In vitro establishment and multiplication of genotypes of Eucalyptus dunnii Maiden

Detalhes bibliográficos
Autor(a) principal: Navroski, Marcio Carlos
Data de Publicação: 2016
Outros Autores: Reiniger, Lia Rejane Silveira, Araújo, Maristela Machado, Curti, Aline Ritter, Pereira, Mariane de Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/14414
Resumo: This study aimed at evaluating the effect of genotypes of Eucalyptus dunnii on in vitro establishment and also the influence of genotypes grown in different concentrations of 6-Benzylaminopurine (BAP) on in vitro multiplication. Explants were obtained from 10 parent plants selected in the field as a function of their superior phenotype characteristics. For in vitro establishment, 10 genotypes were evaluated, while in vitro multiplication consisted of 30 treatments which corresponded to combinations of six genotypes found to succeed in the in vitro establishment and five BAP concentrations (0, 0.25, 0.50, 0.75, and 1.0 mg L-1). Different behaviors were observed regarding the genotypes as to in vitro establishment rates, in which genotypes 3, 6 and 7 had establishment rates of over 70%, against 40% to 6.6% for the other genotypes. Genotypes 1, 5, 8 and 9 were later discarded due to the reduced number of explants successfully established. Factors such as microbial contamination and phenol oxidation posed a threat to in vitro establishment. The genotypes of Eucalyptus dunnii interacted differently with BAP in in vitro multiplication, noting that the concentration 0.50 mg L-1 positively influenced bud formation on the explants in most genotypes. Hyperhydricity was relatively low when 0.50 mg L-1 BAP was used and thus does not pose a threat to in vitro multiplication of nodal segments of Eucalyptus dunnii. 
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spelling In vitro establishment and multiplication of genotypes of Eucalyptus dunnii MaidenEstabelecimento e multiplicação in vitro de genótipos de Eucalyptus dunnii MaidenVegetative propagationTissue cultureMicropropagationPropagação vegetativaCultura de tecidosMicropropagaçãoThis study aimed at evaluating the effect of genotypes of Eucalyptus dunnii on in vitro establishment and also the influence of genotypes grown in different concentrations of 6-Benzylaminopurine (BAP) on in vitro multiplication. Explants were obtained from 10 parent plants selected in the field as a function of their superior phenotype characteristics. For in vitro establishment, 10 genotypes were evaluated, while in vitro multiplication consisted of 30 treatments which corresponded to combinations of six genotypes found to succeed in the in vitro establishment and five BAP concentrations (0, 0.25, 0.50, 0.75, and 1.0 mg L-1). Different behaviors were observed regarding the genotypes as to in vitro establishment rates, in which genotypes 3, 6 and 7 had establishment rates of over 70%, against 40% to 6.6% for the other genotypes. Genotypes 1, 5, 8 and 9 were later discarded due to the reduced number of explants successfully established. Factors such as microbial contamination and phenol oxidation posed a threat to in vitro establishment. The genotypes of Eucalyptus dunnii interacted differently with BAP in in vitro multiplication, noting that the concentration 0.50 mg L-1 positively influenced bud formation on the explants in most genotypes. Hyperhydricity was relatively low when 0.50 mg L-1 BAP was used and thus does not pose a threat to in vitro multiplication of nodal segments of Eucalyptus dunnii. Conduziu-se este trabalho, com o objetivo de avaliar o efeito de genótipos de Eucalyptus dunnii no estabelecimento in vitro e a influência de genótipos cultivados em diferentes concentrações de 6-Benzilaminopurina (BAP) na multiplicação in vitro. Os explantes foram oriundos de 10 matrizes, selecionadas a campo em função de características fenotípicas superiores. Para o estabelecimento in vitro foram avaliados 10 genótipos, e, para a multiplicação in vitro, 30 tratamentos, que corresponderam às combinações de seis genótipos que obtiveram sucesso no estabelecimento in vitro com cinco concentrações de BAP (0; 0,25; 0,50; 0,75; e 1,0 mg L-1). Constatou-se comportamento diferenciado dos genótipos em relação ao estabelecimento in vitro, sendo que os genótipos 3, 6 e 7 apresentaram estabelecimento superior a 70%, enquanto para os demais as médias variaram entre 40 e 6,6%. Os genótipos 1, 5, 8 e 9 foram, posteriormente, descartados em virtude do número reduzido de explantes estabelecidos. A contaminação microbiana e a oxidação fenólica constituíram fatores que comprometem o estabelecimento in vitro. Os genótipos de Eucalyptus dunnii interagem de maneira diferenciada com BAP na multiplicação in vitro, porém a concentração 0,50 mg L-1 influencia positivamente a formação de gemas por explante na maior parte dos genótipos estudados. A hiperhidricidade é relativamente baixa na presença de 0,50 mg L-1 de BAP, não comprometendo a multiplicação in vitro de segmentos nodais de Eucalyptus dunnii.Universidade Federal de Lavras (UFLA)2016-04-062017-08-01T20:14:36Z2017-08-01T20:14:36Z2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfNAVROSKI, M. C. et al. In vitro establishment and multiplication of genotypes of Eucalyptus dunnii Maiden. CERNE, Lavras, v. 20, n. 1, p. 139-146, jan./mar. 2014.http://repositorio.ufla.br/jspui/handle/1/144142317-63420104-7760reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAenghttp://www.cerne.ufla.br/site/index.php/CERNE/article/view/970/743Copyright (c) 2016 CERNEAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessNavroski, Marcio CarlosReiniger, Lia Rejane SilveiraAraújo, Maristela MachadoCurti, Aline RitterPereira, Mariane de Oliveira2021-05-03T01:18:39Zoai:localhost:1/14414Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-05-03T01:18:39Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv In vitro establishment and multiplication of genotypes of Eucalyptus dunnii Maiden
Estabelecimento e multiplicação in vitro de genótipos de Eucalyptus dunnii Maiden
title In vitro establishment and multiplication of genotypes of Eucalyptus dunnii Maiden
spellingShingle In vitro establishment and multiplication of genotypes of Eucalyptus dunnii Maiden
Navroski, Marcio Carlos
Vegetative propagation
Tissue culture
Micropropagation
Propagação vegetativa
Cultura de tecidos
Micropropagação
title_short In vitro establishment and multiplication of genotypes of Eucalyptus dunnii Maiden
title_full In vitro establishment and multiplication of genotypes of Eucalyptus dunnii Maiden
title_fullStr In vitro establishment and multiplication of genotypes of Eucalyptus dunnii Maiden
title_full_unstemmed In vitro establishment and multiplication of genotypes of Eucalyptus dunnii Maiden
title_sort In vitro establishment and multiplication of genotypes of Eucalyptus dunnii Maiden
author Navroski, Marcio Carlos
author_facet Navroski, Marcio Carlos
Reiniger, Lia Rejane Silveira
Araújo, Maristela Machado
Curti, Aline Ritter
Pereira, Mariane de Oliveira
author_role author
author2 Reiniger, Lia Rejane Silveira
Araújo, Maristela Machado
Curti, Aline Ritter
Pereira, Mariane de Oliveira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Navroski, Marcio Carlos
Reiniger, Lia Rejane Silveira
Araújo, Maristela Machado
Curti, Aline Ritter
Pereira, Mariane de Oliveira
dc.subject.por.fl_str_mv Vegetative propagation
Tissue culture
Micropropagation
Propagação vegetativa
Cultura de tecidos
Micropropagação
topic Vegetative propagation
Tissue culture
Micropropagation
Propagação vegetativa
Cultura de tecidos
Micropropagação
description This study aimed at evaluating the effect of genotypes of Eucalyptus dunnii on in vitro establishment and also the influence of genotypes grown in different concentrations of 6-Benzylaminopurine (BAP) on in vitro multiplication. Explants were obtained from 10 parent plants selected in the field as a function of their superior phenotype characteristics. For in vitro establishment, 10 genotypes were evaluated, while in vitro multiplication consisted of 30 treatments which corresponded to combinations of six genotypes found to succeed in the in vitro establishment and five BAP concentrations (0, 0.25, 0.50, 0.75, and 1.0 mg L-1). Different behaviors were observed regarding the genotypes as to in vitro establishment rates, in which genotypes 3, 6 and 7 had establishment rates of over 70%, against 40% to 6.6% for the other genotypes. Genotypes 1, 5, 8 and 9 were later discarded due to the reduced number of explants successfully established. Factors such as microbial contamination and phenol oxidation posed a threat to in vitro establishment. The genotypes of Eucalyptus dunnii interacted differently with BAP in in vitro multiplication, noting that the concentration 0.50 mg L-1 positively influenced bud formation on the explants in most genotypes. Hyperhydricity was relatively low when 0.50 mg L-1 BAP was used and thus does not pose a threat to in vitro multiplication of nodal segments of Eucalyptus dunnii. 
publishDate 2016
dc.date.none.fl_str_mv 2016-04-06
2017-08-01T20:14:36Z
2017-08-01T20:14:36Z
2017-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv NAVROSKI, M. C. et al. In vitro establishment and multiplication of genotypes of Eucalyptus dunnii Maiden. CERNE, Lavras, v. 20, n. 1, p. 139-146, jan./mar. 2014.
http://repositorio.ufla.br/jspui/handle/1/14414
identifier_str_mv NAVROSKI, M. C. et al. In vitro establishment and multiplication of genotypes of Eucalyptus dunnii Maiden. CERNE, Lavras, v. 20, n. 1, p. 139-146, jan./mar. 2014.
url http://repositorio.ufla.br/jspui/handle/1/14414
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.cerne.ufla.br/site/index.php/CERNE/article/view/970/743
dc.rights.driver.fl_str_mv Copyright (c) 2016 CERNE
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2016 CERNE
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Lavras (UFLA)
publisher.none.fl_str_mv Universidade Federal de Lavras (UFLA)
dc.source.none.fl_str_mv 2317-6342
0104-7760
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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