Preparações de frutas Tribulus terrestris afetam os perfis químicos de HPLC e as atividades antioxidantes, lipoxigenase e inibidoras de α-glucosidase
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/38751 |
Resumo: | Tribulus terrestris fruit has been used as a traditional and popular medicine for the prevention and treatment of several diseases, including sexual dysfunction, atherosclerosis and hypertension. The aim of this study was to evaluate the antioxidant, lipoxygenase and α-glucosidase inhibitory activities of an ethanol extract from Brazilian T. terrestris and its fractions sequentially partitioned into n-hexane, dichloromethane, ethyl acetate and n-butanol. The antioxidant capacities were determined by DPPH and ABTS scavenging free radicals, chelating metal ions, reducing power and total antioxidant activity by using phosphomolybdenum. High-performance liquid chromatography with diode array detection (HPLC-DAD) fingerprint analysis and quantitation of total phenolics were performed on the samples. The dichloromethane fraction showed the most complex HPLC-DAD chemical profile. The ethyl acetate and butanol fractions revealed the best phenolic compound and flavonoid recovery from T. terrestris. Concerning antioxidant activity, the ethyl acetate fraction presented better capacity for scavenging DPPH, ABTS and hydroxyl radicals, reductive power, total antioxidant capacity (TAC) and α-glucosidase inhibitory activity than the other fractions. These results correlated closely with the levels of phenolic compounds and flavonoids. The hexane fraction showed the best metal chelating power and lipoxygenase inhibitory activity. The anti-diabetic and anti-inflammatory potential of Brazilian Tribulus terrestris depend on the method of preparation. |
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Preparações de frutas Tribulus terrestris afetam os perfis químicos de HPLC e as atividades antioxidantes, lipoxigenase e inibidoras de α-glucosidaseLas preparaciones de Tribulus terrestris afectan los perfiles químicos de HPLC y las actividades inhibidoras de antioxidantes, lipoxigenasa y α-glucosidasaTribulus terrestris fruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activitiesZygophyllaceaeHerbal extractsTotal phenolic contentsHPLC.ZygophyllaceaeExtractos de plantasContenido fenólico totalHPLC.ZygophyllaceaeExtratos vegetaisTeores de fenólicos totaisCLAE.Tribulus terrestris fruit has been used as a traditional and popular medicine for the prevention and treatment of several diseases, including sexual dysfunction, atherosclerosis and hypertension. The aim of this study was to evaluate the antioxidant, lipoxygenase and α-glucosidase inhibitory activities of an ethanol extract from Brazilian T. terrestris and its fractions sequentially partitioned into n-hexane, dichloromethane, ethyl acetate and n-butanol. The antioxidant capacities were determined by DPPH and ABTS scavenging free radicals, chelating metal ions, reducing power and total antioxidant activity by using phosphomolybdenum. High-performance liquid chromatography with diode array detection (HPLC-DAD) fingerprint analysis and quantitation of total phenolics were performed on the samples. The dichloromethane fraction showed the most complex HPLC-DAD chemical profile. The ethyl acetate and butanol fractions revealed the best phenolic compound and flavonoid recovery from T. terrestris. Concerning antioxidant activity, the ethyl acetate fraction presented better capacity for scavenging DPPH, ABTS and hydroxyl radicals, reductive power, total antioxidant capacity (TAC) and α-glucosidase inhibitory activity than the other fractions. These results correlated closely with the levels of phenolic compounds and flavonoids. The hexane fraction showed the best metal chelating power and lipoxygenase inhibitory activity. The anti-diabetic and anti-inflammatory potential of Brazilian Tribulus terrestris depend on the method of preparation.El fruto de Tribulus terrestris ha sido utilizado como medicina tradicional y popular para la prevención y tratamiento de diversas enfermedades, entre ellas la disfunción sexual, la aterosclerosis y la hipertensión. El objetivo de este estudio fue evaluar las actividades inhibidoras de antioxidantes, lipoxigenasas y α-glucosidasas de un extracto etanólico de T. terrestris brasileño y sus fracciones divididas secuencialmente en n-hexano, diclorometano, acetato de etilo y n-butanol. Las capacidades antioxidantes se determinaron mediante DPPH y ABTS eliminando radicales libres, quelando iones metálicos, reduciendo el poder y la actividad antioxidante total utilizando fosfomolibdeno. Se realizaron análisis de huellas dactilares por Cromatografía líquida de alta resolución - detector de matriz de diodos (HPLC-DAD) y cuantificación de compuestos fenólicos totales en las muestras. La fracción de diclorometano mostró el perfil químico HPLC-DAD más complejo. Las fracciones de acetato de etilo y butanol revelaron la mejor recuperación de compuestos fenólicos y flavonoides de T. terrestris. En cuanto a la actividad antioxidante, la fracción de acetato de etilo mostró mejor capacidad de captación de radicales DPPH, ABTS e hidroxilo, poder reductor, capacidad antioxidante total (TAC) y actividad inhibitoria de la α-glucosidasa que las demás fracciones. Estos resultados se correlacionaron estrechamente con los niveles de compuestos fenólicos y flavonoides. La fracción de hexano mostró el mejor poder quelante de metales y actividad inhibidora de la lipoxigenasa. El potencial antidiabético y antiinflamatorio de la T. terrestris brasileña depende de cómo se prepare.O fruto do Tribulus terrestris tem sido utilizado como medicamento tradicional e popular para a prevenção e tratamento de diversas doenças, incluindo disfunção sexual, aterosclerose e hipertensão. O objetivo deste estudo foi avaliar as atividades antioxidante, inibitória da lipoxigenase e da α-glicosidase de um extrato etanólico de T. terrestris brasileiro e suas frações sequencialmente particionadas em n-hexano, diclorometano, acetato de etila e n-butanol. As capacidades antioxidantes foram determinadas por DPPH e ABTS sequestrando radicais livres, quelando íons metálicos, reduzindo o poder e a atividade antioxidante total usando fosfomolibdênio. Análise de impressão digital por Cromatografia Líquida de Alta Eficiência - Detector de arranjo de Diodos (CLAE-DAD) e quantificação de compostos fenólicos totais foram realizadas nas amostras. A fração diclorometano apresentou o perfil químico HPLC-DAD mais complexo. As frações acetato de etila e butanol revelaram a melhor recuperação de compostos fenólicos e flavonoides de T. terrestris. Com relação à atividade antioxidante, a fração acetato de etila apresentou melhor capacidade de sequestro de DPPH, ABTS e radicais hidroxila, poder redutor, capacidade antioxidante total (TAC) e atividade inibitória da α-glicosidase do que as outras frações. Esses resultados correlacionaram-se estreitamente com os níveis de compostos fenólicos e flavonóides. A fração hexânica apresentou o melhor poder quelante de metais e atividade inibitória da lipoxigenase. O potencial antidiabético e antiinflamatório do T. terrestris brasileiro depende do modo de preparo.Research, Society and Development2022-12-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3875110.33448/rsd-v11i17.38751Research, Society and Development; Vol. 11 No. 17; e17111738751Research, Society and Development; Vol. 11 Núm. 17; e17111738751Research, Society and Development; v. 11 n. 17; e171117387512525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/38751/32018Copyright (c) 2022 Nelma Neylanne Pinho Muniz Oliveira; Aline Carvalho Pereira; Smail Aazza; Carolina Mesquita Germano; Rafael Marlon Alves de Assis; Simony Carvalho Mendonça; Alexandre Alves de Carvalho; José Eduardo Brasil Pereira Pinto; Suzan Kelly Vilela Bertoluccihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOliveira, Nelma Neylanne Pinho Muniz Pereira, Aline Carvalho Aazza, Smail Germano, Carolina Mesquita Assis, Rafael Marlon Alves de Mendonça, Simony Carvalho Carvalho, Alexandre Alves de Pinto, José Eduardo Brasil Pereira Bertolucci, Suzan Kelly Vilela 2022-12-28T13:53:48Zoai:ojs.pkp.sfu.ca:article/38751Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:52:20.525436Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Preparações de frutas Tribulus terrestris afetam os perfis químicos de HPLC e as atividades antioxidantes, lipoxigenase e inibidoras de α-glucosidase Las preparaciones de Tribulus terrestris afectan los perfiles químicos de HPLC y las actividades inhibidoras de antioxidantes, lipoxigenasa y α-glucosidasa Tribulus terrestris fruit preparations impact HPLC chemical profiles and antioxidant, lipoxygenase and α-glucosidase inhibitory activities |
| title |
Preparações de frutas Tribulus terrestris afetam os perfis químicos de HPLC e as atividades antioxidantes, lipoxigenase e inibidoras de α-glucosidase |
| spellingShingle |
Preparações de frutas Tribulus terrestris afetam os perfis químicos de HPLC e as atividades antioxidantes, lipoxigenase e inibidoras de α-glucosidase Oliveira, Nelma Neylanne Pinho Muniz Zygophyllaceae Herbal extracts Total phenolic contents HPLC. Zygophyllaceae Extractos de plantas Contenido fenólico total HPLC. Zygophyllaceae Extratos vegetais Teores de fenólicos totais CLAE. |
| title_short |
Preparações de frutas Tribulus terrestris afetam os perfis químicos de HPLC e as atividades antioxidantes, lipoxigenase e inibidoras de α-glucosidase |
| title_full |
Preparações de frutas Tribulus terrestris afetam os perfis químicos de HPLC e as atividades antioxidantes, lipoxigenase e inibidoras de α-glucosidase |
| title_fullStr |
Preparações de frutas Tribulus terrestris afetam os perfis químicos de HPLC e as atividades antioxidantes, lipoxigenase e inibidoras de α-glucosidase |
| title_full_unstemmed |
Preparações de frutas Tribulus terrestris afetam os perfis químicos de HPLC e as atividades antioxidantes, lipoxigenase e inibidoras de α-glucosidase |
| title_sort |
Preparações de frutas Tribulus terrestris afetam os perfis químicos de HPLC e as atividades antioxidantes, lipoxigenase e inibidoras de α-glucosidase |
| author |
Oliveira, Nelma Neylanne Pinho Muniz |
| author_facet |
Oliveira, Nelma Neylanne Pinho Muniz Pereira, Aline Carvalho Aazza, Smail Germano, Carolina Mesquita Assis, Rafael Marlon Alves de Mendonça, Simony Carvalho Carvalho, Alexandre Alves de Pinto, José Eduardo Brasil Pereira Bertolucci, Suzan Kelly Vilela |
| author_role |
author |
| author2 |
Pereira, Aline Carvalho Aazza, Smail Germano, Carolina Mesquita Assis, Rafael Marlon Alves de Mendonça, Simony Carvalho Carvalho, Alexandre Alves de Pinto, José Eduardo Brasil Pereira Bertolucci, Suzan Kelly Vilela |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Nelma Neylanne Pinho Muniz Pereira, Aline Carvalho Aazza, Smail Germano, Carolina Mesquita Assis, Rafael Marlon Alves de Mendonça, Simony Carvalho Carvalho, Alexandre Alves de Pinto, José Eduardo Brasil Pereira Bertolucci, Suzan Kelly Vilela |
| dc.subject.por.fl_str_mv |
Zygophyllaceae Herbal extracts Total phenolic contents HPLC. Zygophyllaceae Extractos de plantas Contenido fenólico total HPLC. Zygophyllaceae Extratos vegetais Teores de fenólicos totais CLAE. |
| topic |
Zygophyllaceae Herbal extracts Total phenolic contents HPLC. Zygophyllaceae Extractos de plantas Contenido fenólico total HPLC. Zygophyllaceae Extratos vegetais Teores de fenólicos totais CLAE. |
| description |
Tribulus terrestris fruit has been used as a traditional and popular medicine for the prevention and treatment of several diseases, including sexual dysfunction, atherosclerosis and hypertension. The aim of this study was to evaluate the antioxidant, lipoxygenase and α-glucosidase inhibitory activities of an ethanol extract from Brazilian T. terrestris and its fractions sequentially partitioned into n-hexane, dichloromethane, ethyl acetate and n-butanol. The antioxidant capacities were determined by DPPH and ABTS scavenging free radicals, chelating metal ions, reducing power and total antioxidant activity by using phosphomolybdenum. High-performance liquid chromatography with diode array detection (HPLC-DAD) fingerprint analysis and quantitation of total phenolics were performed on the samples. The dichloromethane fraction showed the most complex HPLC-DAD chemical profile. The ethyl acetate and butanol fractions revealed the best phenolic compound and flavonoid recovery from T. terrestris. Concerning antioxidant activity, the ethyl acetate fraction presented better capacity for scavenging DPPH, ABTS and hydroxyl radicals, reductive power, total antioxidant capacity (TAC) and α-glucosidase inhibitory activity than the other fractions. These results correlated closely with the levels of phenolic compounds and flavonoids. The hexane fraction showed the best metal chelating power and lipoxygenase inhibitory activity. The anti-diabetic and anti-inflammatory potential of Brazilian Tribulus terrestris depend on the method of preparation. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-12-19 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/38751 10.33448/rsd-v11i17.38751 |
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https://rsdjournal.org/index.php/rsd/article/view/38751 |
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10.33448/rsd-v11i17.38751 |
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por |
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por |
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https://rsdjournal.org/index.php/rsd/article/view/38751/32018 |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 11 No. 17; e17111738751 Research, Society and Development; Vol. 11 Núm. 17; e17111738751 Research, Society and Development; v. 11 n. 17; e17111738751 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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