Faecal Spontaneous Sedimentation Method: how many slides are really needed?

Detalhes bibliográficos
Autor(a) principal: Tibiriça, Sandra Helena Cerrato
Data de Publicação: 2009
Outros Autores: Abramo, Clarice, Simões, Alexandre Sobreira, Pinheiro, Izabella de Oliveira, Ribeiro, Luiz Cláudio, Coimbra, Elaine Soares
Tipo de documento: Artigo
Idioma: por
Título da fonte: HU Revista (Online)
Texto Completo: https://periodicos.ufjf.br/index.php/hurevista/article/view/482
Resumo: ABSTRACT This work analyzed the ideal number of slides needed for fecal spontaneous sedimentation technique, with the purpose to contribute in the diagnostic of stool test with low cost and higher benefice. In a study about intestinal parasite infection prevalence, the maximum number of slides analyzed was five slides per fecal sample, considered as a gold standard. Each slide was evaluated by different observers. Analyzing only one slide the enteroparasites positive frequency was less 60.0% and it was increased to 79.7% when analyzing the second slide. During the observation of the third and fourth slides the positive samples was 87.5 and 95.3, respectively.  It was verify that the sensibility and specificity increased with the slides number examined and during the observation of three slides per stool sample, the sensibility was 87.5% and the specificity was 99.1%. Among the enteroparasitos, the most prevalent were hookworms and Giardia lamblia, with 13.7% for both. The analyze of only one slide reveal the major risks of false negative cases. The results finding suggest the realization of three slides per stool sample for fecal spontaneous sedimentation technique, in the attempt to increase the efficiency of the fecal diagnostic methods with low costs for the public health care.
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spelling Faecal Spontaneous Sedimentation Method: how many slides are really needed?Validação do número de lâminas para realização do método de sedimentação espontânea das fezesDoenças Parasitárias. Diagnóstico. Saúde Pública.ABSTRACT This work analyzed the ideal number of slides needed for fecal spontaneous sedimentation technique, with the purpose to contribute in the diagnostic of stool test with low cost and higher benefice. In a study about intestinal parasite infection prevalence, the maximum number of slides analyzed was five slides per fecal sample, considered as a gold standard. Each slide was evaluated by different observers. Analyzing only one slide the enteroparasites positive frequency was less 60.0% and it was increased to 79.7% when analyzing the second slide. During the observation of the third and fourth slides the positive samples was 87.5 and 95.3, respectively.  It was verify that the sensibility and specificity increased with the slides number examined and during the observation of three slides per stool sample, the sensibility was 87.5% and the specificity was 99.1%. Among the enteroparasitos, the most prevalent were hookworms and Giardia lamblia, with 13.7% for both. The analyze of only one slide reveal the major risks of false negative cases. The results finding suggest the realization of three slides per stool sample for fecal spontaneous sedimentation technique, in the attempt to increase the efficiency of the fecal diagnostic methods with low costs for the public health care.Este estudo avaliou o número satisfatório de lâminas a serem examinadas pela técnica de sedimentação espontânea das fezes ou de Hoffmann, Pons e Janer (HPJ), com o intuito de contribuir para a eficiência no diagnóstico de exames coproparasitológicos no diagnóstico de parasitoses, objetivando menor custo e maior benefício. A partir de um estudo da prevalência de enteroparasitoses, cujo cálculo amostral aleatório teve como base toda a população residente no município de Piau – Minas Gerais, foram investigadas 925 amostras. O número máximo de lâminas examinadas para cada amostra de fezes foi de cinco, considerado como padrão-ouro. Cada lâmina foi avaliada por cinco examinadores diferentes. Na análise de apenas uma lâmina, a frequência de positividade para enteroparasitos foi inferior a 60% e aumentou para 79,7% na análise de uma segunda lâmina. Na observação da terceira e quarta lâminas, a positividade das amostras foi 87,5% e 95,3%, respectivamente. Verificou-se que a sensibilidade e especificidade aumentaram com o número de lâminas examinadas e que na observação de três lâminas por amostra, a sensibilidade foi de 87,5% e a especificidade de 99,1%. Entre os enteroparasitos, os ancilostomídeos e Giardia lamblia foram os mais prevalentes, representando 13,7% de positividade para ambos. A análise de apenas uma lâmina por amostra elevou o risco de resultado falso negativo. Os resultados encontrados sugerem a realização de três lâminas por amostra pela técnica do HPJ na tentativa de aumentar a acurácia do método diagnóstico com custos sustentáveis para a gestão junto ao Sistema Único de Saúde.Editora UFJF2009-11-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtOrPesquisa Experimentalapplication/pdfapplication/mswordapplication/mswordhttps://periodicos.ufjf.br/index.php/hurevista/article/view/482HU Revista; v. 35 n. 2 (2009)1982-80470103-3123reponame:HU Revista (Online)instname:Universidade Federal de Juiz de Fora (UFJF)instacron:UFJFporhttps://periodicos.ufjf.br/index.php/hurevista/article/view/482/242https://periodicos.ufjf.br/index.php/hurevista/article/view/482/15007https://periodicos.ufjf.br/index.php/hurevista/article/view/482/15010Tibiriça, Sandra Helena CerratoAbramo, ClariceSimões, Alexandre SobreiraPinheiro, Izabella de OliveiraRibeiro, Luiz CláudioCoimbra, Elaine Soaresinfo:eu-repo/semantics/openAccess2023-05-04T03:53:17Zoai:periodicos.ufjf.br:article/482Revistahttps://periodicos.ufjf.br/index.php/hurevistaPUBhttps://periodicos.ufjf.br/index.php/hurevista/oairevista.hurevista@ufjf.edu.br1982-80470103-3123opendoar:2023-05-04T03:53:17HU Revista (Online) - Universidade Federal de Juiz de Fora (UFJF)false
dc.title.none.fl_str_mv Faecal Spontaneous Sedimentation Method: how many slides are really needed?
Validação do número de lâminas para realização do método de sedimentação espontânea das fezes
title Faecal Spontaneous Sedimentation Method: how many slides are really needed?
spellingShingle Faecal Spontaneous Sedimentation Method: how many slides are really needed?
Tibiriça, Sandra Helena Cerrato
Doenças Parasitárias. Diagnóstico. Saúde Pública.
title_short Faecal Spontaneous Sedimentation Method: how many slides are really needed?
title_full Faecal Spontaneous Sedimentation Method: how many slides are really needed?
title_fullStr Faecal Spontaneous Sedimentation Method: how many slides are really needed?
title_full_unstemmed Faecal Spontaneous Sedimentation Method: how many slides are really needed?
title_sort Faecal Spontaneous Sedimentation Method: how many slides are really needed?
author Tibiriça, Sandra Helena Cerrato
author_facet Tibiriça, Sandra Helena Cerrato
Abramo, Clarice
Simões, Alexandre Sobreira
Pinheiro, Izabella de Oliveira
Ribeiro, Luiz Cláudio
Coimbra, Elaine Soares
author_role author
author2 Abramo, Clarice
Simões, Alexandre Sobreira
Pinheiro, Izabella de Oliveira
Ribeiro, Luiz Cláudio
Coimbra, Elaine Soares
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Tibiriça, Sandra Helena Cerrato
Abramo, Clarice
Simões, Alexandre Sobreira
Pinheiro, Izabella de Oliveira
Ribeiro, Luiz Cláudio
Coimbra, Elaine Soares
dc.subject.por.fl_str_mv Doenças Parasitárias. Diagnóstico. Saúde Pública.
topic Doenças Parasitárias. Diagnóstico. Saúde Pública.
description ABSTRACT This work analyzed the ideal number of slides needed for fecal spontaneous sedimentation technique, with the purpose to contribute in the diagnostic of stool test with low cost and higher benefice. In a study about intestinal parasite infection prevalence, the maximum number of slides analyzed was five slides per fecal sample, considered as a gold standard. Each slide was evaluated by different observers. Analyzing only one slide the enteroparasites positive frequency was less 60.0% and it was increased to 79.7% when analyzing the second slide. During the observation of the third and fourth slides the positive samples was 87.5 and 95.3, respectively.  It was verify that the sensibility and specificity increased with the slides number examined and during the observation of three slides per stool sample, the sensibility was 87.5% and the specificity was 99.1%. Among the enteroparasitos, the most prevalent were hookworms and Giardia lamblia, with 13.7% for both. The analyze of only one slide reveal the major risks of false negative cases. The results finding suggest the realization of three slides per stool sample for fecal spontaneous sedimentation technique, in the attempt to increase the efficiency of the fecal diagnostic methods with low costs for the public health care.
publishDate 2009
dc.date.none.fl_str_mv 2009-11-12
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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Pesquisa Experimental
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufjf.br/index.php/hurevista/article/view/482
url https://periodicos.ufjf.br/index.php/hurevista/article/view/482
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufjf.br/index.php/hurevista/article/view/482/242
https://periodicos.ufjf.br/index.php/hurevista/article/view/482/15007
https://periodicos.ufjf.br/index.php/hurevista/article/view/482/15010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Editora UFJF
publisher.none.fl_str_mv Editora UFJF
dc.source.none.fl_str_mv HU Revista; v. 35 n. 2 (2009)
1982-8047
0103-3123
reponame:HU Revista (Online)
instname:Universidade Federal de Juiz de Fora (UFJF)
instacron:UFJF
instname_str Universidade Federal de Juiz de Fora (UFJF)
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institution UFJF
reponame_str HU Revista (Online)
collection HU Revista (Online)
repository.name.fl_str_mv HU Revista (Online) - Universidade Federal de Juiz de Fora (UFJF)
repository.mail.fl_str_mv revista.hurevista@ufjf.edu.br
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