Morphological characteristics and cell viability of coffee plants calli
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/28547 |
Resumo: | The aim of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá). Cells of different types of callus were successfully characterized regarding viability and internal and external morphological characteristics. It was obtained two morphologically distinct types of callus: (i) yellow friable and (ii) transparent watery. The yellow friable calli showed higher cell viability and embryogenic characteristics. Scanning and transmission electron microscopy showed embryogenic characteristics in cells of the yellow friable calli evidenced by the presence of small and isodiametric cells, while transparent watery calli showed elongated cells and large cytoplasm vacuolization. |
id |
UFLA_7b48b603c6f1cc550d2d4f2b4f1c71f3 |
---|---|
oai_identifier_str |
oai:localhost:1/28547 |
network_acronym_str |
UFLA |
network_name_str |
Repositório Institucional da UFLA |
repository_id_str |
|
spelling |
Morphological characteristics and cell viability of coffee plants calliCaracterísticas morfológicas e viabilidade celular de calos de cafeeiroCoffea arabicaElectron microscopyTetrazolium testMicroscopia eletrônicaTeste do tetrazólioThe aim of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá). Cells of different types of callus were successfully characterized regarding viability and internal and external morphological characteristics. It was obtained two morphologically distinct types of callus: (i) yellow friable and (ii) transparent watery. The yellow friable calli showed higher cell viability and embryogenic characteristics. Scanning and transmission electron microscopy showed embryogenic characteristics in cells of the yellow friable calli evidenced by the presence of small and isodiametric cells, while transparent watery calli showed elongated cells and large cytoplasm vacuolization.O objetivo deste trabalho foi caracterizar e comparar dois tipos de calos de explantes foliares de Coffea arabica (cultivar Catiguá). Células de diferentes tipos de calos foram caracterizadas quanto a viabilidade e características morfológicas externas e internas. Foram obtidos dois tipos de calos morfologicamente distintos: (a) amarelo friável e (b) transparente aquoso. Os calos amarelos friáveis apresentaram maior viabilidade celular e características embriogênicas. Microscopia eletrônica de varredura e transmissão mostraram características embriogênicas em calos amarelos friáveis evidenciadas pela presença de células pequenas e isodiamétricas. Os calos transparentes aquosos apresentaram células alongadas e vacuolizadas.Universidade Federal de Santa Maria2018-02-07T12:17:30Z2018-02-07T12:17:30Z2014-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPÁDUA, M. S. et al. Morphological characteristics and cell viability of coffee plants calli. Ciência Rural, Santa Maria, v. 44, n. 4, p. 660-665, abr. 2014.http://repositorio.ufla.br/jspui/handle/1/28547Ciência Ruralreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessPádua, Marlúcia SouzaPaiva, Luciano VilelaSilva, Luciano Coutinho daLivramento, Kalynka Gabriella doAlves, EduardoCastro, Ana Hortência Fonsecaeng2023-05-26T19:51:06Zoai:localhost:1/28547Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T19:51:06Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Morphological characteristics and cell viability of coffee plants calli Características morfológicas e viabilidade celular de calos de cafeeiro |
title |
Morphological characteristics and cell viability of coffee plants calli |
spellingShingle |
Morphological characteristics and cell viability of coffee plants calli Pádua, Marlúcia Souza Coffea arabica Electron microscopy Tetrazolium test Microscopia eletrônica Teste do tetrazólio |
title_short |
Morphological characteristics and cell viability of coffee plants calli |
title_full |
Morphological characteristics and cell viability of coffee plants calli |
title_fullStr |
Morphological characteristics and cell viability of coffee plants calli |
title_full_unstemmed |
Morphological characteristics and cell viability of coffee plants calli |
title_sort |
Morphological characteristics and cell viability of coffee plants calli |
author |
Pádua, Marlúcia Souza |
author_facet |
Pádua, Marlúcia Souza Paiva, Luciano Vilela Silva, Luciano Coutinho da Livramento, Kalynka Gabriella do Alves, Eduardo Castro, Ana Hortência Fonseca |
author_role |
author |
author2 |
Paiva, Luciano Vilela Silva, Luciano Coutinho da Livramento, Kalynka Gabriella do Alves, Eduardo Castro, Ana Hortência Fonseca |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pádua, Marlúcia Souza Paiva, Luciano Vilela Silva, Luciano Coutinho da Livramento, Kalynka Gabriella do Alves, Eduardo Castro, Ana Hortência Fonseca |
dc.subject.por.fl_str_mv |
Coffea arabica Electron microscopy Tetrazolium test Microscopia eletrônica Teste do tetrazólio |
topic |
Coffea arabica Electron microscopy Tetrazolium test Microscopia eletrônica Teste do tetrazólio |
description |
The aim of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá). Cells of different types of callus were successfully characterized regarding viability and internal and external morphological characteristics. It was obtained two morphologically distinct types of callus: (i) yellow friable and (ii) transparent watery. The yellow friable calli showed higher cell viability and embryogenic characteristics. Scanning and transmission electron microscopy showed embryogenic characteristics in cells of the yellow friable calli evidenced by the presence of small and isodiametric cells, while transparent watery calli showed elongated cells and large cytoplasm vacuolization. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04 2018-02-07T12:17:30Z 2018-02-07T12:17:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PÁDUA, M. S. et al. Morphological characteristics and cell viability of coffee plants calli. Ciência Rural, Santa Maria, v. 44, n. 4, p. 660-665, abr. 2014. http://repositorio.ufla.br/jspui/handle/1/28547 |
identifier_str_mv |
PÁDUA, M. S. et al. Morphological characteristics and cell viability of coffee plants calli. Ciência Rural, Santa Maria, v. 44, n. 4, p. 660-665, abr. 2014. |
url |
http://repositorio.ufla.br/jspui/handle/1/28547 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1815439115314790400 |