Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]

Detalhes bibliográficos
Autor(a) principal: Diniz, Josefa Diva Nogueira
Data de Publicação: 2003
Outros Autores: Almeida, Jacqueline Leite, Teixeira, Ana Luisa de Andrade, Gomes, Ednardo Souza, Hernandez, Fernando Felipe Ferreyra
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000400028
http://repositorio.ufla.br/jspui/handle/1/5758
Resumo: Micropropagation is a promising tool that can be utilized for propagation and preservation of many medicinal species that are being explored in an indiscriminated form and are under the risk of extinction. This work had as an objective to induce the growth in vitro of macela clusters in MS culture medium with different concentrations of GA3 (0, 0.1 and 0.5 mgL-1) and BAP (0, 1.0 and 2.0 mgL-1). Macela clusters or buddings with 1.5 cm of average diameter obtained from in vitro established plants were inoculated in glass containing 40ml of culture medium and keept in a growing room with light (2000 lux) supplied by cool white fluorescent lamps for 16 hours daily at 26 ± 1oC. The experiment design was 3 x 3 fatorial using a total of nine treatments with 15 explants each. On the 30th day, the highest plant was observed in the treatment with 0.5 mgL-1 GA3 in the absence of BAP. The greatest number of buddings or diameter growing was observed in the highest levels of growing regulators. However in the presence of growing regulators there was a great number of ill-shaped and vitrified leaves.
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spelling Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]Giberelic acid and 6-benzilaminopurine on in vitro growth of macela [Egletes viscosa (L.) Less.]Cultura de tecidosProliferaçãoReguladores de crescimentoTissue cultureProliferationGrowth regulatorsMicropropagation is a promising tool that can be utilized for propagation and preservation of many medicinal species that are being explored in an indiscriminated form and are under the risk of extinction. This work had as an objective to induce the growth in vitro of macela clusters in MS culture medium with different concentrations of GA3 (0, 0.1 and 0.5 mgL-1) and BAP (0, 1.0 and 2.0 mgL-1). Macela clusters or buddings with 1.5 cm of average diameter obtained from in vitro established plants were inoculated in glass containing 40ml of culture medium and keept in a growing room with light (2000 lux) supplied by cool white fluorescent lamps for 16 hours daily at 26 ± 1oC. The experiment design was 3 x 3 fatorial using a total of nine treatments with 15 explants each. On the 30th day, the highest plant was observed in the treatment with 0.5 mgL-1 GA3 in the absence of BAP. The greatest number of buddings or diameter growing was observed in the highest levels of growing regulators. However in the presence of growing regulators there was a great number of ill-shaped and vitrified leaves.A micropropagação é uma ferramenta promissora que pode ser utilizada para propagação e preservação de muitas espécies de plantas medicinais que vêm sendo exploradas de forma indiscriminada e que estão em vias de extinção. Conduziu-se este trabalho com o objetivo de induzir o crescimento in vitro de brotações de macela, em meio de cultura MS com diferentes concentrações de GA3 (0; 0,1 e 0,5 mgL-1) e BAP (0; 1 e 2 mgL-1). Foram utilizadas brotações de macela com diâmetro médio de 1,5 cm, retiradas de plantas preestabelecidas in vitro, inoculadas em frascos contendo 40 ml do meio de cultura e mantidas em sala de crescimento com temperatura média de 26 ± 1oC, fotoperíodo de 16 h e intensidade luminosa em torno de 2000 lux fornecida por lâmpada tipo fluorescente branca fria. Utilizou-se como delineamento experimental um fatorial 3 x 3, num total de nove tratamentos com 15 explantes por tratamento. Aos 30 dias, verificou-se o maior crescimento em altura das plantas, no tratamento com 0,5 mgL-1 de GA3 na ausência de BAP. O maior número de brotações ou crescimento em diâmetro foi observado com maiores concentrações dos reguladores. Porém, na presença dos reguladores ocorreu um maior número de plantas com folhas hiperhídricas e malformadas.Editora da Universidade Federal de Lavras2003-08-012015-04-30T13:32:48Z2015-04-30T13:32:48Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000400028DINIZ, J. D. N. et al. Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]. Ciência e Agrotecnologia, Lavras, v. 27, n. 4, p. 934-938, ago. 2003.http://repositorio.ufla.br/jspui/handle/1/5758Ciência e Agrotecnologia v.27 n.4 2003reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLADiniz, Josefa Diva NogueiraAlmeida, Jacqueline LeiteTeixeira, Ana Luisa de AndradeGomes, Ednardo SouzaHernandez, Fernando Felipe Ferreyraporinfo:eu-repo/semantics/openAccess2016-03-15T19:04:25Zoai:localhost:1/5758Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-03-15T19:04:25Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]
Giberelic acid and 6-benzilaminopurine on in vitro growth of macela [Egletes viscosa (L.) Less.]
title Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]
spellingShingle Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]
Diniz, Josefa Diva Nogueira
Cultura de tecidos
Proliferação
Reguladores de crescimento
Tissue culture
Proliferation
Growth regulators
title_short Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]
title_full Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]
title_fullStr Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]
title_full_unstemmed Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]
title_sort Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]
author Diniz, Josefa Diva Nogueira
author_facet Diniz, Josefa Diva Nogueira
Almeida, Jacqueline Leite
Teixeira, Ana Luisa de Andrade
Gomes, Ednardo Souza
Hernandez, Fernando Felipe Ferreyra
author_role author
author2 Almeida, Jacqueline Leite
Teixeira, Ana Luisa de Andrade
Gomes, Ednardo Souza
Hernandez, Fernando Felipe Ferreyra
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Diniz, Josefa Diva Nogueira
Almeida, Jacqueline Leite
Teixeira, Ana Luisa de Andrade
Gomes, Ednardo Souza
Hernandez, Fernando Felipe Ferreyra
dc.subject.por.fl_str_mv Cultura de tecidos
Proliferação
Reguladores de crescimento
Tissue culture
Proliferation
Growth regulators
topic Cultura de tecidos
Proliferação
Reguladores de crescimento
Tissue culture
Proliferation
Growth regulators
description Micropropagation is a promising tool that can be utilized for propagation and preservation of many medicinal species that are being explored in an indiscriminated form and are under the risk of extinction. This work had as an objective to induce the growth in vitro of macela clusters in MS culture medium with different concentrations of GA3 (0, 0.1 and 0.5 mgL-1) and BAP (0, 1.0 and 2.0 mgL-1). Macela clusters or buddings with 1.5 cm of average diameter obtained from in vitro established plants were inoculated in glass containing 40ml of culture medium and keept in a growing room with light (2000 lux) supplied by cool white fluorescent lamps for 16 hours daily at 26 ± 1oC. The experiment design was 3 x 3 fatorial using a total of nine treatments with 15 explants each. On the 30th day, the highest plant was observed in the treatment with 0.5 mgL-1 GA3 in the absence of BAP. The greatest number of buddings or diameter growing was observed in the highest levels of growing regulators. However in the presence of growing regulators there was a great number of ill-shaped and vitrified leaves.
publishDate 2003
dc.date.none.fl_str_mv 2003-08-01
2015-04-30T13:32:48Z
2015-04-30T13:32:48Z
2015-04-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000400028
DINIZ, J. D. N. et al. Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]. Ciência e Agrotecnologia, Lavras, v. 27, n. 4, p. 934-938, ago. 2003.
http://repositorio.ufla.br/jspui/handle/1/5758
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000400028
http://repositorio.ufla.br/jspui/handle/1/5758
identifier_str_mv DINIZ, J. D. N. et al. Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]. Ciência e Agrotecnologia, Lavras, v. 27, n. 4, p. 934-938, ago. 2003.
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language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Editora da Universidade Federal de Lavras
publisher.none.fl_str_mv Editora da Universidade Federal de Lavras
dc.source.none.fl_str_mv Ciência e Agrotecnologia v.27 n.4 2003
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
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institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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