Protoplast production from napier grass and pearl millet triploid hybrids

Detalhes bibliográficos
Autor(a) principal: Timbó, Ana Luiza de Oliveira
Data de Publicação: 2010
Outros Autores: Davide, Lisete Chamma, Pinto, José Eduardo Brasil Pereira, Pereira, Antônio Vander
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500020
http://repositorio.ufla.br/jspui/handle/1/7287
Resumo: The objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied according to the hybrid, the enzymatic solution and the treatment time.
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spelling Protoplast production from napier grass and pearl millet triploid hybridsPennisetum purpureumPennisetum glaucumIn vitro cultivationInterspecific hybridsForageThe objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied according to the hybrid, the enzymatic solution and the treatment time.Editora da Universidade Federal de Lavras2010-10-012015-04-30T13:35:56Z2015-04-30T13:35:56Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500020TIMBÓ, A. L. de O. et al. Protoplast production from napier grass and pearl millet triploid hybrids. Ciência e Agrotecnologia, Lavras, v. 34, n. 5, p. 1219-1223, out. 2010.http://repositorio.ufla.br/jspui/handle/1/7287Ciência e Agrotecnologia v.34 n.5 2010reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLATimbó, Ana Luiza de OliveiraDavide, Lisete ChammaPinto, José Eduardo Brasil PereiraPereira, Antônio Vanderenginfo:eu-repo/semantics/openAccess2016-09-30T18:05:34Zoai:localhost:1/7287Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-09-30T18:05:34Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Protoplast production from napier grass and pearl millet triploid hybrids
title Protoplast production from napier grass and pearl millet triploid hybrids
spellingShingle Protoplast production from napier grass and pearl millet triploid hybrids
Timbó, Ana Luiza de Oliveira
Pennisetum purpureum
Pennisetum glaucum
In vitro cultivation
Interspecific hybrids
Forage
title_short Protoplast production from napier grass and pearl millet triploid hybrids
title_full Protoplast production from napier grass and pearl millet triploid hybrids
title_fullStr Protoplast production from napier grass and pearl millet triploid hybrids
title_full_unstemmed Protoplast production from napier grass and pearl millet triploid hybrids
title_sort Protoplast production from napier grass and pearl millet triploid hybrids
author Timbó, Ana Luiza de Oliveira
author_facet Timbó, Ana Luiza de Oliveira
Davide, Lisete Chamma
Pinto, José Eduardo Brasil Pereira
Pereira, Antônio Vander
author_role author
author2 Davide, Lisete Chamma
Pinto, José Eduardo Brasil Pereira
Pereira, Antônio Vander
author2_role author
author
author
dc.contributor.author.fl_str_mv Timbó, Ana Luiza de Oliveira
Davide, Lisete Chamma
Pinto, José Eduardo Brasil Pereira
Pereira, Antônio Vander
dc.subject.por.fl_str_mv Pennisetum purpureum
Pennisetum glaucum
In vitro cultivation
Interspecific hybrids
Forage
topic Pennisetum purpureum
Pennisetum glaucum
In vitro cultivation
Interspecific hybrids
Forage
description The objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied according to the hybrid, the enzymatic solution and the treatment time.
publishDate 2010
dc.date.none.fl_str_mv 2010-10-01
2015-04-30T13:35:56Z
2015-04-30T13:35:56Z
2015-04-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500020
TIMBÓ, A. L. de O. et al. Protoplast production from napier grass and pearl millet triploid hybrids. Ciência e Agrotecnologia, Lavras, v. 34, n. 5, p. 1219-1223, out. 2010.
http://repositorio.ufla.br/jspui/handle/1/7287
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500020
http://repositorio.ufla.br/jspui/handle/1/7287
identifier_str_mv TIMBÓ, A. L. de O. et al. Protoplast production from napier grass and pearl millet triploid hybrids. Ciência e Agrotecnologia, Lavras, v. 34, n. 5, p. 1219-1223, out. 2010.
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Editora da Universidade Federal de Lavras
publisher.none.fl_str_mv Editora da Universidade Federal de Lavras
dc.source.none.fl_str_mv Ciência e Agrotecnologia v.34 n.5 2010
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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