Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium

Detalhes bibliográficos
Autor(a) principal: Gavilan, Natália Helena
Data de Publicação: 2018
Outros Autores: Furlan, Fernanda Cardoso, Zorz, Alex Zichner, Oliveira, Leandro Silva de, Campos, Wellington Ferreira, Brondani, Gilvano Ebling
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/33764
Resumo: Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving, 0.001% and 0.005% Cl. Moreover, the callus induction in the culture medium with 0.001% and 0.005% Cl was, respectively, 30% and 20% major than autoclaving sterilization. There was not significant (P<0.05) in the percentage of shoot induction among the sterilization preparations methods, and 65% of the explants survived in the presence of culture medium with 0.005% Cl. Histological analyses indicated that the Cl did not have any deleterious effects on morphogenic events. These results indicated that the chemical sterilization using 0.001% - 0.005% Cl controlled the fungal and bacterial multiplication in the culture medium and no affected the C. regium explants development, becoming it an alternative to autoclaving method.
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spelling Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regiumEsterilização química do meio de cultura para a multiplicação in vitro de Cochlospermum regiumAutoclavingCerrado biomeActive chlorineIn vitro cultivationAutoclavagemBioma cerradoCloro ativoCultivo in vitroCochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving, 0.001% and 0.005% Cl. Moreover, the callus induction in the culture medium with 0.001% and 0.005% Cl was, respectively, 30% and 20% major than autoclaving sterilization. There was not significant (P<0.05) in the percentage of shoot induction among the sterilization preparations methods, and 65% of the explants survived in the presence of culture medium with 0.005% Cl. Histological analyses indicated that the Cl did not have any deleterious effects on morphogenic events. These results indicated that the chemical sterilization using 0.001% - 0.005% Cl controlled the fungal and bacterial multiplication in the culture medium and no affected the C. regium explants development, becoming it an alternative to autoclaving method.As raízes de Cochlospermum regium são usadas na medicina popular, pois seu extrato apresenta diversas moléculas fitoquímicas, algumas com atividade antimicrobiana, que, consequentemente, expõe esta espécie ao risco de erosão genética. Assim, o objetivo deste estudo foi elaborar um protocolo de multiplicação in vitro para explantes de C. regium usando esterilização química do meio de cultura. Gemas apicais obtidas de plântulas de C. regium germinadas in vitro foram inoculadas em meio de cultivo MS suplementado com 0,05mg L-1 de ANA e 1mg L-1 de BAP e esterilizado pela adição do agente químico hipoclorito de sódio (NaOCl) nas concentrações de cloro ativo de 0,001%, 0,003% e 0,005%. O meio autoclavado foi utilizado como controle. Os resultados mostraram que a contaminação por bactérias aos 91 dias de cultivo foi significativamente (P<0,05) controlada pela autoclavagem, 0,001% e 0,005% de cloro ativo. Além disso, a indução de calos no meio de cultura com 0,001% e 0,005% de cloro ativo foi, respectivamente, 30% e 20% maior do que na esterilização por autoclavagem. Não houve diferença significativa (P<0,05) da porcentagem de indução de brotos entre os métodos de esterilização e 65% dos explantes sobreviveram na presença de 0,005% de cloro ativo. Análises histológicas indicaram que o cloro ativo não afetou os eventos morfogênicos. Os resultados indicaram que a esterilização química por meio do uso de 0,001% - 0,005% de cloro ativo auxiliou no controle da proliferação de bactérias e fungos no meio de cultura e não afetou o desenvolvimento dos explantes de C. regium, tornando-a uma alternativa em relação a autoclavagemUniversidade Federal de Santa Maria2019-04-24T12:27:20Z2019-04-24T12:27:20Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGAVILAN, N. H. et al. Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium. Ciência Rural, Santa Maria, v. 48, n. 9, 2018.http://repositorio.ufla.br/jspui/handle/1/33764Ciência Ruralreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessGavilan, Natália HelenaFurlan, Fernanda CardosoZorz, Alex ZichnerOliveira, Leandro Silva deCampos, Wellington FerreiraBrondani, Gilvano Eblingeng2019-04-24T12:27:21Zoai:localhost:1/33764Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-04-24T12:27:21Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium
Esterilização química do meio de cultura para a multiplicação in vitro de Cochlospermum regium
title Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium
spellingShingle Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium
Gavilan, Natália Helena
Autoclaving
Cerrado biome
Active chlorine
In vitro cultivation
Autoclavagem
Bioma cerrado
Cloro ativo
Cultivo in vitro
title_short Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium
title_full Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium
title_fullStr Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium
title_full_unstemmed Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium
title_sort Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium
author Gavilan, Natália Helena
author_facet Gavilan, Natália Helena
Furlan, Fernanda Cardoso
Zorz, Alex Zichner
Oliveira, Leandro Silva de
Campos, Wellington Ferreira
Brondani, Gilvano Ebling
author_role author
author2 Furlan, Fernanda Cardoso
Zorz, Alex Zichner
Oliveira, Leandro Silva de
Campos, Wellington Ferreira
Brondani, Gilvano Ebling
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Gavilan, Natália Helena
Furlan, Fernanda Cardoso
Zorz, Alex Zichner
Oliveira, Leandro Silva de
Campos, Wellington Ferreira
Brondani, Gilvano Ebling
dc.subject.por.fl_str_mv Autoclaving
Cerrado biome
Active chlorine
In vitro cultivation
Autoclavagem
Bioma cerrado
Cloro ativo
Cultivo in vitro
topic Autoclaving
Cerrado biome
Active chlorine
In vitro cultivation
Autoclavagem
Bioma cerrado
Cloro ativo
Cultivo in vitro
description Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving, 0.001% and 0.005% Cl. Moreover, the callus induction in the culture medium with 0.001% and 0.005% Cl was, respectively, 30% and 20% major than autoclaving sterilization. There was not significant (P<0.05) in the percentage of shoot induction among the sterilization preparations methods, and 65% of the explants survived in the presence of culture medium with 0.005% Cl. Histological analyses indicated that the Cl did not have any deleterious effects on morphogenic events. These results indicated that the chemical sterilization using 0.001% - 0.005% Cl controlled the fungal and bacterial multiplication in the culture medium and no affected the C. regium explants development, becoming it an alternative to autoclaving method.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019-04-24T12:27:20Z
2019-04-24T12:27:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv GAVILAN, N. H. et al. Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium. Ciência Rural, Santa Maria, v. 48, n. 9, 2018.
http://repositorio.ufla.br/jspui/handle/1/33764
identifier_str_mv GAVILAN, N. H. et al. Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium. Ciência Rural, Santa Maria, v. 48, n. 9, 2018.
url http://repositorio.ufla.br/jspui/handle/1/33764
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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