Quantitative and differential analysis of ciliate protozoa in rumen content samples filtered before and after fixation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFJF |
| Texto Completo: | http://dx.doi.org/10.1590/S1516-35982013001100010 https://repositorio.ufjf.br/jspui/handle/ufjf/7991 |
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2018-11-19T17:10:47Z2018-11-132018-11-19T17:10:47Z2013-114211831834http://dx.doi.org/10.1590/S1516-35982013001100010https://repositorio.ufjf.br/jspui/handle/ufjf/7991-The objective of this study was to assess whether the straining of rumen content samples influences the estimation of protozoal density. Ninety rumen samples were obtained from 30 cattle (three samples per animal). The samples were subjected to one of three treatments at the moment of collection: 1) fixation informalin without straining(control treatment), 2) straining before fixation informalin, or 3)straining after fixation informalin.To test the hypothesis of the variation in the protozoa composition in the samples, multivariate analyses with non-metric multidimensional scaling (NMDS) were carried out. The diversity and density of rumen protozoa were negatively affected by straining before fixation.In the pre-filtered sample, the number of ciliates from the genus Entodinium was reduced, and no individuals from the Diploplastron, Elytroplastron and Eudiplodinium genera were detected; these effects were not observed in the other two treatments. Straining after fixation did not interfere with the diversity of the ruminal community, but the abundance of protozoa was greater thanin the control treatment and significantly greater than in the samples filtered before fixation. These factors suggest that post-fixation straining is the recommended technique to analyze rumen protozoa.eng--BrasilRevista Brasileira de Zootecnia-CiliophoraEntodiniumRuminal protozoaRuminantQuantitative and differential analysis of ciliate protozoa in rumen content samples filtered before and after fixationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRossi, Mariana FonsecaMartinele, IsabelD'Agosto, Martainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFJFinstname:Universidade Federal de Juiz de Fora (UFJF)instacron:UFJFTHUMBNAILQuantitative and differential analysis of ciliate protozoa in rumen content samples.pdf.jpgQuantitative and differential analysis of ciliate protozoa in rumen content samples.pdf.jpgGenerated Thumbnailimage/jpeg1745https://repositorio.ufjf.br/jspui/bitstream/ufjf/7991/4/Quantitative%20and%20differential%20analysis%20of%20ciliate%20protozoa%20in%20rumen%20content%20samples.pdf.jpgc2da45191f9692a4c751ef50c1f652bfMD54ORIGINALQuantitative and differential analysis of ciliate protozoa in rumen content samples.pdfQuantitative and differential analysis of ciliate protozoa in rumen content samples.pdfapplication/pdf303069https://repositorio.ufjf.br/jspui/bitstream/ufjf/7991/1/Quantitative%20and%20differential%20analysis%20of%20ciliate%20protozoa%20in%20rumen%20content%20samples.pdf97a58a0957ea7e9fe64ed6944039866aMD51LICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Quantitative and differential analysis of ciliate protozoa in rumen content samples filtered before and after fixation |
| title |
Quantitative and differential analysis of ciliate protozoa in rumen content samples filtered before and after fixation |
| spellingShingle |
Quantitative and differential analysis of ciliate protozoa in rumen content samples filtered before and after fixation Rossi, Mariana Fonseca - Ciliophora Entodinium Ruminal protozoa Ruminant |
| title_short |
Quantitative and differential analysis of ciliate protozoa in rumen content samples filtered before and after fixation |
| title_full |
Quantitative and differential analysis of ciliate protozoa in rumen content samples filtered before and after fixation |
| title_fullStr |
Quantitative and differential analysis of ciliate protozoa in rumen content samples filtered before and after fixation |
| title_full_unstemmed |
Quantitative and differential analysis of ciliate protozoa in rumen content samples filtered before and after fixation |
| title_sort |
Quantitative and differential analysis of ciliate protozoa in rumen content samples filtered before and after fixation |
| author |
Rossi, Mariana Fonseca |
| author_facet |
Rossi, Mariana Fonseca Martinele, Isabel D'Agosto, Marta |
| author_role |
author |
| author2 |
Martinele, Isabel D'Agosto, Marta |
| author2_role |
author author |
| dc.contributor.author.fl_str_mv |
Rossi, Mariana Fonseca Martinele, Isabel D'Agosto, Marta |
| dc.subject.cnpq.fl_str_mv |
- |
| topic |
- Ciliophora Entodinium Ruminal protozoa Ruminant |
| dc.subject.por.fl_str_mv |
Ciliophora Entodinium Ruminal protozoa Ruminant |
| description |
- |
| publishDate |
2013 |
| dc.date.issued.fl_str_mv |
2013-11 |
| dc.date.accessioned.fl_str_mv |
2018-11-19T17:10:47Z |
| dc.date.available.fl_str_mv |
2018-11-13 2018-11-19T17:10:47Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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https://repositorio.ufjf.br/jspui/handle/ufjf/7991 |
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http://dx.doi.org/10.1590/S1516-35982013001100010 |
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http://dx.doi.org/10.1590/S1516-35982013001100010 https://repositorio.ufjf.br/jspui/handle/ufjf/7991 |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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Revista Brasileira de Zootecnia |
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info:eu-repo/semantics/openAccess |
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openAccess |
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- |
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Brasil |
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reponame:Repositório Institucional da UFJF instname:Universidade Federal de Juiz de Fora (UFJF) instacron:UFJF |
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Universidade Federal de Juiz de Fora (UFJF) |
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UFJF |
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Repositório Institucional da UFJF |
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