Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of nellore and angus cattle
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/45578 |
Resumo: | Studies have shown that intramuscular adipogenesis and fibrogenesis may concomitantly occur in skeletal muscle of beef cattle. Thus, we hypothesized that the discrepancy of intramuscular fat content in beef from Nellore and Angus was associated with differences in intramuscular adipogenesis and fibrogenesis during the finishing phase. To test our hypothesis, longissimus muscle samples of Nellore (n = 6; BW = 372.5 ± 37.3 kg) and Angus (n = 6; BW = 382.8 ± 23.9 kg) cattle were collected for analysis of gene and protein expression, and quantification of intramuscular fat and collagen. Least-squares means were estimated for the effect of Breed and differences were considered at P ≤ 0.05. A greater intramuscular fat content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05). No differences were observed for mRNA expression of lipogenic and lipolytic markers ACC, FAS, FABP4, SERBP–1, CPT–2, LPL, and ACOX (P > 0.05) in skeletal muscle of Nellore and Angus cattle. Similarly, no differences were observed in mRNA expression of adipogenic markers Zfp423, PPARγ, and C/EBPα (P>0.05) However, a greater PPARγ protein content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05). A greater abundance of adipo/fibrogenic cells, evaluated by the PDGFRα content, was observed in skeletal muscle of Angus than Nellore cattle (P≤0.05). No differences in fibrogenesis were observed in skeletal muscle of Angus and Nellore cattle, which is in accordance with the lack of differences in intramuscular collagen content in beef from both breeds (P>0.05). These findings demonstrate that difference in intramuscular fat content is associated with a slightly enhanced adipogenesis in skeletal muscle of Angus compared to Nellore cattle, while no difference in fibrogenesis. |
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Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of nellore and angus cattleIntramuscular adipogenesisFibrogenesisGene expressionBeef cattleAdipogênese intramuscularFibrogêneseColágenoExpressão gênicaBovinocultura de corteStudies have shown that intramuscular adipogenesis and fibrogenesis may concomitantly occur in skeletal muscle of beef cattle. Thus, we hypothesized that the discrepancy of intramuscular fat content in beef from Nellore and Angus was associated with differences in intramuscular adipogenesis and fibrogenesis during the finishing phase. To test our hypothesis, longissimus muscle samples of Nellore (n = 6; BW = 372.5 ± 37.3 kg) and Angus (n = 6; BW = 382.8 ± 23.9 kg) cattle were collected for analysis of gene and protein expression, and quantification of intramuscular fat and collagen. Least-squares means were estimated for the effect of Breed and differences were considered at P ≤ 0.05. A greater intramuscular fat content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05). No differences were observed for mRNA expression of lipogenic and lipolytic markers ACC, FAS, FABP4, SERBP–1, CPT–2, LPL, and ACOX (P > 0.05) in skeletal muscle of Nellore and Angus cattle. Similarly, no differences were observed in mRNA expression of adipogenic markers Zfp423, PPARγ, and C/EBPα (P>0.05) However, a greater PPARγ protein content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05). A greater abundance of adipo/fibrogenic cells, evaluated by the PDGFRα content, was observed in skeletal muscle of Angus than Nellore cattle (P≤0.05). No differences in fibrogenesis were observed in skeletal muscle of Angus and Nellore cattle, which is in accordance with the lack of differences in intramuscular collagen content in beef from both breeds (P>0.05). These findings demonstrate that difference in intramuscular fat content is associated with a slightly enhanced adipogenesis in skeletal muscle of Angus compared to Nellore cattle, while no difference in fibrogenesis.PLOS2020-11-20T19:03:17Z2020-11-20T19:03:17Z2015-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMARTINS, T. S. et al. Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of nellore and angus cattle. PLoS ONE, San Francisco, v. 10, n. 10, e0139943, Oct. 2015. DOI:10.1371/journal.pone.0139943.http://repositorio.ufla.br/jspui/handle/1/45578Plos Onereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMartins, Taiane S.Sanglard, Letícia M. P.Silva, WalmirChizzotti, Mário L.Rennó, Luciana N.Serão, Nick V. L.Silva, Fabyano F. S.Guimarães, Simone E. F.Ladeira, Márcio M.Dodson, Michael V.Du, MinDuarte, Marcio S.eng2020-11-20T19:03:44Zoai:localhost:1/45578Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-11-20T19:03:44Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of nellore and angus cattle |
title |
Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of nellore and angus cattle |
spellingShingle |
Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of nellore and angus cattle Martins, Taiane S. Intramuscular adipogenesis Fibrogenesis Gene expression Beef cattle Adipogênese intramuscular Fibrogênese Colágeno Expressão gênica Bovinocultura de corte |
title_short |
Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of nellore and angus cattle |
title_full |
Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of nellore and angus cattle |
title_fullStr |
Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of nellore and angus cattle |
title_full_unstemmed |
Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of nellore and angus cattle |
title_sort |
Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of nellore and angus cattle |
author |
Martins, Taiane S. |
author_facet |
Martins, Taiane S. Sanglard, Letícia M. P. Silva, Walmir Chizzotti, Mário L. Rennó, Luciana N. Serão, Nick V. L. Silva, Fabyano F. S. Guimarães, Simone E. F. Ladeira, Márcio M. Dodson, Michael V. Du, Min Duarte, Marcio S. |
author_role |
author |
author2 |
Sanglard, Letícia M. P. Silva, Walmir Chizzotti, Mário L. Rennó, Luciana N. Serão, Nick V. L. Silva, Fabyano F. S. Guimarães, Simone E. F. Ladeira, Márcio M. Dodson, Michael V. Du, Min Duarte, Marcio S. |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Martins, Taiane S. Sanglard, Letícia M. P. Silva, Walmir Chizzotti, Mário L. Rennó, Luciana N. Serão, Nick V. L. Silva, Fabyano F. S. Guimarães, Simone E. F. Ladeira, Márcio M. Dodson, Michael V. Du, Min Duarte, Marcio S. |
dc.subject.por.fl_str_mv |
Intramuscular adipogenesis Fibrogenesis Gene expression Beef cattle Adipogênese intramuscular Fibrogênese Colágeno Expressão gênica Bovinocultura de corte |
topic |
Intramuscular adipogenesis Fibrogenesis Gene expression Beef cattle Adipogênese intramuscular Fibrogênese Colágeno Expressão gênica Bovinocultura de corte |
description |
Studies have shown that intramuscular adipogenesis and fibrogenesis may concomitantly occur in skeletal muscle of beef cattle. Thus, we hypothesized that the discrepancy of intramuscular fat content in beef from Nellore and Angus was associated with differences in intramuscular adipogenesis and fibrogenesis during the finishing phase. To test our hypothesis, longissimus muscle samples of Nellore (n = 6; BW = 372.5 ± 37.3 kg) and Angus (n = 6; BW = 382.8 ± 23.9 kg) cattle were collected for analysis of gene and protein expression, and quantification of intramuscular fat and collagen. Least-squares means were estimated for the effect of Breed and differences were considered at P ≤ 0.05. A greater intramuscular fat content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05). No differences were observed for mRNA expression of lipogenic and lipolytic markers ACC, FAS, FABP4, SERBP–1, CPT–2, LPL, and ACOX (P > 0.05) in skeletal muscle of Nellore and Angus cattle. Similarly, no differences were observed in mRNA expression of adipogenic markers Zfp423, PPARγ, and C/EBPα (P>0.05) However, a greater PPARγ protein content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05). A greater abundance of adipo/fibrogenic cells, evaluated by the PDGFRα content, was observed in skeletal muscle of Angus than Nellore cattle (P≤0.05). No differences in fibrogenesis were observed in skeletal muscle of Angus and Nellore cattle, which is in accordance with the lack of differences in intramuscular collagen content in beef from both breeds (P>0.05). These findings demonstrate that difference in intramuscular fat content is associated with a slightly enhanced adipogenesis in skeletal muscle of Angus compared to Nellore cattle, while no difference in fibrogenesis. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10 2020-11-20T19:03:17Z 2020-11-20T19:03:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MARTINS, T. S. et al. Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of nellore and angus cattle. PLoS ONE, San Francisco, v. 10, n. 10, e0139943, Oct. 2015. DOI:10.1371/journal.pone.0139943. http://repositorio.ufla.br/jspui/handle/1/45578 |
identifier_str_mv |
MARTINS, T. S. et al. Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of nellore and angus cattle. PLoS ONE, San Francisco, v. 10, n. 10, e0139943, Oct. 2015. DOI:10.1371/journal.pone.0139943. |
url |
http://repositorio.ufla.br/jspui/handle/1/45578 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
PLOS |
publisher.none.fl_str_mv |
PLOS |
dc.source.none.fl_str_mv |
Plos One reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835087798009856 |