Evaluation of lugol solution used for counting rumen ciliates

Detalhes bibliográficos
Autor(a) principal: D'Agosto, Marta
Data de Publicação: 1999
Outros Autores: Carneiro, Maria Elisa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFJF
Texto Completo: http://dx.doi.org/10.1590/S0101-81751999000300011
https://repositorio.ufjf.br/jspui/handle/ufjf/9180
Resumo: The present study proposes a modification of the technique described by PURSER & MOIR (1959) for the quantitative evaluation of rumen ciliate based on an adaptation described by DEHORITY (I984). The modifying process includes: the replacement of two drops of brilliant green dye, for at least four hours, by three drops of lugol solution, for at least 15 minutes. It was made a comparative evaluation of these stainings. It was concluded that lugol solution can replace the brilliant green dye showing the following advantages: staining time reduction and subsequent speeding of sample processing; evidence of skeletal plates of entodiniomorphs making its identification easier; improved observation of small ciliates and inconspicuous structures; improved total counting and generic identification of the ciliates.
id UFJF_3f7cc9eafb74c050343db1b3f80d3b9a
oai_identifier_str oai:hermes.cpd.ufjf.br:ufjf/9180
network_acronym_str UFJF
network_name_str Repositório Institucional da UFJF
repository_id_str
spelling 2019-02-22T13:32:56Z2019-02-222019-02-22T13:32:56Z1999163725729http://dx.doi.org/10.1590/S0101-81751999000300011https://repositorio.ufjf.br/jspui/handle/ufjf/9180The present study proposes a modification of the technique described by PURSER & MOIR (1959) for the quantitative evaluation of rumen ciliate based on an adaptation described by DEHORITY (I984). The modifying process includes: the replacement of two drops of brilliant green dye, for at least four hours, by three drops of lugol solution, for at least 15 minutes. It was made a comparative evaluation of these stainings. It was concluded that lugol solution can replace the brilliant green dye showing the following advantages: staining time reduction and subsequent speeding of sample processing; evidence of skeletal plates of entodiniomorphs making its identification easier; improved observation of small ciliates and inconspicuous structures; improved total counting and generic identification of the ciliates.-eng--BrasilRevista Brasileira de Zoologia-Rumen ciliateQuantitative evaluationLugol solutionTechniqueEvaluation of lugol solution used for counting rumen ciliatesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleD'Agosto, MartaCarneiro, Maria Elisainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFJFinstname:Universidade Federal de Juiz de Fora (UFJF)instacron:UFJFTHUMBNAILEvaluation of lugol solution used for counting rumen ciliates.pdf.jpgEvaluation of lugol solution used for counting rumen ciliates.pdf.jpgGenerated Thumbnailimage/jpeg1653https://repositorio.ufjf.br/jspui/bitstream/ufjf/9180/4/Evaluation%20of%20lugol%20solution%20used%20for%20counting%20rumen%20ciliates.pdf.jpg1826127211301fc7f3ff5821e6a34ccdMD54ORIGINALEvaluation of lugol solution used for counting rumen ciliates.pdfEvaluation of lugol solution used for counting rumen ciliates.pdfapplication/pdf1216306https://repositorio.ufjf.br/jspui/bitstream/ufjf/9180/1/Evaluation%20of%20lugol%20solution%20used%20for%20counting%20rumen%20ciliates.pdf47ac59e78fd86d874e73339fd516fba7MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82197https://repositorio.ufjf.br/jspui/bitstream/ufjf/9180/2/license.txt000e18a5aee6ca21bb5811ddf55fc37bMD52TEXTEvaluation of lugol solution used for counting rumen ciliates.pdf.txtEvaluation of lugol solution used for counting rumen ciliates.pdf.txtExtracted texttext/plain7785https://repositorio.ufjf.br/jspui/bitstream/ufjf/9180/3/Evaluation%20of%20lugol%20solution%20used%20for%20counting%20rumen%20ciliates.pdf.txtee2dd7962fe6652bd4713b31445b4656MD53ufjf/91802019-06-16 12:35:02.51oai:hermes.cpd.ufjf.br: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Repositório InstitucionalPUBhttps://repositorio.ufjf.br/oai/requestopendoar:2019-06-16T15:35:02Repositório Institucional da UFJF - Universidade Federal de Juiz de Fora (UFJF)false
dc.title.pt_BR.fl_str_mv Evaluation of lugol solution used for counting rumen ciliates
title Evaluation of lugol solution used for counting rumen ciliates
spellingShingle Evaluation of lugol solution used for counting rumen ciliates
D'Agosto, Marta
-
Rumen ciliate
Quantitative evaluation
Lugol solution
Technique
title_short Evaluation of lugol solution used for counting rumen ciliates
title_full Evaluation of lugol solution used for counting rumen ciliates
title_fullStr Evaluation of lugol solution used for counting rumen ciliates
title_full_unstemmed Evaluation of lugol solution used for counting rumen ciliates
title_sort Evaluation of lugol solution used for counting rumen ciliates
author D'Agosto, Marta
author_facet D'Agosto, Marta
Carneiro, Maria Elisa
author_role author
author2 Carneiro, Maria Elisa
author2_role author
dc.contributor.author.fl_str_mv D'Agosto, Marta
Carneiro, Maria Elisa
dc.subject.cnpq.fl_str_mv -
topic -
Rumen ciliate
Quantitative evaluation
Lugol solution
Technique
dc.subject.por.fl_str_mv Rumen ciliate
Quantitative evaluation
Lugol solution
Technique
description The present study proposes a modification of the technique described by PURSER & MOIR (1959) for the quantitative evaluation of rumen ciliate based on an adaptation described by DEHORITY (I984). The modifying process includes: the replacement of two drops of brilliant green dye, for at least four hours, by three drops of lugol solution, for at least 15 minutes. It was made a comparative evaluation of these stainings. It was concluded that lugol solution can replace the brilliant green dye showing the following advantages: staining time reduction and subsequent speeding of sample processing; evidence of skeletal plates of entodiniomorphs making its identification easier; improved observation of small ciliates and inconspicuous structures; improved total counting and generic identification of the ciliates.
publishDate 1999
dc.date.issued.fl_str_mv 1999
dc.date.accessioned.fl_str_mv 2019-02-22T13:32:56Z
dc.date.available.fl_str_mv 2019-02-22
2019-02-22T13:32:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.ufjf.br/jspui/handle/ufjf/9180
dc.identifier.doi.pt_BR.fl_str_mv http://dx.doi.org/10.1590/S0101-81751999000300011
url http://dx.doi.org/10.1590/S0101-81751999000300011
https://repositorio.ufjf.br/jspui/handle/ufjf/9180
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Revista Brasileira de Zoologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv -
dc.publisher.initials.fl_str_mv -
dc.publisher.country.fl_str_mv Brasil
publisher.none.fl_str_mv -
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFJF
instname:Universidade Federal de Juiz de Fora (UFJF)
instacron:UFJF
instname_str Universidade Federal de Juiz de Fora (UFJF)
instacron_str UFJF
institution UFJF
reponame_str Repositório Institucional da UFJF
collection Repositório Institucional da UFJF
bitstream.url.fl_str_mv https://repositorio.ufjf.br/jspui/bitstream/ufjf/9180/4/Evaluation%20of%20lugol%20solution%20used%20for%20counting%20rumen%20ciliates.pdf.jpg
https://repositorio.ufjf.br/jspui/bitstream/ufjf/9180/1/Evaluation%20of%20lugol%20solution%20used%20for%20counting%20rumen%20ciliates.pdf
https://repositorio.ufjf.br/jspui/bitstream/ufjf/9180/2/license.txt
https://repositorio.ufjf.br/jspui/bitstream/ufjf/9180/3/Evaluation%20of%20lugol%20solution%20used%20for%20counting%20rumen%20ciliates.pdf.txt
bitstream.checksum.fl_str_mv 1826127211301fc7f3ff5821e6a34ccd
47ac59e78fd86d874e73339fd516fba7
000e18a5aee6ca21bb5811ddf55fc37b
ee2dd7962fe6652bd4713b31445b4656
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFJF - Universidade Federal de Juiz de Fora (UFJF)
repository.mail.fl_str_mv
_version_ 1813194040563204096

Registros relacionados