IN VITRO ESTABLISHMENT OF Peltophorum dubium ((Spreng.) Taub.) IN FUNCTION OF CONCENTRATIONS OF THE MS MEDIUM
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Cerne (Online) |
Texto Completo: | https://cerne.ufla.br/site/index.php/CERNE/article/view/87 |
Resumo: | The objective of this study was to evaluate the influence of several concentrations of MS medium on in vitro establishment of Peltophorum dubium shoot apical segments (80-100 mm) isolated from seedlings germinated under aseptic conditions. The salts concentrations 50, 75, 100, 125 and 150% of MS medium, were supplemented with 30 g L-1 sucrose, 100 mg L-1 of myo-inositol, 7 g L-1 agar and pH adjusted to 5.7. The experimental design was completely randomized, with 20 replications, each consisting of a vial containing one explant. The cultures were maintained for 60 days in culture room under 16-hour photoperiod, light intensity of 20 μmol m-2 s-1 and temperature of 25±2°C. There was no effect of different concentrations of MS medium for most variables. High average of in vitro survival (98%) and establishment (98%) were observed. The largest number of leaves (2.8) occurred at a dilution of 75%, decreasing from this point. At the same concentration the highest callus induction was observed, which was decreasing until not be observed under 125% of original composition; at 150% there was, again, the presence of calli. It was not observed rooting of explants during in vitro cultivation. Considering the set of variables and, additionally, that the presence of calli is not desirable, it is recommended the use of the original concentration of salts from the MS nutrient medium for the in vitro establishment of P. dubium shoot apicals segments. |
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IN VITRO ESTABLISHMENT OF Peltophorum dubium ((Spreng.) Taub.) IN FUNCTION OF CONCENTRATIONS OF THE MS MEDIUMTissue cultureshoot apical segmentsforestry speciesThe objective of this study was to evaluate the influence of several concentrations of MS medium on in vitro establishment of Peltophorum dubium shoot apical segments (80-100 mm) isolated from seedlings germinated under aseptic conditions. The salts concentrations 50, 75, 100, 125 and 150% of MS medium, were supplemented with 30 g L-1 sucrose, 100 mg L-1 of myo-inositol, 7 g L-1 agar and pH adjusted to 5.7. The experimental design was completely randomized, with 20 replications, each consisting of a vial containing one explant. The cultures were maintained for 60 days in culture room under 16-hour photoperiod, light intensity of 20 μmol m-2 s-1 and temperature of 25±2°C. There was no effect of different concentrations of MS medium for most variables. High average of in vitro survival (98%) and establishment (98%) were observed. The largest number of leaves (2.8) occurred at a dilution of 75%, decreasing from this point. At the same concentration the highest callus induction was observed, which was decreasing until not be observed under 125% of original composition; at 150% there was, again, the presence of calli. It was not observed rooting of explants during in vitro cultivation. Considering the set of variables and, additionally, that the presence of calli is not desirable, it is recommended the use of the original concentration of salts from the MS nutrient medium for the in vitro establishment of P. dubium shoot apicals segments.CERNECERNE2015-05-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://cerne.ufla.br/site/index.php/CERNE/article/view/87CERNE; Vol. 17 No. 4 (2011); 549-554CERNE; v. 17 n. 4 (2011); 549-5542317-63420104-7760reponame:Cerne (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLAporhttps://cerne.ufla.br/site/index.php/CERNE/article/view/87/69Copyright (c) 2015 Andressa Vasconcelos Flôres, Lia Rejane Silveira Reiniger, Aline Ritter Curti, Aline Paim, Josiana Scherer Bassan, Ana Catarina Monteiro Carvalho Mori da Cunhainfo:eu-repo/semantics/openAccessFlôres, Andressa VasconcelosReiniger, Lia Rejane SilveiraCurti, Aline RitterPaim, AlineBassan, Josiana SchererCunha, Ana Catarina Monteiro Carvalho Mori da2015-11-06T18:31:49Zoai:cerne.ufla.br:article/87Revistahttps://cerne.ufla.br/site/index.php/CERNEPUBhttps://cerne.ufla.br/site/index.php/CERNE/oaicerne@dcf.ufla.br||cerne@dcf.ufla.br2317-63420104-7760opendoar:2024-05-21T19:53:29.816654Cerne (Online) - Universidade Federal de Lavras (UFLA)true |
dc.title.none.fl_str_mv |
IN VITRO ESTABLISHMENT OF Peltophorum dubium ((Spreng.) Taub.) IN FUNCTION OF CONCENTRATIONS OF THE MS MEDIUM |
title |
IN VITRO ESTABLISHMENT OF Peltophorum dubium ((Spreng.) Taub.) IN FUNCTION OF CONCENTRATIONS OF THE MS MEDIUM |
spellingShingle |
IN VITRO ESTABLISHMENT OF Peltophorum dubium ((Spreng.) Taub.) IN FUNCTION OF CONCENTRATIONS OF THE MS MEDIUM Flôres, Andressa Vasconcelos Tissue culture shoot apical segments forestry species |
title_short |
IN VITRO ESTABLISHMENT OF Peltophorum dubium ((Spreng.) Taub.) IN FUNCTION OF CONCENTRATIONS OF THE MS MEDIUM |
title_full |
IN VITRO ESTABLISHMENT OF Peltophorum dubium ((Spreng.) Taub.) IN FUNCTION OF CONCENTRATIONS OF THE MS MEDIUM |
title_fullStr |
IN VITRO ESTABLISHMENT OF Peltophorum dubium ((Spreng.) Taub.) IN FUNCTION OF CONCENTRATIONS OF THE MS MEDIUM |
title_full_unstemmed |
IN VITRO ESTABLISHMENT OF Peltophorum dubium ((Spreng.) Taub.) IN FUNCTION OF CONCENTRATIONS OF THE MS MEDIUM |
title_sort |
IN VITRO ESTABLISHMENT OF Peltophorum dubium ((Spreng.) Taub.) IN FUNCTION OF CONCENTRATIONS OF THE MS MEDIUM |
author |
Flôres, Andressa Vasconcelos |
author_facet |
Flôres, Andressa Vasconcelos Reiniger, Lia Rejane Silveira Curti, Aline Ritter Paim, Aline Bassan, Josiana Scherer Cunha, Ana Catarina Monteiro Carvalho Mori da |
author_role |
author |
author2 |
Reiniger, Lia Rejane Silveira Curti, Aline Ritter Paim, Aline Bassan, Josiana Scherer Cunha, Ana Catarina Monteiro Carvalho Mori da |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Flôres, Andressa Vasconcelos Reiniger, Lia Rejane Silveira Curti, Aline Ritter Paim, Aline Bassan, Josiana Scherer Cunha, Ana Catarina Monteiro Carvalho Mori da |
dc.subject.por.fl_str_mv |
Tissue culture shoot apical segments forestry species |
topic |
Tissue culture shoot apical segments forestry species |
description |
The objective of this study was to evaluate the influence of several concentrations of MS medium on in vitro establishment of Peltophorum dubium shoot apical segments (80-100 mm) isolated from seedlings germinated under aseptic conditions. The salts concentrations 50, 75, 100, 125 and 150% of MS medium, were supplemented with 30 g L-1 sucrose, 100 mg L-1 of myo-inositol, 7 g L-1 agar and pH adjusted to 5.7. The experimental design was completely randomized, with 20 replications, each consisting of a vial containing one explant. The cultures were maintained for 60 days in culture room under 16-hour photoperiod, light intensity of 20 μmol m-2 s-1 and temperature of 25±2°C. There was no effect of different concentrations of MS medium for most variables. High average of in vitro survival (98%) and establishment (98%) were observed. The largest number of leaves (2.8) occurred at a dilution of 75%, decreasing from this point. At the same concentration the highest callus induction was observed, which was decreasing until not be observed under 125% of original composition; at 150% there was, again, the presence of calli. It was not observed rooting of explants during in vitro cultivation. Considering the set of variables and, additionally, that the presence of calli is not desirable, it is recommended the use of the original concentration of salts from the MS nutrient medium for the in vitro establishment of P. dubium shoot apicals segments. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-05-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://cerne.ufla.br/site/index.php/CERNE/article/view/87 |
url |
https://cerne.ufla.br/site/index.php/CERNE/article/view/87 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://cerne.ufla.br/site/index.php/CERNE/article/view/87/69 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
CERNE CERNE |
publisher.none.fl_str_mv |
CERNE CERNE |
dc.source.none.fl_str_mv |
CERNE; Vol. 17 No. 4 (2011); 549-554 CERNE; v. 17 n. 4 (2011); 549-554 2317-6342 0104-7760 reponame:Cerne (Online) instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Cerne (Online) |
collection |
Cerne (Online) |
repository.name.fl_str_mv |
Cerne (Online) - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
cerne@dcf.ufla.br||cerne@dcf.ufla.br |
_version_ |
1799874939170324480 |