Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa

Detalhes bibliográficos
Autor(a) principal: Chiavegatto, Raquel Bezerra
Data de Publicação: 2017
Outros Autores: Chaves, Ana Luisa Arantes, Silva, Izabela Caputo Assis, Lima, Luciana Alves Rodrigues dos Santos, Techio, Vânia Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/32721
Resumo: Solanum lycocarpum St.-Hil popularly known as ‘fruta-de-lobo’ or ‘lobeira’ is native to the Brazilian Cerrado, and used in folk medicine due to its phytotherapic properties. The action of S. lycocarpumon the cell cycle and chromosomes in order to demonstrate whether there are aneugenic and/or clastogenic effects is unknown. Thus, this study aimed at investigating the cytotoxic and genotoxic potential of methanol and hexane extracts of S. lycocarpumon growth and cell cycle of Lactuca sativaand Allium cepa. Roots from both species were exposed for 72 hours to methanol and hexane extracts with 50, 100, and 200 μg mL-1 of S. lycocarpum. Slides were prepared by the squash technique and then analyzed to determine the mitotic index and the total of chromosomal and nuclear abnormalities. The frequencies of chromosomal and nuclear abnormalities were high and significant with a dose-dependent effect, indicating that S. lycocarpumhas a cytotoxic and genotoxic action depending on the dose used on meristem cells of A. cepaand L. sativa.
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spelling Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepaEfeito citotóxicos e genotóxicos de extratos Solanum lycocarpum St.-Hil (Solanaceae) no ciclo celular de Lactuca sativa e Allium cepaAneugenicClastogenicMedicinal plantAneugênicoCerradoClastogênicoLobeiraPlanta medicinalSolanum lycocarpum St.-Hil popularly known as ‘fruta-de-lobo’ or ‘lobeira’ is native to the Brazilian Cerrado, and used in folk medicine due to its phytotherapic properties. The action of S. lycocarpumon the cell cycle and chromosomes in order to demonstrate whether there are aneugenic and/or clastogenic effects is unknown. Thus, this study aimed at investigating the cytotoxic and genotoxic potential of methanol and hexane extracts of S. lycocarpumon growth and cell cycle of Lactuca sativaand Allium cepa. Roots from both species were exposed for 72 hours to methanol and hexane extracts with 50, 100, and 200 μg mL-1 of S. lycocarpum. Slides were prepared by the squash technique and then analyzed to determine the mitotic index and the total of chromosomal and nuclear abnormalities. The frequencies of chromosomal and nuclear abnormalities were high and significant with a dose-dependent effect, indicating that S. lycocarpumhas a cytotoxic and genotoxic action depending on the dose used on meristem cells of A. cepaand L. sativa.Solanum lycocarpum St.-Hil (Solanaceae) conhecida popularmente como fruta-de-lobo ou lobeira é uma planta nativa do Cerrado brasileiro, utilizada na medicina popular nos tratamentos de diabetes, obesidade e redução do colesterol. Ainda não é conhecida a ação de S. lycocarpumsobre o ciclo celular e os cromossomos, demonstrando se possuem efeitos aneugênicos e/ou clastogênicos. O objetivo desse estudo foi investigar o potencial citotóxico e genotóxico dos extratos metanólico e hexânico de S. lycocarpumsob o crescimento e ciclo celular de Lactuca sativae Allium cepa. As raízes de ambas as espécies foram expostas por 72h aos extratos metanólico e hexânico com 50, 100 e 200 μg mL-1 de S. lycocarpum. As lâminas foram montadas pela técnica de esmagamento e em seguida foram analisadas, afim de determinar o índice mitótico e anormalidades cromossômicas e nucleares. As frequências de anormalidades cromossômicas e nucleares foram altas e significativas, com efeito dose-dependente e confirmando que S. lycocarpumtem ação citotóxica e genotóxica de acordo com a dose utilizada sobre as células meristemáticas de A. cepae L. sativa.Editora da Universidade Estadual de Maringá (Eduem)2019-02-04T11:49:48Z2019-02-04T11:49:48Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCHIAVEGATTO, R. B. et al. Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa. Acta Scientiarum. Biological Sciences, Maringá, v. 39, n. 2, p. 201-210, 2017.http://repositorio.ufla.br/jspui/handle/1/32721Acta Scientiarum. Biological Sciencesreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessChiavegatto, Raquel BezerraChaves, Ana Luisa ArantesSilva, Izabela Caputo AssisLima, Luciana Alves Rodrigues dos SantosTechio, Vânia Helenaeng2019-02-04T11:49:48Zoai:localhost:1/32721Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-02-04T11:49:48Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa
Efeito citotóxicos e genotóxicos de extratos Solanum lycocarpum St.-Hil (Solanaceae) no ciclo celular de Lactuca sativa e Allium cepa
title Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa
spellingShingle Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa
Chiavegatto, Raquel Bezerra
Aneugenic
Clastogenic
Medicinal plant
Aneugênico
Cerrado
Clastogênico
Lobeira
Planta medicinal
title_short Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa
title_full Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa
title_fullStr Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa
title_full_unstemmed Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa
title_sort Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa
author Chiavegatto, Raquel Bezerra
author_facet Chiavegatto, Raquel Bezerra
Chaves, Ana Luisa Arantes
Silva, Izabela Caputo Assis
Lima, Luciana Alves Rodrigues dos Santos
Techio, Vânia Helena
author_role author
author2 Chaves, Ana Luisa Arantes
Silva, Izabela Caputo Assis
Lima, Luciana Alves Rodrigues dos Santos
Techio, Vânia Helena
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Chiavegatto, Raquel Bezerra
Chaves, Ana Luisa Arantes
Silva, Izabela Caputo Assis
Lima, Luciana Alves Rodrigues dos Santos
Techio, Vânia Helena
dc.subject.por.fl_str_mv Aneugenic
Clastogenic
Medicinal plant
Aneugênico
Cerrado
Clastogênico
Lobeira
Planta medicinal
topic Aneugenic
Clastogenic
Medicinal plant
Aneugênico
Cerrado
Clastogênico
Lobeira
Planta medicinal
description Solanum lycocarpum St.-Hil popularly known as ‘fruta-de-lobo’ or ‘lobeira’ is native to the Brazilian Cerrado, and used in folk medicine due to its phytotherapic properties. The action of S. lycocarpumon the cell cycle and chromosomes in order to demonstrate whether there are aneugenic and/or clastogenic effects is unknown. Thus, this study aimed at investigating the cytotoxic and genotoxic potential of methanol and hexane extracts of S. lycocarpumon growth and cell cycle of Lactuca sativaand Allium cepa. Roots from both species were exposed for 72 hours to methanol and hexane extracts with 50, 100, and 200 μg mL-1 of S. lycocarpum. Slides were prepared by the squash technique and then analyzed to determine the mitotic index and the total of chromosomal and nuclear abnormalities. The frequencies of chromosomal and nuclear abnormalities were high and significant with a dose-dependent effect, indicating that S. lycocarpumhas a cytotoxic and genotoxic action depending on the dose used on meristem cells of A. cepaand L. sativa.
publishDate 2017
dc.date.none.fl_str_mv 2017
2019-02-04T11:49:48Z
2019-02-04T11:49:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv CHIAVEGATTO, R. B. et al. Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa. Acta Scientiarum. Biological Sciences, Maringá, v. 39, n. 2, p. 201-210, 2017.
http://repositorio.ufla.br/jspui/handle/1/32721
identifier_str_mv CHIAVEGATTO, R. B. et al. Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa. Acta Scientiarum. Biological Sciences, Maringá, v. 39, n. 2, p. 201-210, 2017.
url http://repositorio.ufla.br/jspui/handle/1/32721
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Editora da Universidade Estadual de Maringá (Eduem)
publisher.none.fl_str_mv Editora da Universidade Estadual de Maringá (Eduem)
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences
reponame:Repositório Institucional da UFLA
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instname_str Universidade Federal de Lavras (UFLA)
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reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
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