Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomics

Detalhes bibliográficos
Autor(a) principal: Weiss, Bruno
Data de Publicação: 2021
Outros Autores: Souza, Anna Carolina Oliveira, Constancio, Milena Tavares Lima, Alvarenga, Danillo Oliveira, Pylro, Victor S., Alves, Lucia M. Carareto, Varani, Alessandro M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/48717
Resumo: Second-generation biofuel production is in high demand, but lignocellulosic biomass’ complexity impairs its use due to the vast diversity of enzymes necessary to execute the complete saccharification. In nature, lignocellulose can be rapidly deconstructed due to the division of biochemical labor effectuated in bacterial communities. Here, we analyzed the lignocellulolytic potential of a bacterial consortium obtained from soil and dry straw leftover from a sugarcane milling plant. This consortium was cultivated for 20 weeks in aerobic conditions using sugarcane bagasse as a sole carbon source. Scanning electron microscopy and chemical analyses registered modification of the sugarcane fiber’s appearance and biochemical composition, indicating that this consortium can deconstruct cellulose and hemicellulose but no lignin. A total of 52 metagenome-assembled genomes from eight bacterial classes (Actinobacteria, Alphaproteobacteria, Bacilli, Bacteroidia, Cytophagia, Gammaproteobacteria, Oligoflexia, and Thermoleophilia) were recovered from the consortium, in which ~46% of species showed no relevant modification in their abundance during the 20 weeks of cultivation, suggesting a mostly stable consortium. Their CAZymes repertoire indicated that many of the most abundant species are known to deconstruct lignin (e.g., Chryseobacterium) and carry sequences related to hemicellulose and cellulose deconstruction (e.g., Chitinophaga, Niastella, Niabella, and Siphonobacter). Taken together, our results unraveled the bacterial diversity, enzymatic potential, and effectiveness of this lignocellulose-decomposing bacterial consortium
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spelling Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomicsLignocelluloseBiotechnologyMetabolic modelingBiofuelsCommunity dynamicsLignoceluloseBiotecnologiaModelagem metabólicaBiocombustíveisDinâmica da comunidadeSecond-generation biofuel production is in high demand, but lignocellulosic biomass’ complexity impairs its use due to the vast diversity of enzymes necessary to execute the complete saccharification. In nature, lignocellulose can be rapidly deconstructed due to the division of biochemical labor effectuated in bacterial communities. Here, we analyzed the lignocellulolytic potential of a bacterial consortium obtained from soil and dry straw leftover from a sugarcane milling plant. This consortium was cultivated for 20 weeks in aerobic conditions using sugarcane bagasse as a sole carbon source. Scanning electron microscopy and chemical analyses registered modification of the sugarcane fiber’s appearance and biochemical composition, indicating that this consortium can deconstruct cellulose and hemicellulose but no lignin. A total of 52 metagenome-assembled genomes from eight bacterial classes (Actinobacteria, Alphaproteobacteria, Bacilli, Bacteroidia, Cytophagia, Gammaproteobacteria, Oligoflexia, and Thermoleophilia) were recovered from the consortium, in which ~46% of species showed no relevant modification in their abundance during the 20 weeks of cultivation, suggesting a mostly stable consortium. Their CAZymes repertoire indicated that many of the most abundant species are known to deconstruct lignin (e.g., Chryseobacterium) and carry sequences related to hemicellulose and cellulose deconstruction (e.g., Chitinophaga, Niastella, Niabella, and Siphonobacter). Taken together, our results unraveled the bacterial diversity, enzymatic potential, and effectiveness of this lignocellulose-decomposing bacterial consortiumMDPI2021-12-21T18:09:47Z2021-12-21T18:09:47Z2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfWEISS, B. et al. Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomics. Microorganisms, [S. l.], v. 9, n. 5, 2021. DOI: /10.3390/microorganisms9050995.http://repositorio.ufla.br/jspui/handle/1/48717Microorganismsreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessWeiss, BrunoSouza, Anna Carolina OliveiraConstancio, Milena Tavares LimaAlvarenga, Danillo OliveiraPylro, Victor S.Alves, Lucia M. CararetoVarani, Alessandro M.eng2021-12-21T18:09:47Zoai:localhost:1/48717Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-12-21T18:09:47Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomics
title Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomics
spellingShingle Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomics
Weiss, Bruno
Lignocellulose
Biotechnology
Metabolic modeling
Biofuels
Community dynamics
Lignocelulose
Biotecnologia
Modelagem metabólica
Biocombustíveis
Dinâmica da comunidade
title_short Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomics
title_full Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomics
title_fullStr Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomics
title_full_unstemmed Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomics
title_sort Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomics
author Weiss, Bruno
author_facet Weiss, Bruno
Souza, Anna Carolina Oliveira
Constancio, Milena Tavares Lima
Alvarenga, Danillo Oliveira
Pylro, Victor S.
Alves, Lucia M. Carareto
Varani, Alessandro M.
author_role author
author2 Souza, Anna Carolina Oliveira
Constancio, Milena Tavares Lima
Alvarenga, Danillo Oliveira
Pylro, Victor S.
Alves, Lucia M. Carareto
Varani, Alessandro M.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Weiss, Bruno
Souza, Anna Carolina Oliveira
Constancio, Milena Tavares Lima
Alvarenga, Danillo Oliveira
Pylro, Victor S.
Alves, Lucia M. Carareto
Varani, Alessandro M.
dc.subject.por.fl_str_mv Lignocellulose
Biotechnology
Metabolic modeling
Biofuels
Community dynamics
Lignocelulose
Biotecnologia
Modelagem metabólica
Biocombustíveis
Dinâmica da comunidade
topic Lignocellulose
Biotechnology
Metabolic modeling
Biofuels
Community dynamics
Lignocelulose
Biotecnologia
Modelagem metabólica
Biocombustíveis
Dinâmica da comunidade
description Second-generation biofuel production is in high demand, but lignocellulosic biomass’ complexity impairs its use due to the vast diversity of enzymes necessary to execute the complete saccharification. In nature, lignocellulose can be rapidly deconstructed due to the division of biochemical labor effectuated in bacterial communities. Here, we analyzed the lignocellulolytic potential of a bacterial consortium obtained from soil and dry straw leftover from a sugarcane milling plant. This consortium was cultivated for 20 weeks in aerobic conditions using sugarcane bagasse as a sole carbon source. Scanning electron microscopy and chemical analyses registered modification of the sugarcane fiber’s appearance and biochemical composition, indicating that this consortium can deconstruct cellulose and hemicellulose but no lignin. A total of 52 metagenome-assembled genomes from eight bacterial classes (Actinobacteria, Alphaproteobacteria, Bacilli, Bacteroidia, Cytophagia, Gammaproteobacteria, Oligoflexia, and Thermoleophilia) were recovered from the consortium, in which ~46% of species showed no relevant modification in their abundance during the 20 weeks of cultivation, suggesting a mostly stable consortium. Their CAZymes repertoire indicated that many of the most abundant species are known to deconstruct lignin (e.g., Chryseobacterium) and carry sequences related to hemicellulose and cellulose deconstruction (e.g., Chitinophaga, Niastella, Niabella, and Siphonobacter). Taken together, our results unraveled the bacterial diversity, enzymatic potential, and effectiveness of this lignocellulose-decomposing bacterial consortium
publishDate 2021
dc.date.none.fl_str_mv 2021-12-21T18:09:47Z
2021-12-21T18:09:47Z
2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv WEISS, B. et al. Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomics. Microorganisms, [S. l.], v. 9, n. 5, 2021. DOI: /10.3390/microorganisms9050995.
http://repositorio.ufla.br/jspui/handle/1/48717
identifier_str_mv WEISS, B. et al. Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomics. Microorganisms, [S. l.], v. 9, n. 5, 2021. DOI: /10.3390/microorganisms9050995.
url http://repositorio.ufla.br/jspui/handle/1/48717
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Microorganisms
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1807835207201456128

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