Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content

Detalhes bibliográficos
Autor(a) principal: Silva, Luis Felipe Lima e
Data de Publicação: 2017
Outros Autores: Techio, Vânia Helena, Resende, Luciane Vilela, Braz, Guilherme Tomaz, Resende, Kátia Ferreira Marques de, Samartini, Carolina Queiroz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/31904
Resumo: The aim of this study was to perform chromosome counts and nuclear DNA quantification of the unconventional vegetables species: bertalha (Basella alba L.), vinagreira verde (Hibiscus sabdariffa L.), azedinha (Rumex acetosa L.), peixinho (Stachys byzantina K. Koch), and capuchinha (Tropaeolum majus L.). Metaphase chromosomes were obtained from the pre-treatment of root meristem with 8-hydroxyquinoline or colchicine and lides were prepared by the flame-drying technique and stained with 5% Giemsa. DNA quantification was performed by flow cytometry. Chromosome number and DNA content (pg) estimated for each species were: Rumex acetosa: 2n=2x=14 and 7.04 pg; Basella alba: 2n=2x=44 and 7.05 pg; Tropaeolum majus: 2n=2x=28 and 2.08 pg; Stachys byzantine: 2n=2x=30 and 1.54 pg, and Hibiscus sabdariffa: 2n=4x=72 and 5.12 pg.
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spelling Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA contentUnderutilized vegetablesGermplasmPlant breedingCytogenetic analysisLegumes subutilizadosGermoplasmaMelhoramento de plantasAnálise citogenéticaThe aim of this study was to perform chromosome counts and nuclear DNA quantification of the unconventional vegetables species: bertalha (Basella alba L.), vinagreira verde (Hibiscus sabdariffa L.), azedinha (Rumex acetosa L.), peixinho (Stachys byzantina K. Koch), and capuchinha (Tropaeolum majus L.). Metaphase chromosomes were obtained from the pre-treatment of root meristem with 8-hydroxyquinoline or colchicine and lides were prepared by the flame-drying technique and stained with 5% Giemsa. DNA quantification was performed by flow cytometry. Chromosome number and DNA content (pg) estimated for each species were: Rumex acetosa: 2n=2x=14 and 7.04 pg; Basella alba: 2n=2x=44 and 7.05 pg; Tropaeolum majus: 2n=2x=28 and 2.08 pg; Stachys byzantine: 2n=2x=30 and 1.54 pg, and Hibiscus sabdariffa: 2n=4x=72 and 5.12 pg.Crop Breeding and Applied Biotechnology2018-11-23T12:08:53Z2018-11-23T12:08:53Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSILVA, L. F. L. et al. Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, n. 4, p. 320-326, 2017.http://repositorio.ufla.br/jspui/handle/1/31904Crop Breeding and Applied Biotechnologyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessSilva, Luis Felipe Lima eTechio, Vânia HelenaResende, Luciane VilelaBraz, Guilherme TomazResende, Kátia Ferreira Marques deSamartini, Carolina Queirozeng2023-05-26T18:43:30Zoai:localhost:1/31904Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:43:30Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content
title Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content
spellingShingle Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content
Silva, Luis Felipe Lima e
Underutilized vegetables
Germplasm
Plant breeding
Cytogenetic analysis
Legumes subutilizados
Germoplasma
Melhoramento de plantas
Análise citogenética
title_short Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content
title_full Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content
title_fullStr Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content
title_full_unstemmed Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content
title_sort Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content
author Silva, Luis Felipe Lima e
author_facet Silva, Luis Felipe Lima e
Techio, Vânia Helena
Resende, Luciane Vilela
Braz, Guilherme Tomaz
Resende, Kátia Ferreira Marques de
Samartini, Carolina Queiroz
author_role author
author2 Techio, Vânia Helena
Resende, Luciane Vilela
Braz, Guilherme Tomaz
Resende, Kátia Ferreira Marques de
Samartini, Carolina Queiroz
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Luis Felipe Lima e
Techio, Vânia Helena
Resende, Luciane Vilela
Braz, Guilherme Tomaz
Resende, Kátia Ferreira Marques de
Samartini, Carolina Queiroz
dc.subject.por.fl_str_mv Underutilized vegetables
Germplasm
Plant breeding
Cytogenetic analysis
Legumes subutilizados
Germoplasma
Melhoramento de plantas
Análise citogenética
topic Underutilized vegetables
Germplasm
Plant breeding
Cytogenetic analysis
Legumes subutilizados
Germoplasma
Melhoramento de plantas
Análise citogenética
description The aim of this study was to perform chromosome counts and nuclear DNA quantification of the unconventional vegetables species: bertalha (Basella alba L.), vinagreira verde (Hibiscus sabdariffa L.), azedinha (Rumex acetosa L.), peixinho (Stachys byzantina K. Koch), and capuchinha (Tropaeolum majus L.). Metaphase chromosomes were obtained from the pre-treatment of root meristem with 8-hydroxyquinoline or colchicine and lides were prepared by the flame-drying technique and stained with 5% Giemsa. DNA quantification was performed by flow cytometry. Chromosome number and DNA content (pg) estimated for each species were: Rumex acetosa: 2n=2x=14 and 7.04 pg; Basella alba: 2n=2x=44 and 7.05 pg; Tropaeolum majus: 2n=2x=28 and 2.08 pg; Stachys byzantine: 2n=2x=30 and 1.54 pg, and Hibiscus sabdariffa: 2n=4x=72 and 5.12 pg.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018-11-23T12:08:53Z
2018-11-23T12:08:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, L. F. L. et al. Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, n. 4, p. 320-326, 2017.
http://repositorio.ufla.br/jspui/handle/1/31904
identifier_str_mv SILVA, L. F. L. et al. Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, n. 4, p. 320-326, 2017.
url http://repositorio.ufla.br/jspui/handle/1/31904
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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