Atividade acaricida do monoterpeno carvacrol sobre fêmeas adultas de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/15614 |
Resumo: | Resistant strains of Rhipicephalus (Boophilus) microplus to synthetic acaricides is increasing. Herd’s infestation by this tick is a problem in Brazil and in temperate countries. These arthropods cause huge economic losses to livestock due to their feeding habits that weaken the health and transmit diseases to their hosts. The phytotherapics, and especially carvacrol, have been shown to be an alternative in the control of this tick, since they have acaricidal and repellent effects, besides setting up a partially safe method, due to the rapid degradation and low contamination of the environment, the consumer of animal products and the host. Therefore, the objective of the present study was to determine the efficacy of carvacrol on adult R. microplus females by evaluating mortality and reproductive parameters. Bioassays were performed to determine the LC50 and to evaluate the reproductive parameters of the females, both from the Adult Immersion Test (AIT). For the determination of the LC50, eleven groups (n = 360) were separated in concentrations ranging from 0.099 to 1.304% and two groups served as negative control, one distilled water (100%) and the other 50% (v/v). To evaluate the reproductive parameters, the females were submitted to AIT in treatments containing 20% (n = 10), 40% (n = 10), 60% (n = 13), 80% (N n = 20) and 100% of LC50 (n = 20), and in control treatments of distilled water (100%) (n = 10) and ethyl alcohol 50% (v/v) (n = 10). The groups were maintained at 27 ± 1 ° C and humidity> 80 ± 10%. Egg weight, egg production index, pre-oviposition period and fecundity rate of the 80% and 100% LC50 groups presented a statistical difference (p<0.05) in the control groups. The LC50 found was 20.11 mg / mL and the control efficacy of this concentration was 64.67% compared to the ethyl alcohol control 50% (v/v). Thus, the results demonstrate that carvacrol has a significant acaricidal potential, and can contribute as another alternative in the control of R. microplus. |
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Atividade acaricida do monoterpeno carvacrol sobre fêmeas adultas de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)Acaricidal activity of monoterpene carvacrol on Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) adult femalesBovino - FitoterapiaCarrapato como transmissor de doençasÓleo essencialCattle – FithotherapyTicks as carriers of diseaseEssential oilFarmacologia e Terapêutica AnimalResistant strains of Rhipicephalus (Boophilus) microplus to synthetic acaricides is increasing. Herd’s infestation by this tick is a problem in Brazil and in temperate countries. These arthropods cause huge economic losses to livestock due to their feeding habits that weaken the health and transmit diseases to their hosts. The phytotherapics, and especially carvacrol, have been shown to be an alternative in the control of this tick, since they have acaricidal and repellent effects, besides setting up a partially safe method, due to the rapid degradation and low contamination of the environment, the consumer of animal products and the host. Therefore, the objective of the present study was to determine the efficacy of carvacrol on adult R. microplus females by evaluating mortality and reproductive parameters. Bioassays were performed to determine the LC50 and to evaluate the reproductive parameters of the females, both from the Adult Immersion Test (AIT). For the determination of the LC50, eleven groups (n = 360) were separated in concentrations ranging from 0.099 to 1.304% and two groups served as negative control, one distilled water (100%) and the other 50% (v/v). To evaluate the reproductive parameters, the females were submitted to AIT in treatments containing 20% (n = 10), 40% (n = 10), 60% (n = 13), 80% (N n = 20) and 100% of LC50 (n = 20), and in control treatments of distilled water (100%) (n = 10) and ethyl alcohol 50% (v/v) (n = 10). The groups were maintained at 27 ± 1 ° C and humidity> 80 ± 10%. Egg weight, egg production index, pre-oviposition period and fecundity rate of the 80% and 100% LC50 groups presented a statistical difference (p<0.05) in the control groups. The LC50 found was 20.11 mg / mL and the control efficacy of this concentration was 64.67% compared to the ethyl alcohol control 50% (v/v). Thus, the results demonstrate that carvacrol has a significant acaricidal potential, and can contribute as another alternative in the control of R. microplus.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)O desenvolvimento de resistência de cepas de Rhipicephalus (Boophilus) microplus a acaricidas sintéticos é crescente. A infestação dos rebanhos por esse carrapato é um problema no Brasil e em países de clima temperado. Este artrópode causa enormes perdas econômicas à pecuária em virtude de seu hábito alimentar que debilita a saúde e transmite doenças aos seus hospedeiros. Os fitoterápicos e, em especial o carvacrol, têm se revelado uma alternativa no controle desse carrapato, uma vez que apresentam efeitos acaricidas e repelentes, além de configurarem método parcialmente seguro, pela rápida degradação e baixa contaminação do ambiente, do consumidor de produtos de origem animal e do hospedeiro. Sendo assim, o objetivo do presente estudo foi determinar a eficácia do carvacrol sobre fêmeas adultas de R. microplus por meio da avaliação da mortalidade e de parâmetros reprodutivos. Foram realizados bioensaios para determinação da CL50 e avaliação dos parâmetros reprodutivos das fêmeas, ambos a partir do Teste de Imersão de Adultos (TIA). Para determinação da CL50 onze grupos (n = 360) foram separados em concentrações que variaram de 0,099 a 1,304% e dois grupos serviram de controle negativo, um a base de água destilada (100%) e outro de álcool etílico 50% (v/v). Para avaliação dos parâmetros reprodutivos, as fêmeas foram submetias ao TIA em tratamentos contendo 20% (n = 10), 40% (n = 10), 60% (n = 13), 80% (n = 16) e 100% da CL50 (n = 20), e em tratamentos controle de água destilada (100%) (n = 10) e outro de álcool etílico 50% (v/v) (n = 10). Os grupos foram mantidos a 27±1 °C e umidade>80±10 %. O peso da massa de ovos, o índice de produção de ovos, o período de pré-oviposição e a taxa de fecundidade dos grupos 80% e 100% da CL50 apresentaram diferença estatística (p<0,05) comparados aos grupos controle. A CL50 encontrada foi de 20,11 mg/mL e a eficácia de controle desta concentração foi de 61,10% quando comparada ao controle de água destilada. Sendo assim, os resultados demonstram que o carvacrol possui atividade acaricida sobre R. microplus.Universidade Federal de LavrasPrograma de Pós-Graduação em Ciências VeterináriasUFLAbrasilDepartamento de Medicina VeterináriaPeconick, Ana PaulaRemedio, Rafael NeodiniRemedio, Rafael NeodiniGuimarães, Antônio MarcosRocha, Christiane Maria Barcellos MagalhãesPereira Júnior, Amilcar Machado2017-11-01T16:32:41Z2017-11-01T16:32:41Z2017-11-012017-07-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfPEREIRA, JÚNIOR, A. M. Atividade acaricida do monoterpeno carvacrol sobre fêmeas adultas de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). 2017. 55 p. Dissertação (Mestrado em Ciências Veterinárias)-Universidade Federal de Lavras, Lavras, 2017.http://repositorio.ufla.br/jspui/handle/1/15614porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2023-05-09T19:23:29Zoai:localhost:1/15614Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-09T19:23:29Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Atividade acaricida do monoterpeno carvacrol sobre fêmeas adultas de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) Acaricidal activity of monoterpene carvacrol on Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) adult females |
title |
Atividade acaricida do monoterpeno carvacrol sobre fêmeas adultas de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
spellingShingle |
Atividade acaricida do monoterpeno carvacrol sobre fêmeas adultas de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) Pereira Júnior, Amilcar Machado Bovino - Fitoterapia Carrapato como transmissor de doenças Óleo essencial Cattle – Fithotherapy Ticks as carriers of disease Essential oil Farmacologia e Terapêutica Animal |
title_short |
Atividade acaricida do monoterpeno carvacrol sobre fêmeas adultas de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
title_full |
Atividade acaricida do monoterpeno carvacrol sobre fêmeas adultas de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
title_fullStr |
Atividade acaricida do monoterpeno carvacrol sobre fêmeas adultas de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
title_full_unstemmed |
Atividade acaricida do monoterpeno carvacrol sobre fêmeas adultas de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
title_sort |
Atividade acaricida do monoterpeno carvacrol sobre fêmeas adultas de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
author |
Pereira Júnior, Amilcar Machado |
author_facet |
Pereira Júnior, Amilcar Machado |
author_role |
author |
dc.contributor.none.fl_str_mv |
Peconick, Ana Paula Remedio, Rafael Neodini Remedio, Rafael Neodini Guimarães, Antônio Marcos Rocha, Christiane Maria Barcellos Magalhães |
dc.contributor.author.fl_str_mv |
Pereira Júnior, Amilcar Machado |
dc.subject.por.fl_str_mv |
Bovino - Fitoterapia Carrapato como transmissor de doenças Óleo essencial Cattle – Fithotherapy Ticks as carriers of disease Essential oil Farmacologia e Terapêutica Animal |
topic |
Bovino - Fitoterapia Carrapato como transmissor de doenças Óleo essencial Cattle – Fithotherapy Ticks as carriers of disease Essential oil Farmacologia e Terapêutica Animal |
description |
Resistant strains of Rhipicephalus (Boophilus) microplus to synthetic acaricides is increasing. Herd’s infestation by this tick is a problem in Brazil and in temperate countries. These arthropods cause huge economic losses to livestock due to their feeding habits that weaken the health and transmit diseases to their hosts. The phytotherapics, and especially carvacrol, have been shown to be an alternative in the control of this tick, since they have acaricidal and repellent effects, besides setting up a partially safe method, due to the rapid degradation and low contamination of the environment, the consumer of animal products and the host. Therefore, the objective of the present study was to determine the efficacy of carvacrol on adult R. microplus females by evaluating mortality and reproductive parameters. Bioassays were performed to determine the LC50 and to evaluate the reproductive parameters of the females, both from the Adult Immersion Test (AIT). For the determination of the LC50, eleven groups (n = 360) were separated in concentrations ranging from 0.099 to 1.304% and two groups served as negative control, one distilled water (100%) and the other 50% (v/v). To evaluate the reproductive parameters, the females were submitted to AIT in treatments containing 20% (n = 10), 40% (n = 10), 60% (n = 13), 80% (N n = 20) and 100% of LC50 (n = 20), and in control treatments of distilled water (100%) (n = 10) and ethyl alcohol 50% (v/v) (n = 10). The groups were maintained at 27 ± 1 ° C and humidity> 80 ± 10%. Egg weight, egg production index, pre-oviposition period and fecundity rate of the 80% and 100% LC50 groups presented a statistical difference (p<0.05) in the control groups. The LC50 found was 20.11 mg / mL and the control efficacy of this concentration was 64.67% compared to the ethyl alcohol control 50% (v/v). Thus, the results demonstrate that carvacrol has a significant acaricidal potential, and can contribute as another alternative in the control of R. microplus. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01T16:32:41Z 2017-11-01T16:32:41Z 2017-11-01 2017-07-31 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PEREIRA, JÚNIOR, A. M. Atividade acaricida do monoterpeno carvacrol sobre fêmeas adultas de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). 2017. 55 p. Dissertação (Mestrado em Ciências Veterinárias)-Universidade Federal de Lavras, Lavras, 2017. http://repositorio.ufla.br/jspui/handle/1/15614 |
identifier_str_mv |
PEREIRA, JÚNIOR, A. M. Atividade acaricida do monoterpeno carvacrol sobre fêmeas adultas de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). 2017. 55 p. Dissertação (Mestrado em Ciências Veterinárias)-Universidade Federal de Lavras, Lavras, 2017. |
url |
http://repositorio.ufla.br/jspui/handle/1/15614 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Ciências Veterinárias UFLA brasil Departamento de Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Ciências Veterinárias UFLA brasil Departamento de Medicina Veterinária |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
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UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835148467568640 |