Morphological characteristics and cell viability of coffee plants calli
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000400014 |
Resumo: | The aim of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá). Cells of different types of callus were successfully characterized regarding viability and internal and external morphological characteristics. It was obtained two morphologically distinct types of callus: (i) yellow friable and (ii) transparent watery. The yellow friable calli showed higher cell viability and embryogenic characteristics. Scanning and transmission electron microscopy showed embryogenic characteristics in cells of the yellow friable calli evidenced by the presence of small and isodiametric cells, while transparent watery calli showed elongated cells and large cytoplasm vacuolization. |
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Morphological characteristics and cell viability of coffee plants calliCoffea arabicaelectron microscopytetrazolium testThe aim of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá). Cells of different types of callus were successfully characterized regarding viability and internal and external morphological characteristics. It was obtained two morphologically distinct types of callus: (i) yellow friable and (ii) transparent watery. The yellow friable calli showed higher cell viability and embryogenic characteristics. Scanning and transmission electron microscopy showed embryogenic characteristics in cells of the yellow friable calli evidenced by the presence of small and isodiametric cells, while transparent watery calli showed elongated cells and large cytoplasm vacuolization.Universidade Federal de Santa Maria2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000400014Ciência Rural v.44 n.4 2014reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782014000400014info:eu-repo/semantics/openAccessPádua,Marlúcia SouzaPaiva,Luciano VilelaSilva,Luciano Coutinho daLivramento,Kalynka Gabriella doAlves,EduardoCastro,Ana Hortência Fonsecaeng2014-04-01T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Morphological characteristics and cell viability of coffee plants calli |
title |
Morphological characteristics and cell viability of coffee plants calli |
spellingShingle |
Morphological characteristics and cell viability of coffee plants calli Pádua,Marlúcia Souza Coffea arabica electron microscopy tetrazolium test |
title_short |
Morphological characteristics and cell viability of coffee plants calli |
title_full |
Morphological characteristics and cell viability of coffee plants calli |
title_fullStr |
Morphological characteristics and cell viability of coffee plants calli |
title_full_unstemmed |
Morphological characteristics and cell viability of coffee plants calli |
title_sort |
Morphological characteristics and cell viability of coffee plants calli |
author |
Pádua,Marlúcia Souza |
author_facet |
Pádua,Marlúcia Souza Paiva,Luciano Vilela Silva,Luciano Coutinho da Livramento,Kalynka Gabriella do Alves,Eduardo Castro,Ana Hortência Fonseca |
author_role |
author |
author2 |
Paiva,Luciano Vilela Silva,Luciano Coutinho da Livramento,Kalynka Gabriella do Alves,Eduardo Castro,Ana Hortência Fonseca |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pádua,Marlúcia Souza Paiva,Luciano Vilela Silva,Luciano Coutinho da Livramento,Kalynka Gabriella do Alves,Eduardo Castro,Ana Hortência Fonseca |
dc.subject.por.fl_str_mv |
Coffea arabica electron microscopy tetrazolium test |
topic |
Coffea arabica electron microscopy tetrazolium test |
description |
The aim of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá). Cells of different types of callus were successfully characterized regarding viability and internal and external morphological characteristics. It was obtained two morphologically distinct types of callus: (i) yellow friable and (ii) transparent watery. The yellow friable calli showed higher cell viability and embryogenic characteristics. Scanning and transmission electron microscopy showed embryogenic characteristics in cells of the yellow friable calli evidenced by the presence of small and isodiametric cells, while transparent watery calli showed elongated cells and large cytoplasm vacuolization. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000400014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000400014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-84782014000400014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.44 n.4 2014 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140545132822528 |