Protoplast production and isolation from Etlingera elatior
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/38870 |
Resumo: | The technique of hybridization using plant protoplasts is widely used in plant breeding programs. The purpose of our study is to further characterize the process of protoplast isolation from the ornamental species Etlingera elatior (Jack) R. M. Smith. Protoplasts were isolated from different tissues: in vitro leaves, in vitro pseudostem, and leaves from plants cultivated hydroponically. We tested six enzymatic combinations, four incubation time periods, the rotary system (40 rpm) or steady in the dark, and three concentrations of mannitol (0.5, 0.6 and 0.7 M). The diameter and viability of obtained protoplasts were evaluated. The best source of explants used for protoplast isolation was the in vitro leaves, which yielded 22x105 protoplasts g-1 of fresh matter. The optimal incubation period was 15 hours. The in vitro leaves presented a greater viability (96%) and larger protoplasts (36.7 μm diameter). Greater yields were obtained using a rotatory system with protoplasts incubated in the dark. The best enzymatic combination was 3% Cellulase “Onozuca” R-10 + 2% Meicelase + 1% Driselase + 1% Dextran + 5 mM MES, followed by the addition of 0.6 M mannito. |
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Protoplast production and isolation from Etlingera elatiorProdução e isolamento de protoplasto de Etlingera elatiorFDAOrnamental plantEnzymatic combinationsIncubation periodFluorescein diacetatePlanta ornamentalCombinações enzimáticasPeríodo de incubaçãoThe technique of hybridization using plant protoplasts is widely used in plant breeding programs. The purpose of our study is to further characterize the process of protoplast isolation from the ornamental species Etlingera elatior (Jack) R. M. Smith. Protoplasts were isolated from different tissues: in vitro leaves, in vitro pseudostem, and leaves from plants cultivated hydroponically. We tested six enzymatic combinations, four incubation time periods, the rotary system (40 rpm) or steady in the dark, and three concentrations of mannitol (0.5, 0.6 and 0.7 M). The diameter and viability of obtained protoplasts were evaluated. The best source of explants used for protoplast isolation was the in vitro leaves, which yielded 22x105 protoplasts g-1 of fresh matter. The optimal incubation period was 15 hours. The in vitro leaves presented a greater viability (96%) and larger protoplasts (36.7 μm diameter). Greater yields were obtained using a rotatory system with protoplasts incubated in the dark. The best enzymatic combination was 3% Cellulase “Onozuca” R-10 + 2% Meicelase + 1% Driselase + 1% Dextran + 5 mM MES, followed by the addition of 0.6 M mannito.Com o objetivo de realizar hibridações que auxiliam em programas de melhoramento genético de flores ornamentais, protoplastos foram isolados a partir de diferentes tecidos (folhas in vitro, pseudocaules in vitro e folhas em sistema hidropônico) de Etlnigera elatior (Jack) R. M. Smith. Foram testados seis diferentes combinações enzimáticas, quatro períodos de incubação, sistema rotatório (40 rpm) ou estacionário no escuro, concentrações de manitol (0,5; 0,6 e 0,7 M), o diâmetro e a viabilidade dos protoplastos isolados. A melhor fonte de explante utilizado no isolamento de protoplastos foi folha in vitro, com rendimento de 22 x10 5 protoplastos g-1 MF. O melhor tempo de incubação foi 15 horas, pois períodos superiores a este causavam diminuição no rendimento e viabilidade dos protoplastos. Protoplastos de folhas in vitro apresentaram viabilidade de 96% e diâmetro de 36,7 μm. Maiores rendimentos foram alcançados em sistema rotatório e no escuro. A melhor combinação enzimática utilizada no atual trabalho foi a 3% Cellulase “Onozuka” R-10 + 2% Meicelase + 1% Driselase + 1% Dextran + 5 mM MES. A melhor concentração de manitol foi de 0,6 M.Editora da Universidade Estadual de Maringá (EDUEM)2020-02-05T21:36:23Z2020-02-05T21:36:23Z2012-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSILVA JÚNIOR, J. M. da et al. Protoplast production and isolation from Etlingera elatior. Acta Scientiarum. Agronomy, Maringá, v. 34, n. 1, p. 45-60, Jan./Mar. 2012. DOI: 10.1590/S1807-86212012000100007.http://repositorio.ufla.br/jspui/handle/1/38870Acta Scientiarum. Agronomyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessSilva Júnior, Jessé Marques daPaiva, RenatoCampos, Ana Carolina Atala LombeloRodrigues, MarceloCarvalho, Milene Alves de FigueiredoOtoni, Wagner Camposeng2020-02-05T21:36:23Zoai:localhost:1/38870Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-02-05T21:36:23Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Protoplast production and isolation from Etlingera elatior Produção e isolamento de protoplasto de Etlingera elatior |
title |
Protoplast production and isolation from Etlingera elatior |
spellingShingle |
Protoplast production and isolation from Etlingera elatior Silva Júnior, Jessé Marques da FDA Ornamental plant Enzymatic combinations Incubation period Fluorescein diacetate Planta ornamental Combinações enzimáticas Período de incubação |
title_short |
Protoplast production and isolation from Etlingera elatior |
title_full |
Protoplast production and isolation from Etlingera elatior |
title_fullStr |
Protoplast production and isolation from Etlingera elatior |
title_full_unstemmed |
Protoplast production and isolation from Etlingera elatior |
title_sort |
Protoplast production and isolation from Etlingera elatior |
author |
Silva Júnior, Jessé Marques da |
author_facet |
Silva Júnior, Jessé Marques da Paiva, Renato Campos, Ana Carolina Atala Lombelo Rodrigues, Marcelo Carvalho, Milene Alves de Figueiredo Otoni, Wagner Campos |
author_role |
author |
author2 |
Paiva, Renato Campos, Ana Carolina Atala Lombelo Rodrigues, Marcelo Carvalho, Milene Alves de Figueiredo Otoni, Wagner Campos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva Júnior, Jessé Marques da Paiva, Renato Campos, Ana Carolina Atala Lombelo Rodrigues, Marcelo Carvalho, Milene Alves de Figueiredo Otoni, Wagner Campos |
dc.subject.por.fl_str_mv |
FDA Ornamental plant Enzymatic combinations Incubation period Fluorescein diacetate Planta ornamental Combinações enzimáticas Período de incubação |
topic |
FDA Ornamental plant Enzymatic combinations Incubation period Fluorescein diacetate Planta ornamental Combinações enzimáticas Período de incubação |
description |
The technique of hybridization using plant protoplasts is widely used in plant breeding programs. The purpose of our study is to further characterize the process of protoplast isolation from the ornamental species Etlingera elatior (Jack) R. M. Smith. Protoplasts were isolated from different tissues: in vitro leaves, in vitro pseudostem, and leaves from plants cultivated hydroponically. We tested six enzymatic combinations, four incubation time periods, the rotary system (40 rpm) or steady in the dark, and three concentrations of mannitol (0.5, 0.6 and 0.7 M). The diameter and viability of obtained protoplasts were evaluated. The best source of explants used for protoplast isolation was the in vitro leaves, which yielded 22x105 protoplasts g-1 of fresh matter. The optimal incubation period was 15 hours. The in vitro leaves presented a greater viability (96%) and larger protoplasts (36.7 μm diameter). Greater yields were obtained using a rotatory system with protoplasts incubated in the dark. The best enzymatic combination was 3% Cellulase “Onozuca” R-10 + 2% Meicelase + 1% Driselase + 1% Dextran + 5 mM MES, followed by the addition of 0.6 M mannito. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-03 2020-02-05T21:36:23Z 2020-02-05T21:36:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SILVA JÚNIOR, J. M. da et al. Protoplast production and isolation from Etlingera elatior. Acta Scientiarum. Agronomy, Maringá, v. 34, n. 1, p. 45-60, Jan./Mar. 2012. DOI: 10.1590/S1807-86212012000100007. http://repositorio.ufla.br/jspui/handle/1/38870 |
identifier_str_mv |
SILVA JÚNIOR, J. M. da et al. Protoplast production and isolation from Etlingera elatior. Acta Scientiarum. Agronomy, Maringá, v. 34, n. 1, p. 45-60, Jan./Mar. 2012. DOI: 10.1590/S1807-86212012000100007. |
url |
http://repositorio.ufla.br/jspui/handle/1/38870 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá (EDUEM) |
publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá (EDUEM) |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835126622584832 |