Tribulus terrestrisfruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activities

Detalhes bibliográficos
Autor(a) principal: Oliveira, Nelma Neylanne Pinho Muniz
Data de Publicação: 2022
Outros Autores: Pereira, Aline Carvalho, Aazza, Smail, Germano, Carolina Mesquita, Assis, Rafael Marlon Alves de, Mendonça, Simony Carvalho, Carvalho, Alexandre Alves de, Pinto, José Eduardo Brasil Pereira, Bertolucci, Suzan Kelly Vilela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/55913
Resumo: Tribulus terrestris fruit has been used as a traditional and popular medicine for the prevention and treatment of several diseases, including sexual dysfunction, atherosclerosis and hypertension. The aim of this study was to evaluate the antioxidant, lipoxygenase and α-glucosidase inhibitory activities of an ethanol extract from Brazilian T. terrestris and its fractions sequentially partitioned into n-hexane, dichloromethane, ethyl acetate and n-butanol. The antioxidant capacities were determined by DPPH and ABTS scavenging free radicals, chelating metal ions, reducing power and total antioxidant activity by using phosphomolybdenum. High-performance liquid chromatography with diode array detection (HPLC-DAD) fingerprint analysis and quantitation of total phenolics were performed on the samples. The dichloromethane fraction showed the most complex HPLC-DAD chemical profile. The ethyl acetate and butanol fractions revealed the best phenolic compound and flavonoid recovery from T. terrestris. Concerning antioxidant activity, the ethyl acetate fraction presented better capacity for scavenging DPPH, ABTS and hydroxyl radicals, reductive power, total antioxidant capacity (TAC) and α-glucosidase inhibitory activity than the other fractions. These results correlated closely with the levels of phenolic compounds and flavonoids. The hexane fraction showed the best metal chelating power and lipoxygenase inhibitory activity. The anti-diabetic and anti-inflammatory potential of Brazilian Tribulus terrestris depend on the method of preparation.
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spelling Tribulus terrestrisfruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activitiesPreparações de frutas Tribulus terrestris afetam os perfis químicos de HPLC e as atividades antioxidantes, lipoxigenase e inibidoras de α-glucosidaseLas preparaciones de Tribulus terrestris afectan los perfiles químicos de HPLC y las actividades inhibidoras de antioxidantes, lipoxigenasa y α-glucosidasaZygophyllaceaeHerbal extractsTotal phenolic contentsHPLCHigh-performance liquid chromatography (HPLC)Extratos vegetaisTeores de fenólicos totaisCLAECromatografia líquida de alta eficiênciaExtractos de plantasContenido fenólico totalTribulus terrestris fruit has been used as a traditional and popular medicine for the prevention and treatment of several diseases, including sexual dysfunction, atherosclerosis and hypertension. The aim of this study was to evaluate the antioxidant, lipoxygenase and α-glucosidase inhibitory activities of an ethanol extract from Brazilian T. terrestris and its fractions sequentially partitioned into n-hexane, dichloromethane, ethyl acetate and n-butanol. The antioxidant capacities were determined by DPPH and ABTS scavenging free radicals, chelating metal ions, reducing power and total antioxidant activity by using phosphomolybdenum. High-performance liquid chromatography with diode array detection (HPLC-DAD) fingerprint analysis and quantitation of total phenolics were performed on the samples. The dichloromethane fraction showed the most complex HPLC-DAD chemical profile. The ethyl acetate and butanol fractions revealed the best phenolic compound and flavonoid recovery from T. terrestris. Concerning antioxidant activity, the ethyl acetate fraction presented better capacity for scavenging DPPH, ABTS and hydroxyl radicals, reductive power, total antioxidant capacity (TAC) and α-glucosidase inhibitory activity than the other fractions. These results correlated closely with the levels of phenolic compounds and flavonoids. The hexane fraction showed the best metal chelating power and lipoxygenase inhibitory activity. The anti-diabetic and anti-inflammatory potential of Brazilian Tribulus terrestris depend on the method of preparation.O fruto do Tribulus terrestris tem sido utilizado como medicamento tradicional e popular para a prevenção e tratamento de diversas doenças, incluindo disfunção sexual, aterosclerose e hipertensão. O objetivo deste estudo foi avaliar as atividades antioxidante, inibitória da lipoxigenase e da α-glicosidase de um extrato etanólico de T. terrestris brasileiro e suas frações sequencialmente particionadas em n-hexano, diclorometano, acetato de etila e n-butanol. As capacidades antioxidantes foram determinadas por DPPH e ABTS sequestrando radicais livres, quelando íons metálicos, reduzindo o poder e a atividade antioxidante total usando fosfomolibdênio. Análise de impressão digital por Cromatografia Líquida de Alta Eficiência-Detector de arranjo de Diodos (CLAE-DAD)e quantificação de compostos fenólicos totais foram realizadas nas amostras. A fração diclorometano apresentou o perfil químico HPLC-DAD mais complexo. As frações acetato de etila e butanol revelaram a melhor recuperação de compostos fenólicos e flavonoides de T. terrestris. Com relação à atividade antioxidante, a fração acetato de etila apresentou melhor capacidade de sequestro de DPPH, ABTS e radicais hidroxila, poder redutor, capacidade antioxidante total (TAC) e atividade inibitória da α-glicosidasedo que as outras frações. Esses resultados correlacionaram-se estreitamente com os níveis de compostos fenólicos e flavonóides. A fração hexânica apresentou o melhor poder quelante de metais e atividade inibitória da lipoxigenase. O potencial antidiabético e antiinflamatório do T. terrestris brasileiro depende do modo de preparo.Universidade Federal de Itajubá (UNIFEI), Campus Itabira2023-01-31T16:48:18Z2023-01-31T16:48:18Z2022-12-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfOLIVEIRA, N. N. P. M. et al. Tribulus terrestris fruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activities. Research, Society and Development, [S.l.], v. 11, n. 17, p. 1-13, 2022. DOI: 10.33448/rsd-v11i17.38751.http://repositorio.ufla.br/jspui/handle/1/55913Research, Society and Developmentreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessOliveira, Nelma Neylanne Pinho MunizPereira, Aline CarvalhoAazza, SmailGermano, Carolina MesquitaAssis, Rafael Marlon Alves deMendonça, Simony CarvalhoCarvalho, Alexandre Alves dePinto, José Eduardo Brasil PereiraBertolucci, Suzan Kelly Vilelaeng2023-05-26T18:57:48Zoai:localhost:1/55913Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:57:48Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Tribulus terrestrisfruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activities
Preparações de frutas Tribulus terrestris afetam os perfis químicos de HPLC e as atividades antioxidantes, lipoxigenase e inibidoras de α-glucosidase
Las preparaciones de Tribulus terrestris afectan los perfiles químicos de HPLC y las actividades inhibidoras de antioxidantes, lipoxigenasa y α-glucosidasa
title Tribulus terrestrisfruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activities
spellingShingle Tribulus terrestrisfruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activities
Oliveira, Nelma Neylanne Pinho Muniz
Zygophyllaceae
Herbal extracts
Total phenolic contents
HPLC
High-performance liquid chromatography (HPLC)
Extratos vegetais
Teores de fenólicos totais
CLAE
Cromatografia líquida de alta eficiência
Extractos de plantas
Contenido fenólico total
title_short Tribulus terrestrisfruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activities
title_full Tribulus terrestrisfruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activities
title_fullStr Tribulus terrestrisfruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activities
title_full_unstemmed Tribulus terrestrisfruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activities
title_sort Tribulus terrestrisfruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activities
author Oliveira, Nelma Neylanne Pinho Muniz
author_facet Oliveira, Nelma Neylanne Pinho Muniz
Pereira, Aline Carvalho
Aazza, Smail
Germano, Carolina Mesquita
Assis, Rafael Marlon Alves de
Mendonça, Simony Carvalho
Carvalho, Alexandre Alves de
Pinto, José Eduardo Brasil Pereira
Bertolucci, Suzan Kelly Vilela
author_role author
author2 Pereira, Aline Carvalho
Aazza, Smail
Germano, Carolina Mesquita
Assis, Rafael Marlon Alves de
Mendonça, Simony Carvalho
Carvalho, Alexandre Alves de
Pinto, José Eduardo Brasil Pereira
Bertolucci, Suzan Kelly Vilela
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Nelma Neylanne Pinho Muniz
Pereira, Aline Carvalho
Aazza, Smail
Germano, Carolina Mesquita
Assis, Rafael Marlon Alves de
Mendonça, Simony Carvalho
Carvalho, Alexandre Alves de
Pinto, José Eduardo Brasil Pereira
Bertolucci, Suzan Kelly Vilela
dc.subject.por.fl_str_mv Zygophyllaceae
Herbal extracts
Total phenolic contents
HPLC
High-performance liquid chromatography (HPLC)
Extratos vegetais
Teores de fenólicos totais
CLAE
Cromatografia líquida de alta eficiência
Extractos de plantas
Contenido fenólico total
topic Zygophyllaceae
Herbal extracts
Total phenolic contents
HPLC
High-performance liquid chromatography (HPLC)
Extratos vegetais
Teores de fenólicos totais
CLAE
Cromatografia líquida de alta eficiência
Extractos de plantas
Contenido fenólico total
description Tribulus terrestris fruit has been used as a traditional and popular medicine for the prevention and treatment of several diseases, including sexual dysfunction, atherosclerosis and hypertension. The aim of this study was to evaluate the antioxidant, lipoxygenase and α-glucosidase inhibitory activities of an ethanol extract from Brazilian T. terrestris and its fractions sequentially partitioned into n-hexane, dichloromethane, ethyl acetate and n-butanol. The antioxidant capacities were determined by DPPH and ABTS scavenging free radicals, chelating metal ions, reducing power and total antioxidant activity by using phosphomolybdenum. High-performance liquid chromatography with diode array detection (HPLC-DAD) fingerprint analysis and quantitation of total phenolics were performed on the samples. The dichloromethane fraction showed the most complex HPLC-DAD chemical profile. The ethyl acetate and butanol fractions revealed the best phenolic compound and flavonoid recovery from T. terrestris. Concerning antioxidant activity, the ethyl acetate fraction presented better capacity for scavenging DPPH, ABTS and hydroxyl radicals, reductive power, total antioxidant capacity (TAC) and α-glucosidase inhibitory activity than the other fractions. These results correlated closely with the levels of phenolic compounds and flavonoids. The hexane fraction showed the best metal chelating power and lipoxygenase inhibitory activity. The anti-diabetic and anti-inflammatory potential of Brazilian Tribulus terrestris depend on the method of preparation.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-19
2023-01-31T16:48:18Z
2023-01-31T16:48:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv OLIVEIRA, N. N. P. M. et al. Tribulus terrestris fruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activities. Research, Society and Development, [S.l.], v. 11, n. 17, p. 1-13, 2022. DOI: 10.33448/rsd-v11i17.38751.
http://repositorio.ufla.br/jspui/handle/1/55913
identifier_str_mv OLIVEIRA, N. N. P. M. et al. Tribulus terrestris fruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activities. Research, Society and Development, [S.l.], v. 11, n. 17, p. 1-13, 2022. DOI: 10.33448/rsd-v11i17.38751.
url http://repositorio.ufla.br/jspui/handle/1/55913
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Itajubá (UNIFEI), Campus Itabira
publisher.none.fl_str_mv Universidade Federal de Itajubá (UNIFEI), Campus Itabira
dc.source.none.fl_str_mv Research, Society and Development
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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