Microplants production of Eucalyptus cloeziana from indirect organogenesis

Detalhes bibliográficos
Autor(a) principal: Zorz, Alex Zichner
Data de Publicação: 2020
Outros Autores: Faria, Júlio Cézar Tannure, Souza, Denys Matheus Santana Costa, Gonçalves, Douglas Santos, Oliveira, Leandro Silva de, Silva, André Luís Lopes da, Campos, Wellington Ferreira, Brondani, Gilvano Ebling
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/46911
Resumo: Few studies have focused on the in vitro multiplication methods for Eucalyptus cloeziana. In this work we developed an indirect organogenesis protocol to obtain micropropagated plants. The interactions of plant growth regulators in juvenile tissues were evaluated. Hypocotyl, cotyledon and root tissues obtained from in vitro germinated seedlings were subjected to combinations of α-naphthaleneacetic acid (NAA), thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D). In vitro callogenesis, adventitious bud induction, shoot elongation, and ex vitro survival and rooting were evaluated. Callogenic structures were observed in all evaluated tissues. Morphogenic characteristics related to the meristematic center was observed. The tissues that presented the callus mass were subcultured in a regeneration culture medium supplemented with 1.0 mg L-1 of 6-benzylaminopurine (BAP). Only hypocotyl and cotyledon induced adventitious bud and shoot elongation. Hypocotyl subjected to 2.0 mg L-1 TDZ had the highest number of elongated shoots. The two tissues (i.e., hypocotyl and cotyledon) combined with thidiazuron were characterized by the ex vitro survival of microcuttings and by adventitious rooting. Results confirmed tissue culture efficiency for microplants production by indirect organogenesis from hypocotyl and cotyledon cultivated with TDZ, and its implementation can be an alternative for forest tree breeding programs of E. cloeziana.
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spelling Microplants production of Eucalyptus cloeziana from indirect organogenesisProducción de microplantas de Eucalyptus cloeziana por organogénesis indirectaAdventitious rootingCallogenesisIndirect regenerationAdventitious budEnraizamento adventícioCalogêneseOrganogênese indiretaGema adventíciaFew studies have focused on the in vitro multiplication methods for Eucalyptus cloeziana. In this work we developed an indirect organogenesis protocol to obtain micropropagated plants. The interactions of plant growth regulators in juvenile tissues were evaluated. Hypocotyl, cotyledon and root tissues obtained from in vitro germinated seedlings were subjected to combinations of α-naphthaleneacetic acid (NAA), thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D). In vitro callogenesis, adventitious bud induction, shoot elongation, and ex vitro survival and rooting were evaluated. Callogenic structures were observed in all evaluated tissues. Morphogenic characteristics related to the meristematic center was observed. The tissues that presented the callus mass were subcultured in a regeneration culture medium supplemented with 1.0 mg L-1 of 6-benzylaminopurine (BAP). Only hypocotyl and cotyledon induced adventitious bud and shoot elongation. Hypocotyl subjected to 2.0 mg L-1 TDZ had the highest number of elongated shoots. The two tissues (i.e., hypocotyl and cotyledon) combined with thidiazuron were characterized by the ex vitro survival of microcuttings and by adventitious rooting. Results confirmed tissue culture efficiency for microplants production by indirect organogenesis from hypocotyl and cotyledon cultivated with TDZ, and its implementation can be an alternative for forest tree breeding programs of E. cloeziana.Resumen: Pocos estudios se han centrado en los métodos de multiplicación in vitro para Eucalyptus cloeziana. En este trabajo se desarrolló un protocolo de organogénesis indirecta para obtener plantas micropropagadas. Se evaluaron las interacciones de los reguladores del crecimiento en tejidos juveniles. Los tejidos del hipocótilo, cotiledón y raíces obtenidos de plántulas germinadas in vitro se sometieron a combinaciones de ácido α-naftaleneacético (ANA), tidiazurón (TDZ) y ácido 2,4-diclorofenoxiacético (2,4-D). Se evaluaron la callogénesis in vitro, la inducción adventicia de yemas, el alargamiento de brotes, y la sobrevivencia y enraizamiento ex vitro. Se observaron estructuras calogénicas en todos los tejidos evaluados. Se observaron características morfogénicas relacionadas con la formación de centros meristemáticos. Los tejidos que presentaron la masa del callo fueron subcultivados en medio de cultivo de regeneración suplementado con 1,0 mg L-1 de 6-bencilaminopurina (BAP), y solo los tejidos del hipocótilo y cotiledón formaron yemas adventicias y brotes alargados. Los tejidos del hipocótilo sometidos a 2,0 mg L-1 de tidiazuron obtuvieron mayor número de brotes alargados. Los dos tejidos (hipocótilo y cotiledón) combinados con TDZ se caracterizaron por sobrevivencia y el enraizamiento adventicio ex vitro de las microestacas. Los resultados confirmaron la eficiencia del cultivo de tejidos para la producción de microplantas por organogénesis indirecta a partir de tejidos del hipocótilo y cotiledón cultivados con TDZ y su implementación puede ser una alternativa para los programas de mejoramiento forestal de E. cloeziana.Universidad Austral de Chile, Facultad de Ciencias Forestales2021-08-20T19:04:32Z2021-08-20T19:04:32Z2020-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfZORZ, A. Z. et al. Microplants production of Eucalyptus cloeziana from indirect organogenesis. Bosque, Valdivia, v. 41, n. 2, p. 113-124, 2020. DOI: 10.4067/S0717-92002020000200113.http://repositorio.ufla.br/jspui/handle/1/46911Bosque (Valdivia)reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessZorz, Alex ZichnerFaria, Júlio Cézar TannureSouza, Denys Matheus Santana CostaGonçalves, Douglas SantosOliveira, Leandro Silva deSilva, André Luís Lopes daCampos, Wellington FerreiraBrondani, Gilvano Eblingeng2021-08-20T19:05:14Zoai:localhost:1/46911Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-08-20T19:05:14Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Microplants production of Eucalyptus cloeziana from indirect organogenesis
Producción de microplantas de Eucalyptus cloeziana por organogénesis indirecta
title Microplants production of Eucalyptus cloeziana from indirect organogenesis
spellingShingle Microplants production of Eucalyptus cloeziana from indirect organogenesis
Zorz, Alex Zichner
Adventitious rooting
Callogenesis
Indirect regeneration
Adventitious bud
Enraizamento adventício
Calogênese
Organogênese indireta
Gema adventícia
title_short Microplants production of Eucalyptus cloeziana from indirect organogenesis
title_full Microplants production of Eucalyptus cloeziana from indirect organogenesis
title_fullStr Microplants production of Eucalyptus cloeziana from indirect organogenesis
title_full_unstemmed Microplants production of Eucalyptus cloeziana from indirect organogenesis
title_sort Microplants production of Eucalyptus cloeziana from indirect organogenesis
author Zorz, Alex Zichner
author_facet Zorz, Alex Zichner
Faria, Júlio Cézar Tannure
Souza, Denys Matheus Santana Costa
Gonçalves, Douglas Santos
Oliveira, Leandro Silva de
Silva, André Luís Lopes da
Campos, Wellington Ferreira
Brondani, Gilvano Ebling
author_role author
author2 Faria, Júlio Cézar Tannure
Souza, Denys Matheus Santana Costa
Gonçalves, Douglas Santos
Oliveira, Leandro Silva de
Silva, André Luís Lopes da
Campos, Wellington Ferreira
Brondani, Gilvano Ebling
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Zorz, Alex Zichner
Faria, Júlio Cézar Tannure
Souza, Denys Matheus Santana Costa
Gonçalves, Douglas Santos
Oliveira, Leandro Silva de
Silva, André Luís Lopes da
Campos, Wellington Ferreira
Brondani, Gilvano Ebling
dc.subject.por.fl_str_mv Adventitious rooting
Callogenesis
Indirect regeneration
Adventitious bud
Enraizamento adventício
Calogênese
Organogênese indireta
Gema adventícia
topic Adventitious rooting
Callogenesis
Indirect regeneration
Adventitious bud
Enraizamento adventício
Calogênese
Organogênese indireta
Gema adventícia
description Few studies have focused on the in vitro multiplication methods for Eucalyptus cloeziana. In this work we developed an indirect organogenesis protocol to obtain micropropagated plants. The interactions of plant growth regulators in juvenile tissues were evaluated. Hypocotyl, cotyledon and root tissues obtained from in vitro germinated seedlings were subjected to combinations of α-naphthaleneacetic acid (NAA), thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D). In vitro callogenesis, adventitious bud induction, shoot elongation, and ex vitro survival and rooting were evaluated. Callogenic structures were observed in all evaluated tissues. Morphogenic characteristics related to the meristematic center was observed. The tissues that presented the callus mass were subcultured in a regeneration culture medium supplemented with 1.0 mg L-1 of 6-benzylaminopurine (BAP). Only hypocotyl and cotyledon induced adventitious bud and shoot elongation. Hypocotyl subjected to 2.0 mg L-1 TDZ had the highest number of elongated shoots. The two tissues (i.e., hypocotyl and cotyledon) combined with thidiazuron were characterized by the ex vitro survival of microcuttings and by adventitious rooting. Results confirmed tissue culture efficiency for microplants production by indirect organogenesis from hypocotyl and cotyledon cultivated with TDZ, and its implementation can be an alternative for forest tree breeding programs of E. cloeziana.
publishDate 2020
dc.date.none.fl_str_mv 2020-08
2021-08-20T19:04:32Z
2021-08-20T19:04:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv ZORZ, A. Z. et al. Microplants production of Eucalyptus cloeziana from indirect organogenesis. Bosque, Valdivia, v. 41, n. 2, p. 113-124, 2020. DOI: 10.4067/S0717-92002020000200113.
http://repositorio.ufla.br/jspui/handle/1/46911
identifier_str_mv ZORZ, A. Z. et al. Microplants production of Eucalyptus cloeziana from indirect organogenesis. Bosque, Valdivia, v. 41, n. 2, p. 113-124, 2020. DOI: 10.4067/S0717-92002020000200113.
url http://repositorio.ufla.br/jspui/handle/1/46911
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Austral de Chile, Facultad de Ciencias Forestales
publisher.none.fl_str_mv Universidad Austral de Chile, Facultad de Ciencias Forestales
dc.source.none.fl_str_mv Bosque (Valdivia)
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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