, seguido de células intermediárias, principalmente no final do metaestro e diestro. Células superficiais queratinizadas foram relacionadas às fases de proestro e estro. O perfil celular acompanhou as oscilações das concentrações séricas de progesterona, as quais sofreram modificações de acordo com a fase do ciclo estral nos animais-controle, mas com um período confuso no início da exposição à luz artificial, nos animais tratados. O estudo das modificações celulares do epitélio cérvico-vaginal pode ser valioso como um bioensaio hormonal do ciclo estral em cabras submetidas ao fotoperíodo artificial, apenas após o período de adaptação ao tratamento. PALAVRAS-CHAVES: Caprino, citologia vaginal, hormônio, luz artificial. Universidade Federal de Goiás2008-10-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/vet/article/view/44710.5216/cab.v9i3.447Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 9 No. 3 (2008); 759-766Ciência Animal Brasileira / Brazilian Animal Science; v. 9 n. 3 (2008); 759-7661809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/447/4148Borges, Gabriela TeixeiraDias, Miguel Joaquimda Silva, Luiz Antônio FrancoLima, Caroline Rocha de OliveiraMoura, Maria IveteGambarini, Maria Lúciainfo:eu-repo/semantics/openAccess2008-10-15T19:36:43Zoai:ojs.revistas.ufg.br:article/447Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:54:42.834253Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS COLPOCITOLOGIA E CONCENTRAÇÕES SÉRICAS DE PROGESTERONA EM CABRAS NULÍPARAS SUBMETIDAS AO FOTOPERÍODO ARTIFICIAL |
title |
COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS |
spellingShingle |
COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS Borges, Gabriela Teixeira |
title_short |
COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS |
title_full |
COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS |
title_fullStr |
COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS |
title_full_unstemmed |
COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS |
title_sort |
COLPOCITOLOGY AND SERUM CONCENTRATIONS OF PROGESTERONE IN NULIPAROUS GOATS SUBMMITED TO ARTIFICIAL PHOTOPERIODS |
author |
Borges, Gabriela Teixeira |
author_facet |
Borges, Gabriela Teixeira Dias, Miguel Joaquim da Silva, Luiz Antônio Franco Lima, Caroline Rocha de Oliveira Moura, Maria Ivete Gambarini, Maria Lúcia |
author_role |
author |
author2 |
Dias, Miguel Joaquim da Silva, Luiz Antônio Franco Lima, Caroline Rocha de Oliveira Moura, Maria Ivete Gambarini, Maria Lúcia |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Borges, Gabriela Teixeira Dias, Miguel Joaquim da Silva, Luiz Antônio Franco Lima, Caroline Rocha de Oliveira Moura, Maria Ivete Gambarini, Maria Lúcia |
description |
The aim of present work was to evaluate the changes of cervico-vaginal epithelial cells and progesterone levels of nuliparous goats submitted to artificial photoperiod, in Middle-West of Brazil. Seventh Alpine and six crossbreed nuliparous goats were used, distributed in two groups: control (GC), maintained at isolated stall and submitted to natural light for the time of the year, and treated (GT), submitted to an alternate regime among natural and artificial light, for 24 hours and 35 uninterrupted days. Cérvico-vaginal content was collected, prepared on glass slides and differential cellular counts were carried out on Papanicolau smears. The progesterone concentration in blood serum was measurement by ELISA. Parabasal were the most frequent cell type present, followed by intermediate cells, mainly in the end of metaestrus and diestrus. Superficial cornified cells were present during proestrus and estrus. The cellular profile and progesterone concentrations oscillated in agreement with estrus cycle phase in control animals, but confuse on the begin of experimental period for treated animals. Cytological evaluation of cervico-vaginal content could be of value for a hormonal bioassay of estrus cycle of goats submitted to artificial photoperiod just after the adaptation period. KEY WORDS: Artificial photoperiod, caprine, hormone, vaginal cytology. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-10-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/447 10.5216/cab.v9i3.447 |
url |
https://revistas.ufg.br/vet/article/view/447 |
identifier_str_mv |
10.5216/cab.v9i3.447 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/447/4148 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 9 No. 3 (2008); 759-766 Ciência Animal Brasileira / Brazilian Animal Science; v. 9 n. 3 (2008); 759-766 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874782383046656 |