Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000400028 http://repositorio.ufla.br/jspui/handle/1/5758 |
Resumo: | Micropropagation is a promising tool that can be utilized for propagation and preservation of many medicinal species that are being explored in an indiscriminated form and are under the risk of extinction. This work had as an objective to induce the growth in vitro of macela clusters in MS culture medium with different concentrations of GA3 (0, 0.1 and 0.5 mgL-1) and BAP (0, 1.0 and 2.0 mgL-1). Macela clusters or buddings with 1.5 cm of average diameter obtained from in vitro established plants were inoculated in glass containing 40ml of culture medium and keept in a growing room with light (2000 lux) supplied by cool white fluorescent lamps for 16 hours daily at 26 ± 1oC. The experiment design was 3 x 3 fatorial using a total of nine treatments with 15 explants each. On the 30th day, the highest plant was observed in the treatment with 0.5 mgL-1 GA3 in the absence of BAP. The greatest number of buddings or diameter growing was observed in the highest levels of growing regulators. However in the presence of growing regulators there was a great number of ill-shaped and vitrified leaves. |
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Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]Giberelic acid and 6-benzilaminopurine on in vitro growth of macela [Egletes viscosa (L.) Less.]Cultura de tecidosProliferaçãoReguladores de crescimentoTissue cultureProliferationGrowth regulatorsMicropropagation is a promising tool that can be utilized for propagation and preservation of many medicinal species that are being explored in an indiscriminated form and are under the risk of extinction. This work had as an objective to induce the growth in vitro of macela clusters in MS culture medium with different concentrations of GA3 (0, 0.1 and 0.5 mgL-1) and BAP (0, 1.0 and 2.0 mgL-1). Macela clusters or buddings with 1.5 cm of average diameter obtained from in vitro established plants were inoculated in glass containing 40ml of culture medium and keept in a growing room with light (2000 lux) supplied by cool white fluorescent lamps for 16 hours daily at 26 ± 1oC. The experiment design was 3 x 3 fatorial using a total of nine treatments with 15 explants each. On the 30th day, the highest plant was observed in the treatment with 0.5 mgL-1 GA3 in the absence of BAP. The greatest number of buddings or diameter growing was observed in the highest levels of growing regulators. However in the presence of growing regulators there was a great number of ill-shaped and vitrified leaves.A micropropagação é uma ferramenta promissora que pode ser utilizada para propagação e preservação de muitas espécies de plantas medicinais que vêm sendo exploradas de forma indiscriminada e que estão em vias de extinção. Conduziu-se este trabalho com o objetivo de induzir o crescimento in vitro de brotações de macela, em meio de cultura MS com diferentes concentrações de GA3 (0; 0,1 e 0,5 mgL-1) e BAP (0; 1 e 2 mgL-1). Foram utilizadas brotações de macela com diâmetro médio de 1,5 cm, retiradas de plantas preestabelecidas in vitro, inoculadas em frascos contendo 40 ml do meio de cultura e mantidas em sala de crescimento com temperatura média de 26 ± 1oC, fotoperíodo de 16 h e intensidade luminosa em torno de 2000 lux fornecida por lâmpada tipo fluorescente branca fria. Utilizou-se como delineamento experimental um fatorial 3 x 3, num total de nove tratamentos com 15 explantes por tratamento. Aos 30 dias, verificou-se o maior crescimento em altura das plantas, no tratamento com 0,5 mgL-1 de GA3 na ausência de BAP. O maior número de brotações ou crescimento em diâmetro foi observado com maiores concentrações dos reguladores. Porém, na presença dos reguladores ocorreu um maior número de plantas com folhas hiperhídricas e malformadas.Editora da Universidade Federal de Lavras2003-08-012015-04-30T13:32:48Z2015-04-30T13:32:48Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000400028DINIZ, J. D. N. et al. Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]. Ciência e Agrotecnologia, Lavras, v. 27, n. 4, p. 934-938, ago. 2003.http://repositorio.ufla.br/jspui/handle/1/5758Ciência e Agrotecnologia v.27 n.4 2003reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLADiniz, Josefa Diva NogueiraAlmeida, Jacqueline LeiteTeixeira, Ana Luisa de AndradeGomes, Ednardo SouzaHernandez, Fernando Felipe Ferreyraporinfo:eu-repo/semantics/openAccess2016-03-15T19:04:25Zoai:localhost:1/5758Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-03-15T19:04:25Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.] Giberelic acid and 6-benzilaminopurine on in vitro growth of macela [Egletes viscosa (L.) Less.] |
title |
Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.] |
spellingShingle |
Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.] Diniz, Josefa Diva Nogueira Cultura de tecidos Proliferação Reguladores de crescimento Tissue culture Proliferation Growth regulators |
title_short |
Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.] |
title_full |
Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.] |
title_fullStr |
Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.] |
title_full_unstemmed |
Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.] |
title_sort |
Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.] |
author |
Diniz, Josefa Diva Nogueira |
author_facet |
Diniz, Josefa Diva Nogueira Almeida, Jacqueline Leite Teixeira, Ana Luisa de Andrade Gomes, Ednardo Souza Hernandez, Fernando Felipe Ferreyra |
author_role |
author |
author2 |
Almeida, Jacqueline Leite Teixeira, Ana Luisa de Andrade Gomes, Ednardo Souza Hernandez, Fernando Felipe Ferreyra |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Diniz, Josefa Diva Nogueira Almeida, Jacqueline Leite Teixeira, Ana Luisa de Andrade Gomes, Ednardo Souza Hernandez, Fernando Felipe Ferreyra |
dc.subject.por.fl_str_mv |
Cultura de tecidos Proliferação Reguladores de crescimento Tissue culture Proliferation Growth regulators |
topic |
Cultura de tecidos Proliferação Reguladores de crescimento Tissue culture Proliferation Growth regulators |
description |
Micropropagation is a promising tool that can be utilized for propagation and preservation of many medicinal species that are being explored in an indiscriminated form and are under the risk of extinction. This work had as an objective to induce the growth in vitro of macela clusters in MS culture medium with different concentrations of GA3 (0, 0.1 and 0.5 mgL-1) and BAP (0, 1.0 and 2.0 mgL-1). Macela clusters or buddings with 1.5 cm of average diameter obtained from in vitro established plants were inoculated in glass containing 40ml of culture medium and keept in a growing room with light (2000 lux) supplied by cool white fluorescent lamps for 16 hours daily at 26 ± 1oC. The experiment design was 3 x 3 fatorial using a total of nine treatments with 15 explants each. On the 30th day, the highest plant was observed in the treatment with 0.5 mgL-1 GA3 in the absence of BAP. The greatest number of buddings or diameter growing was observed in the highest levels of growing regulators. However in the presence of growing regulators there was a great number of ill-shaped and vitrified leaves. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-08-01 2015-04-30T13:32:48Z 2015-04-30T13:32:48Z 2015-04-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000400028 DINIZ, J. D. N. et al. Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]. Ciência e Agrotecnologia, Lavras, v. 27, n. 4, p. 934-938, ago. 2003. http://repositorio.ufla.br/jspui/handle/1/5758 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000400028 http://repositorio.ufla.br/jspui/handle/1/5758 |
identifier_str_mv |
DINIZ, J. D. N. et al. Ácido giberélico (ga3) e 6-benzilaminopurina (bap) no crescimento in vitro de macela [Egletes viscosa (L.) Less.]. Ciência e Agrotecnologia, Lavras, v. 27, n. 4, p. 934-938, ago. 2003. |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.27 n.4 2003 reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
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UFLA |
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UFLA |
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Repositório Institucional da UFLA |
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Repositório Institucional da UFLA |
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Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
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nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835087199272960 |