Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification

Detalhes bibliográficos
Autor(a) principal: Nogueira, Gabriela Ferreira
Data de Publicação: 2013
Outros Autores: Pasqual, Moacir, Scherwinski-Pereira, Jonny Everson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/38369
Resumo: The objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet-vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L-1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L-1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival.
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spelling Survival of sugarcane shoot tips after cryopreservation by droplet-vitrificationSobrevivência de ápices caulinares de cana-de-açúcar após criopreservação por "droplet-vitrification"SaccharumIn vitro conservationPVS2RegenerationPlant vitrification solution 2 (PVS2)Conservação in vitroRegeneraçãoThe objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet-vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L-1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L-1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival.O objetivo deste trabalho foi avaliar a fitotoxicidade da solução de vitrificação de plantas (PVS2) e a sobrevivência de ápices caulinares de cana-de-açúcar, variedade SP716949, após criopreservação por "droplet-vitrification". Ápices caulinares foram pré-cultivados por 24 horas em meio MS com sacarose a 0,3 mol L-1 e expostos por 0, 20 ou 30 min a PVS2. Em seguida, os ápices foram mergulhados em nitrogênio líquido. O descongelamento em solução concentrada de sacarose (1,2 mol L-1) foi rápido. A PVS2 não tem efeito tóxico aos ápices que, no entanto, são sensíveis ao nitrogênio líquido. O melhor resultado ocorreu, quando os ápices foram mantidos por 20 min na solução PVS2, antes do congelamento, com 20% de sobrevivência.Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Secretaria de Pesquisa e Desenvolvimento (SPD)2019-12-20T18:02:17Z2019-12-20T18:02:17Z2013-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfNOGUEIRA, G. F.; PASQUAL, M.; SCHERWINSKI-PEREIRA, J. E. Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification. Pesquisa Agropecuária Brasileira, Brasília, v. 48, n. 11, p. 1524-1527, Nov. 2013. DOI: 10.1590/S0100-204X2013001100014.http://repositorio.ufla.br/jspui/handle/1/38369Pesquisa Agropecuária Brasileira (PAB)reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessNogueira, Gabriela FerreiraPasqual, MoacirScherwinski-Pereira, Jonny Eversoneng2023-05-26T18:51:08Zoai:localhost:1/38369Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:51:08Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
Sobrevivência de ápices caulinares de cana-de-açúcar após criopreservação por "droplet-vitrification"
title Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
spellingShingle Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
Nogueira, Gabriela Ferreira
Saccharum
In vitro conservation
PVS2
Regeneration
Plant vitrification solution 2 (PVS2)
Conservação in vitro
Regeneração
title_short Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
title_full Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
title_fullStr Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
title_full_unstemmed Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
title_sort Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification
author Nogueira, Gabriela Ferreira
author_facet Nogueira, Gabriela Ferreira
Pasqual, Moacir
Scherwinski-Pereira, Jonny Everson
author_role author
author2 Pasqual, Moacir
Scherwinski-Pereira, Jonny Everson
author2_role author
author
dc.contributor.author.fl_str_mv Nogueira, Gabriela Ferreira
Pasqual, Moacir
Scherwinski-Pereira, Jonny Everson
dc.subject.por.fl_str_mv Saccharum
In vitro conservation
PVS2
Regeneration
Plant vitrification solution 2 (PVS2)
Conservação in vitro
Regeneração
topic Saccharum
In vitro conservation
PVS2
Regeneration
Plant vitrification solution 2 (PVS2)
Conservação in vitro
Regeneração
description The objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet-vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L-1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L-1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
2019-12-20T18:02:17Z
2019-12-20T18:02:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv NOGUEIRA, G. F.; PASQUAL, M.; SCHERWINSKI-PEREIRA, J. E. Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification. Pesquisa Agropecuária Brasileira, Brasília, v. 48, n. 11, p. 1524-1527, Nov. 2013. DOI: 10.1590/S0100-204X2013001100014.
http://repositorio.ufla.br/jspui/handle/1/38369
identifier_str_mv NOGUEIRA, G. F.; PASQUAL, M.; SCHERWINSKI-PEREIRA, J. E. Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification. Pesquisa Agropecuária Brasileira, Brasília, v. 48, n. 11, p. 1524-1527, Nov. 2013. DOI: 10.1590/S0100-204X2013001100014.
url http://repositorio.ufla.br/jspui/handle/1/38369
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Secretaria de Pesquisa e Desenvolvimento (SPD)
publisher.none.fl_str_mv Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Secretaria de Pesquisa e Desenvolvimento (SPD)
dc.source.none.fl_str_mv Pesquisa Agropecuária Brasileira (PAB)
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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