Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/42385 |
Resumo: | BACKGROUND Leprosy is an infectious-contagious disease caused by Mycobacterium leprae that remain endemic in 105 countries. This neglected disease has a wide range of clinical and histopathological manifestations that are related to the host inflammatory and immune responses. More recently, the inflammasome has assumed a relevant role in the inflammatory response against microbiological agents. However, the involvement of inflammasome in leprosy remains poorly understood. OBJECTIVES The aim is to associate biomarkers of inflammasome with the different immunopathological forms of leprosy. METHODS We performed an observational, cross-sectional, and comparative study of the immunophenotypic expression of inflammasome-associated proteins in immunopathological forms of leprosy of 99 skin lesion samples by immunohistochemistry. The intensity and percentage of NLRP3, Caspase-1, Caspases-4/5, interleukin-1β and interleukin-18 immunoreactivities in the inflammatory infiltrate of skin biopsies were evaluated. FINDINGS Strong expression of NLRP3 and inflammatory Caspases-4/5 were observed in lepromatous leprosy (lepromatous pole). In addition, were observed low expression of caspase-1, interleukin-1β, and interleukin-18 in tuberculoid and lepromatous leprosy. The interpolar or borderline form showed immunophenotype predominantly similar to the lepromatous pole. MAIN CONCLUSIONS Our results demonstrate that the NLRP3 inflammasome is inactive in leprosy, suggesting immune evasion of M. leprae. |
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Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium lepraeLeprosyInflammasomeCaspaseBACKGROUND Leprosy is an infectious-contagious disease caused by Mycobacterium leprae that remain endemic in 105 countries. This neglected disease has a wide range of clinical and histopathological manifestations that are related to the host inflammatory and immune responses. More recently, the inflammasome has assumed a relevant role in the inflammatory response against microbiological agents. However, the involvement of inflammasome in leprosy remains poorly understood. OBJECTIVES The aim is to associate biomarkers of inflammasome with the different immunopathological forms of leprosy. METHODS We performed an observational, cross-sectional, and comparative study of the immunophenotypic expression of inflammasome-associated proteins in immunopathological forms of leprosy of 99 skin lesion samples by immunohistochemistry. The intensity and percentage of NLRP3, Caspase-1, Caspases-4/5, interleukin-1β and interleukin-18 immunoreactivities in the inflammatory infiltrate of skin biopsies were evaluated. FINDINGS Strong expression of NLRP3 and inflammatory Caspases-4/5 were observed in lepromatous leprosy (lepromatous pole). In addition, were observed low expression of caspase-1, interleukin-1β, and interleukin-18 in tuberculoid and lepromatous leprosy. The interpolar or borderline form showed immunophenotype predominantly similar to the lepromatous pole. MAIN CONCLUSIONS Our results demonstrate that the NLRP3 inflammasome is inactive in leprosy, suggesting immune evasion of M. leprae.Instituto Oswaldo Cruz, Ministério da Saúde2020-08-12T18:41:47Z2020-08-12T18:41:47Z2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMENDES, A. L. G. et al. Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 115, 2020.http://repositorio.ufla.br/jspui/handle/1/42385Memórias do Instituto Oswaldo Cruzreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMendes, Ana Luisa GomesJoaquim, Heloísa Di MatteoZamae, Mara Inês StefaniniAssis, Ramon MeiraPeixoto, Jéssica Renata de MouraAraújo, Margarida Maria Gomes deGuedes, Antônio Carlos MartinsOliveira, Edward JoséMagalhães, Vanessa PeruhypePascoal-Xavier, Marcelo Antônioeng2020-08-12T18:41:48Zoai:localhost:1/42385Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-08-12T18:41:48Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae |
title |
Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae |
spellingShingle |
Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae Mendes, Ana Luisa Gomes Leprosy Inflammasome Caspase |
title_short |
Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae |
title_full |
Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae |
title_fullStr |
Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae |
title_full_unstemmed |
Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae |
title_sort |
Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae |
author |
Mendes, Ana Luisa Gomes |
author_facet |
Mendes, Ana Luisa Gomes Joaquim, Heloísa Di Matteo Zamae, Mara Inês Stefanini Assis, Ramon Meira Peixoto, Jéssica Renata de Moura Araújo, Margarida Maria Gomes de Guedes, Antônio Carlos Martins Oliveira, Edward José Magalhães, Vanessa Peruhype Pascoal-Xavier, Marcelo Antônio |
author_role |
author |
author2 |
Joaquim, Heloísa Di Matteo Zamae, Mara Inês Stefanini Assis, Ramon Meira Peixoto, Jéssica Renata de Moura Araújo, Margarida Maria Gomes de Guedes, Antônio Carlos Martins Oliveira, Edward José Magalhães, Vanessa Peruhype Pascoal-Xavier, Marcelo Antônio |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Mendes, Ana Luisa Gomes Joaquim, Heloísa Di Matteo Zamae, Mara Inês Stefanini Assis, Ramon Meira Peixoto, Jéssica Renata de Moura Araújo, Margarida Maria Gomes de Guedes, Antônio Carlos Martins Oliveira, Edward José Magalhães, Vanessa Peruhype Pascoal-Xavier, Marcelo Antônio |
dc.subject.por.fl_str_mv |
Leprosy Inflammasome Caspase |
topic |
Leprosy Inflammasome Caspase |
description |
BACKGROUND Leprosy is an infectious-contagious disease caused by Mycobacterium leprae that remain endemic in 105 countries. This neglected disease has a wide range of clinical and histopathological manifestations that are related to the host inflammatory and immune responses. More recently, the inflammasome has assumed a relevant role in the inflammatory response against microbiological agents. However, the involvement of inflammasome in leprosy remains poorly understood. OBJECTIVES The aim is to associate biomarkers of inflammasome with the different immunopathological forms of leprosy. METHODS We performed an observational, cross-sectional, and comparative study of the immunophenotypic expression of inflammasome-associated proteins in immunopathological forms of leprosy of 99 skin lesion samples by immunohistochemistry. The intensity and percentage of NLRP3, Caspase-1, Caspases-4/5, interleukin-1β and interleukin-18 immunoreactivities in the inflammatory infiltrate of skin biopsies were evaluated. FINDINGS Strong expression of NLRP3 and inflammatory Caspases-4/5 were observed in lepromatous leprosy (lepromatous pole). In addition, were observed low expression of caspase-1, interleukin-1β, and interleukin-18 in tuberculoid and lepromatous leprosy. The interpolar or borderline form showed immunophenotype predominantly similar to the lepromatous pole. MAIN CONCLUSIONS Our results demonstrate that the NLRP3 inflammasome is inactive in leprosy, suggesting immune evasion of M. leprae. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-12T18:41:47Z 2020-08-12T18:41:47Z 2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MENDES, A. L. G. et al. Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 115, 2020. http://repositorio.ufla.br/jspui/handle/1/42385 |
identifier_str_mv |
MENDES, A. L. G. et al. Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 115, 2020. |
url |
http://repositorio.ufla.br/jspui/handle/1/42385 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835227835334656 |