Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/12094 |
Resumo: | This study evaluates the effects of extender osmolality and composition on the cooling of Prochilodus lineatus sperm. Sperm was diluted in six extenders: two compositions (powdered coconut water™ = ACP; Beltsville Thawing Solution™ = BTS) x three osmolalities (285, 325, and 365 mOsm/kg) plus an undiluted control, and stored at 6-8°C. Motility rate and velocities (curvilinear, straight line, and average path) were determined every other day. Osmolality did not affect the quality of cooled sperm, thus data were pooled. Motility was higher on d 0 compared to the other days and diluted samples (85-90%) yielded higher motility than control (75%). On d 2, motility was higher in BTS-diluted samples and control, but on d 4 and 6, control yielded the highest motility. Velocities decreased from d 0 to 6 in diluted samples, but not in control. On d 0, velocities were higher in BTS-diluted sperm, but, on d 2, 4, and 6, control yielded higher velocities despite of the large variation among males. Thus P. lineatus sperm should be stored in BTS or without dilution, for a maximum of two days at 6-8°C. Extender osmolality between 285 and 365 mOsm/kg does not affect sperm quality during cold storage. |
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Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) spermComputer-assisted sperm analyzerCoolingMotilitySemenVelocityThis study evaluates the effects of extender osmolality and composition on the cooling of Prochilodus lineatus sperm. Sperm was diluted in six extenders: two compositions (powdered coconut water™ = ACP; Beltsville Thawing Solution™ = BTS) x three osmolalities (285, 325, and 365 mOsm/kg) plus an undiluted control, and stored at 6-8°C. Motility rate and velocities (curvilinear, straight line, and average path) were determined every other day. Osmolality did not affect the quality of cooled sperm, thus data were pooled. Motility was higher on d 0 compared to the other days and diluted samples (85-90%) yielded higher motility than control (75%). On d 2, motility was higher in BTS-diluted samples and control, but on d 4 and 6, control yielded the highest motility. Velocities decreased from d 0 to 6 in diluted samples, but not in control. On d 0, velocities were higher in BTS-diluted sperm, but, on d 2, 4, and 6, control yielded higher velocities despite of the large variation among males. Thus P. lineatus sperm should be stored in BTS or without dilution, for a maximum of two days at 6-8°C. Extender osmolality between 285 and 365 mOsm/kg does not affect sperm quality during cold storage.Neste trabalho avaliou-se os efeitos da osmolalidade e da composição do diluidor no sêmen de Prochilodus lineatus, após o resfriamento. O sêmen foi diluído em seis diluidores: duas composições (água de coco em pó® = ACP; Beltsville Thawing Solution® = BTS) x três osmolalidades (285, 325 e 365 mOsm/kg) mais uma alíquota sem diluição como controle e armazenadas a 6-8°C. A taxa de motilidade e velocidades (curvilinear, retilinear e média de percurso) foram determinadas a cada dois dias. A osmolalidade não afetou a qualidade do sêmen resfriado, dessa forma foi feito um ‘pool’ desses dados. A motilidade foi maior no d 0 comparado aos outros dias e as amostras diluídas (85-90%) apresentaram as maiores motilidades do que o controle (75%). No d 2, a motilidade foi maior nas amostras diluídas em BTS e controle, mas nos d 4 e 6, o sêmen controle apresentou as maiores motilidades. As velocidades diminuíram do d 0 para o d 6 nas amostras diluídas, mas não no controle. No d 0, as velocidades foram maiores nas amostras diluídas em BTS, mas, nos d 2, 4 e 6, o controle apresentou as maiores velocidades apesar da grande variação entre os machos. Assim, o sêmen de P. lineatus deve ser resfriado em BTS ou sem diluição (controle), por no máximo dois dias a 6-8°C. A osmolalidade do diluidor entre 285 e 365 mOsm/kg não afeta a qualidade do sêmen durante o resfriamento.Sociedade Brasileira de Ictiologia2016-12-19T18:41:58Z2016-12-19T18:41:58Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfVIVEIROS, A. T. de M.; TAFFAREL, T. R.; LEAL, M. de C. Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm. Neotropical Ichthyology, Porto Alegre, v. 12, n. 3, p. 643-648, 2014.http://repositorio.ufla.br/jspui/handle/1/12094Neotropical Ichthyologyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAViveiros, Ana Tereza de MendonçaTaffarel, Tais ReginaLeal, Marcelo de Castroinfo:eu-repo/semantics/openAccesseng2016-12-19T18:41:58Zoai:localhost:1/12094Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-12-19T18:41:58Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm |
title |
Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm |
spellingShingle |
Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm Viveiros, Ana Tereza de Mendonça Computer-assisted sperm analyzer Cooling Motility Semen Velocity |
title_short |
Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm |
title_full |
Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm |
title_fullStr |
Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm |
title_full_unstemmed |
Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm |
title_sort |
Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm |
author |
Viveiros, Ana Tereza de Mendonça |
author_facet |
Viveiros, Ana Tereza de Mendonça Taffarel, Tais Regina Leal, Marcelo de Castro |
author_role |
author |
author2 |
Taffarel, Tais Regina Leal, Marcelo de Castro |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Viveiros, Ana Tereza de Mendonça Taffarel, Tais Regina Leal, Marcelo de Castro |
dc.subject.por.fl_str_mv |
Computer-assisted sperm analyzer Cooling Motility Semen Velocity |
topic |
Computer-assisted sperm analyzer Cooling Motility Semen Velocity |
description |
This study evaluates the effects of extender osmolality and composition on the cooling of Prochilodus lineatus sperm. Sperm was diluted in six extenders: two compositions (powdered coconut water™ = ACP; Beltsville Thawing Solution™ = BTS) x three osmolalities (285, 325, and 365 mOsm/kg) plus an undiluted control, and stored at 6-8°C. Motility rate and velocities (curvilinear, straight line, and average path) were determined every other day. Osmolality did not affect the quality of cooled sperm, thus data were pooled. Motility was higher on d 0 compared to the other days and diluted samples (85-90%) yielded higher motility than control (75%). On d 2, motility was higher in BTS-diluted samples and control, but on d 4 and 6, control yielded the highest motility. Velocities decreased from d 0 to 6 in diluted samples, but not in control. On d 0, velocities were higher in BTS-diluted sperm, but, on d 2, 4, and 6, control yielded higher velocities despite of the large variation among males. Thus P. lineatus sperm should be stored in BTS or without dilution, for a maximum of two days at 6-8°C. Extender osmolality between 285 and 365 mOsm/kg does not affect sperm quality during cold storage. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2016-12-19T18:41:58Z 2016-12-19T18:41:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
VIVEIROS, A. T. de M.; TAFFAREL, T. R.; LEAL, M. de C. Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm. Neotropical Ichthyology, Porto Alegre, v. 12, n. 3, p. 643-648, 2014. http://repositorio.ufla.br/jspui/handle/1/12094 |
identifier_str_mv |
VIVEIROS, A. T. de M.; TAFFAREL, T. R.; LEAL, M. de C. Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm. Neotropical Ichthyology, Porto Alegre, v. 12, n. 3, p. 643-648, 2014. |
url |
http://repositorio.ufla.br/jspui/handle/1/12094 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ictiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ictiologia |
dc.source.none.fl_str_mv |
Neotropical Ichthyology reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835126691790848 |