In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100004 |
Resumo: | Exogenous genes can be introduced in plants by genetic transformation techniques. However, an efficient tissue culture system with high rates of plant recovery is necessary for gene introduction. This work aimed to define organogenesis and plant regeneration protocols for sweet orange varieties Natal, Valencia and Hamlin (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck) which can be used in plant transformation experiments. Seeds of which teguments were removed, were germinated in vitro and maintained in the dark for three weeks, followed by one week at 16-h photoperiod (40 µmol m-2 s-1) and 27 ± 2°C. Organogenesis induction was done by introducing epicotyl segments in MT medium with 25 g L-1 sucrose and different BAP concentrations. After adventitious bud growth, the shoots were transferred to MT medium with either NAA or IBA (1 mg L-1), or absence of auxin, for rooting. The best results were obtained with 1 mg L-1 BAP for bud induction and 1 mg L-1 IBA for rooting for all three sweet orange cultivars. The use of 0.5-2.5 mg L-1 BAP, followed by 1 mg L-1 IBA were the best growth regulator combinations for bud induction and rooting, respectively, for 'Rangpur' lime. The protocols presented in this work are suitable for associations with genetic transformation experiments for these cultivars. |
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In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limoniaadventitious budrootinggenetic transformationExogenous genes can be introduced in plants by genetic transformation techniques. However, an efficient tissue culture system with high rates of plant recovery is necessary for gene introduction. This work aimed to define organogenesis and plant regeneration protocols for sweet orange varieties Natal, Valencia and Hamlin (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck) which can be used in plant transformation experiments. Seeds of which teguments were removed, were germinated in vitro and maintained in the dark for three weeks, followed by one week at 16-h photoperiod (40 µmol m-2 s-1) and 27 ± 2°C. Organogenesis induction was done by introducing epicotyl segments in MT medium with 25 g L-1 sucrose and different BAP concentrations. After adventitious bud growth, the shoots were transferred to MT medium with either NAA or IBA (1 mg L-1), or absence of auxin, for rooting. The best results were obtained with 1 mg L-1 BAP for bud induction and 1 mg L-1 IBA for rooting for all three sweet orange cultivars. The use of 0.5-2.5 mg L-1 BAP, followed by 1 mg L-1 IBA were the best growth regulator combinations for bud induction and rooting, respectively, for 'Rangpur' lime. The protocols presented in this work are suitable for associations with genetic transformation experiments for these cultivars.Escola Superior de Agricultura "Luiz de Queiroz"2002-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100004Scientia Agricola v.59 n.1 2002reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162002000100004info:eu-repo/semantics/openAccessAlmeida,Weliton Antonio Bastos deMourão Filho,Francisco de Assis AlvesMendes,Beatriz Madalena JanuzziRodriguez,Adriana Pinheiro Martinellieng2002-09-27T00:00:00Zoai:scielo:S0103-90162002000100004Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2002-09-27T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia |
title |
In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia |
spellingShingle |
In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia Almeida,Weliton Antonio Bastos de adventitious bud rooting genetic transformation |
title_short |
In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia |
title_full |
In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia |
title_fullStr |
In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia |
title_full_unstemmed |
In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia |
title_sort |
In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia |
author |
Almeida,Weliton Antonio Bastos de |
author_facet |
Almeida,Weliton Antonio Bastos de Mourão Filho,Francisco de Assis Alves Mendes,Beatriz Madalena Januzzi Rodriguez,Adriana Pinheiro Martinelli |
author_role |
author |
author2 |
Mourão Filho,Francisco de Assis Alves Mendes,Beatriz Madalena Januzzi Rodriguez,Adriana Pinheiro Martinelli |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Almeida,Weliton Antonio Bastos de Mourão Filho,Francisco de Assis Alves Mendes,Beatriz Madalena Januzzi Rodriguez,Adriana Pinheiro Martinelli |
dc.subject.por.fl_str_mv |
adventitious bud rooting genetic transformation |
topic |
adventitious bud rooting genetic transformation |
description |
Exogenous genes can be introduced in plants by genetic transformation techniques. However, an efficient tissue culture system with high rates of plant recovery is necessary for gene introduction. This work aimed to define organogenesis and plant regeneration protocols for sweet orange varieties Natal, Valencia and Hamlin (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck) which can be used in plant transformation experiments. Seeds of which teguments were removed, were germinated in vitro and maintained in the dark for three weeks, followed by one week at 16-h photoperiod (40 µmol m-2 s-1) and 27 ± 2°C. Organogenesis induction was done by introducing epicotyl segments in MT medium with 25 g L-1 sucrose and different BAP concentrations. After adventitious bud growth, the shoots were transferred to MT medium with either NAA or IBA (1 mg L-1), or absence of auxin, for rooting. The best results were obtained with 1 mg L-1 BAP for bud induction and 1 mg L-1 IBA for rooting for all three sweet orange cultivars. The use of 0.5-2.5 mg L-1 BAP, followed by 1 mg L-1 IBA were the best growth regulator combinations for bud induction and rooting, respectively, for 'Rangpur' lime. The protocols presented in this work are suitable for associations with genetic transformation experiments for these cultivars. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90162002000100004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.59 n.1 2002 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936458297671680 |