Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/48494 |
Resumo: | Essential oils, also known as volatile oils, are substances produced through the secondary metabolism of plants. In this study, we determined the chemical composition and the in vitro and in vivo antifungal activity of the essential oils from four species of Eucalyptus, Eucalyptus citriodora, Eucalyptus camaldulensis, Eucalyptus grandis and Eucalyptus microcorys, against the Hemileia vastatrix fungus. The essential oils from these four species of Eucalyptus were extracted from their leaves by the hydrodistillation technique using a modified Clevenger apparatus. The chemical characterization was performed by gas chromatography coupled with a mass spectrometer detector and by gas chromatography using a flame ionization detector. The antifungal activities of the essential oils against H. vastatrix were studied by evaluating the percentage of spore germination using the microdilution test for in vitro assays. The curative and preventive effects were evaluated in in vivo tests. The principal constituents of the essential oil from E. citriodora were citronellal, citronellol and isopulegol, while E. camaldulensis produced 1,8-cineole, α-terpineol and α-pinene. 1,8-cineole, α-pinene and α-terpineol were obtained from E. grandis and 1,8-cineole, α-pinene and trans-pinocarveol were the principal components in the essential oil of E. microcorys. In vitro and in vivo antifungal activities against the fungus under study were observed for most of the essential oils, except the essential oil from E. microcorys, for which no preventive antifungal activity was observed. Only the curing of infection by the H. vastatrix fungus was observed with this oil. |
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Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rustCoffeeCoffee rustMicrobiological activityNatural productsOrganic agricultureÓleos essenciaisFerrugem do cafeeiroAtividade microbiológicaProdutos naturaisAgricultura orgânicaEssential oils, also known as volatile oils, are substances produced through the secondary metabolism of plants. In this study, we determined the chemical composition and the in vitro and in vivo antifungal activity of the essential oils from four species of Eucalyptus, Eucalyptus citriodora, Eucalyptus camaldulensis, Eucalyptus grandis and Eucalyptus microcorys, against the Hemileia vastatrix fungus. The essential oils from these four species of Eucalyptus were extracted from their leaves by the hydrodistillation technique using a modified Clevenger apparatus. The chemical characterization was performed by gas chromatography coupled with a mass spectrometer detector and by gas chromatography using a flame ionization detector. The antifungal activities of the essential oils against H. vastatrix were studied by evaluating the percentage of spore germination using the microdilution test for in vitro assays. The curative and preventive effects were evaluated in in vivo tests. The principal constituents of the essential oil from E. citriodora were citronellal, citronellol and isopulegol, while E. camaldulensis produced 1,8-cineole, α-terpineol and α-pinene. 1,8-cineole, α-pinene and α-terpineol were obtained from E. grandis and 1,8-cineole, α-pinene and trans-pinocarveol were the principal components in the essential oil of E. microcorys. In vitro and in vivo antifungal activities against the fungus under study were observed for most of the essential oils, except the essential oil from E. microcorys, for which no preventive antifungal activity was observed. Only the curing of infection by the H. vastatrix fungus was observed with this oil.Southern Cross Publishing2021-11-17T20:45:48Z2021-11-17T20:45:48Z2020-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCAETANO, A. R. S. et al. Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust. Australian Journal of Crop Science, [S. I.], v. 14, n. 9, p. 1379-1384, Sept. 2020. DOI: 10.21475/ajcs.20.14.09.p2249.http://repositorio.ufla.br/jspui/handle/1/48494Australian Journal of Crop Sciencereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessCaetano, Alex Rodrigues SilvaChalfoun, Sara MariaResende, Mario Lúcio VilelaAngélico, Caroline LimaSantiago, Wilder DouglasMagalhães, Maísa LamounierRezende, Danúbia Aparecida de Carvalho SelvatiSoares, Luana IsacNelson, David LeeCardoso, Maria das Graçaseng2021-11-17T20:46:35Zoai:localhost:1/48494Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-11-17T20:46:35Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust |
title |
Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust |
spellingShingle |
Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust Caetano, Alex Rodrigues Silva Coffee Coffee rust Microbiological activity Natural products Organic agriculture Óleos essenciais Ferrugem do cafeeiro Atividade microbiológica Produtos naturais Agricultura orgânica |
title_short |
Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust |
title_full |
Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust |
title_fullStr |
Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust |
title_full_unstemmed |
Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust |
title_sort |
Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust |
author |
Caetano, Alex Rodrigues Silva |
author_facet |
Caetano, Alex Rodrigues Silva Chalfoun, Sara Maria Resende, Mario Lúcio Vilela Angélico, Caroline Lima Santiago, Wilder Douglas Magalhães, Maísa Lamounier Rezende, Danúbia Aparecida de Carvalho Selvati Soares, Luana Isac Nelson, David Lee Cardoso, Maria das Graças |
author_role |
author |
author2 |
Chalfoun, Sara Maria Resende, Mario Lúcio Vilela Angélico, Caroline Lima Santiago, Wilder Douglas Magalhães, Maísa Lamounier Rezende, Danúbia Aparecida de Carvalho Selvati Soares, Luana Isac Nelson, David Lee Cardoso, Maria das Graças |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Caetano, Alex Rodrigues Silva Chalfoun, Sara Maria Resende, Mario Lúcio Vilela Angélico, Caroline Lima Santiago, Wilder Douglas Magalhães, Maísa Lamounier Rezende, Danúbia Aparecida de Carvalho Selvati Soares, Luana Isac Nelson, David Lee Cardoso, Maria das Graças |
dc.subject.por.fl_str_mv |
Coffee Coffee rust Microbiological activity Natural products Organic agriculture Óleos essenciais Ferrugem do cafeeiro Atividade microbiológica Produtos naturais Agricultura orgânica |
topic |
Coffee Coffee rust Microbiological activity Natural products Organic agriculture Óleos essenciais Ferrugem do cafeeiro Atividade microbiológica Produtos naturais Agricultura orgânica |
description |
Essential oils, also known as volatile oils, are substances produced through the secondary metabolism of plants. In this study, we determined the chemical composition and the in vitro and in vivo antifungal activity of the essential oils from four species of Eucalyptus, Eucalyptus citriodora, Eucalyptus camaldulensis, Eucalyptus grandis and Eucalyptus microcorys, against the Hemileia vastatrix fungus. The essential oils from these four species of Eucalyptus were extracted from their leaves by the hydrodistillation technique using a modified Clevenger apparatus. The chemical characterization was performed by gas chromatography coupled with a mass spectrometer detector and by gas chromatography using a flame ionization detector. The antifungal activities of the essential oils against H. vastatrix were studied by evaluating the percentage of spore germination using the microdilution test for in vitro assays. The curative and preventive effects were evaluated in in vivo tests. The principal constituents of the essential oil from E. citriodora were citronellal, citronellol and isopulegol, while E. camaldulensis produced 1,8-cineole, α-terpineol and α-pinene. 1,8-cineole, α-pinene and α-terpineol were obtained from E. grandis and 1,8-cineole, α-pinene and trans-pinocarveol were the principal components in the essential oil of E. microcorys. In vitro and in vivo antifungal activities against the fungus under study were observed for most of the essential oils, except the essential oil from E. microcorys, for which no preventive antifungal activity was observed. Only the curing of infection by the H. vastatrix fungus was observed with this oil. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09 2021-11-17T20:45:48Z 2021-11-17T20:45:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
CAETANO, A. R. S. et al. Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust. Australian Journal of Crop Science, [S. I.], v. 14, n. 9, p. 1379-1384, Sept. 2020. DOI: 10.21475/ajcs.20.14.09.p2249. http://repositorio.ufla.br/jspui/handle/1/48494 |
identifier_str_mv |
CAETANO, A. R. S. et al. Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust. Australian Journal of Crop Science, [S. I.], v. 14, n. 9, p. 1379-1384, Sept. 2020. DOI: 10.21475/ajcs.20.14.09.p2249. |
url |
http://repositorio.ufla.br/jspui/handle/1/48494 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Southern Cross Publishing |
publisher.none.fl_str_mv |
Southern Cross Publishing |
dc.source.none.fl_str_mv |
Australian Journal of Crop Science reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1784549977021743104 |