Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides

Detalhes bibliográficos
Autor(a) principal: Maia, Fernanda Gonçalves Martins
Data de Publicação: 2012
Outros Autores: Ogoshi, Claudio, Pierre, Rosana Oliveira, Vieira, Jucilayne Fernandes, Ribeiro junior, Pedro Martins, Abreu, Mario Sobral
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Coffee Science (Online)
Texto Completo: https://coffeescience.ufla.br/index.php/Coffeescience/article/view/248
Resumo: The objective of this work was to study the effects of different isolates of Colletotrichum gloeosporioides, as topigment level (chlorophyll a, b and total), total soluble phenols and soluble lignin in coffee seedlings obtained by artificially inoculatedtissue culture. The experiment was carried out in randomized block design in a 2 x 3 factorial scheme (2 genetic materials), 3 isolates(an isolate from mango fruits and another one from coffee plants), with 5 replicates and control (plants without inoculation). Theplants were inoculated with a suspension of 2 x 106 conidia.ml-1. The conidial suspension was sprayed on previously injured leaves.Leaves of inoculated plants were collected at 3 and 7 days after inoculation, except for leaves on the lower part of the plants.Afterwards, samples were prepared for the evaluation of chlorophyll, total soluble phenols and soluble lignin. The results showedthat the presence of the pathogen in healthy and diseased plants significantly reduced the amount of chlorophyll. At 7 days afterinoculation of healthy and diseased coffee plants higher total soluble phenol and soluble lignin levels were observed in response to theattack by Colletotrichum gloeosporioides.
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spelling Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioidesPigmentos, fenóis solúveis totais e lignina em plântulas de cafeeiro inoculadas com Colletotrichum gloeosporioidesCoffea arabicaresponse defenseCoffea arabicaColletotrichum gloeosporioidesresposta de defesaThe objective of this work was to study the effects of different isolates of Colletotrichum gloeosporioides, as topigment level (chlorophyll a, b and total), total soluble phenols and soluble lignin in coffee seedlings obtained by artificially inoculatedtissue culture. The experiment was carried out in randomized block design in a 2 x 3 factorial scheme (2 genetic materials), 3 isolates(an isolate from mango fruits and another one from coffee plants), with 5 replicates and control (plants without inoculation). Theplants were inoculated with a suspension of 2 x 106 conidia.ml-1. The conidial suspension was sprayed on previously injured leaves.Leaves of inoculated plants were collected at 3 and 7 days after inoculation, except for leaves on the lower part of the plants.Afterwards, samples were prepared for the evaluation of chlorophyll, total soluble phenols and soluble lignin. The results showedthat the presence of the pathogen in healthy and diseased plants significantly reduced the amount of chlorophyll. At 7 days afterinoculation of healthy and diseased coffee plants higher total soluble phenol and soluble lignin levels were observed in response to theattack by Colletotrichum gloeosporioides.Objetivou-se, neste trabalho, estudar os efeitos de diferentes isolados de Colletotrichum gloeosporioides sobre osteores de pigmentos (clorofila a, b e total), fenóis solúveis totais e lignina solúvel, em dois diferentes tempos, sobre mudas de caféobtidas por cultura de tecidos e inoculadas artificialmente. O experimento foi conduzido em blocos casualizados em esquemafatorial 2 x 3 (2 materiais génticos), 3 isolados (um obtido de mangueira e 2 isolados obtidos de plantas de café) + 2 (testemunhassem inoculação), com 5 repetições. As plantas foram inoculadas com uma suspensão de 2 x 106 conídios.ml-1. A suspensão deconídios foi pulverizada sobre folhas de cafeeiro, previamente feridas. Foram realizadas duas coletas, aos 3 e 7 dias apósinoculação, de todas as folhas da muda, exceto as folhas baixeiras. Em seguida, foi feito o preparo das amostras para avaliaçãode clorofila, fenóis solúveis totais e lignina solúvel. A partir dos resultados, pode-se concluir que a presença do patógeno, tantoem plantas sadias, como em plantas doentes diminuíram significativamente a quantidade de clorofila. Aos 7 dias após inoculaçãodas plantas de café sadias e doentes, observou-se um maior teor de fenóis solúveis totais e lignina solúvel em resposta ao ataquede Colletotrichum gloeosporioides.Editora UFLA2012-08-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://coffeescience.ufla.br/index.php/Coffeescience/article/view/248Coffee Science - ISSN 1984-3909; Vol. 7 No. 2 (2012); 152-159Coffee Science; Vol. 7 Núm. 2 (2012); 152-159Coffee Science; v. 7 n. 2 (2012); 152-1591984-3909reponame:Coffee Science (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLAenghttps://coffeescience.ufla.br/index.php/Coffeescience/article/view/248/pdfCopyright (c) 2012 Coffee Science - ISSN 1984-3909https://creativecommons.org/info:eu-repo/semantics/openAccessMaia, Fernanda Gonçalves MartinsOgoshi, ClaudioPierre, Rosana OliveiraVieira, Jucilayne FernandesRibeiro junior, Pedro MartinsAbreu, Mario Sobral2013-02-24T13:15:48Zoai:coffeescience.ufla.br:article/248Revistahttps://coffeescience.ufla.br/index.php/CoffeesciencePUBhttps://coffeescience.ufla.br/index.php/Coffeescience/oaicoffeescience@dag.ufla.br||coffeescience@dag.ufla.br|| alvaro-cozadi@hotmail.com1984-39091809-6875opendoar:2024-05-21T19:53:35.435646Coffee Science (Online) - Universidade Federal de Lavras (UFLA)true
dc.title.none.fl_str_mv Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
Pigmentos, fenóis solúveis totais e lignina em plântulas de cafeeiro inoculadas com Colletotrichum gloeosporioides
title Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
spellingShingle Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
Maia, Fernanda Gonçalves Martins
Coffea arabica
response defense
Coffea arabica
Colletotrichum gloeosporioides
resposta de defesa
title_short Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
title_full Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
title_fullStr Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
title_full_unstemmed Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
title_sort Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
author Maia, Fernanda Gonçalves Martins
author_facet Maia, Fernanda Gonçalves Martins
Ogoshi, Claudio
Pierre, Rosana Oliveira
Vieira, Jucilayne Fernandes
Ribeiro junior, Pedro Martins
Abreu, Mario Sobral
author_role author
author2 Ogoshi, Claudio
Pierre, Rosana Oliveira
Vieira, Jucilayne Fernandes
Ribeiro junior, Pedro Martins
Abreu, Mario Sobral
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Maia, Fernanda Gonçalves Martins
Ogoshi, Claudio
Pierre, Rosana Oliveira
Vieira, Jucilayne Fernandes
Ribeiro junior, Pedro Martins
Abreu, Mario Sobral
dc.subject.por.fl_str_mv Coffea arabica
response defense
Coffea arabica
Colletotrichum gloeosporioides
resposta de defesa
topic Coffea arabica
response defense
Coffea arabica
Colletotrichum gloeosporioides
resposta de defesa
description The objective of this work was to study the effects of different isolates of Colletotrichum gloeosporioides, as topigment level (chlorophyll a, b and total), total soluble phenols and soluble lignin in coffee seedlings obtained by artificially inoculatedtissue culture. The experiment was carried out in randomized block design in a 2 x 3 factorial scheme (2 genetic materials), 3 isolates(an isolate from mango fruits and another one from coffee plants), with 5 replicates and control (plants without inoculation). Theplants were inoculated with a suspension of 2 x 106 conidia.ml-1. The conidial suspension was sprayed on previously injured leaves.Leaves of inoculated plants were collected at 3 and 7 days after inoculation, except for leaves on the lower part of the plants.Afterwards, samples were prepared for the evaluation of chlorophyll, total soluble phenols and soluble lignin. The results showedthat the presence of the pathogen in healthy and diseased plants significantly reduced the amount of chlorophyll. At 7 days afterinoculation of healthy and diseased coffee plants higher total soluble phenol and soluble lignin levels were observed in response to theattack by Colletotrichum gloeosporioides.
publishDate 2012
dc.date.none.fl_str_mv 2012-08-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://coffeescience.ufla.br/index.php/Coffeescience/article/view/248
url https://coffeescience.ufla.br/index.php/Coffeescience/article/view/248
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://coffeescience.ufla.br/index.php/Coffeescience/article/view/248/pdf
dc.rights.driver.fl_str_mv Copyright (c) 2012 Coffee Science - ISSN 1984-3909
https://creativecommons.org/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2012 Coffee Science - ISSN 1984-3909
https://creativecommons.org/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Editora UFLA
publisher.none.fl_str_mv Editora UFLA
dc.source.none.fl_str_mv Coffee Science - ISSN 1984-3909; Vol. 7 No. 2 (2012); 152-159
Coffee Science; Vol. 7 Núm. 2 (2012); 152-159
Coffee Science; v. 7 n. 2 (2012); 152-159
1984-3909
reponame:Coffee Science (Online)
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Coffee Science (Online)
collection Coffee Science (Online)
repository.name.fl_str_mv Coffee Science (Online) - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv coffeescience@dag.ufla.br||coffeescience@dag.ufla.br|| alvaro-cozadi@hotmail.com
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