Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34082 |
Resumo: | Solanum lycocarpum St.-Hil popularly known as ‘fruta-de-lobo’ or ‘lobeira’ is native to the Brazilian Cerrado, and used in folk medicine due to its phytotherapic properties. The action of S. lycocarpum on the cell cycle and chromosomes in order to demonstrate whether there are aneugenic and/or clastogenic effects is unknown. Thus, this study aimed at investigating the cytotoxic and genotoxic potential of methanol and hexane extracts of S. lycocarpum on growth and cell cycle of Lactuca sativa and Allium cepa. Roots from both species were exposed for 72 hours to methanol and hexane extracts with 50, 100, and 200 µg mL-1 of S. lycocarpum. Slides were prepared by the squash technique and then analyzed to determine the mitotic index and the total of chromosomal and nuclear abnormalities. The frequencies of chromosomal and nuclear abnormalities were high and significant with a dose-dependent effect, indicating that S. lycocarpum has a cytotoxic and genotoxic action depending on the dose used on meristem cells of A. cepa and L. sativa. |
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Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepaaneugenicCerradoclastogeniclobeiramedicinal plant.Citologia Solanum lycocarpum St.-Hil popularly known as ‘fruta-de-lobo’ or ‘lobeira’ is native to the Brazilian Cerrado, and used in folk medicine due to its phytotherapic properties. The action of S. lycocarpum on the cell cycle and chromosomes in order to demonstrate whether there are aneugenic and/or clastogenic effects is unknown. Thus, this study aimed at investigating the cytotoxic and genotoxic potential of methanol and hexane extracts of S. lycocarpum on growth and cell cycle of Lactuca sativa and Allium cepa. Roots from both species were exposed for 72 hours to methanol and hexane extracts with 50, 100, and 200 µg mL-1 of S. lycocarpum. Slides were prepared by the squash technique and then analyzed to determine the mitotic index and the total of chromosomal and nuclear abnormalities. The frequencies of chromosomal and nuclear abnormalities were high and significant with a dose-dependent effect, indicating that S. lycocarpum has a cytotoxic and genotoxic action depending on the dose used on meristem cells of A. cepa and L. sativa. Universidade Estadual De Maringá2017-06-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3408210.4025/actascibiolsci.v39i2.34082Acta Scientiarum. Biological Sciences; Vol 39 No 2 (2017); 201-210Acta Scientiarum. Biological Sciences; v. 39 n. 2 (2017); 201-2101807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34082/pdfCopyright (c) 2017 Acta Scientiarum. Biological Sciencesinfo:eu-repo/semantics/openAccessChiavegatto, Raquel BezerraChaves, Ana Luisa ArantesSilva, Izabela Caputo AssisLima, Luciana Alves Rodrigues dos SantosTechio, Vânia Helena2022-02-17T22:00:22Zoai:periodicos.uem.br/ojs:article/34082Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-17T22:00:22Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa |
title |
Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa |
spellingShingle |
Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa Chiavegatto, Raquel Bezerra aneugenic Cerrado clastogenic lobeira medicinal plant. Citologia |
title_short |
Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa |
title_full |
Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa |
title_fullStr |
Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa |
title_full_unstemmed |
Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa |
title_sort |
Cytotoxic and genotoxic effects of Solanum lycocarpum St.-Hil (Solanaceae) on the cell cycle of Lactuca sativa and Allium cepa |
author |
Chiavegatto, Raquel Bezerra |
author_facet |
Chiavegatto, Raquel Bezerra Chaves, Ana Luisa Arantes Silva, Izabela Caputo Assis Lima, Luciana Alves Rodrigues dos Santos Techio, Vânia Helena |
author_role |
author |
author2 |
Chaves, Ana Luisa Arantes Silva, Izabela Caputo Assis Lima, Luciana Alves Rodrigues dos Santos Techio, Vânia Helena |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Chiavegatto, Raquel Bezerra Chaves, Ana Luisa Arantes Silva, Izabela Caputo Assis Lima, Luciana Alves Rodrigues dos Santos Techio, Vânia Helena |
dc.subject.por.fl_str_mv |
aneugenic Cerrado clastogenic lobeira medicinal plant. Citologia |
topic |
aneugenic Cerrado clastogenic lobeira medicinal plant. Citologia |
description |
Solanum lycocarpum St.-Hil popularly known as ‘fruta-de-lobo’ or ‘lobeira’ is native to the Brazilian Cerrado, and used in folk medicine due to its phytotherapic properties. The action of S. lycocarpum on the cell cycle and chromosomes in order to demonstrate whether there are aneugenic and/or clastogenic effects is unknown. Thus, this study aimed at investigating the cytotoxic and genotoxic potential of methanol and hexane extracts of S. lycocarpum on growth and cell cycle of Lactuca sativa and Allium cepa. Roots from both species were exposed for 72 hours to methanol and hexane extracts with 50, 100, and 200 µg mL-1 of S. lycocarpum. Slides were prepared by the squash technique and then analyzed to determine the mitotic index and the total of chromosomal and nuclear abnormalities. The frequencies of chromosomal and nuclear abnormalities were high and significant with a dose-dependent effect, indicating that S. lycocarpum has a cytotoxic and genotoxic action depending on the dose used on meristem cells of A. cepa and L. sativa. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34082 10.4025/actascibiolsci.v39i2.34082 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34082 |
identifier_str_mv |
10.4025/actascibiolsci.v39i2.34082 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34082/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Acta Scientiarum. Biological Sciences info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Acta Scientiarum. Biological Sciences |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 39 No 2 (2017); 201-210 Acta Scientiarum. Biological Sciences; v. 39 n. 2 (2017); 201-210 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317396844969984 |