In vitro toxicological prospection of fungicides containing Difenoconazole or Tebuconazole as active ingredients

Detalhes bibliográficos
Autor(a) principal: Trento, Marcus Vinícius Cardoso
Data de Publicação: 2022
Outros Autores: Cesar, Pedro Henrique Souza, Machado, Gustavo Henrique Andrade, Braga, Mariana Aparecida, Abreu, Tatiane Silva de, Vieira, Larissa Fonseca Andrade, Marcussi, Silvana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/55967
Resumo: The active ingredients (AI) tebuconazole and difenoconazole (classified as toxic to living organisms and potentially mutagen, carcinogen, or teratogen) are part of the composition of widely used fungicides in crops. In 2016 they were found in irregular quantities by ANVISA in their latest report emitted by the Program of Analysis of Agrochemical Waste in Food. Two commercial fungicides containing the active ingredients tebuconazole and difenoconazole were evaluated in cytogenotoxic assays and on different biological molecules. Both active ingredients altered the clotting time of the plasma, and were procoagulants in the majority of the evaluated doses. The two AI acted on blood thrombi exerting thrombotic action and confirming the observed procoagulant potential. In the proteolysis assay, the AI did not alter the structure of fibrinogen under the conditions evaluated. Tebuconazole and Difenoconazole were also cytotoxic to human erythrocytes, as well as induced phospholipid breakdown, confirming their toxicity on membranes. However, under the conditions evaluated, the AI did not alter significantly the mitochondrial succinate dehydrogenase activity and did not induce DNA fragmentation. Assuming that changes in human cells and molecules have a cumulative effect, the toxic potential of fungicides might be greater when chronic exposure to their active principles occurs.
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spelling In vitro toxicological prospection of fungicides containing Difenoconazole or Tebuconazole as active ingredientsProspecção toxicológica in vitro de fungicidas contendo Difenoconazol ou Tebuconazol como ingredientes ativosThrombolytic activityCoagulant activityHemolytic activityProteolysis activityPhospholipase activityAtividade trombolíticaAtividade coagulanteAtividade hemolíticaAtividade de proteóliseAtividade de fosfolipaseThe active ingredients (AI) tebuconazole and difenoconazole (classified as toxic to living organisms and potentially mutagen, carcinogen, or teratogen) are part of the composition of widely used fungicides in crops. In 2016 they were found in irregular quantities by ANVISA in their latest report emitted by the Program of Analysis of Agrochemical Waste in Food. Two commercial fungicides containing the active ingredients tebuconazole and difenoconazole were evaluated in cytogenotoxic assays and on different biological molecules. Both active ingredients altered the clotting time of the plasma, and were procoagulants in the majority of the evaluated doses. The two AI acted on blood thrombi exerting thrombotic action and confirming the observed procoagulant potential. In the proteolysis assay, the AI did not alter the structure of fibrinogen under the conditions evaluated. Tebuconazole and Difenoconazole were also cytotoxic to human erythrocytes, as well as induced phospholipid breakdown, confirming their toxicity on membranes. However, under the conditions evaluated, the AI did not alter significantly the mitochondrial succinate dehydrogenase activity and did not induce DNA fragmentation. Assuming that changes in human cells and molecules have a cumulative effect, the toxic potential of fungicides might be greater when chronic exposure to their active principles occurs.Os ingredientes ativos (IA) tebuconazol e difenoconazol (classificados como tóxicos aos organismos vivos e potencialmente mutagênicos, cancerígenos ou teratógenos) fazem parte da composição de fungicidas amplamente utilizados nas lavouras. Em 2016 foram encontrados em quantidades irregulares pela ANVISA em seu último relatório emitido pelo Programa de Análise de Resíduos Agroquímicos em Alimentos. Dois fungicidas comerciais contendo os princípios ativos tebuconazol e difenoconazol foram avaliados em ensaios citogenotóxicos e em diferentes moléculas biológicas. Ambos os princípios ativos alteraram o tempo de coagulação do plasma e foram pró-coagulantes na maioria das doses avaliadas. Os dois IA atuaram sobre os trombos sanguíneos exercendo ação trombótica e confirmando o potencial pró-coagulante observado. No ensaio de proteólise, o IA não alterou a estrutura do fibrinogênio nas condições avaliadas. Tebuconazol e Difenoconazol também foram citotóxicos para eritrócitos humanos, assim como induziram a quebra de fosfolipídios, confirmando sua toxicidade em membranas. No entanto, nas condições avaliadas, o IA não alterou significativamente a atividade da succinato desidrogenase mitocondrial e não induziu a fragmentação do DNA. Assumindo que as alterações nas células e moléculas humanas têm um efeito cumulativo, o potencial tóxico dos fungicidas pode ser maior quando ocorre exposição crônica aos seus princípios ativos.Universidade Federal do Paraná (UFPR)2023-02-08T13:01:15Z2023-02-08T13:01:15Z2022-09-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfTRENTO, M.V.C. et al. In vitro toxicological prospection of fungicides containing Difenoconazole or Tebuconazole as active ingredients. Acta Biológica Paranaense, [S.l.], v. 51, p. 1-16, 2022. DOI: 10.5380/abp.v51i1.84716.http://repositorio.ufla.br/jspui/handle/1/55967Acta Biológica Paranaensereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessTrento, Marcus Vinícius CardosoCesar, Pedro Henrique SouzaMachado, Gustavo Henrique AndradeBraga, Mariana AparecidaAbreu, Tatiane Silva deVieira, Larissa Fonseca AndradeMarcussi, Silvanaeng2023-02-08T13:01:15Zoai:localhost:1/55967Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-02-08T13:01:15Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv In vitro toxicological prospection of fungicides containing Difenoconazole or Tebuconazole as active ingredients
Prospecção toxicológica in vitro de fungicidas contendo Difenoconazol ou Tebuconazol como ingredientes ativos
title In vitro toxicological prospection of fungicides containing Difenoconazole or Tebuconazole as active ingredients
spellingShingle In vitro toxicological prospection of fungicides containing Difenoconazole or Tebuconazole as active ingredients
Trento, Marcus Vinícius Cardoso
Thrombolytic activity
Coagulant activity
Hemolytic activity
Proteolysis activity
Phospholipase activity
Atividade trombolítica
Atividade coagulante
Atividade hemolítica
Atividade de proteólise
Atividade de fosfolipase
title_short In vitro toxicological prospection of fungicides containing Difenoconazole or Tebuconazole as active ingredients
title_full In vitro toxicological prospection of fungicides containing Difenoconazole or Tebuconazole as active ingredients
title_fullStr In vitro toxicological prospection of fungicides containing Difenoconazole or Tebuconazole as active ingredients
title_full_unstemmed In vitro toxicological prospection of fungicides containing Difenoconazole or Tebuconazole as active ingredients
title_sort In vitro toxicological prospection of fungicides containing Difenoconazole or Tebuconazole as active ingredients
author Trento, Marcus Vinícius Cardoso
author_facet Trento, Marcus Vinícius Cardoso
Cesar, Pedro Henrique Souza
Machado, Gustavo Henrique Andrade
Braga, Mariana Aparecida
Abreu, Tatiane Silva de
Vieira, Larissa Fonseca Andrade
Marcussi, Silvana
author_role author
author2 Cesar, Pedro Henrique Souza
Machado, Gustavo Henrique Andrade
Braga, Mariana Aparecida
Abreu, Tatiane Silva de
Vieira, Larissa Fonseca Andrade
Marcussi, Silvana
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Trento, Marcus Vinícius Cardoso
Cesar, Pedro Henrique Souza
Machado, Gustavo Henrique Andrade
Braga, Mariana Aparecida
Abreu, Tatiane Silva de
Vieira, Larissa Fonseca Andrade
Marcussi, Silvana
dc.subject.por.fl_str_mv Thrombolytic activity
Coagulant activity
Hemolytic activity
Proteolysis activity
Phospholipase activity
Atividade trombolítica
Atividade coagulante
Atividade hemolítica
Atividade de proteólise
Atividade de fosfolipase
topic Thrombolytic activity
Coagulant activity
Hemolytic activity
Proteolysis activity
Phospholipase activity
Atividade trombolítica
Atividade coagulante
Atividade hemolítica
Atividade de proteólise
Atividade de fosfolipase
description The active ingredients (AI) tebuconazole and difenoconazole (classified as toxic to living organisms and potentially mutagen, carcinogen, or teratogen) are part of the composition of widely used fungicides in crops. In 2016 they were found in irregular quantities by ANVISA in their latest report emitted by the Program of Analysis of Agrochemical Waste in Food. Two commercial fungicides containing the active ingredients tebuconazole and difenoconazole were evaluated in cytogenotoxic assays and on different biological molecules. Both active ingredients altered the clotting time of the plasma, and were procoagulants in the majority of the evaluated doses. The two AI acted on blood thrombi exerting thrombotic action and confirming the observed procoagulant potential. In the proteolysis assay, the AI did not alter the structure of fibrinogen under the conditions evaluated. Tebuconazole and Difenoconazole were also cytotoxic to human erythrocytes, as well as induced phospholipid breakdown, confirming their toxicity on membranes. However, under the conditions evaluated, the AI did not alter significantly the mitochondrial succinate dehydrogenase activity and did not induce DNA fragmentation. Assuming that changes in human cells and molecules have a cumulative effect, the toxic potential of fungicides might be greater when chronic exposure to their active principles occurs.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-26
2023-02-08T13:01:15Z
2023-02-08T13:01:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv TRENTO, M.V.C. et al. In vitro toxicological prospection of fungicides containing Difenoconazole or Tebuconazole as active ingredients. Acta Biológica Paranaense, [S.l.], v. 51, p. 1-16, 2022. DOI: 10.5380/abp.v51i1.84716.
http://repositorio.ufla.br/jspui/handle/1/55967
identifier_str_mv TRENTO, M.V.C. et al. In vitro toxicological prospection of fungicides containing Difenoconazole or Tebuconazole as active ingredients. Acta Biológica Paranaense, [S.l.], v. 51, p. 1-16, 2022. DOI: 10.5380/abp.v51i1.84716.
url http://repositorio.ufla.br/jspui/handle/1/55967
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do Paraná (UFPR)
publisher.none.fl_str_mv Universidade Federal do Paraná (UFPR)
dc.source.none.fl_str_mv Acta Biológica Paranaense
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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