Impact of seed exudates on growth and biofilm formation of Bacillus amyloliquefaciensALB629 in common bean
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/33671 |
Resumo: | We aimed to unravel the events which favor the seed-rhizobacterium Bacillus amyloliquefaciens strain ALB629 (hereafter ALB629) interaction and which may interfere with the rhizobacterium colonization and growth on the spermosphere of common bean. Seed exudates from common bean were tested in vitro for ALB629 biofilm formation and bacterial growth. Furthermore, the performance of ALB629 on plant-related variables under drought stress was checked. Seed exudates (1 and 5% v/v) increased ALB629 biofilm formation. Additionally, the colony forming units for ALB629 increased both in culture and on the bean seed surface. The bean seed exudates up-regulated biofilm operons in ALB629 TasA and EpsD by ca. two and sixfold, respectively. The high-performance liquid chromatography (HPLC)-coupled with MS showed that malic acid is present as a major organic acid component in the seed exudates. Seeds treated with ALB629 and amended with malic acid resulted in seedlings with a higher bacterial concentration, induced plant drought tolerance, and promoted plant growth. We showed that seed exudates promote growth of ALB629 and malic acid was identified as a major organic acid component in the bean seed exudates. Our results also show that supplementation of ALB629 induced drought tolerance and growth in plants. The research pertaining to the biological significance of seed exudates in plant–microbe interaction is unexplored field and our work shows the importance of seed exudates in priming both growth and tolerance against abiotic stress. |
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Impact of seed exudates on growth and biofilm formation of Bacillus amyloliquefaciensALB629 in common beanSeed coatingAbiotic stressSporulationRhizobacteriaPlant–microbe interactionRevestimento de sementesEstresse abióticoEsporulaçãoRizobactériasInteração micróbio-plantaWe aimed to unravel the events which favor the seed-rhizobacterium Bacillus amyloliquefaciens strain ALB629 (hereafter ALB629) interaction and which may interfere with the rhizobacterium colonization and growth on the spermosphere of common bean. Seed exudates from common bean were tested in vitro for ALB629 biofilm formation and bacterial growth. Furthermore, the performance of ALB629 on plant-related variables under drought stress was checked. Seed exudates (1 and 5% v/v) increased ALB629 biofilm formation. Additionally, the colony forming units for ALB629 increased both in culture and on the bean seed surface. The bean seed exudates up-regulated biofilm operons in ALB629 TasA and EpsD by ca. two and sixfold, respectively. The high-performance liquid chromatography (HPLC)-coupled with MS showed that malic acid is present as a major organic acid component in the seed exudates. Seeds treated with ALB629 and amended with malic acid resulted in seedlings with a higher bacterial concentration, induced plant drought tolerance, and promoted plant growth. We showed that seed exudates promote growth of ALB629 and malic acid was identified as a major organic acid component in the bean seed exudates. Our results also show that supplementation of ALB629 induced drought tolerance and growth in plants. The research pertaining to the biological significance of seed exudates in plant–microbe interaction is unexplored field and our work shows the importance of seed exudates in priming both growth and tolerance against abiotic stress.Frontiers2019-04-23T12:23:31Z2019-04-23T12:23:31Z2018-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMARTINS, S. J. et al. Impact of seed exudates on growth and biofilm formation of Bacillus amyloliquefaciensALB629 in common bean. Frontiers in Microbiology, [S. l.], v. 8, p. 1-9, 2018. DOI: https://doi.org/10.3389/fmicb.2017.02631.http://repositorio.ufla.br/jspui/handle/1/33671Frontiers in Microbiologyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMartins, Samuel J.Medeiros, Flávio H. V.Lakshmanan, VenkatachalamBais, Harsh P.eng2019-04-23T12:23:31Zoai:localhost:1/33671Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-04-23T12:23:31Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Impact of seed exudates on growth and biofilm formation of Bacillus amyloliquefaciensALB629 in common bean |
title |
Impact of seed exudates on growth and biofilm formation of Bacillus amyloliquefaciensALB629 in common bean |
spellingShingle |
Impact of seed exudates on growth and biofilm formation of Bacillus amyloliquefaciensALB629 in common bean Martins, Samuel J. Seed coating Abiotic stress Sporulation Rhizobacteria Plant–microbe interaction Revestimento de sementes Estresse abiótico Esporulação Rizobactérias Interação micróbio-planta |
title_short |
Impact of seed exudates on growth and biofilm formation of Bacillus amyloliquefaciensALB629 in common bean |
title_full |
Impact of seed exudates on growth and biofilm formation of Bacillus amyloliquefaciensALB629 in common bean |
title_fullStr |
Impact of seed exudates on growth and biofilm formation of Bacillus amyloliquefaciensALB629 in common bean |
title_full_unstemmed |
Impact of seed exudates on growth and biofilm formation of Bacillus amyloliquefaciensALB629 in common bean |
title_sort |
Impact of seed exudates on growth and biofilm formation of Bacillus amyloliquefaciensALB629 in common bean |
author |
Martins, Samuel J. |
author_facet |
Martins, Samuel J. Medeiros, Flávio H. V. Lakshmanan, Venkatachalam Bais, Harsh P. |
author_role |
author |
author2 |
Medeiros, Flávio H. V. Lakshmanan, Venkatachalam Bais, Harsh P. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Martins, Samuel J. Medeiros, Flávio H. V. Lakshmanan, Venkatachalam Bais, Harsh P. |
dc.subject.por.fl_str_mv |
Seed coating Abiotic stress Sporulation Rhizobacteria Plant–microbe interaction Revestimento de sementes Estresse abiótico Esporulação Rizobactérias Interação micróbio-planta |
topic |
Seed coating Abiotic stress Sporulation Rhizobacteria Plant–microbe interaction Revestimento de sementes Estresse abiótico Esporulação Rizobactérias Interação micróbio-planta |
description |
We aimed to unravel the events which favor the seed-rhizobacterium Bacillus amyloliquefaciens strain ALB629 (hereafter ALB629) interaction and which may interfere with the rhizobacterium colonization and growth on the spermosphere of common bean. Seed exudates from common bean were tested in vitro for ALB629 biofilm formation and bacterial growth. Furthermore, the performance of ALB629 on plant-related variables under drought stress was checked. Seed exudates (1 and 5% v/v) increased ALB629 biofilm formation. Additionally, the colony forming units for ALB629 increased both in culture and on the bean seed surface. The bean seed exudates up-regulated biofilm operons in ALB629 TasA and EpsD by ca. two and sixfold, respectively. The high-performance liquid chromatography (HPLC)-coupled with MS showed that malic acid is present as a major organic acid component in the seed exudates. Seeds treated with ALB629 and amended with malic acid resulted in seedlings with a higher bacterial concentration, induced plant drought tolerance, and promoted plant growth. We showed that seed exudates promote growth of ALB629 and malic acid was identified as a major organic acid component in the bean seed exudates. Our results also show that supplementation of ALB629 induced drought tolerance and growth in plants. The research pertaining to the biological significance of seed exudates in plant–microbe interaction is unexplored field and our work shows the importance of seed exudates in priming both growth and tolerance against abiotic stress. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01 2019-04-23T12:23:31Z 2019-04-23T12:23:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MARTINS, S. J. et al. Impact of seed exudates on growth and biofilm formation of Bacillus amyloliquefaciensALB629 in common bean. Frontiers in Microbiology, [S. l.], v. 8, p. 1-9, 2018. DOI: https://doi.org/10.3389/fmicb.2017.02631. http://repositorio.ufla.br/jspui/handle/1/33671 |
identifier_str_mv |
MARTINS, S. J. et al. Impact of seed exudates on growth and biofilm formation of Bacillus amyloliquefaciensALB629 in common bean. Frontiers in Microbiology, [S. l.], v. 8, p. 1-9, 2018. DOI: https://doi.org/10.3389/fmicb.2017.02631. |
url |
http://repositorio.ufla.br/jspui/handle/1/33671 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers |
publisher.none.fl_str_mv |
Frontiers |
dc.source.none.fl_str_mv |
Frontiers in Microbiology reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835065889062912 |