Alterações bioquímicas e hepáticas em ratos submetidos à uma dieta hiperlipídica/hiperenergética

• Autor(a) principal: Moura, Leandro Pereira de
• Data de Publicação: 2012
• Outros Autores: Dalia, Rodrigo Augusto, Araújo, Michel Barbosa de, Sponton, Amanda Christine da Silva, Pauli, José Rodrigo, Moura, Rodrigo Ferreira de, Mello, Maria Alice Rostom de
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UFLA
• Texto Completo: http://repositorio.ufla.br/jspui/handle/1/45941
• topic: Dieta hiperlipídica; Doença hepática gordurosa não alcoólica; Resistência à insulina; Sobrepeso; Diet; High-fat; Non-alcoholic fatty liver disease; Insulin resistance; Overweight

OBJECTIVE: The present study analyzed the biochemical and hepatic changes in adult rats fed a high-fat diet for two months. METHODS: Twenty Wistar rats 90 days old were divided into two groups, a control group consisting of normal weight rats fed a commercial rat chow and a diet group consisting of normal weight rats submitted to a semi-purified high-fat, high-energy diet. The animals in control group were kept on a commercial Purina® chow and those in diet group on a high-fat/high-energy diet containing 35% fat, of which 31% were from animal source (39% saturated fat) and 4% were from vegetable source (soybean oil). After 60 days of this experimental diet, the following were assessed: body weight, insulin sensitivity, blood glucose, serum insulin and free fatty acids, triglycerides, total lipids and hepatic lipogenic activity. RESULTS: Diet group presented higher body mass and insulin resistance. Blood glucose did not differ between the groups. A higher level of serum insulin and free fatty acids were found in diet group. Total lipids, triglycerides and lipogenic rate were also higher in group D. CONCLUSION: Therefore, the present findings demonstrate that two months of a high-fat/high-energy diet increases the body weight and hepatic free fatty acids and decreases insulin sensitivity of adult rats, typical signs of non-alcoholic fatty liver disease.