'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000500029 http://repositorio.ufla.br/jspui/handle/1/6294 |
Resumo: | The aim of this work was to evaluate the effect of 'Ponkan' mandarin (C. reticulata) x 'Pêra' sweet orange (C. sinensis) immature fruits storage and sucrose concentrations on embryos in vitro culture. Fruits with 3 to 4 cm in diameter were harvested and placed inside black polyethylene bags with lateral openings and stored at 5±1ºC during 135 days. Every 15 days a sample was removed, its embryos were excised and individually inoculated in test tubes containing 15 mL of MS medium (Murashige & Skoog, 1962) with sucrose (0, 1.5, 3, 6, 12, 18 and 24 g L-1) and 0.3 mg L-1 GA3 and 1 g L-1 activated charcoal. Those treatments rested 48 hours in the dark and later in a growth room at 27 ± 1ºC with a 16-h photoperiod and 32 µmol m-2 s-1 light intensity. Immature fruits can be stored for posterior excision and embryos culture. Fruits with 120 days after the pollination can be stored for at most 135 days without damaging the embryos viability. It was observed a better development of the aerial part and root system of plantlets from 'Ponkan' mandarin x 'Pêra' sweet orange embryos in MS medium with 12-18 g L-1 sucrose. |
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'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storageArmazenamento de frutos imaturos de tangerineira 'Ponkan' (Citrus reticulata Blanco)Embryo cultureMandarin - StoragePlant breedingCultura de embriõesTangerineira - ArmazenamentoPlantas - Melhoramento genéticoThe aim of this work was to evaluate the effect of 'Ponkan' mandarin (C. reticulata) x 'Pêra' sweet orange (C. sinensis) immature fruits storage and sucrose concentrations on embryos in vitro culture. Fruits with 3 to 4 cm in diameter were harvested and placed inside black polyethylene bags with lateral openings and stored at 5±1ºC during 135 days. Every 15 days a sample was removed, its embryos were excised and individually inoculated in test tubes containing 15 mL of MS medium (Murashige & Skoog, 1962) with sucrose (0, 1.5, 3, 6, 12, 18 and 24 g L-1) and 0.3 mg L-1 GA3 and 1 g L-1 activated charcoal. Those treatments rested 48 hours in the dark and later in a growth room at 27 ± 1ºC with a 16-h photoperiod and 32 µmol m-2 s-1 light intensity. Immature fruits can be stored for posterior excision and embryos culture. Fruits with 120 days after the pollination can be stored for at most 135 days without damaging the embryos viability. It was observed a better development of the aerial part and root system of plantlets from 'Ponkan' mandarin x 'Pêra' sweet orange embryos in MS medium with 12-18 g L-1 sucrose.Objetivou-se com este trabalho avaliar o efeito do armazenamento de frutos e concentrações de sacarose no cultivo in vitro de embriões imaturos de tangerineira 'Ponkan' (Citrus reticulata Blanco) x laranjeira 'Pêra' [Citrus sinensis (L.) Osb.]. Frutos com 3 a 4 cm de diâmetro foram coletados e colocados em sacos pretos de polietileno perfurados e armazenados a 5±1ºC por um período de 135 dias. A cada 15 dias, uma amostra foi retirada, seus embriões foram excisados e inoculados individualmente em tubos de ensaio contendo 15 mL de meio MS acrescido de sacarose (0; 1,5; 3; 6; 12; 18 e 24 g L-1), 0,3 mg L-1 GA3 e 1 g L-1 de carvão ativado. Os tratamentos permaneceram 48 horas no escuro e em seguida foram transferidos para sala de crescimento a 27±1ºC com fotoperíodo de 16 horas e 32 µmol m-2 s-1 de intensidade luminosa. Frutos imaturos podem ser armazenados e posteriormente utilizados na excisão e cultura de embriões. Frutos com 120 dias após a polinização podem ser armazenados por mais de 135 dias sem afetar a viabilidade dos embriões. Observou-se melhor desenvolvimento da parte aérea e sistema radicular de plântulas oriundas de embriões de tangerineira 'Ponkan' x laranjeira 'Pêra' em meio MS com 12-18 g L-1 de sacarose.Editora da Universidade Federal de Lavras2006-10-012015-04-30T13:33:55Z2015-04-30T13:33:55Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000500029PASQUAL, M. et al. 'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage. Ciência e Agrotecnologia, Lavras, v. 30, n. 5, p. 1017-1020, set./out. 2006.http://repositorio.ufla.br/jspui/handle/1/6294Ciência e Agrotecnologia v.30 n.5 2006reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAPasqual, MoacirDutra, Leonardo FerreiraAraujo, Aparecida Gomes deFigueiredo, Milene Alves deenginfo:eu-repo/semantics/openAccess2016-08-02T14:08:06Zoai:localhost:1/6294Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-08-02T14:08:06Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage Armazenamento de frutos imaturos de tangerineira 'Ponkan' (Citrus reticulata Blanco) |
title |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage |
spellingShingle |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage Pasqual, Moacir Embryo culture Mandarin - Storage Plant breeding Cultura de embriões Tangerineira - Armazenamento Plantas - Melhoramento genético |
title_short |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage |
title_full |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage |
title_fullStr |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage |
title_full_unstemmed |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage |
title_sort |
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage |
author |
Pasqual, Moacir |
author_facet |
Pasqual, Moacir Dutra, Leonardo Ferreira Araujo, Aparecida Gomes de Figueiredo, Milene Alves de |
author_role |
author |
author2 |
Dutra, Leonardo Ferreira Araujo, Aparecida Gomes de Figueiredo, Milene Alves de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pasqual, Moacir Dutra, Leonardo Ferreira Araujo, Aparecida Gomes de Figueiredo, Milene Alves de |
dc.subject.por.fl_str_mv |
Embryo culture Mandarin - Storage Plant breeding Cultura de embriões Tangerineira - Armazenamento Plantas - Melhoramento genético |
topic |
Embryo culture Mandarin - Storage Plant breeding Cultura de embriões Tangerineira - Armazenamento Plantas - Melhoramento genético |
description |
The aim of this work was to evaluate the effect of 'Ponkan' mandarin (C. reticulata) x 'Pêra' sweet orange (C. sinensis) immature fruits storage and sucrose concentrations on embryos in vitro culture. Fruits with 3 to 4 cm in diameter were harvested and placed inside black polyethylene bags with lateral openings and stored at 5±1ºC during 135 days. Every 15 days a sample was removed, its embryos were excised and individually inoculated in test tubes containing 15 mL of MS medium (Murashige & Skoog, 1962) with sucrose (0, 1.5, 3, 6, 12, 18 and 24 g L-1) and 0.3 mg L-1 GA3 and 1 g L-1 activated charcoal. Those treatments rested 48 hours in the dark and later in a growth room at 27 ± 1ºC with a 16-h photoperiod and 32 µmol m-2 s-1 light intensity. Immature fruits can be stored for posterior excision and embryos culture. Fruits with 120 days after the pollination can be stored for at most 135 days without damaging the embryos viability. It was observed a better development of the aerial part and root system of plantlets from 'Ponkan' mandarin x 'Pêra' sweet orange embryos in MS medium with 12-18 g L-1 sucrose. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10-01 2015-04-30T13:33:55Z 2015-04-30T13:33:55Z 2015-04-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000500029 PASQUAL, M. et al. 'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage. Ciência e Agrotecnologia, Lavras, v. 30, n. 5, p. 1017-1020, set./out. 2006. http://repositorio.ufla.br/jspui/handle/1/6294 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000500029 http://repositorio.ufla.br/jspui/handle/1/6294 |
identifier_str_mv |
PASQUAL, M. et al. 'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage. Ciência e Agrotecnologia, Lavras, v. 30, n. 5, p. 1017-1020, set./out. 2006. |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.30 n.5 2006 reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835212295438336 |