Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes)
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542007000400016 http://repositorio.ufla.br/jspui/handle/1/6465 |
Resumo: | The mangabeira presents potential for fruit and rubber production. Since the seeds are recalcitrant which makes difficult its propagation, new approaches are needed in order to obtain plants through asexual methods. In this context, the process of in vitro culture presents as an alternative for the mangabeira propagation. The objective of the present work was to establish a micropropagation methodology of mangabeira through direct organogenesis. To obtain shoots, nodal segments collected from in vitro germinated seedlings were inoculated in WPM medium supplemented with different concentrations of BAP (0.0; 1.0; 2.0; 3.0; 4.0 e 5.0 mg L-1). For root induction, shoots were inoculated in WPM medium supplemented with 0.1% activated charcoal and different concentrations of NAA (0.0; 1.0; 2.0 e 3.0 mg L-1) or IBA (0.0; 1.0; 2.0; 3.0 e 4.0 mg L-1). The inoculated shoots were maintained in for 15 days a growth room after which were transferred to a free grow regulator WPM medium. The concentrations of 5.0 mg L-1 and 3.0 mg L-1 BAP were the most eficient for shoots and buds induction, respectively. Higher shoot length was observed in medium suplemented with 1.0 mg L-1 or 2,0 mg L-1 BAP. The higher callus formation occurred in medium suplemented with 4.0 mg L-1 BAP. The use of NAA was not efficient, in all tested concentrations, to induce in vitro root formation in shoots. In presence of 3.0 mg L-1 IBA, 20% of the shoots developed roots. |
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Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes)Direct organogenesis in nodal explants of mangabeira (Hancornia speciosa Gomes)MicropropagaçãoBrotaçõesEnraizamentoMicropropagationShootingRootingThe mangabeira presents potential for fruit and rubber production. Since the seeds are recalcitrant which makes difficult its propagation, new approaches are needed in order to obtain plants through asexual methods. In this context, the process of in vitro culture presents as an alternative for the mangabeira propagation. The objective of the present work was to establish a micropropagation methodology of mangabeira through direct organogenesis. To obtain shoots, nodal segments collected from in vitro germinated seedlings were inoculated in WPM medium supplemented with different concentrations of BAP (0.0; 1.0; 2.0; 3.0; 4.0 e 5.0 mg L-1). For root induction, shoots were inoculated in WPM medium supplemented with 0.1% activated charcoal and different concentrations of NAA (0.0; 1.0; 2.0 e 3.0 mg L-1) or IBA (0.0; 1.0; 2.0; 3.0 e 4.0 mg L-1). The inoculated shoots were maintained in for 15 days a growth room after which were transferred to a free grow regulator WPM medium. The concentrations of 5.0 mg L-1 and 3.0 mg L-1 BAP were the most eficient for shoots and buds induction, respectively. Higher shoot length was observed in medium suplemented with 1.0 mg L-1 or 2,0 mg L-1 BAP. The higher callus formation occurred in medium suplemented with 4.0 mg L-1 BAP. The use of NAA was not efficient, in all tested concentrations, to induce in vitro root formation in shoots. In presence of 3.0 mg L-1 IBA, 20% of the shoots developed roots.A mangabeira, espécie nativa do Cerrado, destaca-se por possuir um grande potencial como planta frutífera e produtora de borracha. No entanto, suas sementes apresentam recalcitrância, dificultando sua propagação, o que torna evidente a necessidade da obtenção de mudas por via assexuada. Neste contexto, o cultivo in vitro apresenta-se como uma alternativa a ser utilizada. Com o presente trabalho, visou-se estabelecer uma metodologia de micropropagação da mangabeira via organogênese direta. Para a obtenção de brotações, segmentos caulinares com até duas gemas laterais foram inoculados em meio de cultura WPM, suplementado com diferentes concentrações de BAP (0,0; 1,0; 2,0; 3,0; 4,0 e 5,0 mg L-1). Para o enraizamento, brotações foram inoculadas em meio WPM, suplementado com 0,1% de carvão ativado e diferentes concentrações de ANA (0,0; 1,0; 2,0 e 3,0 mg L-1) ou AIB (0,0; 1,0; 2,0; 3,0 e 4,0 mg L-1). As brotações inoculadas permaneceram em sala de crescimento por 15 dias, quando foram transferidas para meio WPM sem reguladores de crescimento. As concentrações de 5,0 mg L-1 e 3,0 mg L-1 de BAP foram as mais eficientes na indução, respectivamente, de brotações e gemas. O maior comprimento das brotações foi verificado em meio suplementado com 1,0 mg L-1 ou 2,0 mg L-1 de BAP. A maior formação de calos ocorreu em meio suplementado com 4,0 mg L-1 dessa citocinina. A auxina ANA não se mostrou eficiente, nas concentrações testadas, no enraizamento in vitro de brotações de mangabeira. Na presença de 3,0 mg L-1 de AIB, 20% das brotações enraizaram.Editora da Universidade Federal de Lavras2007-08-012015-04-30T13:34:15Z2015-04-30T13:34:15Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542007000400016SOARES, F. P. et al. Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes). Ciência e Agrotecnologia, Lavras, v. 31, n. 4, p. 1048-1053, jul./ago. 2007.http://repositorio.ufla.br/jspui/handle/1/6465Ciência e Agrotecnologia v.31 n.4 2007reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLASoares, Fernanda PereiraPaiva, RenatoAlvarenga, Amauri Alves deNogueira, Raírys CravoEmrich, Eduardo BucsanMartinotto, Cristianoporinfo:eu-repo/semantics/openAccess2016-07-29T12:05:47Zoai:localhost:1/6465Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-07-29T12:05:47Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes) Direct organogenesis in nodal explants of mangabeira (Hancornia speciosa Gomes) |
title |
Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes) |
spellingShingle |
Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes) Soares, Fernanda Pereira Micropropagação Brotações Enraizamento Micropropagation Shooting Rooting |
title_short |
Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes) |
title_full |
Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes) |
title_fullStr |
Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes) |
title_full_unstemmed |
Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes) |
title_sort |
Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes) |
author |
Soares, Fernanda Pereira |
author_facet |
Soares, Fernanda Pereira Paiva, Renato Alvarenga, Amauri Alves de Nogueira, Raírys Cravo Emrich, Eduardo Bucsan Martinotto, Cristiano |
author_role |
author |
author2 |
Paiva, Renato Alvarenga, Amauri Alves de Nogueira, Raírys Cravo Emrich, Eduardo Bucsan Martinotto, Cristiano |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Soares, Fernanda Pereira Paiva, Renato Alvarenga, Amauri Alves de Nogueira, Raírys Cravo Emrich, Eduardo Bucsan Martinotto, Cristiano |
dc.subject.por.fl_str_mv |
Micropropagação Brotações Enraizamento Micropropagation Shooting Rooting |
topic |
Micropropagação Brotações Enraizamento Micropropagation Shooting Rooting |
description |
The mangabeira presents potential for fruit and rubber production. Since the seeds are recalcitrant which makes difficult its propagation, new approaches are needed in order to obtain plants through asexual methods. In this context, the process of in vitro culture presents as an alternative for the mangabeira propagation. The objective of the present work was to establish a micropropagation methodology of mangabeira through direct organogenesis. To obtain shoots, nodal segments collected from in vitro germinated seedlings were inoculated in WPM medium supplemented with different concentrations of BAP (0.0; 1.0; 2.0; 3.0; 4.0 e 5.0 mg L-1). For root induction, shoots were inoculated in WPM medium supplemented with 0.1% activated charcoal and different concentrations of NAA (0.0; 1.0; 2.0 e 3.0 mg L-1) or IBA (0.0; 1.0; 2.0; 3.0 e 4.0 mg L-1). The inoculated shoots were maintained in for 15 days a growth room after which were transferred to a free grow regulator WPM medium. The concentrations of 5.0 mg L-1 and 3.0 mg L-1 BAP were the most eficient for shoots and buds induction, respectively. Higher shoot length was observed in medium suplemented with 1.0 mg L-1 or 2,0 mg L-1 BAP. The higher callus formation occurred in medium suplemented with 4.0 mg L-1 BAP. The use of NAA was not efficient, in all tested concentrations, to induce in vitro root formation in shoots. In presence of 3.0 mg L-1 IBA, 20% of the shoots developed roots. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-08-01 2015-04-30T13:34:15Z 2015-04-30T13:34:15Z 2015-04-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542007000400016 SOARES, F. P. et al. Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes). Ciência e Agrotecnologia, Lavras, v. 31, n. 4, p. 1048-1053, jul./ago. 2007. http://repositorio.ufla.br/jspui/handle/1/6465 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542007000400016 http://repositorio.ufla.br/jspui/handle/1/6465 |
identifier_str_mv |
SOARES, F. P. et al. Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes). Ciência e Agrotecnologia, Lavras, v. 31, n. 4, p. 1048-1053, jul./ago. 2007. |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.31 n.4 2007 reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
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UFLA |
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UFLA |
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Repositório Institucional da UFLA |
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Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1815439209169682432 |