Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae

Detalhes bibliográficos
Autor(a) principal: Mendes, Ana Luisa Gomes
Data de Publicação: 2020
Outros Autores: Joaquim, Heloísa Di Matteo, Zamae, Mara Inês Stefanini, Assis, Ramon Meira, Peixoto, Jéssica Renata de Moura, Araújo, Margarida Maria Gomes de, Guedes, Antônio Carlos Martins, Oliveira, Edward José, Magalhães, Vanessa Peruhype, Pascoal-Xavier, Marcelo Antônio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/42385
Resumo: BACKGROUND Leprosy is an infectious-contagious disease caused by Mycobacterium leprae that remain endemic in 105 countries. This neglected disease has a wide range of clinical and histopathological manifestations that are related to the host inflammatory and immune responses. More recently, the inflammasome has assumed a relevant role in the inflammatory response against microbiological agents. However, the involvement of inflammasome in leprosy remains poorly understood. OBJECTIVES The aim is to associate biomarkers of inflammasome with the different immunopathological forms of leprosy. METHODS We performed an observational, cross-sectional, and comparative study of the immunophenotypic expression of inflammasome-associated proteins in immunopathological forms of leprosy of 99 skin lesion samples by immunohistochemistry. The intensity and percentage of NLRP3, Caspase-1, Caspases-4/5, interleukin-1β and interleukin-18 immunoreactivities in the inflammatory infiltrate of skin biopsies were evaluated. FINDINGS Strong expression of NLRP3 and inflammatory Caspases-4/5 were observed in lepromatous leprosy (lepromatous pole). In addition, were observed low expression of caspase-1, interleukin-1β, and interleukin-18 in tuberculoid and lepromatous leprosy. The interpolar or borderline form showed immunophenotype predominantly similar to the lepromatous pole. MAIN CONCLUSIONS Our results demonstrate that the NLRP3 inflammasome is inactive in leprosy, suggesting immune evasion of M. leprae.
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spelling Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium lepraeLeprosyInflammasomeCaspaseBACKGROUND Leprosy is an infectious-contagious disease caused by Mycobacterium leprae that remain endemic in 105 countries. This neglected disease has a wide range of clinical and histopathological manifestations that are related to the host inflammatory and immune responses. More recently, the inflammasome has assumed a relevant role in the inflammatory response against microbiological agents. However, the involvement of inflammasome in leprosy remains poorly understood. OBJECTIVES The aim is to associate biomarkers of inflammasome with the different immunopathological forms of leprosy. METHODS We performed an observational, cross-sectional, and comparative study of the immunophenotypic expression of inflammasome-associated proteins in immunopathological forms of leprosy of 99 skin lesion samples by immunohistochemistry. The intensity and percentage of NLRP3, Caspase-1, Caspases-4/5, interleukin-1β and interleukin-18 immunoreactivities in the inflammatory infiltrate of skin biopsies were evaluated. FINDINGS Strong expression of NLRP3 and inflammatory Caspases-4/5 were observed in lepromatous leprosy (lepromatous pole). In addition, were observed low expression of caspase-1, interleukin-1β, and interleukin-18 in tuberculoid and lepromatous leprosy. The interpolar or borderline form showed immunophenotype predominantly similar to the lepromatous pole. MAIN CONCLUSIONS Our results demonstrate that the NLRP3 inflammasome is inactive in leprosy, suggesting immune evasion of M. leprae.Instituto Oswaldo Cruz, Ministério da Saúde2020-08-12T18:41:47Z2020-08-12T18:41:47Z2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMENDES, A. L. G. et al. Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 115, 2020.http://repositorio.ufla.br/jspui/handle/1/42385Memórias do Instituto Oswaldo Cruzreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMendes, Ana Luisa GomesJoaquim, Heloísa Di MatteoZamae, Mara Inês StefaniniAssis, Ramon MeiraPeixoto, Jéssica Renata de MouraAraújo, Margarida Maria Gomes deGuedes, Antônio Carlos MartinsOliveira, Edward JoséMagalhães, Vanessa PeruhypePascoal-Xavier, Marcelo Antônioeng2020-08-12T18:41:48Zoai:localhost:1/42385Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-08-12T18:41:48Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae
title Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae
spellingShingle Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae
Mendes, Ana Luisa Gomes
Leprosy
Inflammasome
Caspase
title_short Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae
title_full Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae
title_fullStr Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae
title_full_unstemmed Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae
title_sort Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae
author Mendes, Ana Luisa Gomes
author_facet Mendes, Ana Luisa Gomes
Joaquim, Heloísa Di Matteo
Zamae, Mara Inês Stefanini
Assis, Ramon Meira
Peixoto, Jéssica Renata de Moura
Araújo, Margarida Maria Gomes de
Guedes, Antônio Carlos Martins
Oliveira, Edward José
Magalhães, Vanessa Peruhype
Pascoal-Xavier, Marcelo Antônio
author_role author
author2 Joaquim, Heloísa Di Matteo
Zamae, Mara Inês Stefanini
Assis, Ramon Meira
Peixoto, Jéssica Renata de Moura
Araújo, Margarida Maria Gomes de
Guedes, Antônio Carlos Martins
Oliveira, Edward José
Magalhães, Vanessa Peruhype
Pascoal-Xavier, Marcelo Antônio
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mendes, Ana Luisa Gomes
Joaquim, Heloísa Di Matteo
Zamae, Mara Inês Stefanini
Assis, Ramon Meira
Peixoto, Jéssica Renata de Moura
Araújo, Margarida Maria Gomes de
Guedes, Antônio Carlos Martins
Oliveira, Edward José
Magalhães, Vanessa Peruhype
Pascoal-Xavier, Marcelo Antônio
dc.subject.por.fl_str_mv Leprosy
Inflammasome
Caspase
topic Leprosy
Inflammasome
Caspase
description BACKGROUND Leprosy is an infectious-contagious disease caused by Mycobacterium leprae that remain endemic in 105 countries. This neglected disease has a wide range of clinical and histopathological manifestations that are related to the host inflammatory and immune responses. More recently, the inflammasome has assumed a relevant role in the inflammatory response against microbiological agents. However, the involvement of inflammasome in leprosy remains poorly understood. OBJECTIVES The aim is to associate biomarkers of inflammasome with the different immunopathological forms of leprosy. METHODS We performed an observational, cross-sectional, and comparative study of the immunophenotypic expression of inflammasome-associated proteins in immunopathological forms of leprosy of 99 skin lesion samples by immunohistochemistry. The intensity and percentage of NLRP3, Caspase-1, Caspases-4/5, interleukin-1β and interleukin-18 immunoreactivities in the inflammatory infiltrate of skin biopsies were evaluated. FINDINGS Strong expression of NLRP3 and inflammatory Caspases-4/5 were observed in lepromatous leprosy (lepromatous pole). In addition, were observed low expression of caspase-1, interleukin-1β, and interleukin-18 in tuberculoid and lepromatous leprosy. The interpolar or borderline form showed immunophenotype predominantly similar to the lepromatous pole. MAIN CONCLUSIONS Our results demonstrate that the NLRP3 inflammasome is inactive in leprosy, suggesting immune evasion of M. leprae.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-12T18:41:47Z
2020-08-12T18:41:47Z
2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv MENDES, A. L. G. et al. Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 115, 2020.
http://repositorio.ufla.br/jspui/handle/1/42385
identifier_str_mv MENDES, A. L. G. et al. Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 115, 2020.
url http://repositorio.ufla.br/jspui/handle/1/42385
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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