In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium

Detalhes bibliográficos
Autor(a) principal: Brondani, Gilvano Ebling
Data de Publicação: 2012
Outros Autores: Araujo, Marla Alessandra de, Alcântara, Berenice Kussumoto de, Carvalho, Janice Guedes de, Gonçalves, Antônio Natal, Almeida, Marcílio de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/37295
Resumo: The in vitro organogenesis of woody species plays an essential role in the improvement of forest products by providing saplings with high commercial value. Furthermore, mineral nutrition plays an important role in the induction of organogenic responses. The objective of this study was to evaluate the effects of boron and calcium in the organogenesis of nodal segments from seedlings of Eucalyptus grandis growing under in vitro conditions. The concentration of boron and calcium in MS medium was modified to induce organogenic responses in 45-day-old nodal segments used as explants. After 60 days, the fresh weight, dry weight, ratio of fresh and dry weight, relative water content and relative matter content accumulated by the explants were evaluated. The concentrations of boron and calcium in the culture medium influenced the in vitro organogenic control of Eucalyptus grandis. Reduced combinations of boron and calcium induced callus formation and dry matter accumulation in the explants. A boron concentration of 100% (1.10 mg L-1) combined with 100% (119.950 mg L-1) and 200% (239.900 mg L-1) of calcium, and 200% (2.20 mg L-1) of boron combined with 100% (119.950 mg L-1) of calcium allowed the induction of well-developed buds, which can be used for the regeneration of micro-plants.
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spelling In vitro organogenesis of Eucalyptus grandis: effects of boron and calciumBud inductionCallogenesisTissue cultureMineral nutritionIndução de gemasCalogêneseCultura de tecidoNutrição mineralThe in vitro organogenesis of woody species plays an essential role in the improvement of forest products by providing saplings with high commercial value. Furthermore, mineral nutrition plays an important role in the induction of organogenic responses. The objective of this study was to evaluate the effects of boron and calcium in the organogenesis of nodal segments from seedlings of Eucalyptus grandis growing under in vitro conditions. The concentration of boron and calcium in MS medium was modified to induce organogenic responses in 45-day-old nodal segments used as explants. After 60 days, the fresh weight, dry weight, ratio of fresh and dry weight, relative water content and relative matter content accumulated by the explants were evaluated. The concentrations of boron and calcium in the culture medium influenced the in vitro organogenic control of Eucalyptus grandis. Reduced combinations of boron and calcium induced callus formation and dry matter accumulation in the explants. A boron concentration of 100% (1.10 mg L-1) combined with 100% (119.950 mg L-1) and 200% (239.900 mg L-1) of calcium, and 200% (2.20 mg L-1) of boron combined with 100% (119.950 mg L-1) of calcium allowed the induction of well-developed buds, which can be used for the regeneration of micro-plants.A organogênese in vitro de espécies lenhosas caracteriza-se como fator fundamental para a obtenção de mudas com interesse comercial, gerando aplicações práticas na área do melhoramento florestal. Adicionalmente, a nutrição mineral desempenha papel importante nas respostas de indução organogênica. Objetivou-se avaliar o efeito do boro e cálcio na organogênese de segmentos nodais oriundos de plântulas de Eucalyptus grandis germinadas in vitro. Segmentos nodais com 45 dias após a germinação foram utilizados como explantes. Os explantes foram submetidos a combinações de boro e cálcio em meio de cultura MS, visando induzir diferentes respostas organogênicas. Ao final de 60 dias de cultivo in vitro foram avaliados a matéria fresca, a matéria seca, relação entre matéria fresca e matéria seca, conteúdo relativo de água e conteúdo relativo de matéria acumulada pelos explantes. As concentrações de boro e cálcio no meio de cultura influenciaram o controle organogênico in vitro de Eucalyptus grandis. Combinações reduzidas de boro e cálcio induziram a formação de calo e acúmulo de matéria nos explantes. A concentração de 100% (1,10 mg L-1) de boro combinada com 100% (119,950 mg L-1) e 200% (239,900 mg L-1) de cálcio e, 200% (2,20 mg L-1) de boro combinado com 100% (119,950 mg L-1) de cálcio favoreceram a indução de gemas com desenvolvimento normal, podendo ser utilizadas para a regeneração de microplântulas.Editora da Universidade Estadual de Maringá (EDUEM)2019-10-18T16:35:35Z2019-10-18T16:35:35Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBRONDANI, G. E. et al. In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium. Acta Scientiarum. Agronomy, Maringá, v. 34, n. 4, Oct./Dec. 2012.http://repositorio.ufla.br/jspui/handle/1/37295Acta Scientiarum. Agronomyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessBrondani, Gilvano EblingAraujo, Marla Alessandra deAlcântara, Berenice Kussumoto deCarvalho, Janice Guedes deGonçalves, Antônio NatalAlmeida, Marcílio deeng2019-10-18T16:36:08Zoai:localhost:1/37295Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-10-18T16:36:08Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium
title In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium
spellingShingle In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium
Brondani, Gilvano Ebling
Bud induction
Callogenesis
Tissue culture
Mineral nutrition
Indução de gemas
Calogênese
Cultura de tecido
Nutrição mineral
title_short In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium
title_full In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium
title_fullStr In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium
title_full_unstemmed In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium
title_sort In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium
author Brondani, Gilvano Ebling
author_facet Brondani, Gilvano Ebling
Araujo, Marla Alessandra de
Alcântara, Berenice Kussumoto de
Carvalho, Janice Guedes de
Gonçalves, Antônio Natal
Almeida, Marcílio de
author_role author
author2 Araujo, Marla Alessandra de
Alcântara, Berenice Kussumoto de
Carvalho, Janice Guedes de
Gonçalves, Antônio Natal
Almeida, Marcílio de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Brondani, Gilvano Ebling
Araujo, Marla Alessandra de
Alcântara, Berenice Kussumoto de
Carvalho, Janice Guedes de
Gonçalves, Antônio Natal
Almeida, Marcílio de
dc.subject.por.fl_str_mv Bud induction
Callogenesis
Tissue culture
Mineral nutrition
Indução de gemas
Calogênese
Cultura de tecido
Nutrição mineral
topic Bud induction
Callogenesis
Tissue culture
Mineral nutrition
Indução de gemas
Calogênese
Cultura de tecido
Nutrição mineral
description The in vitro organogenesis of woody species plays an essential role in the improvement of forest products by providing saplings with high commercial value. Furthermore, mineral nutrition plays an important role in the induction of organogenic responses. The objective of this study was to evaluate the effects of boron and calcium in the organogenesis of nodal segments from seedlings of Eucalyptus grandis growing under in vitro conditions. The concentration of boron and calcium in MS medium was modified to induce organogenic responses in 45-day-old nodal segments used as explants. After 60 days, the fresh weight, dry weight, ratio of fresh and dry weight, relative water content and relative matter content accumulated by the explants were evaluated. The concentrations of boron and calcium in the culture medium influenced the in vitro organogenic control of Eucalyptus grandis. Reduced combinations of boron and calcium induced callus formation and dry matter accumulation in the explants. A boron concentration of 100% (1.10 mg L-1) combined with 100% (119.950 mg L-1) and 200% (239.900 mg L-1) of calcium, and 200% (2.20 mg L-1) of boron combined with 100% (119.950 mg L-1) of calcium allowed the induction of well-developed buds, which can be used for the regeneration of micro-plants.
publishDate 2012
dc.date.none.fl_str_mv 2012
2019-10-18T16:35:35Z
2019-10-18T16:35:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BRONDANI, G. E. et al. In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium. Acta Scientiarum. Agronomy, Maringá, v. 34, n. 4, Oct./Dec. 2012.
http://repositorio.ufla.br/jspui/handle/1/37295
identifier_str_mv BRONDANI, G. E. et al. In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium. Acta Scientiarum. Agronomy, Maringá, v. 34, n. 4, Oct./Dec. 2012.
url http://repositorio.ufla.br/jspui/handle/1/37295
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Editora da Universidade Estadual de Maringá (EDUEM)
publisher.none.fl_str_mv Editora da Universidade Estadual de Maringá (EDUEM)
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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