Cultivo in vitro e criopreservação de estrelícia
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/43343 |
Resumo: | Streltzia (Strelitzia reginae) is an ornamental plant used as a cut flower and in landscaping. However, specie propagation is limited by the chemical and physical dormancy of the seeds and by the number of shoots formed, thus, in vitro propagation is an alternative for the reproduction of this species. However, in vitro produced seedlings may present limitations during acclimatization, it is necessary to develop a methodology to optimize these step. In addition, the flower trade is marked by trends, requiring the development of techniques for the conservation of germplasm. Among the techniques that can be used, cryopreservation can be used for the long-term conservation of the species, being necessary to know about the effects on the genetic stability and anatomical aspects of the plants. The objective was to evaluate how GA3 and temperature affect the in vitro development of zygotic embryos, as well as the effect of BAP and TDZ on multiplication, the use of coloured shade nets during acclimatization and the effect of cryopreservation in the development, anatomy and genetic stability of streliztia. Greater germination (72%) and shoot length (3.14 cm) were observed with the use of 20 μM of GA3. In this concentration there was an increase in nitrate reductase activity, without altering the genetic stability. The temperature influenced plant growth, which was higher at 25ºC and also in the SOD and APX activity, which were higher at 30ºC. The results showed that 20 μM BAP provided a higher shoot formation, however, these showed a reduction in length, as well as increased oxidation of the medium since TDZ did not induce shoots. During acclimatization, the highest survival rate occurred for plants maintained without coloured shade nets and during cryopreservation, silica gel dehydration for 30 minutes was efficient for better seedling development, with no anatomical changes and changes in genetic stability. So, it is recommended for the cultivation of zygotic embryos, the addition of 20 μM of GA3 and maintenance at the temperature of 25°C, and for the multiplication 20 μM of BAP is efficient and acclimatization must be performed in the absence of coloured shade nets and for cryopreservation, dehydration should be performed for 30 minutes on silica gel. |
id |
UFLA_812261b8c1c628b625921b6bcba22a8e |
---|---|
oai_identifier_str |
oai:localhost:1/43343 |
network_acronym_str |
UFLA |
network_name_str |
Repositório Institucional da UFLA |
repository_id_str |
|
spelling |
Cultivo in vitro e criopreservação de estrelíciaIn vitro culture and cryopreservation of strelitziaStrelitzia reginaeGiberelinaCitocininaAclimatizaçãoCriopreservaçãoGibberellinCytokininAcclimatizationCryopreservationFisiologia VegetalStreltzia (Strelitzia reginae) is an ornamental plant used as a cut flower and in landscaping. However, specie propagation is limited by the chemical and physical dormancy of the seeds and by the number of shoots formed, thus, in vitro propagation is an alternative for the reproduction of this species. However, in vitro produced seedlings may present limitations during acclimatization, it is necessary to develop a methodology to optimize these step. In addition, the flower trade is marked by trends, requiring the development of techniques for the conservation of germplasm. Among the techniques that can be used, cryopreservation can be used for the long-term conservation of the species, being necessary to know about the effects on the genetic stability and anatomical aspects of the plants. The objective was to evaluate how GA3 and temperature affect the in vitro development of zygotic embryos, as well as the effect of BAP and TDZ on multiplication, the use of coloured shade nets during acclimatization and the effect of cryopreservation in the development, anatomy and genetic stability of streliztia. Greater germination (72%) and shoot length (3.14 cm) were observed with the use of 20 μM of GA3. In this concentration there was an increase in nitrate reductase activity, without altering the genetic stability. The temperature influenced plant growth, which was higher at 25ºC and also in the SOD and APX activity, which were higher at 30ºC. The results showed that 20 μM BAP provided a higher shoot formation, however, these showed a reduction in length, as well as increased oxidation of the medium since TDZ did not induce shoots. During acclimatization, the highest survival rate occurred for plants maintained without coloured shade nets and during cryopreservation, silica gel dehydration for 30 minutes was efficient for better seedling development, with no anatomical changes and changes in genetic stability. So, it is recommended for the cultivation of zygotic embryos, the addition of 20 μM of GA3 and maintenance at the temperature of 25°C, and for the multiplication 20 μM of BAP is efficient and acclimatization must be performed in the absence of coloured shade nets and for cryopreservation, dehydration should be performed for 30 minutes on silica gel.A estrelícia (Strelitzia reginae) é uma planta ornamental muito utilizada como flor de corte e em jardins. No entanto, a propagação convencional da espécie é limitada pela dormência química e física das sementes e pelo pequeno número de brotos formados a partir da divisão de rizomas, assim, a propagação in vitro é uma alternativa para a reprodução dessa espécie. Entretanto, mudas produzidas in vitro podem apresentar limitações durante a aclimatização, havendo necessidade de desenvolver uma metodologia para otimizar essa etapa. Além disso, o comércio de flores é marcado por tendências, sendo necessário o desenvolvimento de técnicas para a conservação do germoplasma. Dentre as técnicas que podem ser utilizadas, a criopreservação se destaca por permitir a conservação em longo prazo, no entanto, conhecimento sobre os efeitos na estabilidade genética e aspectos anatômicos das plantas são importantes. Dessa forma, objetivou-se avaliar como o GA3 e a temperatura afetam a germinação de embriões zigóticos e o desenvolvimento in vitro das plântulas, bem como, o efeito de BAP e TDZ na multiplicação, a utilização de telas fotoconversoras durante a aclimatização e o efeito da criopreservação no desenvolvimento, anatomia e estabilidade genética das plantas de estrelícia. Maior germinação (72%) e comprimento da parte aérea (3,14 cm) foram observados com a utilização de 20 μM de GA3. Nessa concentração houve aumento da atividade da redutase do nitrato, sem alteração da estabilidade genética. A temperatura influenciou no crescimento das plantas, o qual foi maior em 25ºC e também na atividade da SOD e APX, que foram mais elevadas com 30ºC. Os resultados demonstraram que 20 μM de BAP proporcionou maior formação de brotações, porém, estes apresentaram redução do comprimento, além de incremento da oxidação do meio de cultura, enquanto que o TDZ não induziu a formação de brotações, nem mesmo redução do comprimento e oxidação do meio de cultura. Durante a aclimatização, a maior taxa de sobrevivência ocorreu para as plantas mantidas na ausência de telas fotoconversoras e para a criopreservação, a desidratação em sílica-gel por 30 minutos foi eficiente para o melhor desenvolvimento das plântulas, não ocorrendo alterações anatômicas e mudanças na estabilidade genética. Dessa forma, recomenda-se para o cultivo in vitro o acréscimo de 20 μM de GA3 ao meio de cultura e a manutenção na temperatura de 25°C para melhor germinação dos embriões zigóticos e desenvolvimento das plântulas, a utilização de 20 μM de BAP para a multiplicação, a ausência de telas fotoconversoras para aclimatização, bem como, a desidratação dos embriões zigóticos por 30 minutos em sílica-gel para a criopreservação.Universidade Federal de LavrasPrograma de Pós-Graduação em Agronomia/Fisiologia VegetalUFLAbrasilDepartamento de AgriculturaPaiva, Patrícia Duarte de OliveiraPaiva, RenatoNery, Fernanda CarlotaCarvalho, Milene Alves de FigueiredoRosa, Stella Dellyzete Veiga Franco daStein, Vanessa CristinaSilva, Diogo Pedrosa Corrêa daFigueiredo, Júnia Rafael Mendonça2020-10-07T18:16:02Z2020-10-07T18:16:02Z2020-10-072018-03-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfFIGUEIREDO, J. R. M. Cultivo in vitro e criopreservação de estrelícia. 2020. 87 p. Tese (Doutorado em Agronomia/Fisiologia Vegetal) – Universidade Federal de Lavras, Lavras, 2018.http://repositorio.ufla.br/jspui/handle/1/43343porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2023-04-26T20:09:23Zoai:localhost:1/43343Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-04-26T20:09:23Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Cultivo in vitro e criopreservação de estrelícia In vitro culture and cryopreservation of strelitzia |
title |
Cultivo in vitro e criopreservação de estrelícia |
spellingShingle |
Cultivo in vitro e criopreservação de estrelícia Figueiredo, Júnia Rafael Mendonça Strelitzia reginae Giberelina Citocinina Aclimatização Criopreservação Gibberellin Cytokinin Acclimatization Cryopreservation Fisiologia Vegetal |
title_short |
Cultivo in vitro e criopreservação de estrelícia |
title_full |
Cultivo in vitro e criopreservação de estrelícia |
title_fullStr |
Cultivo in vitro e criopreservação de estrelícia |
title_full_unstemmed |
Cultivo in vitro e criopreservação de estrelícia |
title_sort |
Cultivo in vitro e criopreservação de estrelícia |
author |
Figueiredo, Júnia Rafael Mendonça |
author_facet |
Figueiredo, Júnia Rafael Mendonça |
author_role |
author |
dc.contributor.none.fl_str_mv |
Paiva, Patrícia Duarte de Oliveira Paiva, Renato Nery, Fernanda Carlota Carvalho, Milene Alves de Figueiredo Rosa, Stella Dellyzete Veiga Franco da Stein, Vanessa Cristina Silva, Diogo Pedrosa Corrêa da |
dc.contributor.author.fl_str_mv |
Figueiredo, Júnia Rafael Mendonça |
dc.subject.por.fl_str_mv |
Strelitzia reginae Giberelina Citocinina Aclimatização Criopreservação Gibberellin Cytokinin Acclimatization Cryopreservation Fisiologia Vegetal |
topic |
Strelitzia reginae Giberelina Citocinina Aclimatização Criopreservação Gibberellin Cytokinin Acclimatization Cryopreservation Fisiologia Vegetal |
description |
Streltzia (Strelitzia reginae) is an ornamental plant used as a cut flower and in landscaping. However, specie propagation is limited by the chemical and physical dormancy of the seeds and by the number of shoots formed, thus, in vitro propagation is an alternative for the reproduction of this species. However, in vitro produced seedlings may present limitations during acclimatization, it is necessary to develop a methodology to optimize these step. In addition, the flower trade is marked by trends, requiring the development of techniques for the conservation of germplasm. Among the techniques that can be used, cryopreservation can be used for the long-term conservation of the species, being necessary to know about the effects on the genetic stability and anatomical aspects of the plants. The objective was to evaluate how GA3 and temperature affect the in vitro development of zygotic embryos, as well as the effect of BAP and TDZ on multiplication, the use of coloured shade nets during acclimatization and the effect of cryopreservation in the development, anatomy and genetic stability of streliztia. Greater germination (72%) and shoot length (3.14 cm) were observed with the use of 20 μM of GA3. In this concentration there was an increase in nitrate reductase activity, without altering the genetic stability. The temperature influenced plant growth, which was higher at 25ºC and also in the SOD and APX activity, which were higher at 30ºC. The results showed that 20 μM BAP provided a higher shoot formation, however, these showed a reduction in length, as well as increased oxidation of the medium since TDZ did not induce shoots. During acclimatization, the highest survival rate occurred for plants maintained without coloured shade nets and during cryopreservation, silica gel dehydration for 30 minutes was efficient for better seedling development, with no anatomical changes and changes in genetic stability. So, it is recommended for the cultivation of zygotic embryos, the addition of 20 μM of GA3 and maintenance at the temperature of 25°C, and for the multiplication 20 μM of BAP is efficient and acclimatization must be performed in the absence of coloured shade nets and for cryopreservation, dehydration should be performed for 30 minutes on silica gel. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-15 2020-10-07T18:16:02Z 2020-10-07T18:16:02Z 2020-10-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
FIGUEIREDO, J. R. M. Cultivo in vitro e criopreservação de estrelícia. 2020. 87 p. Tese (Doutorado em Agronomia/Fisiologia Vegetal) – Universidade Federal de Lavras, Lavras, 2018. http://repositorio.ufla.br/jspui/handle/1/43343 |
identifier_str_mv |
FIGUEIREDO, J. R. M. Cultivo in vitro e criopreservação de estrelícia. 2020. 87 p. Tese (Doutorado em Agronomia/Fisiologia Vegetal) – Universidade Federal de Lavras, Lavras, 2018. |
url |
http://repositorio.ufla.br/jspui/handle/1/43343 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Agronomia/Fisiologia Vegetal UFLA brasil Departamento de Agricultura |
publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Agronomia/Fisiologia Vegetal UFLA brasil Departamento de Agricultura |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1784550050030944256 |