In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia

Detalhes bibliográficos
Autor(a) principal: Almeida,Weliton Antonio Bastos de
Data de Publicação: 2002
Outros Autores: Mourão Filho,Francisco de Assis Alves, Mendes,Beatriz Madalena Januzzi, Rodriguez,Adriana Pinheiro Martinelli
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100004
Resumo: Exogenous genes can be introduced in plants by genetic transformation techniques. However, an efficient tissue culture system with high rates of plant recovery is necessary for gene introduction. This work aimed to define organogenesis and plant regeneration protocols for sweet orange varieties Natal, Valencia and Hamlin (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck) which can be used in plant transformation experiments. Seeds of which teguments were removed, were germinated in vitro and maintained in the dark for three weeks, followed by one week at 16-h photoperiod (40 µmol m-2 s-1) and 27 ± 2°C. Organogenesis induction was done by introducing epicotyl segments in MT medium with 25 g L-1 sucrose and different BAP concentrations. After adventitious bud growth, the shoots were transferred to MT medium with either NAA or IBA (1 mg L-1), or absence of auxin, for rooting. The best results were obtained with 1 mg L-1 BAP for bud induction and 1 mg L-1 IBA for rooting for all three sweet orange cultivars. The use of 0.5-2.5 mg L-1 BAP, followed by 1 mg L-1 IBA were the best growth regulator combinations for bud induction and rooting, respectively, for 'Rangpur' lime. The protocols presented in this work are suitable for associations with genetic transformation experiments for these cultivars.
id USP-18_e2ad661042310011416741da6d95fb5d
oai_identifier_str oai:scielo:S0103-90162002000100004
network_acronym_str USP-18
network_name_str Scientia Agrícola (Online)
repository_id_str
spelling In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limoniaadventitious budrootinggenetic transformationExogenous genes can be introduced in plants by genetic transformation techniques. However, an efficient tissue culture system with high rates of plant recovery is necessary for gene introduction. This work aimed to define organogenesis and plant regeneration protocols for sweet orange varieties Natal, Valencia and Hamlin (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck) which can be used in plant transformation experiments. Seeds of which teguments were removed, were germinated in vitro and maintained in the dark for three weeks, followed by one week at 16-h photoperiod (40 µmol m-2 s-1) and 27 ± 2°C. Organogenesis induction was done by introducing epicotyl segments in MT medium with 25 g L-1 sucrose and different BAP concentrations. After adventitious bud growth, the shoots were transferred to MT medium with either NAA or IBA (1 mg L-1), or absence of auxin, for rooting. The best results were obtained with 1 mg L-1 BAP for bud induction and 1 mg L-1 IBA for rooting for all three sweet orange cultivars. The use of 0.5-2.5 mg L-1 BAP, followed by 1 mg L-1 IBA were the best growth regulator combinations for bud induction and rooting, respectively, for 'Rangpur' lime. The protocols presented in this work are suitable for associations with genetic transformation experiments for these cultivars.Escola Superior de Agricultura "Luiz de Queiroz"2002-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100004Scientia Agricola v.59 n.1 2002reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162002000100004info:eu-repo/semantics/openAccessAlmeida,Weliton Antonio Bastos deMourão Filho,Francisco de Assis AlvesMendes,Beatriz Madalena JanuzziRodriguez,Adriana Pinheiro Martinellieng2002-09-27T00:00:00Zoai:scielo:S0103-90162002000100004Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2002-09-27T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia
title In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia
spellingShingle In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia
Almeida,Weliton Antonio Bastos de
adventitious bud
rooting
genetic transformation
title_short In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia
title_full In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia
title_fullStr In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia
title_full_unstemmed In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia
title_sort In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia
author Almeida,Weliton Antonio Bastos de
author_facet Almeida,Weliton Antonio Bastos de
Mourão Filho,Francisco de Assis Alves
Mendes,Beatriz Madalena Januzzi
Rodriguez,Adriana Pinheiro Martinelli
author_role author
author2 Mourão Filho,Francisco de Assis Alves
Mendes,Beatriz Madalena Januzzi
Rodriguez,Adriana Pinheiro Martinelli
author2_role author
author
author
dc.contributor.author.fl_str_mv Almeida,Weliton Antonio Bastos de
Mourão Filho,Francisco de Assis Alves
Mendes,Beatriz Madalena Januzzi
Rodriguez,Adriana Pinheiro Martinelli
dc.subject.por.fl_str_mv adventitious bud
rooting
genetic transformation
topic adventitious bud
rooting
genetic transformation
description Exogenous genes can be introduced in plants by genetic transformation techniques. However, an efficient tissue culture system with high rates of plant recovery is necessary for gene introduction. This work aimed to define organogenesis and plant regeneration protocols for sweet orange varieties Natal, Valencia and Hamlin (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck) which can be used in plant transformation experiments. Seeds of which teguments were removed, were germinated in vitro and maintained in the dark for three weeks, followed by one week at 16-h photoperiod (40 µmol m-2 s-1) and 27 ± 2°C. Organogenesis induction was done by introducing epicotyl segments in MT medium with 25 g L-1 sucrose and different BAP concentrations. After adventitious bud growth, the shoots were transferred to MT medium with either NAA or IBA (1 mg L-1), or absence of auxin, for rooting. The best results were obtained with 1 mg L-1 BAP for bud induction and 1 mg L-1 IBA for rooting for all three sweet orange cultivars. The use of 0.5-2.5 mg L-1 BAP, followed by 1 mg L-1 IBA were the best growth regulator combinations for bud induction and rooting, respectively, for 'Rangpur' lime. The protocols presented in this work are suitable for associations with genetic transformation experiments for these cultivars.
publishDate 2002
dc.date.none.fl_str_mv 2002-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-90162002000100004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.59 n.1 2002
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
_version_ 1748936458297671680