Chemical sterilization of culture medium for in vitro multiplication of cochlospermum regium
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-8478cr20170581 http://hdl.handle.net/11449/171386 |
Resumo: | Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving, 0.001% and 0.005% Cl. Moreover, the callus induction in the culture medium with 0.001% and 0.005% Cl was, respectively, 30% and 20% major than autoclaving sterilization. There was not significant (P<0.05) in the percentage of shoot induction among the sterilization preparations methods, and 65% of the explants survived in the presence of culture medium with 0.005% Cl. Histological analyses indicated that the Cl did not have any deleterious effects on morphogenic events. These results indicated that the chemical sterilization using 0.001% - 0.005% Cl controlled the fungal and bacterial multiplication in the culture medium and no affected the C. regium explants development, becoming it an alternative to autoclaving method. |
id |
UNSP_e1741f1bfb50a2164b6ded02a54fa7a2 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/171386 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Chemical sterilization of culture medium for in vitro multiplication of cochlospermum regiumEsterilização química do meio de cultura para a multiplicação in vitro de cochlospermum regiumActive chlorineAutoclavingCerrado biomeIn vitro cultivationCochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving, 0.001% and 0.005% Cl. Moreover, the callus induction in the culture medium with 0.001% and 0.005% Cl was, respectively, 30% and 20% major than autoclaving sterilization. There was not significant (P<0.05) in the percentage of shoot induction among the sterilization preparations methods, and 65% of the explants survived in the presence of culture medium with 0.005% Cl. Histological analyses indicated that the Cl did not have any deleterious effects on morphogenic events. These results indicated that the chemical sterilization using 0.001% - 0.005% Cl controlled the fungal and bacterial multiplication in the culture medium and no affected the C. regium explants development, becoming it an alternative to autoclaving method.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Instituto Chico Mendes de Conservação da BiodiversidadeUniversidade Federal de Mato Grosso (UFMT)Universidade Estadual Paulista (UNESP)Universidade Federal de Minas Gerais (UFMG)Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)Departamento de Ciências Florestais Universidade Federal de Lavras (UFLA)Universidade Estadual Paulista (UNESP)Universidade Federal de Mato Grosso (UFMT)Universidade Estadual Paulista (Unesp)Universidade Federal de Minas Gerais (UFMG)Universidade Federal de Viçosa (UFV)Universidade Federal de Lavras (UFLA)Gavilan, Natália Helena [UNESP]Furlan, Fernanda CardosoZorz, Alex Zichnerde Oliveira, Leandro SilvaCampos, Wellington FerreiraBrondani, Gilvano Ebling2018-12-11T16:55:06Z2018-12-11T16:55:06Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/0103-8478cr20170581Ciencia Rural, v. 48, n. 9, 2018.1678-45960103-8478http://hdl.handle.net/11449/17138610.1590/0103-8478cr20170581S0103-847820180009004002-s2.0-85052494241S0103-84782018000900400.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiencia Rural0,337info:eu-repo/semantics/openAccess2023-11-23T06:12:43Zoai:repositorio.unesp.br:11449/171386Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-23T06:12:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chemical sterilization of culture medium for in vitro multiplication of cochlospermum regium Esterilização química do meio de cultura para a multiplicação in vitro de cochlospermum regium |
title |
Chemical sterilization of culture medium for in vitro multiplication of cochlospermum regium |
spellingShingle |
Chemical sterilization of culture medium for in vitro multiplication of cochlospermum regium Gavilan, Natália Helena [UNESP] Active chlorine Autoclaving Cerrado biome In vitro cultivation |
title_short |
Chemical sterilization of culture medium for in vitro multiplication of cochlospermum regium |
title_full |
Chemical sterilization of culture medium for in vitro multiplication of cochlospermum regium |
title_fullStr |
Chemical sterilization of culture medium for in vitro multiplication of cochlospermum regium |
title_full_unstemmed |
Chemical sterilization of culture medium for in vitro multiplication of cochlospermum regium |
title_sort |
Chemical sterilization of culture medium for in vitro multiplication of cochlospermum regium |
author |
Gavilan, Natália Helena [UNESP] |
author_facet |
Gavilan, Natália Helena [UNESP] Furlan, Fernanda Cardoso Zorz, Alex Zichner de Oliveira, Leandro Silva Campos, Wellington Ferreira Brondani, Gilvano Ebling |
author_role |
author |
author2 |
Furlan, Fernanda Cardoso Zorz, Alex Zichner de Oliveira, Leandro Silva Campos, Wellington Ferreira Brondani, Gilvano Ebling |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Mato Grosso (UFMT) Universidade Estadual Paulista (Unesp) Universidade Federal de Minas Gerais (UFMG) Universidade Federal de Viçosa (UFV) Universidade Federal de Lavras (UFLA) |
dc.contributor.author.fl_str_mv |
Gavilan, Natália Helena [UNESP] Furlan, Fernanda Cardoso Zorz, Alex Zichner de Oliveira, Leandro Silva Campos, Wellington Ferreira Brondani, Gilvano Ebling |
dc.subject.por.fl_str_mv |
Active chlorine Autoclaving Cerrado biome In vitro cultivation |
topic |
Active chlorine Autoclaving Cerrado biome In vitro cultivation |
description |
Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving, 0.001% and 0.005% Cl. Moreover, the callus induction in the culture medium with 0.001% and 0.005% Cl was, respectively, 30% and 20% major than autoclaving sterilization. There was not significant (P<0.05) in the percentage of shoot induction among the sterilization preparations methods, and 65% of the explants survived in the presence of culture medium with 0.005% Cl. Histological analyses indicated that the Cl did not have any deleterious effects on morphogenic events. These results indicated that the chemical sterilization using 0.001% - 0.005% Cl controlled the fungal and bacterial multiplication in the culture medium and no affected the C. regium explants development, becoming it an alternative to autoclaving method. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:55:06Z 2018-12-11T16:55:06Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-8478cr20170581 Ciencia Rural, v. 48, n. 9, 2018. 1678-4596 0103-8478 http://hdl.handle.net/11449/171386 10.1590/0103-8478cr20170581 S0103-84782018000900400 2-s2.0-85052494241 S0103-84782018000900400.pdf |
url |
http://dx.doi.org/10.1590/0103-8478cr20170581 http://hdl.handle.net/11449/171386 |
identifier_str_mv |
Ciencia Rural, v. 48, n. 9, 2018. 1678-4596 0103-8478 10.1590/0103-8478cr20170581 S0103-84782018000900400 2-s2.0-85052494241 S0103-84782018000900400.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ciencia Rural 0,337 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046620092170240 |