Detecção de inibidores de proteases em sementes de Punica granatum
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFJF |
| Texto Completo: | http://dx.doi.org/10.21577/0100-4042.20160189 https://repositorio.ufjf.br/jspui/handle/ufjf/7339 |
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2018-09-06T12:00:07Z2018-08-152018-09-06T12:00:07Z2017-04COLARES, Lara Franca et al .Detecção de inibidores de proteases em sementes de Punica granatum. Quím. Nova, São Paulo, v. 40, n. 3, p. 270-274, abr. 2017. Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000300270&lng=pt&nrm=iso>. acessos em 03 set. 2018. http://dx.doi.org/10.21577/0100-4042.20160189.http://dx.doi.org/10.21577/0100-4042.20160189https://repositorio.ufjf.br/jspui/handle/ufjf/7339403270274-The use of plants as a way to prevent and treat disease comes from ancient times. With the increasing return on consumption of plants for medicinal purposes, the plant-based medicines have gained greater appreciation. Protease inhibitors are compounds that can decrease activity of an enzyme. They are found in plants, especially in Fabaceae, Poaceae, and Solanaceae families, and show satisfactory use to treat diseases due to their regulatory activities and selective proteolytic degradation of target substrates. This study aimed to obtain protease inhibitors in the seeds of Punica granatum, due its use in traditional medicine. The alcohol-acid solution was used to extract and isolate the protease inhibitors in P. granatum seeds and Glycine max grains. The extract of P. granatum seeds showed similar concentration of protease inhibitors when compared to G. max. A 14 kDa band was detected by electrophoresis on polyacrylamide gel, which was characterized by high-performance liquid chromatography as protease inhibitors. The extract of P. granatum seeds showed a potent trypsin inhibitor activity (EC50 18.2 ng mL-1). This study showed for the first time the extraction, purification, and identification of protease inhibitors in Punica granatum seeds.eng--BrasilQuímica Nova-Protease inhibitorsPunica granatum seedsTraditional medicineDetecção de inibidores de proteases em sementes de Punica granatumDetection of protease inhibitors in seeds of Punica granatuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleColares, Lara FrancaSantos Neto, Antero RicardoCordeiro, Isac HenriqueCastro, Sandra Bertelli Ribeiro deVerly, Rodrigo MoreiraAlves, Caio Cesar de SouzaCarli, Alessandra de Paulainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFJFinstname:Universidade Federal de Juiz de Fora (UFJF)instacron:UFJFTHUMBNAILDetecção de inibidores de proteases em sementes de Punica granatum.pdf.jpgDetecção de inibidores de proteases em sementes de Punica granatum.pdf.jpgGenerated Thumbnailimage/jpeg1623https://repositorio.ufjf.br/jspui/bitstream/ufjf/7339/6/Detec%c3%a7%c3%a3o%20de%20inibidores%20de%20proteases%20em%20sementes%20de%20Punica%20granatum.pdf.jpg7e25359bff3cb7102c7819baa83d5aa5MD56ORIGINALDetecção de inibidores de proteases em sementes de Punica granatum.pdfDetecção de inibidores de proteases em sementes de Punica granatum.pdfapplication/pdf1183468https://repositorio.ufjf.br/jspui/bitstream/ufjf/7339/3/Detec%c3%a7%c3%a3o%20de%20inibidores%20de%20proteases%20em%20sementes%20de%20Punica%20granatum.pdffd122ac57e710b6ea8f2e08b73a6704dMD53LICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Detecção de inibidores de proteases em sementes de Punica granatum |
| dc.title.alternative.pt_BR.fl_str_mv |
Detection of protease inhibitors in seeds of Punica granatum |
| title |
Detecção de inibidores de proteases em sementes de Punica granatum |
| spellingShingle |
Detecção de inibidores de proteases em sementes de Punica granatum Colares, Lara Franca - Protease inhibitors Punica granatum seeds Traditional medicine |
| title_short |
Detecção de inibidores de proteases em sementes de Punica granatum |
| title_full |
Detecção de inibidores de proteases em sementes de Punica granatum |
| title_fullStr |
Detecção de inibidores de proteases em sementes de Punica granatum |
| title_full_unstemmed |
Detecção de inibidores de proteases em sementes de Punica granatum |
| title_sort |
Detecção de inibidores de proteases em sementes de Punica granatum |
| author |
Colares, Lara Franca |
| author_facet |
Colares, Lara Franca Santos Neto, Antero Ricardo Cordeiro, Isac Henrique Castro, Sandra Bertelli Ribeiro de Verly, Rodrigo Moreira Alves, Caio Cesar de Souza Carli, Alessandra de Paula |
| author_role |
author |
| author2 |
Santos Neto, Antero Ricardo Cordeiro, Isac Henrique Castro, Sandra Bertelli Ribeiro de Verly, Rodrigo Moreira Alves, Caio Cesar de Souza Carli, Alessandra de Paula |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Colares, Lara Franca Santos Neto, Antero Ricardo Cordeiro, Isac Henrique Castro, Sandra Bertelli Ribeiro de Verly, Rodrigo Moreira Alves, Caio Cesar de Souza Carli, Alessandra de Paula |
| dc.subject.cnpq.fl_str_mv |
- |
| topic |
- Protease inhibitors Punica granatum seeds Traditional medicine |
| dc.subject.por.fl_str_mv |
Protease inhibitors Punica granatum seeds Traditional medicine |
| description |
- |
| publishDate |
2017 |
| dc.date.issued.fl_str_mv |
2017-04 |
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2018-09-06T12:00:07Z |
| dc.date.available.fl_str_mv |
2018-08-15 2018-09-06T12:00:07Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
COLARES, Lara Franca et al .Detecção de inibidores de proteases em sementes de Punica granatum. Quím. Nova, São Paulo, v. 40, n. 3, p. 270-274, abr. 2017. Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000300270&lng=pt&nrm=iso>. acessos em 03 set. 2018. http://dx.doi.org/10.21577/0100-4042.20160189. |
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https://repositorio.ufjf.br/jspui/handle/ufjf/7339 |
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http://dx.doi.org/10.21577/0100-4042.20160189 |
| identifier_str_mv |
COLARES, Lara Franca et al .Detecção de inibidores de proteases em sementes de Punica granatum. Quím. Nova, São Paulo, v. 40, n. 3, p. 270-274, abr. 2017. Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000300270&lng=pt&nrm=iso>. acessos em 03 set. 2018. http://dx.doi.org/10.21577/0100-4042.20160189. |
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http://dx.doi.org/10.21577/0100-4042.20160189 https://repositorio.ufjf.br/jspui/handle/ufjf/7339 |
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eng |
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eng |
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Química Nova |
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openAccess |
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Brasil |
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