Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tde/1826 |
Resumo: | Tuberculosis is a serious public health problem all over the world. It has been demonstrated that different M. tuberculosis strains, characterized by the RFLP-IS6110 standard technique, have different virulence properties and antibiotic resistance. The aim of this study was to characterize M. tuberculosis strains isolated from patients attending two state reference hospitals of Goiânia-Goiás, using the RFLP-IS6110 technique. Positive cultures of M. tuberculosis sampled and isolated from January 2006 to June 2007 had their DNA extracted. A total of 142 viable DNA samples were analyzed, of which 126 samples presented an RFLP-IS6110 profile. Similarities comparisons between samples, as well as with literature reported profiles, were done with Bionumerics software (version 4.0). Forty three percent of the samples could be grouped in 24 clusters, when analyzed by the RFLP method, suggesting recent transmission among individuals belonging to the same cluster, however those patients did not present any epidemiological relationship, suggesting possible casual transmission between members of the same cluster. When compared with strain profile of the MDR-TB, three samples had grouped in the clade formed by families virulent. This study strengthens the importance of determining transmission routes not only within the state of Goiás but in the entire country, since there is the possibility of resistant strains transmissions. |
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KIPNIS, Andréhttp://lattes.cnpq.br/4434965360286741KIPNIS, Ana Paula Junqueirahttp://lattes.cnpq.br/1252262903952987http://lattes.cnpq.br/6790551551363826SANTOS, Lorena Cristina2014-07-29T15:30:41Z2009-08-032008-02-29SANTOS, Lorena Cristina. Molecular characterization of Mycobacterium tuberculosis isolates from patients in the city of Goiânia-GO by the technique of RFLP-IS6110. 2008. 90 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2008.http://repositorio.bc.ufg.br/tede/handle/tde/1826Tuberculosis is a serious public health problem all over the world. It has been demonstrated that different M. tuberculosis strains, characterized by the RFLP-IS6110 standard technique, have different virulence properties and antibiotic resistance. The aim of this study was to characterize M. tuberculosis strains isolated from patients attending two state reference hospitals of Goiânia-Goiás, using the RFLP-IS6110 technique. Positive cultures of M. tuberculosis sampled and isolated from January 2006 to June 2007 had their DNA extracted. A total of 142 viable DNA samples were analyzed, of which 126 samples presented an RFLP-IS6110 profile. Similarities comparisons between samples, as well as with literature reported profiles, were done with Bionumerics software (version 4.0). Forty three percent of the samples could be grouped in 24 clusters, when analyzed by the RFLP method, suggesting recent transmission among individuals belonging to the same cluster, however those patients did not present any epidemiological relationship, suggesting possible casual transmission between members of the same cluster. When compared with strain profile of the MDR-TB, three samples had grouped in the clade formed by families virulent. This study strengthens the importance of determining transmission routes not only within the state of Goiás but in the entire country, since there is the possibility of resistant strains transmissions.A tuberculose é um grave problema de saúde pública em todo o mundo. Foi demonstrado que diferentes cepas de M. tuberculosis, caracterizadas pela técnica padrão mundial, RFLP-IS6110, possuem diferentes graus de virulência e resistência a antibióticos. O objetivo deste trabalho foi caracterizar as cepas de M. tuberculosis isoladas de pacientes atendidos em dois serviços de referência na cidade de Goiânia-Goiás no período de janeiro de 2006 a junho de 2007, utilizando a técnica RFLP-IS6110. Foram processadas para cultura 175 amostras de escarro com baciloscopias positivas e um total de 142 culturas positivas para M. tuberculosis tiveram seus DNA extraídos, estes foram posteriormente submetidos a técnica de southern blotting para obtenção de seus perfis de bandas de RFLP-IS6110. Destas, 126 amostras apresentaram um perfil de RFLP-IS6110 de fácil comparação de similaridade no programa BioNumerics (versão 4.0). As amostras analisadas pelo RFLP-IS6110 permitiram o agrupamento de 43% das amostras em 24 clusters, sugerindo transmissão recente entre indivíduos agrupados, contudo estes pacientes não apresentaram nenhuma relação epidemiológica, sugerindo possíveis transmissões casuais entre membros de um mesmo cluster. Quando comparamos as amostras com linhagens MDR descritas na literatura, três se agruparam no clado formado pelas famílias virulentas. Este estudo fortaleceu a importância de se traçar cadeias de transmissões bem como a necessidade de se manter um controle de imigrantes e emigrantes visto que há a possibilidade de disseminação de cepas resistentes.Made available in DSpace on 2014-07-29T15:30:41Z (GMT). No. of bitstreams: 1 Dissertacao Lorena Cristina.pdf: 430117 bytes, checksum: 09642b3a4726659137a90dcbf634ba90 (MD5) Previous issue date: 2008-02-29application/pdfhttp://repositorio.bc.ufg.br/TEDE/retrieve/4728/Dissertacao%20Lorena%20Cristina.pdf.jpgporUniversidade Federal de GoiásMestrado em Medicina TropicalUFGBRMedicinaTuberculoseRFLP-IS61101. Tuberculose Pacientes - Goiânia(GO) 2. Tuberculose Tratamento. 3. 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dc.title.por.fl_str_mv |
Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110 |
dc.title.alternative.eng.fl_str_mv |
Molecular characterization of Mycobacterium tuberculosis isolates from patients in the city of Goiânia-GO by the technique of RFLP-IS6110 |
title |
Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110 |
spellingShingle |
Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110 SANTOS, Lorena Cristina Tuberculose RFLP-IS6110 1. Tuberculose Pacientes - Goiânia(GO) 2. Tuberculose Tratamento. 3. RFLP-IS6110 Tuberculosis RFLP-IS6110 CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
title_short |
Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110 |
title_full |
Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110 |
title_fullStr |
Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110 |
title_full_unstemmed |
Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110 |
title_sort |
Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110 |
author |
SANTOS, Lorena Cristina |
author_facet |
SANTOS, Lorena Cristina |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
KIPNIS, André |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4434965360286741 |
dc.contributor.advisor-co1.fl_str_mv |
KIPNIS, Ana Paula Junqueira |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/1252262903952987 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6790551551363826 |
dc.contributor.author.fl_str_mv |
SANTOS, Lorena Cristina |
contributor_str_mv |
KIPNIS, André KIPNIS, Ana Paula Junqueira |
dc.subject.por.fl_str_mv |
Tuberculose RFLP-IS6110 1. Tuberculose Pacientes - Goiânia(GO) 2. Tuberculose Tratamento. 3. RFLP-IS6110 |
topic |
Tuberculose RFLP-IS6110 1. Tuberculose Pacientes - Goiânia(GO) 2. Tuberculose Tratamento. 3. RFLP-IS6110 Tuberculosis RFLP-IS6110 CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
dc.subject.eng.fl_str_mv |
Tuberculosis RFLP-IS6110 |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
description |
Tuberculosis is a serious public health problem all over the world. It has been demonstrated that different M. tuberculosis strains, characterized by the RFLP-IS6110 standard technique, have different virulence properties and antibiotic resistance. The aim of this study was to characterize M. tuberculosis strains isolated from patients attending two state reference hospitals of Goiânia-Goiás, using the RFLP-IS6110 technique. Positive cultures of M. tuberculosis sampled and isolated from January 2006 to June 2007 had their DNA extracted. A total of 142 viable DNA samples were analyzed, of which 126 samples presented an RFLP-IS6110 profile. Similarities comparisons between samples, as well as with literature reported profiles, were done with Bionumerics software (version 4.0). Forty three percent of the samples could be grouped in 24 clusters, when analyzed by the RFLP method, suggesting recent transmission among individuals belonging to the same cluster, however those patients did not present any epidemiological relationship, suggesting possible casual transmission between members of the same cluster. When compared with strain profile of the MDR-TB, three samples had grouped in the clade formed by families virulent. This study strengthens the importance of determining transmission routes not only within the state of Goiás but in the entire country, since there is the possibility of resistant strains transmissions. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-02-29 |
dc.date.available.fl_str_mv |
2009-08-03 |
dc.date.accessioned.fl_str_mv |
2014-07-29T15:30:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, Lorena Cristina. Molecular characterization of Mycobacterium tuberculosis isolates from patients in the city of Goiânia-GO by the technique of RFLP-IS6110. 2008. 90 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2008. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tde/1826 |
identifier_str_mv |
SANTOS, Lorena Cristina. Molecular characterization of Mycobacterium tuberculosis isolates from patients in the city of Goiânia-GO by the technique of RFLP-IS6110. 2008. 90 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2008. |
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http://repositorio.bc.ufg.br/tede/handle/tde/1826 |
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por |
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Universidade Federal de Goiás |
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Mestrado em Medicina Tropical |
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UFG |
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BR |
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Medicina |
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Universidade Federal de Goiás |
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